pycom14g03550
ERF Family

Phosphatidylglycerol phosphatidylinositol transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009073
Physical Location & Seq
Forward (+)
2990469 .. 2992748
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03550.2

Sequence Viewer

Length: 468 bp
ATGGCTTTTCAGTTGAAGTTCATGGTCCCCCTCATCCTCCTAGTTTCTCTCTCTCTGGTTGCCCCTCTCATCCAAGCCAAGGACGTCAACTACTGCGATAAGAAGGCTGACTATGATGTCAAGGTCTCCGCGGTTGACATTACTCCTTACCCCGTGGCGAGAGGCAAGCCTGCCAGCTTCAGCATTTCTGCAACTACAGGTACATCGATCTCTGGAGGAAACTTGGTGATTGAGGTTTCGTACTTTGGTTGGCACATCCACAGTGAGACTCATGACCTTTGCGCAGAAACATCTTGCCCTGTTTCAATTGGCGATTTCGTGATTGCCCACTCGCAAGATTTACCCGGATATACTCCACCTGGTTCGTACTCTCTTAAAATGAGGCTGTATGATGGAAACAAACATGAGCTGACATGCATTGCCTTCGATTTTGACATCGGGTTTGTATCATCTGTGGCTGATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E1_DerP2_DerF2

Protein Analysis

156

Amino Acids

16.75

Weight (kDa)

