Rh7DG056400

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009976
Physical Location & Seq
Forward (+)
4329982 .. 4333133
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG056400.1

Sequence Viewer

Length: 1719 bp
ATGGCGAAGCTTGGACCAACCCCAGATGCGATTTCGACCCTCTTGGCCAACCCATCGCCGAAAGAGTCCAGCCCGATCCCGGAGATCGTCGTCCAGGTCGTCGACCTCAATCTTAAGGGCATCAACCGTTATATGTTTACTGCTAGTGATGGAAAGATGAGGCTAAAGACGATATATATGATTGTAACAAAATGTGAGATGGTCTCACCTGCGCTTGAGATGGAGATTAAAAGTGAGGTCAGTAAAGATGAGGTGAAAGGAGAACAGGTTGGGATTATTTTGAAGCCCAAGCAAGAGATGGTGACTAAATCAGCTGCTCAAATTATGCATGAGCAGAATGCAAATATGGCCCCGGCTGCACGAATGGCCATGACCAGAAGGGTTCATCCTATTGTTTCCTTGAATCCTTATCAAGGCAATTGGACCATAAAGGTTCGTGTTACAAACAAGGGAACCATGCGTACTTACTCGAATGCTAGAGGTCAAGGCTGTGTCTTCAATGTGGAGTTAACAGATGAAGATGGAACTGAAATTCGAGCTACAATGTTTAATGAAGCTGCCAAGAAGTTCTATGAAAAGTTTCAACTAGGGAAGGTCTATTATGTTTCAAAGGGAGCTCTAAAGGTTGCTAACAAGAAGTTTAGCACAGTACAAAATGATTATGAAATGACCTTAAATGAGAATTCTGAGGTGGATGAAGTCAGCAATGAAGAAGCTTTTGTTCCTGAAACAAAATTTGATTTTATCCCAATTGATATGTTGGGTCCCCATGTTAACAGCAAGGTGCTTGTGGATGTTATTGGAGTTGTTACAAATGTGTCTCCTACAATGAGCATCAGAAGAAAGAGCGACAATGAGAATATCCCAAAGCGTGACATTACCATTGCCGATGAGTCGAAAAAAGTGGTTGTGGTTTCTTTATGGAATGATCTTGCAACTGGTGTTGGCCAGGAATTGCTGGACATTGCTGATCAATCTCCTGTAGTTGCTATCAAATCCCTTAAAGTTGGAGACTATCAAGGTGTATCCTTGTCTACGTTGTCCAGGAGCTCAATATTTGTAAATCCAGATTTACCTGAAGCAAAGAGGTTGAAATCCTGGTATGATTCTGAAGGTAAAGAGGTTTCGATGGCATCTGTTGGCTCTGGTATGAGCCCATCAGCCAATAGTGGAGCATGGTCCATGTACTCTGATAGAATCTCCCTGTCTTATTTGAAAGATAACCCGTCCTTGGGTGAGGACAAGCCTGTGTTTTTCAGTGTTAAAGCTTATATAAGCTCCATCAAGCCTGATCAGACTCTCTGGTACCGGGCTTGCAAAATGTGCAACAAAAAGGTGACTGAAAGTCTGGATGGTGGTTTCTGGTGTGACGGGTGCCAGAAAAATGCTGAAGAATGCAGTTTAAGGTATATATTAGTTGCAAGAGCTATGGATGCAACTAGTGAAGTATATCTTTCTGTGTTTAATAATGAAGCAGAGAGAATTATTGGGTGCTCTGCAGATGAGCTGGACAAACTGAGATCAGAGGAGCACAGTCTGTACGAGGTGAAGTTGAAACAAGCTACTTGGGTCCCTCATGTTTTCAGGGTCAGTGTTTCTCAGAATGAGTACAATAACGAGAAGAGGCAAAGGATAACTGCCAGGGCAGCTGCTCCACTTGATTTTGCTGCTGAATCAAAATTTTTGCTAGAAGAGATATCAAAGATGAAAGCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

572

Amino Acids

63.71

Weight (kDa)

6.34

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF223 PF02721 129 - 232 1.2e-07 Domain of unknown function DUF223
tRNA_anti-codon PF01336 141 - 225 2.4e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 258 - 355 4.5e-23 Replication protein A OB domain
Rep_fac-A_C PF08646 418 - 562 1.7e-44 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 217
