MD06G1225500.v1.1

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009976
Physical Location & Seq
Forward (+)
35616320 .. 35619626
3307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1225500.v1.1.491

Sequence Viewer

Length: 1908 bp
ATGGCGAAGTTAGGGCCAACCCCAGATGCGATTTCGATCATCTTGGCGAACAAGTCGCCGGATGAGTCCGTTGAGCTGCCGGAGATCGTGGTTCAGGTCTGCGATCTCACCCCCAGGGGCAAGTCTTTCATGTTTACTGCTACTGATGGAACTGCGAAGCTAAAGGGGATGCTCTCCTCGCGGCTGGCTCCCCAGGTCACCTCTGGGGAGATTCAGAATCTGGGTCTCATTCGCGTTTTGGGTTACTCTGTTAATTTCAATTCGAACTTGTCCGAAAACTATCTGCTTGTAACAAAATGTGAGGTGGTTTCGCCTGCGCTTGAGGCGGAGGTGAAAAATGAGGTCAAAAGGGAAGATGTTGGGATCATATTGAATCCCAAGCAAGAAATGGTTTCAGAATCTGCTGCCCAAATTGTGCATGAGCAGGCTGGGAAGGTCAAAAAGGAAGATGTTGGGGTTATATTGAAGCCCAAGCAAGAAATGGTTTCAAAATCTGCTGCCCAAATTGTGCATGAGCAGGCTGGGAATATGGCCCCATCTGCGAGAATGGCTATGACACGAAGGGTTCACCCGCTTGTTTCCTTGAACCCTTACCAAGGAAATTGGACCATAAAGGTTCGAGTTACAAACAAGGGTACCATGCGTACATACAAGAATGCTAGAGGTGAAGGTTGCGTCTTCAATGTGGAGTTAACAGATCAAGATGGCACCCAAATTCAAGCAACAATGTTCAATGAAGCTGCAAAGAAGTTCTTTGAGAAGTTTGAGTTAGGGAAGGTTTATTACATTTCAAAGGGATCTCTGAGGGTTGCTAACAAGCAGTTTAAGACGGTGCAAAATGATTATGAAATGACTTTGAATGAGAATTCTGAGGTGGAAGAAGCTAGCAATGAAGCAGCTTTTGTTCCTGAAGTAAAATTCAATTTTGTGCCCATTGATATGTTGGGTCCTTTTGTTAATGGAAAGGAGCTTGTGGATGTTATTGGGGTTGTTCAAAATGTGTCTCCTACAATGAGCATTAGAAGGAAGAGCAACAATGAGAGTATTCCAAAGCGTGATATCACCATTGCTGATGAAACGAAGAAGACAGTTGTGGTTTCTTTATGGGGTGAGCTCGCAACTGGTGTAGGCCAGGAATTGCTTGACATTGCTGATCAATCTCCCGTAGTTGCTATCAAGTCCCTCAAAGTTGGAGACTTCCAAGGTGTATCGTTGTCAACATTGAGCAGGAGCACAATATTGGTTAATCCAGAGTTACCTGAAGCACAGAAGTTGAGGTCTTGGTATGATTCTGAAGGTAAAGAATCTTCTTTGGCCTCTATTGGCTCTGGTATGAGCCCTTCAGCCAAGAATGGAGGAAGGTCCATGTACTCTGACAGAGTCACCCTGTCTCATATATCTGAAGACCCGTCCTTGGGTGAGGATAAGCCTGTGTTTTTCAGTGTTAAAGCGTTCATAAGTTCGATCAGGCCTGATCAGACAATGTGGTACCGTGCTTGCAAAACTTGTAACAAGAAAGTGACTGAAGCTATTGGGTCTGGTTATTGGTGTGAAGGGTGCCAGAAAAACGATGAAGAATGCAGTTTACGGTACATATTGGTTGTGAGAGTTACTGATGTAAGTGGGGAAACGTATCTTTCTCTTTTCAATGACGAAGCTGAGAGGGTCCTCGGGTGCTCTGCAGATGAACTGGACAAATTGAAATCACAGGACGGAGACGGGAATGAATTCCAACAAAAACTGAAAGAAGCAACTTGGGTTCCTCATCTTTTTAGGGTCAGTGTTACTCTGAATGAGTACAATAACGAGAAGCGGAAGAGGATAACTGCCAGGGCAGTTGCTCCAGTTGATTTTGCTGCTGAATCGAGGTTTTTGCTTGAAGACATATCGAAGATGAAGGCGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

636

Amino Acids

70.13

Weight (kDa)

5.89

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 7 - 104 1.2e-14 Replication factor-A protein 1, N-terminal domain
DUF223 PF02721 190 - 294 4.2e-10 Domain of unknown function DUF223
tRNA_anti-codon PF01336 202 - 287 2e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 322 - 416 5.5e-24 Replication protein A OB domain
Rep_fac-A_C PF08646 479 - 625 3.2e-45 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 635, 1488
AccB1I GGYRCC 4 cut(s) 635, 707, 1488, 1557
AccII CGCG 2 cut(s) 181, 234
AciI CCGC 4 cut(s) 181, 326, 572, 1813
AclWI GGATC 2 cut(s) 371, 805
AcsI RAATTY 4 cut(s) 714, 865, 917, 1727
AcuI CTGAAG 6 cut(s) 930, 1281, 1314, 1326, 1422, 1545
AcyI GRCGYC 1 cut(s) 1901
AfaI GTAC 6 cut(s) 637, 646, 1370, 1490, 1592, 1799
AfiI CCNNNNNNNGG 3 cut(s) 596, 1414, 1415
AjnI CCWGG 4 cut(s) 113, 192, 1131, 1829
AluBI AGCT 9 cut(s) 76, 160, 740, 884, 899, 970, 1114, 1529, 1658
AluI AGCT 9 cut(s) 76, 160, 740, 884, 899, 970, 1114, 1529, 1658
Alw21I GWGCWC 3 cut(s) 1116, 1235, 1679
Alw26I GTCTC 5 cut(s) 230, 1008, 1188, 1395, 1710
AlwI GGATC 2 cut(s) 371, 805
AlwNI CAGNNNCTG 2 cut(s) 220, 401
Ama87I CYCGRG 1 cut(s) 1670
AoxI GGCC 5 cut(s) 14, 531, 1129, 1314, 1469
ApeKI GCWGC 6 cut(s) 76, 404, 497, 740, 896, 1856
ApoI RAATTY 4 cut(s) 714, 865, 917, 1727
Asp700I GAANNNNTTC 1 cut(s) 1727
Asp718I GGTACC 2 cut(s) 635, 1488
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 6 cut(s) 14, 532, 606, 947, 1362, 1666
AsuHPI GGTGA 9 cut(s) 100, 190, 343, 560, 677, 1054, 1121, 1375, 1430
AsuII TTCGAA 1 cut(s) 263
AsuNHI GCTAGC 1 cut(s) 884
AvaI CYCGRG 1 cut(s) 1670
AvaII GGWCC 4 cut(s) 606, 947, 1362, 1666
BaeGI GKGCMC 1 cut(s) 933
BaeI ACNNNNGTAYC 2 cut(s) 619, 652
BanI GGYRCC 4 cut(s) 635, 707, 1488, 1557
BanII GRGCYC 2 cut(s) 1116, 1340
BarI GAAGNNNNNNTAC 6 cut(s) 767, 799, 1291, 1323, 1324, 1356
BbsI GAAGAC 4 cut(s) 670, 1091, 1410, 1887
Bbv12I GWGCWC 3 cut(s) 1116, 1235, 1679
BbvI GCAGC 6 cut(s) 63, 391, 484, 727, 908, 1843
BccI CCATC 3 cut(s) 140, 544, 698
BcgI CGANNNNNNTGC 2 cut(s) 1855, 1889
BciT130I CCWGG 4 cut(s) 115, 194, 1133, 1831
BclI TGATCA 2 cut(s) 1153, 1474
BcoDI GTCTC 5 cut(s) 230, 1008, 1188, 1395, 1710
BfaI CTAG 2 cut(s) 660, 885
BfmI CTRYAG 1 cut(s) 1680
BisI GCNGC 7 cut(s) 77, 182, 405, 498, 741, 897, 1857
BlsI GCNGC 7 cut(s) 78, 183, 406, 499, 742, 898, 1858
Bme1390I CCNGG 4 cut(s) 115, 194, 1133, 1831
Bme18I GGWCC 4 cut(s) 606, 947, 1362, 1666
BmeT110I CYCGRG 1 cut(s) 1670
BmgT120I GGNCC 6 cut(s) 14, 532, 606, 947, 1362, 1666
BmiI GGNNCC 9 cut(s) 189, 534, 637, 709, 948, 1490, 1559, 1667, 1761
BmrFI CCNGG 4 cut(s) 115, 194, 1133, 1831
BmsI GCATC 2 cut(s) 16, 159
BmtI GCTAGC 1 cut(s) 888
BpiI GAAGAC 4 cut(s) 670, 1091, 1410, 1887
BpmI CTGGAG 1 cut(s) 1827
Bpu14I TTCGAA 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 1901
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 8 cut(s) 113, 114, 192, 595, 1201, 1413, 1669, 1830
Bsc4I CCNNNNNNNGG 3 cut(s) 596, 1414, 1415
Bse1I ACTGG 3 cut(s) 1126, 1695, 1844
Bse3DI GCAATG 3 cut(s) 895, 1065, 1146
Bse8I GATNNNNATC 1 cut(s) 35
BseBI CCWGG 4 cut(s) 115, 194, 1133, 1831
BseDI CCNNGG 8 cut(s) 113, 114, 192, 595, 1201, 1413, 1669, 1830
BseGI GGATG 3 cut(s) 67, 174, 982
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 3 cut(s) 596, 1414, 1415
BseMI GCAATG 3 cut(s) 895, 1065, 1146
BseMII CTCAG 3 cut(s) 794, 861, 1650
BseNI ACTGG 3 cut(s) 1126, 1695, 1844
BseRI GAGGAG 1 cut(s) 166
BseSI GKGCMC 1 cut(s) 933
BseXI GCAGC 6 cut(s) 63, 391, 484, 727, 908, 1843
BseYI CCCAGC 2 cut(s) 428, 521
Bsh1236I CGCG 2 cut(s) 181, 234
BshFI GGCC 5 cut(s) 16, 533, 1131, 1316, 1471
BshNI GGYRCC 4 cut(s) 635, 707, 1488, 1557
BsiHKAI GWGCWC 3 cut(s) 1116, 1235, 1679
BsiHKCI CYCGRG 1 cut(s) 1670
BsiSI CCGG 2 cut(s) 59, 80
BslFI GGGAC 1 cut(s) 1165
BslI CCNNNNNNNGG 3 cut(s) 596, 1414, 1415
BsmAI GTCTC 5 cut(s) 230, 1008, 1188, 1395, 1710
BsmBI CGTCTC 1 cut(s) 1710
BsmFI GGGAC 1 cut(s) 1165
BsmI GAATGC 2 cut(s) 661, 1583
BsnI GGCC 5 cut(s) 16, 533, 1131, 1316, 1471
Bso31I GGTCTC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 1670
Bsp119I TTCGAA 1 cut(s) 263
Bsp1286I GDGCHC 5 cut(s) 933, 1116, 1235, 1340, 1679
Bsp143I GATC 9 cut(s) 36, 84, 103, 363, 697, 797, 1153, 1464, 1474
BspACI CCGC 4 cut(s) 181, 326, 572, 1813
BspANI GGCC 5 cut(s) 16, 533, 1131, 1316, 1471
BspCNI CTCAG 3 cut(s) 795, 862, 1651
BspFNI CGCG 2 cut(s) 181, 234
BspLI GGNNCC 9 cut(s) 189, 534, 637, 709, 948, 1490, 1559, 1667, 1761
BspMAI CTGCAG 1 cut(s) 1684
BspOI GCTAGC 1 cut(s) 888
BspPI GGATC 2 cut(s) 371, 805
BspQI GCTCTTC 1 cut(s) 1022
BspT104I TTCGAA 1 cut(s) 263
BspT107I GGYRCC 4 cut(s) 635, 707, 1488, 1557
BspTNI GGTCTC 1 cut(s) 230
BsrDI GCAATG 3 cut(s) 895, 1065, 1146
BsrI ACTGG 3 cut(s) 1126, 1695, 1844
BssECI CCNNGG 8 cut(s) 113, 114, 192, 595, 1201, 1413, 1669, 1830
BssMI GATC 9 cut(s) 36, 84, 103, 363, 697, 797, 1153, 1464, 1474
BssNI GRCGYC 1 cut(s) 1901
BssT1I CCWWGG 3 cut(s) 595, 1201, 1413
Bst2UI CCWGG 4 cut(s) 115, 194, 1133, 1831
Bst4CI ACNGT 4 cut(s) 832, 1090, 1493, 1590
Bst6I CTCTTC 2 cut(s) 1022, 1811
BstACI GRCGYC 1 cut(s) 1901
BstBI TTCGAA 1 cut(s) 263
BstC8I GCNNGC 7 cut(s) 186, 315, 426, 519, 886, 1116, 1498
BstDEI CTNAG 3 cut(s) 803, 870, 1659
BstEII GGTNACC 1 cut(s) 196
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 3 cut(s) 67, 174, 982
BstFNI CGCG 2 cut(s) 181, 234
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 9 cut(s) 39, 87, 106, 366, 700, 800, 1156, 1467, 1477
BstMAI GTCTC 5 cut(s) 230, 1008, 1188, 1395, 1710
BstMBI GATC 9 cut(s) 36, 84, 103, 363, 697, 797, 1153, 1464, 1474
BstMWI GCNNNNNNNGC 4 cut(s) 178, 323, 539, 548
BstNI CCWGG 4 cut(s) 115, 194, 1133, 1831
BstPI GGTNACC 1 cut(s) 196
BstSCI CCNGG 4 cut(s) 113, 192, 1131, 1829
BstSFI CTRYAG 1 cut(s) 1680
BstSLI GKGCMC 1 cut(s) 933
BstUI CGCG 2 cut(s) 181, 234
BstV1I GCAGC 6 cut(s) 63, 391, 484, 727, 908, 1843
BstV2I GAAGAC 4 cut(s) 670, 1091, 1410, 1887
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
BsuRI GGCC 5 cut(s) 16, 533, 1131, 1316, 1471
BtsCI GGATG 3 cut(s) 67, 174, 982
BtsIMutI CAGTG 2 cut(s) 1447, 1786
Cac8I GCNNGC 7 cut(s) 186, 315, 426, 519, 886, 1116, 1498
CaiI CAGNNNCTG 2 cut(s) 220, 401
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 6 cut(s) 14, 532, 606, 947, 1362, 1666
CseI GACGC 2 cut(s) 664, 1890
Csp6I GTAC 6 cut(s) 636, 645, 1369, 1489, 1591, 1798
CviAII CATG 5 cut(s) 130, 419, 512, 640, 1366
CviQI GTAC 6 cut(s) 636, 645, 1369, 1489, 1591, 1798
DdeI CTNAG 3 cut(s) 803, 870, 1659
DpnI GATC 9 cut(s) 38, 86, 105, 365, 699, 799, 1155, 1466, 1476
DpnII GATC 9 cut(s) 36, 84, 103, 363, 697, 797, 1153, 1464, 1474
Eam1104I CTCTTC 2 cut(s) 1022, 1811
EarI CTCTTC 2 cut(s) 1022, 1811
EciI GGCGGA 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 1114
Eco130I CCWWGG 3 cut(s) 595, 1201, 1413
Eco147I AGGCCT 1 cut(s) 1471
Eco24I GRGCYC 2 cut(s) 1116, 1340
Eco31I GGTCTC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 1060
Eco47I GGWCC 4 cut(s) 606, 947, 1362, 1666
Eco53kI GAGCTC 1 cut(s) 1114
Eco57I CTGAAG 6 cut(s) 930, 1281, 1314, 1326, 1422, 1545
Eco88I CYCGRG 1 cut(s) 1670
Eco91I GGTNACC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 1114
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoO109I RGGNCCY 2 cut(s) 947, 1666
EcoO65I GGTNACC 1 cut(s) 196
EcoRI GAATTC 2 cut(s) 865, 1727
EcoRII CCWGG 4 cut(s) 113, 192, 1131, 1829
EcoRV GATATC 1 cut(s) 1060
EcoT14I CCWWGG 3 cut(s) 595, 1201, 1413
EcoT38I GRGCYC 2 cut(s) 1116, 1340
ErhI CCWWGG 3 cut(s) 595, 1201, 1413
Esp3I CGTCTC 1 cut(s) 1710
FaeI CATG 5 cut(s) 133, 422, 515, 643, 1369
FalI AAGNNNNNCTT 2 cut(s) 737, 769
FaqI GGGAC 1 cut(s) 1165
FatI CATG 5 cut(s) 129, 418, 511, 639, 1365
FauI CCCGC 1 cut(s) 579
FbaI TGATCA 2 cut(s) 1153, 1474
Fnu4HI GCNGC 7 cut(s) 77, 182, 405, 498, 741, 897, 1857
FokI GGATG 3 cut(s) 74, 181, 989
FriOI GRGCYC 2 cut(s) 1116, 1340
Fsp4HI GCNGC 7 cut(s) 77, 182, 405, 498, 741, 897, 1857
FspBI CTAG 2 cut(s) 660, 885
GlaI GCGC 1 cut(s) 318
GluI GCNGC 7 cut(s) 77, 182, 405, 498, 741, 897, 1857
GsaI CCCAGC 2 cut(s) 432, 525
GsuI CTGGAG 1 cut(s) 1827
HaeIII GGCC 5 cut(s) 16, 533, 1131, 1316, 1471
HapII CCGG 2 cut(s) 59, 80
HgaI GACGC 2 cut(s) 664, 1890
HhaI GCGC 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 1901
Hin1II CATG 5 cut(s) 133, 422, 515, 643, 1369
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HincII GTYRAC 2 cut(s) 693, 1218
HindII GTYRAC 2 cut(s) 693, 1218
HinfI GANTC 9 cut(s) 65, 211, 217, 373, 398, 1289, 1304, 1380, 1862
HpaI GTTAAC 1 cut(s) 693
HpaII CCGG 2 cut(s) 59, 80
HphI GGTGA 9 cut(s) 100, 190, 343, 560, 677, 1054, 1121, 1375, 1430
Hpy166II GTNNAC 5 cut(s) 135, 568, 693, 1218, 1586
Hpy188III TCNNGA 3 cut(s) 701, 908, 1250
Hpy8I GTNNAC 5 cut(s) 135, 568, 693, 1218, 1586
HpyCH4III ACNGT 4 cut(s) 832, 1090, 1493, 1590
HpyCH4IV ACGT 1 cut(s) 1631
HpyCH4V TGCA 7 cut(s) 418, 511, 743, 835, 1500, 1581, 1682
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 323, 539, 548
HpyF3I CTNAG 3 cut(s) 803, 870, 1659
HpySE526I ACGT 1 cut(s) 1631
Hsp92I GRCGYC 1 cut(s) 1901
Hsp92II CATG 5 cut(s) 133, 422, 515, 643, 1369
HspAI GCGC 1 cut(s) 317
KpnI GGTACC 2 cut(s) 639, 1492
Ksp22I TGATCA 2 cut(s) 1153, 1474
KspAI GTTAAC 1 cut(s) 693
Kzo9I GATC 9 cut(s) 36, 84, 103, 363, 697, 797, 1153, 1464, 1474
LguI GCTCTTC 1 cut(s) 1022
LmnI GCTCC 4 cut(s) 193, 967, 1230, 1846
Lsp1109I GCAGC 6 cut(s) 63, 391, 484, 727, 908, 1843
LweI GCATC 2 cut(s) 16, 159
MaeI CTAG 2 cut(s) 660, 885
MaeII ACGT 1 cut(s) 1631
MalI GATC 9 cut(s) 38, 86, 105, 365, 699, 799, 1155, 1466, 1476
MboI GATC 9 cut(s) 36, 84, 103, 363, 697, 797, 1153, 1464, 1474
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 5 cut(s) 933, 1116, 1235, 1340, 1679
MlyI GAGTC 2 cut(s) 74, 1389
MmeI TCCRAC 2 cut(s) 1171, 1756
MroXI GAANNNNTTC 1 cut(s) 1727
MseI TTAA 6 cut(s) 252, 692, 825, 957, 1245, 1446
MslI CAYNNNNRTG 1 cut(s) 938
MspI CCGG 2 cut(s) 59, 80
MspR9I CCNGG 4 cut(s) 115, 194, 1133, 1831
Mva1269I GAATGC 2 cut(s) 661, 1583
MvaI CCWGG 4 cut(s) 115, 194, 1133, 1831
MvnI CGCG 2 cut(s) 181, 234
MwoI GCNNNNNNNGC 4 cut(s) 178, 323, 539, 548
NdeII GATC 9 cut(s) 36, 84, 103, 363, 697, 797, 1153, 1464, 1474
NheI GCTAGC 1 cut(s) 884
NlaIII CATG 5 cut(s) 133, 422, 515, 643, 1369
NlaIV GGNNCC 9 cut(s) 189, 534, 637, 709, 948, 1490, 1559, 1667, 1761
NmuCI GTSAC 3 cut(s) 196, 1381, 1519
NspV TTCGAA 1 cut(s) 263
PasI CCCWGGG 1 cut(s) 114
PceI AGGCCT 1 cut(s) 1471
PciSI GCTCTTC 1 cut(s) 1022
PctI GAATGC 2 cut(s) 661, 1583
PdmI GAANNNNTTC 1 cut(s) 1727
PfeI GAWTC 7 cut(s) 211, 217, 373, 398, 1289, 1304, 1862
