AT5G08020

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009976
Physical Location & Seq
Forward (+)
2571957 .. 2575061
3105 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08020.1

Sequence Viewer

Length: 1815 bp
ATGGAGAACTCAGTGACCCAAGATGGTATCGCGACAGTATTAGCCAATCAATCCCTTGATTCCTCCTCTGTTAGACCCGAAATTGTTGTTCAAGTCGTGGATCTTAAGCCTGCTGGAAATCGATATACGTTTAGTGCCAATGATGGAAAGATGAAGATTAAAGCAATGTTACCAGCGACTTTGACTTCTGATATCATCTCTGGAAAGATTCAGAATCTGGGTCTTATTCGTCTCCTTGAGTATACCGTCAATGATATTCCAGGCAAATCAGAAGAAAAATATATGCTTATAACAAAATGTGAGGCTGTTGCTTCTGCACTTGACTCAGAGATTAAGGCGGAGATTAAAGCTTCCACAGGCATAATGTTGAAACCCAAGCATGAGTTTGTTGCCAAATCAGCTTCACAGATTATCAATGAGCAAAGGGGAAATGCTGCACCTGCTGCAAGAATGGCTATGACCAGAAGGGTTCATCCTCTTGTGTCCTTGAACCCTTATCAAGGAAGTTGGACTATTAAAGTTCGTGTCACTAACAAAGGAGTCATGAGAACTTACAAAAATGCCAGAGGAGAAGGATGTGTGTTTAATGTGGAACTAACTGATGAGGAAGGAACACAGATTCAGGCAACAATGTTTAACGCAGCTGCAAGGAAGTTTTATGACAGATTCGAGATGGGAAAGGTGTACTATATATCCAGGGGATCACTTAAGCTTGCTAATAAGCAGTTCAAGACGGTTCAGAACGATTATGAGATGACCCTAAATGAGAATTCTGAGGTTGAAGAAGCTAGTAATGAAGAAATGTTTACCCCTGAAACTAAATTCAACTTTGTACCCATTGATGAGTTGGGTACATACGTGAATCAGAAAGACCTTATTGATGTTATCGGTGTTGTTCAAAGTGTTTCTCCTACCATGAGCATTAGAAGGAAGAACGACAATGAGATGATCCCAAAGAGAGATATAACTTTGGCTGATGAGACAAAGAAAACTGTTGTGGTGTCCCTTTGGAATGATCTAGCAACTGGCATTGGCCAAGAACTGCTGGACATGGCTGATAATCACCCTGTTATCGCGATTAAATCTCTTAAAGTTGGAGCTTTTCAAGGTGTTTCATTGTCTACTATCAGCAGAAGCAATGTGGTGATAAACCCTAATTCTCCAGAAGCTACAAAGTTGAAATCTTGGTATGATGCTGAAGGGAAAGAGACATCTATGTCTGCTATTGGTTCTGGGATGAGTTCTTCTGCCAACAACGGATCAAGATCCATGTATTCTGACAGAGTTTTTCTTTCTCACATCACAAGCAATCCCTCTTTAGGCGAGGAGAAGCCTGTATTCTTCAGCACTAGAGCTTACATAAGCTTCATCAAGCCAGACCAAACAATGTGGTACCGTGCTTGCAAGACCTGTAACAAGAAAGTTACTGAAGCAATGGACTCTGGTTATTGGTGTGAAAGTTGTCAGAAAAAGGACCAAGAATGCAGTTTAAGGTATATAATGGCGGTGAAAGTCTCCGACTCAACTGGTGAAACTTGGTTATCTGCATTCAATGATGAAGCAGAGAAGATTATCGGCTGCACAGCTGATGATCTCAATGATCTAAAATCAGAGGAAGGTGAAGTGAATGAGTTTCAGACAAAACTGAAAGAAGCTACTTGGTCATCTCATCTCTTCCGGATTAGTGTTTCTCAGCAAGAGTATAACAGTGAGAAGAGACAGAGGATAACTGTAAGAGGTGTTTCTCCAATTGACTTTGCTGCTGAAACAAGATTATTGCTCCAAGACATTTCCAAGAACAAGACATCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

604

Amino Acids

67.29

Weight (kDa)

6.15

Isoelectric Point (pI)

35.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 104 4.1e-25 Replication factor-A protein 1, N-terminal domain
DUF223 PF02721 158 - 262 1.1e-10 Domain of unknown function DUF223
tRNA_anti-codon PF01336 170 - 255 5.8e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 287 - 384 2.3e-22 Replication protein A OB domain
Rep_fac-A_C PF08646 447 - 593 2.5e-47 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 290
AarI CACCTGC 1 cut(s) 448
AasI GACNNNNNNGTC 1 cut(s) 1216
Acc36I ACCTGC 1 cut(s) 448
Acc65I GGTACC 1 cut(s) 1392
AccB1I GGYRCC 1 cut(s) 1392
AccI GTMKAC 2 cut(s) 242, 1121
AccII CGCG 2 cut(s) 32, 1076
AccIII TCCGGA 1 cut(s) 1677
AciI CCGC 2 cut(s) 338, 1505
AclWI GGATC 5 cut(s) 108, 709, 943, 1260, 1267
AcoI YGGCCR 1 cut(s) 1033
AcsI RAATTY 2 cut(s) 769, 821
AcuI CTGAAG 3 cut(s) 1218, 1327, 1449
AfaI GTAC 4 cut(s) 686, 834, 853, 1394
AfiI CCNNNNNNNGG 2 cut(s) 500, 1319
AflII CTTAAG 2 cut(s) 104, 707
AjnI CCWGG 2 cut(s) 259, 695
Alw26I GTCTC 5 cut(s) 236, 974, 1202, 1519, 1711
AlwI GGATC 5 cut(s) 108, 709, 943, 1260, 1267
AlwNI CAGNNNCTG 1 cut(s) 217
Aor13HI TCCGGA 1 cut(s) 1677
AoxI GGCC 1 cut(s) 1033
ApeKI GCWGC 6 cut(s) 434, 443, 641, 644, 1578, 1760
ApoI RAATTY 2 cut(s) 769, 821
Asp718I GGTACC 1 cut(s) 1392
AspS9I GGNCC 1 cut(s) 1474
AsuHPI GGTGA 5 cut(s) 1055, 1156, 1519, 1541, 1631
AvaII GGWCC 1 cut(s) 1474
BaeI ACNNNNGTAYC 2 cut(s) 843, 876
BalI TGGCCA 1 cut(s) 1035
BanI GGYRCC 1 cut(s) 1392
BbvI GCAGC 6 cut(s) 421, 430, 631, 653, 1565, 1747
BccI CCATC 3 cut(s) 17, 137, 667
BciT130I CCWGG 2 cut(s) 261, 697
BcoDI GTCTC 5 cut(s) 236, 974, 1202, 1519, 1711
BfaI CTAG 3 cut(s) 789, 1019, 1350
BfrI CTTAAG 2 cut(s) 104, 707
BfuAI ACCTGC 1 cut(s) 448
BisI GCNGC 6 cut(s) 435, 444, 642, 645, 1579, 1761
BlsI GCNGC 6 cut(s) 436, 445, 643, 646, 1580, 1762
Bme1390I CCNGG 2 cut(s) 261, 697
Bme18I GGWCC 1 cut(s) 1474
BmgT120I GGNCC 1 cut(s) 1474
BmiI GGNNCC 1 cut(s) 1394
BmrFI CCNGG 2 cut(s) 261, 697
BmsI GCATC 1 cut(s) 1183
BpmI CTGGAG 1 cut(s) 1146
BpuEI CTTGAG 1 cut(s) 257
Bsa29I ATCGAT 1 cut(s) 121
BsaAI YACGTR 1 cut(s) 859
BsaBI GATNNNNATC 1 cut(s) 1264
BsaJI CCNNGG 1 cut(s) 696
BsaWI WCCGGW 1 cut(s) 1677
Bsc4I CCNNNNNNNGG 2 cut(s) 500, 1319
Bse1I ACTGG 2 cut(s) 1030, 1531
Bse3DI GCAATG 3 cut(s) 171, 1144, 1440
Bse8I GATNNNNATC 1 cut(s) 1264
BseAI TCCGGA 1 cut(s) 1677
BseBI CCWGG 2 cut(s) 261, 697
BseCI ATCGAT 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 696
BseGI GGATG 3 cut(s) 472, 581, 1242
BseJI GATNNNNATC 1 cut(s) 1264
BseLI CCNNNNNNNGG 2 cut(s) 500, 1319
BseMI GCAATG 3 cut(s) 171, 1144, 1440
BseMII CTCAG 4 cut(s) 24, 339, 765, 1706
BseNI ACTGG 2 cut(s) 1030, 1531
BseRI GAGGAG 3 cut(s) 55, 582, 1340
BseXI GCAGC 6 cut(s) 421, 430, 631, 653, 1565, 1747
BsgI GTGCAG 3 cut(s) 300, 420, 1564
Bsh1236I CGCG 2 cut(s) 32, 1076
BshFI GGCC 1 cut(s) 1035
BshNI GGYRCC 1 cut(s) 1392
BshVI ATCGAT 1 cut(s) 121
BsiSI CCGG 1 cut(s) 1678
BslFI GGGAC 1 cut(s) 988
BslI CCNNNNNNNGG 2 cut(s) 500, 1319
BsmAI GTCTC 5 cut(s) 236, 974, 1202, 1519, 1711
BsmBI CGTCTC 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 988
BsmI GAATGC 2 cut(s) 1487, 1547
BsnI GGCC 1 cut(s) 1035
Bsp13I TCCGGA 1 cut(s) 1677
Bsp143I GATC 8 cut(s) 100, 701, 948, 1015, 1259, 1265, 1591, 1600
Bsp68I TCGCGA 2 cut(s) 32, 1076
BspACI CCGC 2 cut(s) 338, 1505
BspANI GGCC 1 cut(s) 1035
BspCNI CTCAG 4 cut(s) 23, 338, 766, 1705
BspDI ATCGAT 1 cut(s) 121
BspEI TCCGGA 1 cut(s) 1677
BspFNI CGCG 2 cut(s) 32, 1076
BspHI TCATGA 1 cut(s) 543
BspLI GGNNCC 1 cut(s) 1394
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 5 cut(s) 108, 709, 943, 1260, 1267
BspT107I GGYRCC 1 cut(s) 1392
BspTI CTTAAG 2 cut(s) 104, 707
BsrDI GCAATG 3 cut(s) 171, 1144, 1440
BsrI ACTGG 2 cut(s) 1030, 1531
BssECI CCNNGG 1 cut(s) 696
BssMI GATC 8 cut(s) 100, 701, 948, 1015, 1259, 1265, 1591, 1600
BssNAI GTATAC 1 cut(s) 243
Bst1107I GTATAC 1 cut(s) 243
Bst2UI CCWGG 2 cut(s) 261, 697
Bst4CI ACNGT 7 cut(s) 37, 247, 736, 994, 1397, 1709, 1732
Bst6I CTCTTC 2 cut(s) 1679, 1709
BstAFI CTTAAG 2 cut(s) 104, 707
BstAPI GCANNNNNTGC 1 cut(s) 443
BstBAI YACGTR 1 cut(s) 859
BstC8I GCNNGC 3 cut(s) 111, 714, 1402
BstDEI CTNAG 5 cut(s) 10, 325, 774, 1692, 1809
BstENI CCTNNNNNAGG 1 cut(s) 498
BstF5I GGATG 3 cut(s) 472, 581, 1242
BstFNI CGCG 2 cut(s) 32, 1076
BstKTI GATC 8 cut(s) 103, 704, 951, 1018, 1262, 1268, 1594, 1603
BstMAI GTCTC 5 cut(s) 236, 974, 1202, 1519, 1711
BstMBI GATC 8 cut(s) 100, 701, 948, 1015, 1259, 1265, 1591, 1600
BstMWI GCNNNNNNNGC 4 cut(s) 398, 440, 443, 452
BstNI CCWGG 2 cut(s) 261, 697
BstSCI CCNGG 2 cut(s) 259, 695
BstUI CGCG 2 cut(s) 32, 1076
BstV1I GCAGC 6 cut(s) 421, 430, 631, 653, 1565, 1747
BstX2I RGATCY 2 cut(s) 100, 1265
BstYI RGATCY 2 cut(s) 100, 1265
BstZ17I GTATAC 1 cut(s) 243
Bsu15I ATCGAT 1 cut(s) 121
BsuRI GGCC 1 cut(s) 1035
BsuTUI ATCGAT 1 cut(s) 121
BtsCI GGATG 3 cut(s) 472, 581, 1242
BtsIMutI CAGTG 2 cut(s) 18, 1714
BtuMI TCGCGA 2 cut(s) 32, 1076
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 3 cut(s) 111, 714, 1402
CaiI CAGNNNCTG 1 cut(s) 217
CciI TCATGA 1 cut(s) 543
Cfr13I GGNCC 1 cut(s) 1474
ClaI ATCGAT 1 cut(s) 121
Csp6I GTAC 4 cut(s) 685, 833, 852, 1393
