Rroxscaffold_3G00270700

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009976
Physical Location & