Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002569

Physical Location & Seq

Genomic Coordinates Forward (+)

2355759 .. 2358241

Gene Structure 2483 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G07440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1236 bp

ATGAATGCTTTAGCTGCAACAAACAGAAACTTCCGTCATGCATCTCGAATCCTGGGTTTGGATTCGAAGATCGAGAGAAGTCTTATGATCCCATTTAGAGAAATCAAGGTTGAGTGTACGATTCCTAAAGACGATGGCACTCTGGTTTCATACATCGGATTTAGGGTTCAACATGACAATGCTCGTGGACCCATGAAAGGTGGAATCAGATATCATCCTGAGGTTGATCCAGATGAAGTTAACGCACTAGCTCAGCTGATGACTTGGAAGACTGCTGTAGCAGATATTCCATATGGTGGTGCTAAAGGTGGAATTGGATGTAGTCCTCGTGACTTGAGTTTGAGCGAGCTTGAGAGGTTGACTCGTGTGTTTACACAGAAGATTCATGATCTTATCGGTATTCATACCGATGTGCCTGCTCCTGATATGGGCACTAACGCTCAAACCATGGCTTGGATTCTTGATGAGTACTCCAAGTTTCATGGTCATTCCCCTGCTGTTGTCACTGGAAAGCCCATTGATCTTGGTGGTTCATTAGGTAGGGAAGCTGCCACAGGTCGTGGTGTAGTATTTGCCACCGAAGCTCTTCTTGCTGAGTACGGGAAATCGATTCAGGGTTTGACATTTGTTATTCAAGGTTTTGGGAATGTTGGAACATGGGCTGCGAAGCTGATCCACGAGAAAGGCGGTAAAGTGGTTGCAGTAAGCGACATTACTGGTGCAATCAGGAACCCTGAAGGTATCGACATCAACGCTCTCATAAAACACAAGGACGCAACTGGAAGTCTCAATGATTTCAATGGTGGAGACGCTATGAATTCAGATGAATTGCTCATTCATGAGTGTGATGTTCTCATTCCATGCGCTCTTGGTGGTGTCCTGAACAAGGAAAATGCTGGAGATGTGAAGGCAAAGTTTATAGTAGAGGCAGCTAACCATCCAACAGATCCAGATGCTGATGAGATTCTGTCGAAGAAAGGAGTGATTATACTTCCAGATATCTACGCAAACGCAGGAGGAGTGACAGTGAGTTACTTCGAGTGGGTGCAGAACATTCAAGGATTCATGTGGGAAGAGGAAAAAGTGAACTTGGAGCTGCAAAAATACATGACTCGAGCCTTTCACAACATCAAGACAATGTGCCATACTCATTCTTGCAACCTCCGTATGGGAGCTTTCACTCTTGGAGTTAACCGAGTCGCTCGAGCCACCCAGTTGCGTGGTTGGGAAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004352 GO:0004353 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0006950 GO:0006970 GO:0006995 GO:0007154 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0010446 GO:0016020 GO:0016491 GO:0016638 GO:0016639 GO:0017076 GO:0030554 GO:0031090 GO:0031667 GO:0031668 GO:0031669 GO:0032553 GO:0032555 GO:0032559 GO:0033554 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0050897 GO:0051716 GO:0055114 GO:0071496 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00471 ko00910 ko01100 ko01200 ko04217 ko04964 map00220 map00250 map00471 map00910 map01100 map01200 map04217 map04964

Pfam Domains

Protein Families

ELFV_dehydrog ELFV_dehydrog_N

Protein Analysis

411

Amino Acids

44.7

Weight (kDa)

6.07

Isoelectric Point (pI)