CspCI CAANNNNNGTGG 2 cut(s) 1370, 1405
CviAII CATG 5 cut(s) 380, 544, 916, 1051, 1270
CviQI GTAC 4 cut(s) 685, 833, 852, 1393
DdeI CTNAG 5 cut(s) 10, 325, 774, 1692, 1809
DpnI GATC 8 cut(s) 102, 703, 950, 1017, 1261, 1267, 1593, 1602
DpnII GATC 8 cut(s) 100, 701, 948, 1015, 1259, 1265, 1591, 1600
DrdI GACNNNNNNGTC 1 cut(s) 1216
DseDI GACNNNNNNGTC 1 cut(s) 1216
EaeI YGGCCR 1 cut(s) 1033
Eam1104I CTCTTC 2 cut(s) 1679, 1709
EarI CTCTTC 2 cut(s) 1679, 1709
EciI GGCGGA 1 cut(s) 353
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 1474
Eco57I CTGAAG 3 cut(s) 1218, 1327, 1449
EcoNI CCTNNNNNAGG 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 2 cut(s) 259, 695
EcoRV GATATC 1 cut(s) 193
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 5 cut(s) 383, 547, 919, 1054, 1273
FaqI GGGAC 1 cut(s) 988
FatI CATG 5 cut(s) 379, 543, 915, 1050, 1269
FblI GTMKAC 2 cut(s) 242, 1121
Fnu4HI GCNGC 6 cut(s) 435, 444, 642, 645, 1579, 1761
FokI GGATG 3 cut(s) 459, 588, 1249
Fsp4HI GCNGC 6 cut(s) 435, 444, 642, 645, 1579, 1761
FspBI CTAG 3 cut(s) 789, 1019, 1350
GluI GCNGC 6 cut(s) 435, 444, 642, 645, 1579, 1761
GsuI CTGGAG 1 cut(s) 1146
HaeIII GGCC 1 cut(s) 1035
HapII CCGG 1 cut(s) 1678
Hin1II CATG 5 cut(s) 383, 547, 919, 1054, 1273
HindIII AAGCTT 3 cut(s) 348, 710, 1363
HpaII CCGG 1 cut(s) 1678
HphI GGTGA 5 cut(s) 1055, 1156, 1519, 1541, 1631
Hpy166II GTNNAC 4 cut(s) 243, 685, 807, 1122
Hpy188III TCNNGA 9 cut(s) 31, 201, 544, 670, 730, 1075, 1163, 1263, 1678
Hpy8I GTNNAC 4 cut(s) 243, 685, 807, 1122
HpyAV CCTTC 6 cut(s) 459, 566, 602, 921, 1193, 1610
HpyCH4III ACNGT 7 cut(s) 37, 247, 736, 994, 1397, 1709, 1732
HpyCH4IV ACGT 2 cut(s) 128, 858
HpyCH4V TGCA 8 cut(s) 317, 437, 446, 647, 1404, 1485, 1547, 1581
HpyF10VI GCNNNNNNNGC 4 cut(s) 398, 440, 443, 452
HpyF3I CTNAG 5 cut(s) 10, 325, 774, 1692, 1809
HpySE526I ACGT 2 cut(s) 128, 858
Hsp92II CATG 5 cut(s) 383, 547, 919, 1054, 1273
Kpn2I TCCGGA 1 cut(s) 1677
KpnI GGTACC 1 cut(s) 1396
Kzo9I GATC 8 cut(s) 100, 701, 948, 1015, 1259, 1265, 1591, 1600
LmnI GCTCC 2 cut(s) 1097, 1785
Lsp1109I GCAGC 6 cut(s) 421, 430, 631, 653, 1565, 1747
LweI GCATC 1 cut(s) 1183
MaeI CTAG 3 cut(s) 789, 1019, 1350
MaeII ACGT 2 cut(s) 128, 858
MaeIII GTNAC 5 cut(s) 13, 168, 526, 1412, 1423
MalI GATC 8 cut(s) 102, 703, 950, 1017, 1261, 1267, 1593, 1602
MboI GATC 8 cut(s) 100, 701, 948, 1015, 1259, 1265, 1591, 1600
MfeI CAATTG 1 cut(s) 1749
MflI RGATCY 2 cut(s) 100, 1265
MlsI TGGCCA 1 cut(s) 1035
MluCI AATT 5 cut(s) 81, 769, 821, 1156, 1749
MluNI TGGCCA 1 cut(s) 1035
