Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002569

Physical Location & Seq

Genomic Coordinates Forward (+)

6740123 .. 6744354

Gene Structure 4232 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1055700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1236 bp

ATGAATGCTCTTGCAGCCACCAACCGCAACTTTCGACATGCAGCTCGCATTCTTGGGCTGGATTCCAAGCTTGAGAAAAGCCTTTTGATTCCCTTCAGAGAAATCAAAGTTGAGTGTACTATTCCGAAAGATGATGGCACTCTGGTATCCTATGTTGGATTCAGAATCCAGCATGACAATGCCCGTGGCCCAATGAAAGGGGGAATCCGTTACCATCCTGAGGTTGACCCAGATGAAGTGAATGCTCTAGCCCAGCTAATGACATGGAAGACAGCTGTAGTAAACATTCCTTATGGTGGAGCTAAGGGTGGGATTGGCTGCAACCCGAGGGACTTGAGTGTCAGTGAGTTAGAACGTTTAACTCGTGTTTTTACTCAGAAGATCCATGATCTCATTGGAATTCACAGGGATGTTCCTGCCCCTGACATGGGAACTAATTCCCAGACAATGGCTTGGATTCTTGACGAGTACTCAAAGTTTCATGGGCATTCACCTGCTGTTGTGACAGGAAAACCGATTGATCTTGGGGGTTCACTTGGAAGGGAGTCTGCGACTGGACTTGGGGTGGTTTTTGCAACCGAGTCTTTACTTGGTGAATACGGAAAGTCAATTTCCGGTATGAAGTTTGTTATACAGGGTTTCGGAAATGTGGGCTCGTGGGCAGCCAAGTTGATCCATGATAGAGGGGGTCATGTCATTGCTGTTAGTGACATCACAGGTGCAATTAAAAACCCTGCTGGAATCAATATCCCAGATCTTCTGAAGCACAAAGATAGCAATGGAAGTTTGAAGGAATTCCAAGGTGGAGATGCTATGGACCCAAATGATTTGCTTGTTCATGAATGTGATGTTCTGATTCCATGTGCCTTAGGAGGAGTTATTAACAAGGAAAATGCAGCTGATGTGAAGGCAAAATTCATAATAGAAGCTGCAAATCATCCAACTGACCCAGAAGCAGACGAGATTTTGTCCAATAAGGGAGTTGTTATTCTCCCTGACATATATGCAAATGCTGGCGGTGTGACAGTGAGCTACTTTGAATGGGTTCAGAATATTCAAGGATTTATGTGGGAAGAAGACAAGGTTAACTGTGAGCTTAAAAAGTACATGCACAAGGCCTTCCTTGACATCAAGGCATTGTGTCAAACTCATGACTGCAGCTTGCGAATGGGAGCTTTCACTCTCGGGGTGAACCGAGTCGCACGTGCTACACTCTTGAGGGGTTGGGAAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004352 GO:0004353 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0006950 GO:0006970 GO:0006995 GO:0007154 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0010446 GO:0016020 GO:0016491 GO:0016638 GO:0016639 GO:0017076 GO:0030554 GO:0031090 GO:0031667 GO:0031668 GO:0031669 GO:0032553 GO:0032555 GO:0032559 GO:0033554 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0050897 GO:0051716 GO:0055114 GO:0071496 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00471 ko00910 ko01100 ko01200 ko04217 ko04964 map00220 map00250 map00471 map00910 map01100 map01200 map04217 map04964

Pfam Domains

Protein Families

ELFV_dehydrog ELFV_dehydrog_N

Protein Analysis

412

Amino Acids

44.65

Weight (kDa)

6.17

Isoelectric Point (pI)

26.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ELFV_dehydrog_N	PF02812	33 - 159	3.2e-50	Glu/Leu/Phe/Val dehydrogenase, dimerisation domain
ELFV_dehydrog	PF00208	176 - 408	9.2e-80	Glutamate/Leucine/Phenylalanine/Valine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002569)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07440 AT5G07440 AT5G07440
fragaria_vesca	FvH4_5g20340 FvH4_5g20340 FvH4_5g20340 FvH4_5g20340 FvH4_7g26540 FvH4_7g26540
malus_domestica	MD01G1174400.v1.1 MD04G1055700.v1.1 MD07G1241100.v1.1
prunus_persica	Prupe.2G269800_v2.0.a1 Prupe.5G056900_v2.0.a1 Prupe.5G056900_v2.0.a1 Prupe.5G056900_v2.0.a1 Prupe.5G056900_v2.0.a1
pyrus_communis	pycom01g18610 pycom04g04900 pycom07g21730
rosa_chinensis	RchiOBHm_Chr1g0373211 RchiOBHm_Chr7g0205521
rosa_laevigata	RLG00000003372 RLG00000026843
rosa_multiflora	Rmu_sc0000170.1_g000011 Rmu_sc0002237.1_g000014
rosa_roxburghii	Rroxscaffold_3G00252160 Rroxscaffold_4G00284390
rosa_rugosa	Rorug01G0376700 Rorug07G0092000
rosa_samantha	Rh1AG385300 Rh1BG349300 Rh1CG362600 Rh1DG380200 Rh7AG222500 Rh7BG217900 Rh7CG234900 Rh7DG228900
rosa_wichuraiana	Rw1G033990 Rw7G019210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	502
AasI	GACNNNNNNGTC	1 cut(s)	338
Acc36I	ACCTGC	1 cut(s)	502
AccB7I	CCANNNNNTGG	1 cut(s)	448
AciI	CCGC	2 cut(s)	25, 1017
AclI	AACGTT	1 cut(s)	355
AclWI	GGATC	2 cut(s)	376, 667
AcsI	RAATTY	3 cut(s)	399, 794, 914
AcuI	CTGAAG	2 cut(s)	79, 782
AcvI	CACGTG	1 cut(s)	1205
AfaI	GTAC	3 cut(s)	118, 470, 1106
AfiI	CCNNNNNNNGG	6 cut(s)	197, 220, 296, 427, 428, 448
AgsI	TTSAA	3 cut(s)	790, 1040, 1058
AlwI	GGATC	2 cut(s)	376, 667
Ama87I	CYCGRG	2 cut(s)	325, 1184
AoxI	GGCC	2 cut(s)	187, 1116
ApeKI	GCWGC	7 cut(s)	14, 41, 318, 662, 896, 929, 1158
ApoI	RAATTY	3 cut(s)	399, 794, 914
Asp700I	GAANNNNTTC	3 cut(s)	436, 794, 1044
AspS9I	GGNCC	2 cut(s)	188, 817
AsuHPI	GGTGA	3 cut(s)	483, 605, 1201
AvaI	CYCGRG	2 cut(s)	325, 1184
AvaII	GGWCC	1 cut(s)	817
AxyI	CCTNAGG	2 cut(s)	219, 868
BaeI	ACNNNNGTAYC	2 cut(s)	129, 162
BanII	GRGCYC	1 cut(s)	656
BauI	CACGAG	2 cut(s)	363, 655
BbrPI	CACGTG	1 cut(s)	1205
BbsI	GAAGAC	2 cut(s)	275, 1083
BbvI	GCAGC	7 cut(s)	26, 53, 305, 674, 908, 916, 1170
BccI	CCATC	2 cut(s)	128, 222
BciVI	GTATCC	1 cut(s)	157
BfaI	CTAG	1 cut(s)	248
BfmI	CTRYAG	2 cut(s)	276, 1156
BfuAI	ACCTGC	1 cut(s)	502
BfuI	GTATCC	1 cut(s)	157
BglII	AGATCT	1 cut(s)	754
BisI	GCNGC	7 cut(s)	15, 42, 319, 663, 897, 930, 1159
BlsI	GCNGC	7 cut(s)	16, 43, 320, 664, 898, 931, 1160
BmcAI	AGTACT	1 cut(s)	470