26.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ELFV_dehydrog_N	PF02812	33 - 159	1.2e-49	Glu/Leu/Phe/Val dehydrogenase, dimerisation domain
ELFV_dehydrog	PF00208	176 - 408	2.7e-83	Glutamate/Leucine/Phenylalanine/Valine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002569)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07440 AT5G07440 AT5G07440
fragaria_vesca	FvH4_5g20340 FvH4_5g20340 FvH4_5g20340 FvH4_5g20340 FvH4_7g26540 FvH4_7g26540
malus_domestica	MD01G1174400.v1.1 MD04G1055700.v1.1 MD07G1241100.v1.1
prunus_persica	Prupe.2G269800_v2.0.a1 Prupe.5G056900_v2.0.a1 Prupe.5G056900_v2.0.a1 Prupe.5G056900_v2.0.a1 Prupe.5G056900_v2.0.a1
pyrus_communis	pycom01g18610 pycom04g04900 pycom07g21730
rosa_chinensis	RchiOBHm_Chr1g0373211 RchiOBHm_Chr7g0205521
rosa_laevigata	RLG00000003372 RLG00000026843
rosa_multiflora	Rmu_sc0000170.1_g000011 Rmu_sc0002237.1_g000014
rosa_roxburghii	Rroxscaffold_3G00252160 Rroxscaffold_4G00284390
rosa_rugosa	Rorug01G0376700 Rorug07G0092000
rosa_samantha	Rh1AG385300 Rh1BG349300 Rh1CG362600 Rh1DG380200 Rh7AG222500 Rh7BG217900 Rh7CG234900 Rh7DG228900
rosa_wichuraiana	Rw1G033990 Rw7G019210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	296, 453
AciI	CCGC	1 cut(s)	687
AclWI	GGATC	4 cut(s)	82, 221, 667, 941
AcsI	RAATTY	1 cut(s)	817
AcuI	CTGAAG	1 cut(s)	756
AfaI	GTAC	3 cut(s)	118, 470, 599
AfiI	CCNNNNNNNGG	7 cut(s)	58, 197, 296, 428, 453, 886, 1168
AgsI	TTSAA	4 cut(s)	170, 635, 799, 1058
AjnI	CCWGG	1 cut(s)	51
AloI	GAACNNNNNNTCC	2 cut(s)	150, 182
Alw26I	GTCTC	2 cut(s)	791, 801
AlwI	GGATC	4 cut(s)	82, 221, 667, 941
AlwNI	CAGNNNCTG	1 cut(s)	956
Ama87I	CYCGRG	2 cut(s)	1113, 1203
ApeKI	GCWGC	5 cut(s)	14, 548, 662, 929, 1096
ApoI	RAATTY	1 cut(s)	817
Asp700I	GAANNNNTTC	1 cut(s)	585
AspLEI	GCGC	1 cut(s)	866
AspS9I	GGNCC	1 cut(s)	188
AsuII	TTCGAA	1 cut(s)	65
AvaI	CYCGRG	2 cut(s)	1113, 1203
AvaII	GGWCC	1 cut(s)	188
AxyI	CCTNAGG	1 cut(s)	219
BaeGI	GKGCMC	1 cut(s)	434
BauI	CACGAG	4 cut(s)	183, 327, 363, 677
BbsI	GAAGAC	1 cut(s)	275
BbvI	GCAGC	4 cut(s)	535, 649, 941, 1083
BccI	CCATC	2 cut(s)	128, 945