Acc65I GGTACC 1 cut(s) 1305
AccB1I GGYRCC 2 cut(s) 1305, 1374
AccI GTMKAC 2 cut(s) 102, 1034
AclWI GGATC 1 cut(s) 70
AcoI YGGCCR 3 cut(s) 45, 366, 946
AcsI RAATTY 4 cut(s) 531, 682, 734, 1679
AcuI CTGAAG 3 cut(s) 1098, 1131, 1410
AfaI GTAC 6 cut(s) 463, 651, 1187, 1307, 1541, 1610
AfiI CCNNNNNNNGG 4 cut(s) 79, 413, 1231, 1232
AflII CTTAAG 1 cut(s) 113
AgsI TTSAA 8 cut(s) 283, 403, 499, 584, 609, 1093, 1216, 1555
AhdI GACNNNNNGTC 1 cut(s) 1344
AhlI ACTAGT 1 cut(s) 1439
AjnI CCWGG 5 cut(s) 93, 948, 1043, 1097, 1640
Alw21I GWGCWC 4 cut(s) 619, 1052, 1496, 1533
Alw26I GTCTC 3 cut(s) 208, 825, 1005
AlwI GGATC 1 cut(s) 70
AoxI GGCC 4 cut(s) 45, 348, 366, 946
ApeKI GCWGC 6 cut(s) 314, 356, 557, 1646, 1649, 1667
ApoI RAATTY 4 cut(s) 531, 682, 734, 1679
Asp700I GAANNNNTTC 1 cut(s) 579
Asp718I GGTACC 1 cut(s) 1305
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 6 cut(s) 14, 349, 423, 764, 1179, 1570
AsuC2I CCSGG 3 cut(s) 80, 353, 1310
AsuHPI GGTGA 6 cut(s) 198, 265, 313, 1247, 1348, 1558
AvaII GGWCC 5 cut(s) 14, 423, 764, 1179, 1570
BalI TGGCCA 3 cut(s) 47, 368, 948
BanI GGYRCC 2 cut(s) 1305, 1374
BanII GRGCYC 3 cut(s) 619, 1052, 1157
BbsI GAAGAC 1 cut(s) 487
Bbv12I GWGCWC 4 cut(s) 619, 1052, 1496, 1533
BbvI GCAGC 6 cut(s) 301, 343, 544, 1636, 1654, 1658
BciT130I CCWGG 5 cut(s) 95, 950, 1045, 1099, 1642
BciVI GTATCC 1 cut(s) 1036
BclI TGATCA 2 cut(s) 970, 1291
BcnI CCSGG 3 cut(s) 80, 353, 1310
BcoDI GTCTC 3 cut(s) 208, 825, 1005
BcuI ACTAGT 1 cut(s) 1439
BfaI CTAG 5 cut(s) 144, 477, 587, 1440, 1688
BfmI CTRYAG 2 cut(s) 981, 1497
BfrI CTTAAG 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 217
BfuI GTATCC 1 cut(s) 1036
BisI GCNGC 6 cut(s) 315, 357, 558, 1647, 1650, 1668
BlsI GCNGC 6 cut(s) 316, 358, 559, 1648, 1651, 1669
Bme1390I CCNGG 8 cut(s) 80, 95, 353, 950, 1045, 1099, 1310, 1642
Bme18I GGWCC 5 cut(s) 14, 423, 764, 1179, 1570
BmeRI GACNNNNNGTC 1 cut(s) 1344
BmgT120I GGNCC 6 cut(s) 14, 349, 423, 764, 1179, 1570
BmiI GGNNCC 8 cut(s) 351, 454, 765, 766, 1307, 1376, 1571, 1572
BmrFI CCNGG 8 cut(s) 80, 95, 353, 950, 1045, 1099, 1310, 1642
BmsI GCATC 5 cut(s) 16, 129, 843, 1142, 1423
BpiI GAAGAC 1 cut(s) 487
BplI GAGNNNNNCTC 2 cut(s) 188, 220
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 3 cut(s) 80, 353, 1310
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 3 cut(s) 351, 1230, 1641
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 413, 1231, 1232