Bme18I	GGWCC	1 cut(s)	817
BmeT110I	CYCGRG	2 cut(s)	325, 1184
BmgT120I	GGNCC	2 cut(s)	188, 817
BmiI	GGNNCC	1 cut(s)	819
BmsI	GCATC	1 cut(s)	799
BpiI	GAAGAC	2 cut(s)	275, 1083
Bpu10I	CCTNAGC	1 cut(s)	303
BpuEI	CTTGAG	2 cut(s)	92, 355
BsaAI	YACGTR	1 cut(s)	1205
BsaJI	CCNNGG	3 cut(s)	184, 326, 799
BsaWI	WCCGGW	1 cut(s)	614
Bsc4I	CCNNNNNNNGG	6 cut(s)	197, 220, 296, 427, 428, 448
Bse1I	ACTGG	1 cut(s)	559
Bse21I	CCTNAGG	2 cut(s)	219, 868
Bse3DI	GCAATG	2 cut(s)	696, 784
BseDI	CCNNGG	3 cut(s)	184, 326, 799
BseGI	GGATG	3 cut(s)	214, 415, 937
BseLI	CCNNNNNNNGG	6 cut(s)	197, 220, 296, 427, 428, 448
BseMI	GCAATG	2 cut(s)	696, 784
BseMII	CTCAG	2 cut(s)	210, 389
BseNI	ACTGG	1 cut(s)	559
BseRI	GAGGAG	1 cut(s)	888
BseXI	GCAGC	7 cut(s)	26, 53, 305, 674, 908, 916, 1170
BseYI	CCCAGC	1 cut(s)	252
BshFI	GGCC	2 cut(s)	189, 1118
BsiHKCI	CYCGRG	2 cut(s)	325, 1184
BsiSI	CCGG	1 cut(s)	615
BslFI	GGGAC	1 cut(s)	344
BslI	CCNNNNNNNGG	6 cut(s)	197, 220, 296, 427, 428, 448
BsmFI	GGGAC	1 cut(s)	344
BsmI	GAATGC	4 cut(s)	10, 48, 247, 487
BsnI	GGCC	2 cut(s)	189, 1118
BsoBI	CYCGRG	2 cut(s)	325, 1184
Bsp1286I	GDGCHC	1 cut(s)	656
Bsp143I	GATC	5 cut(s)	381, 388, 520, 672, 754
BspACI	CCGC	2 cut(s)	25, 1017
BspANI	GGCC	2 cut(s)	189, 1118
BspCNI	CTCAG	2 cut(s)	211, 388
BspHI	TCATGA	2 cut(s)	838, 1150
BspLI	GGNNCC	1 cut(s)	819
BspMAI	CTGCAG	1 cut(s)	1160
BspMI	ACCTGC	1 cut(s)	502
BspPI	GGATC	2 cut(s)	376, 667
BsrDI	GCAATG	2 cut(s)	696, 784
BsrI	ACTGG	1 cut(s)	559
BssECI	CCNNGG	3 cut(s)	184, 326, 799
BssMI	GATC	5 cut(s)	381, 388, 520, 672, 754
BssSI	CACGAG	2 cut(s)	363, 655
BssT1I	CCWWGG	1 cut(s)	799
Bst2BI	CACGAG	2 cut(s)	363, 655
Bst4CI	ACNGT	2 cut(s)	1027, 1091
BstBAI	YACGTR	1 cut(s)	1205
BstC8I	GCNNGC	3 cut(s)	46, 1015, 1163
BstDEI	CTNAG	4 cut(s)	219, 303, 375, 868
BstDSI	CCRYGG	1 cut(s)	184
BstF5I	GGATG	3 cut(s)	214, 415, 937
BstKTI	GATC	5 cut(s)	384, 391, 523, 675, 757
BstMBI	GATC	5 cut(s)	381, 388, 520, 672, 754
BstMWI	GCNNNNNNNGC	1 cut(s)	14
BstNSI	RCATGY	2 cut(s)	41, 1111
BstSFI	CTRYAG	2 cut(s)	276, 1156
BstV1I	GCAGC	7 cut(s)	26, 53, 305, 674, 908, 916, 1170
BstV2I	GAAGAC	2 cut(s)	275, 1083
BstX2I	RGATCY	2 cut(s)	381, 754
BstYI	RGATCY	2 cut(s)	381, 754
Bsu36I	CCTNAGG	2 cut(s)	219, 868
BsuI	GTATCC	1 cut(s)	157
BsuRI	GGCC	2 cut(s)	189, 1118
BtgI	CCRYGG	1 cut(s)	184
BtsCI	GGATG	3 cut(s)	214, 415, 937
BtsIMutI	CAGTG	2 cut(s)	349, 1032
BveI	ACCTGC	1 cut(s)	502
Cac8I	GCNNGC	3 cut(s)	46, 1015, 1163
CciI	TCATGA	2 cut(s)	838, 1150
Cfr13I	GGNCC	2 cut(s)	188, 817
Csp6I	GTAC	3 cut(s)	117, 469, 1105