BcgI	CGANNNNNNTGC	2 cut(s)	398, 432
BciT130I	CCWGG	1 cut(s)	53
BcoDI	GTCTC	2 cut(s)	791, 801
BfaI	CTAG	1 cut(s)	248
BfmI	CTRYAG	1 cut(s)	276
BisI	GCNGC	5 cut(s)	15, 549, 663, 930, 1097
BlpI	GCTNAGC	1 cut(s)	252
BlsI	GCNGC	5 cut(s)	16, 550, 664, 931, 1098
BmcAI	AGTACT	1 cut(s)	470
Bme1390I	CCNGG	1 cut(s)	53
Bme18I	GGWCC	1 cut(s)	188
BmeT110I	CYCGRG	2 cut(s)	1113, 1203
BmgT120I	GGNCC	1 cut(s)	188
BmiI	GGNNCC	2 cut(s)	190, 731
BmrFI	CCNGG	1 cut(s)	53
BmrI	ACTGGG	1 cut(s)	1207
BmsI	GCATC	2 cut(s)	50, 943
BmuI	ACTGGG	1 cut(s)	1207
BpiI	GAAGAC	1 cut(s)	275
BplI	GAGNNNNNCTC	2 cut(s)	346, 378
BpmI	CTGGAG	1 cut(s)	918
Bpu1102I	GCTNAGC	1 cut(s)	252
Bpu14I	TTCGAA	1 cut(s)	65
BpuEI	CTTGAG	2 cut(s)	355, 371
Bsa29I	ATCGAT	1 cut(s)	608
BsaJI	CCNNGG	2 cut(s)	52, 447
BsaXI	ACNNNNNCTCC	2 cut(s)	1011, 1041
Bsc4I	CCNNNNNNNGG	7 cut(s)	58, 197, 296, 428, 453, 886, 1168
Bse1I	ACTGG	4 cut(s)	511, 721, 784, 1213
Bse21I	CCTNAGG	1 cut(s)	219
BseBI	CCWGG	1 cut(s)	53
BseCI	ATCGAT	1 cut(s)	608
BseDI	CCNNGG	2 cut(s)	52, 447
BseGI	GGATG	3 cut(s)	214, 323, 937
BseLI	CCNNNNNNNGG	7 cut(s)	58, 197, 296, 428, 453, 886, 1168
BseMII	CTCAG	3 cut(s)	210, 266, 585
BseNI	ACTGG	4 cut(s)	511, 721, 784, 1213
BseRI	GAGGAG	1 cut(s)	1032
BseSI	GKGCMC	1 cut(s)	434
BseXI	GCAGC	4 cut(s)	535, 649, 941, 1083
BsgI	GTGCAG	1 cut(s)	1067
BshVI	ATCGAT	1 cut(s)	608
BsiHKCI	CYCGRG	2 cut(s)	1113, 1203
BslI	CCNNNNNNNGG	7 cut(s)	58, 197, 296, 428, 453, 886, 1168
BsmAI	GTCTC	2 cut(s)	791, 801
BsmBI	CGTCTC	1 cut(s)	801
BsmI	GAATGC	1 cut(s)	10
BsoBI	CYCGRG	2 cut(s)	1113, 1203
Bsp119I	TTCGAA	1 cut(s)	65
Bsp1286I	GDGCHC	1 cut(s)	434
Bsp143I	GATC	7 cut(s)	69, 87, 226, 388, 520, 672, 946
Bsp1720I	GCTNAGC	1 cut(s)	252
Bsp19I	CCATGG	1 cut(s)	447
BspACI	CCGC	1 cut(s)	687
BspCNI	CTCAG	3 cut(s)	211, 265, 586
BspDI	ATCGAT	1 cut(s)	608
BspHI	TCATGA	2 cut(s)	385, 838
BspLI	GGNNCC	2 cut(s)	190, 731
BspPI	GGATC	4 cut(s)	82, 221, 667, 941
BspQI	GCTCTTC	1 cut(s)	591
BspT104I	TTCGAA	1 cut(s)	65
BsrI	ACTGG	4 cut(s)	511, 721, 784, 1213
BssECI	CCNNGG	2 cut(s)	52, 447
BssMI	GATC	7 cut(s)	69, 87, 226, 388, 520, 672, 946