Bse1I ACTGG 1 cut(s) 943
Bse3DI GCAATG 3 cut(s) 712, 882, 963
BseBI CCWGG 5 cut(s) 95, 950, 1045, 1099, 1642
BseDI CCNNGG 3 cut(s) 351, 1230, 1641
BseGI GGATG 5 cut(s) 385, 700, 799, 1357, 1438
BseLI CCNNNNNNNGG 4 cut(s) 79, 413, 1231, 1232
BseMI GCAATG 3 cut(s) 712, 882, 963
BseMII CTCAG 3 cut(s) 678, 1508, 1613
BseNI ACTGG 1 cut(s) 943
BseRI GAGGAG 1 cut(s) 1541
BseXI GCAGC 6 cut(s) 301, 343, 544, 1636, 1654, 1658
BsgI GTGCAG 1 cut(s) 342
BshFI GGCC 4 cut(s) 47, 350, 368, 948
BshNI GGYRCC 2 cut(s) 1305, 1374
BsiHKAI GWGCWC 4 cut(s) 619, 1052, 1496, 1533
BsiSI CCGG 3 cut(s) 80, 353, 1309
BslFI GGGAC 2 cut(s) 750, 1556
BslI CCNNNNNNNGG 4 cut(s) 79, 413, 1231, 1232
BsmAI GTCTC 3 cut(s) 208, 825, 1005
BsmFI GGGAC 2 cut(s) 750, 1556
BsmI GAATGC 3 cut(s) 343, 478, 1400
BsnI GGCC 4 cut(s) 47, 350, 368, 948
Bso31I GGTCTC 1 cut(s) 208
Bsp1286I GDGCHC 5 cut(s) 619, 1052, 1157, 1496, 1533
Bsp143I GATC 6 cut(s) 75, 84, 928, 970, 1291, 1520
BspANI GGCC 4 cut(s) 47, 350, 368, 948
BspCNI CTCAG 3 cut(s) 679, 1509, 1612
BspLI GGNNCC 8 cut(s) 351, 454, 765, 766, 1307, 1376, 1571, 1572
BspMAI CTGCAG 1 cut(s) 1501
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 1 cut(s) 70
BspT107I GGYRCC 2 cut(s) 1305, 1374
BspTI CTTAAG 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 208
BsrDI GCAATG 3 cut(s) 712, 882, 963
BsrI ACTGG 1 cut(s) 943
BssECI CCNNGG 3 cut(s) 351, 1230, 1641
BssMI GATC 6 cut(s) 75, 84, 928, 970, 1291, 1520
BssT1I CCWWGG 1 cut(s) 1230
Bst2UI CCWGG 5 cut(s) 95, 950, 1045, 1099, 1642
Bst4CI ACNGT 3 cut(s) 128, 649, 1535
Bst6I CTCTTC 2 cut(s) 1616, 1686
BstAFI CTTAAG 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 1323
BstC8I GCNNGC 1 cut(s) 1315
BstDEI CTNAG 3 cut(s) 687, 1517, 1599
BstENI CCTNNNNNAGG 1 cut(s) 411
BstF5I GGATG 5 cut(s) 385, 700, 799, 1357, 1438
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 6 cut(s) 78, 87, 931, 973, 1294, 1523
BstMAI GTCTC 3 cut(s) 208, 825, 1005
BstMBI GATC 6 cut(s) 75, 84, 928, 970, 1291, 1520
BstMWI GCNNNNNNNGC 6 cut(s) 347, 356, 365, 1323, 1433, 1646
BstNI CCWGG 5 cut(s) 95, 950, 1045, 1099, 1642
BstSCI CCNGG 8 cut(s) 78, 93, 351, 948, 1043, 1097, 1308, 1640
BstSFI CTRYAG 2 cut(s) 981, 1497
BstV1I GCAGC 6 cut(s) 301, 343, 544, 1636, 1654, 1658
BstV2I GAAGAC 1 cut(s) 487
BsuI GTATCC 1 cut(s) 1036
BsuRI GGCC 4 cut(s) 47, 350, 368, 948