CspCI	CAANNNNNGTGG	2 cut(s)	166, 201
CviQI	GTAC	3 cut(s)	117, 469, 1105
DdeI	CTNAG	4 cut(s)	219, 303, 375, 868
DpnI	GATC	5 cut(s)	383, 390, 522, 674, 756
DpnII	GATC	5 cut(s)	381, 388, 520, 672, 754
DrdI	GACNNNNNNGTC	1 cut(s)	338
DseDI	GACNNNNNNGTC	1 cut(s)	338
Eco130I	CCWWGG	1 cut(s)	799
Eco147I	AGGCCT	1 cut(s)	1118
Eco24I	GRGCYC	1 cut(s)	656
Eco47I	GGWCC	1 cut(s)	817
Eco57I	CTGAAG	2 cut(s)	79, 782
Eco72I	CACGTG	1 cut(s)	1205
Eco81I	CCTNAGG	2 cut(s)	219, 868
Eco88I	CYCGRG	2 cut(s)	325, 1184
EcoRI	GAATTC	2 cut(s)	399, 794
EcoT14I	CCWWGG	1 cut(s)	799
EcoT38I	GRGCYC	1 cut(s)	656
ErhI	CCWWGG	1 cut(s)	799
FaqI	GGGAC	1 cut(s)	344
Fnu4HI	GCNGC	7 cut(s)	15, 42, 319, 663, 897, 930, 1159
FokI	GGATG	3 cut(s)	201, 422, 924
FriOI	GRGCYC	1 cut(s)	656
Fsp4HI	GCNGC	7 cut(s)	15, 42, 319, 663, 897, 930, 1159
FspBI	CTAG	1 cut(s)	248
GluI	GCNGC	7 cut(s)	15, 42, 319, 663, 897, 930, 1159
GsaI	CCCAGC	1 cut(s)	256
HaeIII	GGCC	2 cut(s)	189, 1118
HapII	CCGG	1 cut(s)	615
HincII	GTYRAC	2 cut(s)	226, 1087
HindII	GTYRAC	2 cut(s)	226, 1087
HindIII	AAGCTT	1 cut(s)	68
HpaI	GTTAAC	1 cut(s)	1087
HpaII	CCGG	1 cut(s)	615
HphI	GGTGA	3 cut(s)	483, 605, 1201
Hpy166II	GTNNAC	6 cut(s)	117, 226, 283, 533, 1087, 1192
Hpy188I	TCNGA	8 cut(s)	98, 126, 164, 378, 644, 762, 855, 1050
Hpy188III	TCNNGA	5 cut(s)	218, 461, 839, 1151, 1216
Hpy8I	GTNNAC	6 cut(s)	117, 226, 283, 533, 1087, 1192
HpyAV	CCTTC	5 cut(s)	103, 534, 784, 901, 1129
HpyCH4III	ACNGT	2 cut(s)	1027, 1091
HpyCH4IV	ACGT	2 cut(s)	355, 1204
HpyF10VI	GCNNNNNNNGC	1 cut(s)	14
HpyF3I	CTNAG	4 cut(s)	219, 303, 375, 868
HpySE526I	ACGT	2 cut(s)	355, 1204
KspAI	GTTAAC	1 cut(s)	1087
Kzo9I	GATC	5 cut(s)	381, 388, 520, 672, 754
LmnI	GCTCC	2 cut(s)	299, 1172
Lsp1109I	GCAGC	7 cut(s)	26, 53, 305, 674, 908, 916, 1170
LweI	GCATC	1 cut(s)	799
MaeI	CTAG	1 cut(s)	248
MaeII	ACGT	2 cut(s)	355, 1204
MaeIII	GTNAC	4 cut(s)	209, 502, 707, 1021
MalI	GATC	5 cut(s)	383, 390, 522, 674, 756
MboI	GATC	5 cut(s)	381, 388, 520, 672, 754
MboII	GAAGA	5 cut(s)	280, 391, 749, 1085, 1088
MflI	RGATCY	2 cut(s)	381, 754
MhlI	GDGCHC	1 cut(s)	656
MluCI	AATT	6 cut(s)	399, 436, 609, 723, 794, 914
MlyI	GAGTC	3 cut(s)	554, 590, 1206
MmeI	TCCRAC	2 cut(s)	136, 965
MnlI	CCTC	5 cut(s)	214, 321, 677, 866, 1212
MroXI	GAANNNNTTC	3 cut(s)	436, 794, 1044
MseI	TTAA	5 cut(s)	359, 726, 882, 1086, 1098
MslI	CAYNNNNRTG	3 cut(s)	177, 408, 843
MspA1I	CMGCKG	2 cut(s)	275, 899
MspI	CCGG	1 cut(s)	615
Mva1269I	GAATGC	4 cut(s)	10, 48, 247, 487
MwoI	GCNNNNNNNGC	1 cut(s)	14