BssSI	CACGAG	4 cut(s)	183, 327, 363, 677
BssT1I	CCWWGG	1 cut(s)	447
Bst2BI	CACGAG	4 cut(s)	183, 327, 363, 677
Bst2UI	CCWGG	1 cut(s)	53
Bst4CI	ACNGT	1 cut(s)	1027
Bst6I	CTCTTC	2 cut(s)	591, 1068
BstBI	TTCGAA	1 cut(s)	65
BstC8I	GCNNGC	2 cut(s)	347, 417
BstDEI	CTNAG	3 cut(s)	219, 252, 594
BstDSI	CCRYGG	1 cut(s)	447
BstENI	CCTNNNNNAGG	1 cut(s)	884
BstF5I	GGATG	3 cut(s)	214, 323, 937
BstHHI	GCGC	1 cut(s)	866
BstKTI	GATC	7 cut(s)	72, 90, 229, 391, 523, 675, 949
BstMAI	GTCTC	2 cut(s)	791, 801
BstMBI	GATC	7 cut(s)	69, 87, 226, 388, 520, 672, 946
BstMWI	GCNNNNNNNGC	3 cut(s)	14, 581, 590
BstNI	CCWGG	1 cut(s)	53
BstSCI	CCNGG	1 cut(s)	51
BstSFI	CTRYAG	1 cut(s)	276
BstSLI	GKGCMC	1 cut(s)	434
BstV1I	GCAGC	4 cut(s)	535, 649, 941, 1083
BstV2I	GAAGAC	1 cut(s)	275
BstX2I	RGATCY	1 cut(s)	946
BstXI	CCANNNNNNTGG	1 cut(s)	1220
BstYI	RGATCY	1 cut(s)	946
Bsu15I	ATCGAT	1 cut(s)	608
Bsu36I	CCTNAGG	1 cut(s)	219
BsuTUI	ATCGAT	1 cut(s)	608
BtgI	CCRYGG	1 cut(s)	447
BtsCI	GGATG	3 cut(s)	214, 323, 937
BtsIMutI	CAGTG	2 cut(s)	504, 1032
Cac8I	GCNNGC	2 cut(s)	347, 417
CaiI	CAGNNNCTG	1 cut(s)	956
CciI	TCATGA	2 cut(s)	385, 838
CfoI	GCGC	1 cut(s)	866
Cfr13I	GGNCC	1 cut(s)	188
ClaI	ATCGAT	1 cut(s)	608
CseI	GACGC	2 cut(s)	782, 818
Csp6I	GTAC	3 cut(s)	117, 469, 598
CspCI	CAANNNNNGTGG	2 cut(s)	166, 201
CviQI	GTAC	3 cut(s)	117, 469, 598
DdeI	CTNAG	3 cut(s)	219, 252, 594
DpnI	GATC	7 cut(s)	71, 89, 228, 390, 522, 674, 948
DpnII	GATC	7 cut(s)	69, 87, 226, 388, 520, 672, 946
Eam1104I	CTCTTC	2 cut(s)	591, 1068
EarI	CTCTTC	2 cut(s)	591, 1068
Eco130I	CCWWGG	1 cut(s)	447
Eco32I	GATATC	2 cut(s)	212, 1000
Eco47I	GGWCC	1 cut(s)	188
Eco57I	CTGAAG	1 cut(s)	756
Eco81I	CCTNAGG	1 cut(s)	219
Eco88I	CYCGRG	2 cut(s)	1113, 1203
EcoNI	CCTNNNNNAGG	1 cut(s)	884
EcoRI	GAATTC	1 cut(s)	817
EcoRII	CCWGG	1 cut(s)	51
EcoRV	GATATC	2 cut(s)	212, 1000
EcoT14I	CCWWGG	1 cut(s)	447
EcoT22I	ATGCAT	1 cut(s)	43
ErhI	CCWWGG	1 cut(s)	447
Esp3I	CGTCTC	1 cut(s)	801
FalI	AAGNNNNNCTT	2 cut(s)	573, 605
FauNDI	CATATG	1 cut(s)	292
Fnu4HI	GCNGC	5 cut(s)	15, 549, 663, 930, 1097
FokI	GGATG	3 cut(s)	201, 330, 924
Fsp4HI	GCNGC	5 cut(s)	15, 549, 663, 930, 1097
FspBI	CTAG	1 cut(s)	248
GlaI	GCGC	1 cut(s)	865
GluI	GCNGC	5 cut(s)	15, 549, 663, 930, 1097
GsuI	CTGGAG	1 cut(s)	918
HgaI	GACGC	2 cut(s)	782, 818
HhaI	GCGC	1 cut(s)	866
Hin6I	GCGC	1 cut(s)	864
HinP1I	GCGC	1 cut(s)	864
HincII	GTYRAC	3 cut(s)	241, 360, 1192
HindII	GTYRAC	3 cut(s)	241, 360, 1192
HindIII	AAGCTT	1 cut(s)	1230
HpaI	GTTAAC	2 cut(s)	241, 1192
Hpy166II	GTNNAC	7 cut(s)	117, 188, 241, 360, 372, 1087, 1192
Hpy188I	TCNGA	3 cut(s)	158, 209, 823
Hpy8I	GTNNAC	7 cut(s)	117, 188, 241, 360, 372, 1087, 1192
HpyAV	CCTTC	2 cut(s)	731, 901
HpyCH4III	ACNGT	1 cut(s)	1027
HpyCH4V	TGCA	7 cut(s)	17, 41, 701, 722, 1048, 1099, 1158
HpyF10VI	GCNNNNNNNGC	3 cut(s)	14, 581, 590
HpyF3I	CTNAG	3 cut(s)	219, 252, 594
HspAI	GCGC	1 cut(s)	864
KspAI	GTTAAC	2 cut(s)	241, 1192
Kzo9I	GATC	7 cut(s)	69, 87, 226, 388, 520, 672, 946
LguI	GCTCTTC	1 cut(s)	591
LmnI	GCTCC	3 cut(s)	424, 1093, 1172
Lsp1109I	GCAGC	4 cut(s)	535, 649, 941, 1083
LweI	GCATC	2 cut(s)	50, 943
MaeI	CTAG	1 cut(s)	248
MaeIII	GTNAC	4 cut(s)	329, 502, 1021, 1031
MalI	GATC	7 cut(s)	71, 89, 228, 390, 522, 674, 948
MboI	GATC	7 cut(s)	69, 87, 226, 388, 520, 672, 946
MboII	GAAGA	6 cut(s)	79, 280, 391, 578, 985, 1085
MflI	RGATCY	1 cut(s)	946
MhlI	GDGCHC	1 cut(s)	434
MluCI	AATT	3 cut(s)	312, 817, 827
MlyI	GAGTC	3 cut(s)	355, 1105, 1206
MmeI	TCCRAC	2 cut(s)	631, 965
MnlI	CCTC	7 cut(s)	214, 336, 348, 919, 1010, 1069, 1172
Mph1103I	ATGCAT	1 cut(s)	43
MroXI	GAANNNNTTC	1 cut(s)	585
MseI	TTAA	3 cut(s)	240, 1191, 1234
MslI	CAYNNNNRTG	3 cut(s)	177, 408, 843
MspA1I	CMGCKG	1 cut(s)	256
MspR9I	CCNGG	1 cut(s)	53
Mva1269I	GAATGC	1 cut(s)	10
MvaI	CCWGG	1 cut(s)	53
MwoI	GCNNNNNNNGC	3 cut(s)	14, 581, 590
NcoI	CCATGG	1 cut(s)	447
NdeI	CATATG	1 cut(s)	292
NdeII	GATC	7 cut(s)	69, 87, 226, 388, 520, 672, 946
NlaIV	GGNNCC	2 cut(s)	190, 731
NmuCI	GTSAC	3 cut(s)	329, 502, 1021
NsiI	ATGCAT	1 cut(s)	43
NspV	TTCGAA	1 cut(s)	65
PaeR7I	CTCGAG	2 cut(s)	1113, 1203
PagI	TCATGA	2 cut(s)	385, 838
PciSI	GCTCTTC	1 cut(s)	591
PctI	GAATGC	1 cut(s)	10
PdmI	GAANNNNTTC	1 cut(s)	585