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 5 cut(s) 385, 700, 799, 1357, 1438
BtsIMutI CAGTG 2 cut(s) 1264, 1597
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 1 cut(s) 1315
CfoI GCGC 1 cut(s) 214
Cfr13I GGNCC 6 cut(s) 14, 349, 423, 764, 1179, 1570
Csp6I GTAC 6 cut(s) 462, 650, 1186, 1306, 1540, 1609
CviAII CATG 7 cut(s) 329, 370, 457, 770, 1176, 1183, 1577
CviQI GTAC 6 cut(s) 462, 650, 1186, 1306, 1540, 1609
DdeI CTNAG 3 cut(s) 687, 1517, 1599
DpnI GATC 6 cut(s) 77, 86, 930, 972, 1293, 1522
DpnII GATC 6 cut(s) 75, 84, 928, 970, 1291, 1520
DriI GACNNNNNGTC 1 cut(s) 1344
EaeI YGGCCR 3 cut(s) 45, 366, 946
Eam1104I CTCTTC 2 cut(s) 1616, 1686
Eam1105I GACNNNNNGTC 1 cut(s) 1344
EarI CTCTTC 2 cut(s) 1616, 1686
Ecl136II GAGCTC 2 cut(s) 617, 1050
Eco130I CCWWGG 1 cut(s) 1230
Eco24I GRGCYC 3 cut(s) 619, 1052, 1157
Eco31I GGTCTC 1 cut(s) 208
Eco32I GATATC 1 cut(s) 1698
Eco47I GGWCC 5 cut(s) 14, 423, 764, 1179, 1570
Eco53kI GAGCTC 2 cut(s) 617, 1050
Eco57I CTGAAG 3 cut(s) 1098, 1131, 1410
EcoICRI GAGCTC 2 cut(s) 617, 1050
EcoNI CCTNNNNNAGG 1 cut(s) 411
EcoO109I RGGNCCY 2 cut(s) 764, 1570
EcoRI GAATTC 1 cut(s) 682
EcoRII CCWGG 5 cut(s) 93, 948, 1043, 1097, 1640
EcoRV GATATC 1 cut(s) 1698
EcoT14I CCWWGG 1 cut(s) 1230
EcoT22I ATGCAT 1 cut(s) 330
EcoT38I GRGCYC 3 cut(s) 619, 1052, 1157
ErhI CCWWGG 1 cut(s) 1230
FaeI CATG 7 cut(s) 332, 373, 460, 773, 1179, 1186, 1580
FalI AAGNNNNNCTT 2 cut(s) 1437, 1469
FaqI GGGAC 2 cut(s) 750, 1556
FatI CATG 7 cut(s) 328, 369, 456, 769, 1175, 1182, 1576
FbaI TGATCA 2 cut(s) 970, 1291
FblI GTMKAC 2 cut(s) 102, 1034
Fnu4HI GCNGC 6 cut(s) 315, 357, 558, 1647, 1650, 1668
FokI GGATG 5 cut(s) 372, 707, 806, 1364, 1445
FriOI GRGCYC 3 cut(s) 619, 1052, 1157
Fsp4HI GCNGC 6 cut(s) 315, 357, 558, 1647, 1650, 1668
FspBI CTAG 5 cut(s) 144, 477, 587, 1440, 1688
GlaI GCGC 1 cut(s) 213
GluI GCNGC 6 cut(s) 315, 357, 558, 1647, 1650, 1668
HaeIII GGCC 4 cut(s) 47, 350, 368, 948
HapII CCGG 3 cut(s) 80, 353, 1309
HhaI GCGC 1 cut(s) 214
Hin1II CATG 7 cut(s) 332, 373, 460, 773, 1179, 1186, 1580
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HincII GTYRAC 3 cut(s) 103, 510, 775
HindII GTYRAC 3 cut(s) 103, 510, 775
HindIII AAGCTT 4 cut(s) 8, 714, 1266, 1710
HinfI GANTC 7 cut(s) 65, 403, 893, 1106, 1197, 1297, 1673
HpaI GTTAAC 2 cut(s) 510, 775
HpaII CCGG 3 cut(s) 80, 353, 1309
HphI GGTGA 6 cut(s) 198, 265, 313, 1247, 1348, 1558
Hpy166II GTNNAC 5 cut(s) 103, 138, 510, 775, 1035