NdeII	GATC	5 cut(s)	381, 388, 520, 672, 754
NlaIV	GGNNCC	1 cut(s)	819
NmuCI	GTSAC	3 cut(s)	502, 707, 1021
NspI	RCATGY	2 cut(s)	41, 1111
PagI	TCATGA	2 cut(s)	838, 1150
PaqCI	CACCTGC	1 cut(s)	502
PceI	AGGCCT	1 cut(s)	1118
PcsI	WCGNNNNNNNCGW	1 cut(s)	361
PctI	GAATGC	4 cut(s)	10, 48, 247, 487
PdmI	GAANNNNTTC	3 cut(s)	436, 794, 1044
PfeI	GAWTC	8 cut(s)	62, 88, 159, 165, 204, 457, 741, 856
PflMI	CCANNNNNTGG	1 cut(s)	448
PkrI	GCNGC	7 cut(s)	16, 43, 320, 664, 898, 931, 1160
PleI	GAGTC	3 cut(s)	553, 589, 1205
PmaCI	CACGTG	1 cut(s)	1205
PmlI	CACGTG	1 cut(s)	1205
PpsI	GAGTC	3 cut(s)	553, 589, 1205
Ppu21I	YACGTR	1 cut(s)	1205
Psp1406I	AACGTT	1 cut(s)	355
PspCI	CACGTG	1 cut(s)	1205
PspFI	CCCAGC	1 cut(s)	252
PspN4I	GGNNCC	1 cut(s)	819
PspPI	GGNCC	2 cut(s)	188, 817
PstI	CTGCAG	1 cut(s)	1160
PsuI	RGATCY	2 cut(s)	381, 754
PvuII	CAGCTG	2 cut(s)	275, 899
RsaI	GTAC	3 cut(s)	118, 470, 1106
RsaNI	GTAC	3 cut(s)	117, 469, 1105
RseI	CAYNNNNRTG	3 cut(s)	177, 408, 843
SaqAI	TTAA	5 cut(s)	359, 726, 882, 1086, 1098
SatI	GCNGC	7 cut(s)	15, 42, 319, 663, 897, 930, 1159
Sau3AI	GATC	5 cut(s)	381, 388, 520, 672, 754
Sau96I	GGNCC	2 cut(s)	188, 817
ScaI	AGTACT	1 cut(s)	470
SchI	GAGTC	3 cut(s)	554, 590, 1206
SduI	GDGCHC	1 cut(s)	656
SfaNI	GCATC	1 cut(s)	799
SfcI	CTRYAG	2 cut(s)	276, 1156
SinI	GGWCC	1 cut(s)	817
SmiMI	CAYNNNNRTG	3 cut(s)	177, 408, 843
SmlI	CTYRAG	3 cut(s)	71, 334, 1216
SmoI	CTYRAG	3 cut(s)	71, 334, 1216
Sse9I	AATT	6 cut(s)	399, 436, 609, 723, 794, 914
SseBI	AGGCCT	1 cut(s)	1118
SsiI	CCGC	2 cut(s)	25, 1017
SspI	AATATT	1 cut(s)	1054
SspMI	CTAG	1 cut(s)	248
StuI	AGGCCT	1 cut(s)	1118
StyI	CCWWGG	1 cut(s)	799
TaaI	ACNGT	2 cut(s)	1027, 1091
TaiI	ACGT	2 cut(s)	358, 1207
TaqI	TCGA	1 cut(s)	34
TasI	AATT	6 cut(s)	399, 436, 609, 723, 794, 914
TatI	WGTACW	3 cut(s)	116, 468, 1104
TfiI	GAWTC	8 cut(s)	62, 88, 159, 165, 204, 457, 741, 856
Tru1I	TTAA	5 cut(s)	359, 726, 882, 1086, 1098
Tru9I	TTAA	5 cut(s)	359, 726, 882, 1086, 1098
TscAI	CASTG	2 cut(s)	349, 1032
TseFI	GTSAC	3 cut(s)	502, 707, 1021
TseI	GCWGC	7 cut(s)	14, 41, 318, 662, 896, 929, 1158
Tsp45I	GTSAC	3 cut(s)	502, 707, 1021
TspDTI	ATGAA	8 cut(s)	17, 209, 249, 470, 635, 827, 855, 907
TspGWI	ACGGA	2 cut(s)	197, 615
TspRI	CASTG	2 cut(s)	349, 1032
Van91I	CCANNNNNTGG	1 cut(s)	448
VpaK11BI	GGWCC	1 cut(s)	817
XapI	RAATTY	3 cut(s)	399, 794, 914
XceI	RCATGY	2 cut(s)	41, 1111
XcmI	CCANNNNNNNNNTGG	1 cut(s)	392
XmnI	GAANNNNTTC	3 cut(s)	436, 794, 1044
XspI	CTAG	1 cut(s)	248
ZrmI	AGTACT	1 cut(s)	470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.