PfeI	GAWTC	9 cut(s)	48, 62, 121, 204, 382, 457, 610, 964, 1062
PflMI	CCANNNNNTGG	2 cut(s)	296, 453
PkrI	GCNGC	5 cut(s)	16, 550, 664, 931, 1098
PleI	GAGTC	3 cut(s)	355, 1105, 1205
PpsI	GAGTC	3 cut(s)	355, 1105, 1205
Psp6I	CCWGG	1 cut(s)	51
PspGI	CCWGG	1 cut(s)	51
PspN4I	GGNNCC	2 cut(s)	190, 731
PspPI	GGNCC	1 cut(s)	188
PspXI	VCTCGAGB	2 cut(s)	1113, 1203
PstNI	CAGNNNCTG	1 cut(s)	956
PsuI	RGATCY	1 cut(s)	946
PvuII	CAGCTG	1 cut(s)	256
RsaI	GTAC	3 cut(s)	118, 470, 599
RsaNI	GTAC	3 cut(s)	117, 469, 598
RseI	CAYNNNNRTG	3 cut(s)	177, 408, 843
SapI	GCTCTTC	1 cut(s)	591
SaqAI	TTAA	3 cut(s)	240, 1191, 1234
SatI	GCNGC	5 cut(s)	15, 549, 663, 930, 1097
Sau3AI	GATC	7 cut(s)	69, 87, 226, 388, 520, 672, 946
Sau96I	GGNCC	1 cut(s)	188
ScaI	AGTACT	1 cut(s)	470
SchI	GAGTC	3 cut(s)	355, 1105, 1206
ScrFI	CCNGG	1 cut(s)	53
SduI	GDGCHC	1 cut(s)	434
SfaNI	GCATC	2 cut(s)	50, 943
SfcI	CTRYAG	1 cut(s)	276
Sfr274I	CTCGAG	2 cut(s)	1113, 1203
SfuI	TTCGAA	1 cut(s)	65
SinI	GGWCC	1 cut(s)	188
SlaI	CTCGAG	2 cut(s)	1113, 1203
SmiMI	CAYNNNNRTG	3 cut(s)	177, 408, 843
SmlI	CTYRAG	4 cut(s)	334, 350, 1113, 1203
SmoI	CTYRAG	4 cut(s)	334, 350, 1113, 1203
Sse9I	AATT	3 cut(s)	312, 817, 827
SsiI	CCGC	1 cut(s)	687
SspMI	CTAG	1 cut(s)	248
StyD4I	CCNGG	1 cut(s)	51
StyI	CCWWGG	1 cut(s)	447
TaaI	ACNGT	1 cut(s)	1027
TaqI	TCGA	9 cut(s)	46, 65, 72, 608, 744, 971, 1038, 1114, 1204
TasI	AATT	3 cut(s)	312, 817, 827
TatI	WGTACW	1 cut(s)	468
TfiI	GAWTC	9 cut(s)	48, 62, 121, 204, 382, 457, 610, 964, 1062
Tru1I	TTAA	3 cut(s)	240, 1191, 1234
Tru9I	TTAA	3 cut(s)	240, 1191, 1234
TscAI	CASTG	2 cut(s)	511, 1032
TseFI	GTSAC	3 cut(s)	329, 502, 1021
TseI	GCWGC	5 cut(s)	14, 548, 662, 929, 1096
Tsp45I	GTSAC	3 cut(s)	329, 502, 1021
TspGWI	ACGGA	2 cut(s)	23, 1154
TspRI	CASTG	2 cut(s)	511, 1032
Van91I	CCANNNNNTGG	2 cut(s)	296, 453
VpaK11BI	GGWCC	1 cut(s)	188
XagI	CCTNNNNNAGG	1 cut(s)	884
XapI	RAATTY	1 cut(s)	817
XhoI	CTCGAG	2 cut(s)	1113, 1203
XmnI	GAANNNNTTC	1 cut(s)	585
XspI	CTAG	1 cut(s)	248
ZrmI	AGTACT	1 cut(s)	470
Zsp2I	ATGCAT	1 cut(s)	43

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.