Hpy188I TCNGA 7 cut(s) 688, 839, 1111, 1192, 1296, 1525, 1602
Hpy188III TCNNGA 3 cut(s) 725, 1067, 1349
Hpy8I GTNNAC 5 cut(s) 103, 138, 510, 775, 1035
Hpy99I CGWCG 2 cut(s) 92, 104
HpyAV CCTTC 3 cut(s) 372, 586, 1106
HpyCH4III ACNGT 3 cut(s) 128, 649, 1535
HpyCH4IV ACGT 1 cut(s) 1037
HpyF10VI GCNNNNNNNGC 6 cut(s) 347, 356, 365, 1323, 1433, 1646
HpyF3I CTNAG 3 cut(s) 687, 1517, 1599
HpySE526I ACGT 1 cut(s) 1037
Hsp92II CATG 7 cut(s) 332, 373, 460, 773, 1179, 1186, 1580
HspAI GCGC 1 cut(s) 212
KflI GGGWCCC 2 cut(s) 764, 1570
KpnI GGTACC 1 cut(s) 1309
Ksp22I TGATCA 2 cut(s) 970, 1291
KspAI GTTAAC 2 cut(s) 510, 775
Kzo9I GATC 6 cut(s) 75, 84, 928, 970, 1291, 1520
LmnI GCTCC 6 cut(s) 614, 1047, 1172, 1283, 1528, 1657
Lsp1109I GCAGC 6 cut(s) 301, 343, 544, 1636, 1654, 1658
LweI GCATC 5 cut(s) 16, 129, 843, 1142, 1423
MaeI CTAG 5 cut(s) 144, 477, 587, 1440, 1688
MaeII ACGT 1 cut(s) 1037
MaeIII GTNAC 7 cut(s) 184, 301, 439, 808, 872, 1336, 1367
MalI GATC 6 cut(s) 77, 86, 930, 972, 1293, 1522
MboI GATC 6 cut(s) 75, 84, 928, 970, 1291, 1520
MboII GAAGA 7 cut(s) 487, 530, 722, 852, 1403, 1633, 1703
MfeI CAATTG 2 cut(s) 418, 750
MhlI GDGCHC 5 cut(s) 619, 1052, 1157, 1496, 1533
MlsI TGGCCA 3 cut(s) 47, 368, 948
MluCI AATT 9 cut(s) 321, 418, 531, 682, 734, 750, 953, 1482, 1679
MluNI TGGCCA 3 cut(s) 47, 368, 948
MlyI GAGTC 3 cut(s) 74, 902, 1291
MmeI TCCRAC 1 cut(s) 988
Mox20I TGGCCA 3 cut(s) 47, 368, 948
Mph1103I ATGCAT 1 cut(s) 330
MroXI GAANNNNTTC 1 cut(s) 579
MscI TGGCCA 3 cut(s) 47, 368, 948
Msp20I TGGCCA 3 cut(s) 47, 368, 948
MspA1I CMGCKG 2 cut(s) 314, 1649
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 3 cut(s) 80, 353, 1309
MspR9I CCNGG 8 cut(s) 80, 95, 353, 950, 1045, 1099, 1310, 1642
MunI CAATTG 2 cut(s) 418, 750
Mva1269I GAATGC 3 cut(s) 343, 478, 1400
MvaI CCWGG 5 cut(s) 95, 950, 1045, 1099, 1642
MwoI GCNNNNNNNGC 6 cut(s) 347, 356, 365, 1323, 1433, 1646
NciI CCSGG 3 cut(s) 80, 353, 1310
NdeII GATC 6 cut(s) 75, 84, 928, 970, 1291, 1520
NlaIII CATG 7 cut(s) 332, 373, 460, 773, 1179, 1186, 1580
NlaIV GGNNCC 8 cut(s) 351, 454, 765, 766, 1307, 1376, 1571, 1572
NmuCI GTSAC 4 cut(s) 301, 872, 1336, 1367
NsiI ATGCAT 1 cut(s) 330
PaqCI CACCTGC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 3 cut(s) 343, 478, 1400
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 4 cut(s) 403, 1106, 1197, 1673
PfoI TCCNGGA 2 cut(s) 78, 1043