MlyI GAGTC 4 cut(s) 317, 549, 1433, 1514
MmeI TCCRAC 3 cut(s) 488, 1075, 1542
Mox20I TGGCCA 1 cut(s) 1035
MroI TCCGGA 1 cut(s) 1677
MscI TGGCCA 1 cut(s) 1035
Msp20I TGGCCA 1 cut(s) 1035
MspA1I CMGCKG 2 cut(s) 644, 1586
MspCI CTTAAG 2 cut(s) 104, 707
MspI CCGG 1 cut(s) 1678
MspR9I CCNGG 2 cut(s) 261, 697
MunI CAATTG 1 cut(s) 1749
Mva1269I GAATGC 2 cut(s) 1487, 1547
MvaI CCWGG 2 cut(s) 261, 697
MvnI CGCG 2 cut(s) 32, 1076
MwoI GCNNNNNNNGC 4 cut(s) 398, 440, 443, 452
NdeII GATC 8 cut(s) 100, 701, 948, 1015, 1259, 1265, 1591, 1600
NlaIII CATG 5 cut(s) 383, 547, 919, 1054, 1273
NlaIV GGNNCC 1 cut(s) 1394
NmuCI GTSAC 2 cut(s) 13, 526
NruI TCGCGA 2 cut(s) 32, 1076
PagI TCATGA 1 cut(s) 543
PaqCI CACCTGC 1 cut(s) 448
PctI GAATGC 2 cut(s) 1487, 1547
PfeI GAWTC 6 cut(s) 59, 208, 214, 619, 666, 862
PkrI GCNGC 6 cut(s) 436, 445, 643, 646, 1580, 1762
PleI GAGTC 4 cut(s) 317, 548, 1433, 1514
PpsI GAGTC 4 cut(s) 317, 548, 1433, 1514
Ppu21I YACGTR 1 cut(s) 859
PsiI TTATAA 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 259, 695
PspGI CCWGG 2 cut(s) 259, 695
PspN4I GGNNCC 1 cut(s) 1394
PspPI GGNCC 1 cut(s) 1474
PstNI CAGNNNCTG 1 cut(s) 217
PsuI RGATCY 2 cut(s) 100, 1265
PvuII CAGCTG 2 cut(s) 644, 1586
RruI TCGCGA 2 cut(s) 32, 1076
RsaI GTAC 4 cut(s) 686, 834, 853, 1394
RsaNI GTAC 4 cut(s) 685, 833, 852, 1393
SatI GCNGC 6 cut(s) 435, 444, 642, 645, 1579, 1761
Sau3AI GATC 8 cut(s) 100, 701, 948, 1015, 1259, 1265, 1591, 1600
Sau96I GGNCC 1 cut(s) 1474
SchI GAGTC 4 cut(s) 317, 549, 1433, 1514
ScrFI CCNGG 2 cut(s) 261, 697
SfaNI GCATC 1 cut(s) 1183
SinI GGWCC 1 cut(s) 1474
SmlI CTYRAG 3 cut(s) 104, 236, 707
SmoI CTYRAG 3 cut(s) 104, 236, 707
Sse9I AATT 5 cut(s) 81, 769, 821, 1156, 1749
SsiI CCGC 2 cut(s) 338, 1505
SspMI CTAG 3 cut(s) 789, 1019, 1350
StyD4I CCNGG 2 cut(s) 259, 695
TaaI ACNGT 7 cut(s) 37, 247, 736, 994, 1397, 1709, 1732
TaiI ACGT 2 cut(s) 131, 861
TaqI TCGA 2 cut(s) 121, 669
TasI AATT 5 cut(s) 81, 769, 821, 1156, 1749
TatI WGTACW 1 cut(s) 684
TfiI GAWTC 6 cut(s) 59, 208, 214, 619, 666, 862
TscAI CASTG 2 cut(s) 18, 1714
TseFI GTSAC 2 cut(s) 13, 526
TseI GCWGC 6 cut(s) 434, 443, 641, 644, 1578, 1760
Tsp45I GTSAC 2 cut(s) 13, 526
TspDTI ATGAA 6 cut(s) 167, 461, 810, 1104, 1357, 1572
TspGWI ACGGA 1 cut(s) 1272
TspRI CASTG 2 cut(s) 18, 1714
Vha464I CTTAAG 2 cut(s) 104, 707
VpaK11BI GGWCC 1 cut(s) 1474
XagI CCTNNNNNAGG 1 cut(s) 498
XapI RAATTY 2 cut(s) 769, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 844
XmiI GTMKAC 2 cut(s) 242, 1121
XspI CTAG 3 cut(s) 789, 1019, 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.