5.13

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AatII GACGTC 1 cut(s) 87
Acc16I TGCGCA 1 cut(s) 283
AccII CGCG 1 cut(s) 131
AciI CCGC 2 cut(s) 129, 131
AcuI CTGAAG 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 3 cut(s) 202, 242, 368
AfiI CCNNNNNNNGG 1 cut(s) 79
AgsI TTSAA 2 cut(s) 16, 306
AjnI CCWGG 1 cut(s) 358
AluBI AGCT 2 cut(s) 177, 409
AluI AGCT 2 cut(s) 177, 409
Alw26I GTCTC 2 cut(s) 130, 260
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 1 cut(s) 25
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 238
AvaII GGWCC 1 cut(s) 25
BccI CCATC 1 cut(s) 386
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 1 cut(s) 360
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 2 cut(s) 130, 260
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 195
Bme1390I CCNGG 2 cut(s) 345, 360
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 2 cut(s) 345, 360
BpmI CTGGAG 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 345
Bsa29I ATCGAT 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 78, 129, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 360
BseCI ATCGAT 1 cut(s) 206
BseDI CCNNGG 3 cut(s) 78, 129, 153
BseGI GGATG 3 cut(s) 33, 69, 255
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMI GCAATG 1 cut(s) 417
Bsh1236I CGCG 1 cut(s) 131
BshVI ATCGAT 1 cut(s) 206
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 2 cut(s) 130, 260
BsmFI GGGAC 1 cut(s) 11
Bso31I GGTCTC 1 cut(s) 130
Bsp143I GATC 1 cut(s) 207
BspACI CCGC 2 cut(s) 129, 131
BspDI ATCGAT 1 cut(s) 206
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 27
BspTNI GGTCTC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 417
BssECI CCNNGG 3 cut(s) 78, 129, 153
BssMI GATC 1 cut(s) 207
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 167, 171, 175
BstDSI CCRYGG 2 cut(s) 129, 153
BstF5I GGATG 3 cut(s) 33, 69, 255
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 1 cut(s) 210
BstMAI GTCTC 2 cut(s) 130, 260
BstMBI GATC 1 cut(s) 207
BstNI CCWGG 1 cut(s) 360
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 2 cut(s) 343, 358
BstSFI CTRYAG 1 cut(s) 195
BstUI CGCG 1 cut(s) 131
Bsu15I ATCGAT 1 cut(s) 206
BsuTUI ATCGAT 1 cut(s) 206
BtgI CCRYGG 2 cut(s) 129, 153
BtsCI GGATG 3 cut(s) 33, 69, 255
BtsIMutI CAGTG 1 cut(s) 268
Cac8I GCNNGC 3 cut(s) 167, 171, 175
CciI TCATGA 1 cut(s) 271
CfoI GCGC 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 25
Cfr42I CCGCGG 1 cut(s) 132
ClaI ATCGAT 1 cut(s) 206
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 3 cut(s) 201, 241, 367
CviAII CATG 4 cut(s) 22, 272, 404, 414
CviJI RGCY 8 cut(s) 5, 77, 107, 169, 177, 385, 409, 458
CviKI_1 RGCY 8 cut(s) 5, 77, 107, 169, 177, 385, 409, 458
CviQI GTAC 3 cut(s) 201, 241, 367
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 4 cut(s) 25, 275, 407, 417
FaiI YATR 7 cut(s) 23, 114, 273, 351, 390, 405, 415
FaqI GGGAC 1 cut(s) 11
FatI CATG 4 cut(s) 21, 271, 403, 413
FokI GGATG 3 cut(s) 20, 56, 242
FspBI CTAG 1 cut(s) 41
FspI TGCGCA 1 cut(s) 283
GlaI GCGC 1 cut(s) 283
GsuI CTGGAG 1 cut(s) 234
HapII CCGG 1 cut(s) 345
HhaI GCGC 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 4 cut(s) 25, 275, 407, 417
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 2 cut(s) 88, 136
HindII GTYRAC 2 cut(s) 88, 136
HinfI GANTC 1 cut(s) 268
HpaII CCGG 1 cut(s) 345
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 88, 136
Hpy188III TCNNGA 3 cut(s) 213, 272, 319
Hpy8I GTNNAC 2 cut(s) 88, 136
HpyAV CCTTC 2 cut(s) 97, 433
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 2 cut(s) 191, 417
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 4 cut(s) 25, 275, 407, 417
HspAI GCGC 1 cut(s) 282
KspI CCGCGG 1 cut(s) 132
Kzo9I GATC 1 cut(s) 207
LpnPI CCDG 9 cut(s) 41, 183, 183, 187, 198, 312, 345, 358, 372
MabI ACCWGGT 1 cut(s) 358
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 84
MalI GATC 1 cut(s) 209
MboI GATC 1 cut(s) 207
MfeI CAATTG 1 cut(s) 306
MluCI AATT 1 cut(s) 306
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 7 cut(s) 41, 47, 75, 155, 209, 226, 375
Mph1103I ATGCAT 1 cut(s) 419
MseI TTAA 2 cut(s) 375, 466
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 345, 360
MunI CAATTG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 360
MvnI CGCG 1 cut(s) 131
NciI CCSGG 1 cut(s) 345
NdeII GATC 1 cut(s) 207
NlaIII CATG 4 cut(s) 25, 275, 407, 417
NlaIV GGNNCC 1 cut(s) 27
NsbI TGCGCA 1 cut(s) 283
NsiI ATGCAT 1 cut(s) 419
NspI RCATGY 1 cut(s) 417
PagI TCATGA 1 cut(s) 271
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 27
PspPI GGNCC 1 cut(s) 25
RsaI GTAC 3 cut(s) 202, 242, 368
RsaNI GTAC 3 cut(s) 201, 241, 367
SacII CCGCGG 1 cut(s) 132
SaqAI TTAA 2 cut(s) 375, 466
Sau3AI GATC 1 cut(s) 207
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 2 cut(s) 345, 360
SetI ASST 8 cut(s) 87, 126, 179, 202, 237, 279, 361, 411
SexAI ACCWGGT 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 195
Sfr303I CCGCGG 1 cut(s) 132
SgrBI CCGCGG 1 cut(s) 132
SinI GGWCC 1 cut(s) 25
Sse9I AATT 1 cut(s) 306
SsiI CCGC 2 cut(s) 129, 131
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 343, 358
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 206, 426
TasI AATT 1 cut(s) 306
Tru1I TTAA 2 cut(s) 375, 466
Tru9I TTAA 2 cut(s) 375, 466
TscAI CASTG 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 10
TspRI CASTG 1 cut(s) 268
VpaK11BI GGWCC 1 cut(s) 25
XceI RCATGY 1 cut(s) 417
XspI CTAG 1 cut(s) 41
ZraI GACGTC 1 cut(s) 85
Zsp2I ATGCAT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.