PkrI GCNGC 6 cut(s) 316, 358, 559, 1648, 1651, 1669
PleI GAGTC 3 cut(s) 73, 901, 1291
PpsI GAGTC 3 cut(s) 73, 901, 1291
PpuMI RGGWCCY 2 cut(s) 764, 1570
Psp124BI GAGCTC 2 cut(s) 619, 1052
Psp5II RGGWCCY 2 cut(s) 764, 1570
Psp6I CCWGG 5 cut(s) 93, 948, 1043, 1097, 1640
PspGI CCWGG 5 cut(s) 93, 948, 1043, 1097, 1640
PspN4I GGNNCC 8 cut(s) 351, 454, 765, 766, 1307, 1376, 1571, 1572
PspPI GGNCC 6 cut(s) 14, 349, 423, 764, 1179, 1570
PspPPI RGGWCCY 2 cut(s) 764, 1570
PsrI GAACNNNNNNTAC 2 cut(s) 445, 477
PstI CTGCAG 1 cut(s) 1501
PvuII CAGCTG 2 cut(s) 314, 1649
RsaI GTAC 6 cut(s) 463, 651, 1187, 1307, 1541, 1610
RsaNI GTAC 6 cut(s) 462, 650, 1186, 1306, 1540, 1609
SacI GAGCTC 2 cut(s) 619, 1052
SalI GTCGAC 1 cut(s) 101
SatI GCNGC 6 cut(s) 315, 357, 558, 1647, 1650, 1668
Sau3AI GATC 6 cut(s) 75, 84, 928, 970, 1291, 1520
Sau96I GGNCC 6 cut(s) 14, 349, 423, 764, 1179, 1570
SchI GAGTC 3 cut(s) 74, 902, 1291
ScrFI CCNGG 8 cut(s) 80, 95, 353, 950, 1045, 1099, 1310, 1642
SduI GDGCHC 5 cut(s) 619, 1052, 1157, 1496, 1533
SfaNI GCATC 5 cut(s) 16, 129, 843, 1142, 1423
SfcI CTRYAG 2 cut(s) 981, 1497
SinI GGWCC 5 cut(s) 14, 423, 764, 1179, 1570
SmlI CTYRAG 2 cut(s) 113, 215
SmoI CTYRAG 2 cut(s) 113, 215
SpeI ACTAGT 1 cut(s) 1439
Sse9I AATT 9 cut(s) 321, 418, 531, 682, 734, 750, 953, 1482, 1679
SspI AATATT 1 cut(s) 1056
SspMI CTAG 5 cut(s) 144, 477, 587, 1440, 1688
SstI GAGCTC 2 cut(s) 619, 1052
StyD4I CCNGG 8 cut(s) 78, 93, 351, 948, 1043, 1097, 1308, 1640
StyI CCWWGG 1 cut(s) 1230
TaaI ACNGT 3 cut(s) 128, 649, 1535
TaiI ACGT 1 cut(s) 1040
TaqI TCGA 6 cut(s) 35, 102, 470, 535, 896, 1127
TasI AATT 9 cut(s) 321, 418, 531, 682, 734, 750, 953, 1482, 1679
TatI WGTACW 3 cut(s) 649, 1185, 1608
TfiI GAWTC 4 cut(s) 403, 1106, 1197, 1673
TscAI CASTG 2 cut(s) 1264, 1597
TseFI GTSAC 4 cut(s) 301, 872, 1336, 1367
TseI GCWGC 6 cut(s) 314, 356, 557, 1646, 1649, 1667
Tsp45I GTSAC 4 cut(s) 301, 872, 1336, 1367
TspDTI ATGAA 8 cut(s) 374, 531, 567, 588, 678, 711, 723, 1485
TspRI CASTG 2 cut(s) 1264, 1597
Vha464I CTTAAG 1 cut(s) 113
VpaK11BI GGWCC 5 cut(s) 14, 423, 764, 1179, 1570
XagI CCTNNNNNAGG 1 cut(s) 411
XapI RAATTY 4 cut(s) 531, 682, 734, 1679
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XmiI GTMKAC 2 cut(s) 102, 1034
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 5 cut(s) 144, 477, 587, 1440, 1688
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.