PflFI GACNNNGTC 1 cut(s) 1379
PkrI GCNGC 7 cut(s) 78, 183, 406, 499, 742, 898, 1858
PleI GAGTC 2 cut(s) 73, 1388
PpsI GAGTC 2 cut(s) 73, 1388
PpuMI RGGWCCY 2 cut(s) 947, 1666
Psp124BI GAGCTC 1 cut(s) 1116
Psp5II RGGWCCY 2 cut(s) 947, 1666
Psp6I CCWGG 4 cut(s) 113, 192, 1131, 1829
PspEI GGTNACC 1 cut(s) 196
PspFI CCCAGC 2 cut(s) 428, 521
PspGI CCWGG 4 cut(s) 113, 192, 1131, 1829
PspN4I GGNNCC 9 cut(s) 189, 534, 637, 709, 948, 1490, 1559, 1667, 1761
PspPI GGNCC 6 cut(s) 14, 532, 606, 947, 1362, 1666
PspPPI RGGWCCY 2 cut(s) 947, 1666
PstI CTGCAG 1 cut(s) 1684
PstNI CAGNNNCTG 2 cut(s) 220, 401
PsuI RGATCY 1 cut(s) 797
PsyI GACNNNGTC 1 cut(s) 1379
RsaI GTAC 6 cut(s) 637, 646, 1370, 1490, 1592, 1799
RsaNI GTAC 6 cut(s) 636, 645, 1369, 1489, 1591, 1798
RseI CAYNNNNRTG 1 cut(s) 938
SacI GAGCTC 1 cut(s) 1116
SapI GCTCTTC 1 cut(s) 1022
SaqAI TTAA 6 cut(s) 252, 692, 825, 957, 1245, 1446
SatI GCNGC 7 cut(s) 77, 182, 405, 498, 741, 897, 1857
Sau3AI GATC 9 cut(s) 36, 84, 103, 363, 697, 797, 1153, 1464, 1474
Sau96I GGNCC 6 cut(s) 14, 532, 606, 947, 1362, 1666
SchI GAGTC 2 cut(s) 74, 1389
ScrFI CCNGG 4 cut(s) 115, 194, 1133, 1831
SduI GDGCHC 5 cut(s) 933, 1116, 1235, 1340, 1679
SfaNI GCATC 2 cut(s) 16, 159
SfcI CTRYAG 1 cut(s) 1680
SfuI TTCGAA 1 cut(s) 263
SinI GGWCC 4 cut(s) 606, 947, 1362, 1666
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SseBI AGGCCT 1 cut(s) 1471
SsiI CCGC 4 cut(s) 181, 326, 572, 1813
SspI AATATT 1 cut(s) 1239
SspMI CTAG 2 cut(s) 660, 885
SstI GAGCTC 1 cut(s) 1116
StuI AGGCCT 1 cut(s) 1471
StyD4I CCNGG 4 cut(s) 113, 192, 1131, 1829
StyI CCWWGG 3 cut(s) 595, 1201, 1413
TaaI ACNGT 4 cut(s) 832, 1090, 1493, 1590
TaiI ACGT 1 cut(s) 1634
TaqI TCGA 6 cut(s) 35, 263, 619, 1463, 1865, 1889
TatI WGTACW 2 cut(s) 1368, 1797
TauI GCSGC 1 cut(s) 184
TfiI GAWTC 7 cut(s) 211, 217, 373, 398, 1289, 1304, 1862
Tru1I TTAA 6 cut(s) 252, 692, 825, 957, 1245, 1446
Tru9I TTAA 6 cut(s) 252, 692, 825, 957, 1245, 1446
TscAI CASTG 2 cut(s) 1447, 1786
TseFI GTSAC 3 cut(s) 196, 1381, 1519
TseI GCWGC 6 cut(s) 76, 404, 497, 740, 896, 1856
Tsp45I GTSAC 3 cut(s) 196, 1381, 1519
TspDTI ATGAA 9 cut(s) 118, 750, 861, 906, 1089, 1444, 1587, 1701, 1740
TspGWI ACGGA 2 cut(s) 58, 1728
TspRI CASTG 2 cut(s) 1447, 1786
Tth111I GACNNNGTC 1 cut(s) 1379
VpaK11BI GGWCC 4 cut(s) 606, 947, 1362, 1666
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 4 cut(s) 714, 865, 917, 1727
XcmI CCANNNNNNNNNTGG 4 cut(s) 200, 385, 478, 940
XmnI GAANNNNTTC 1 cut(s) 1727
XspI CTAG 2 cut(s) 660, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.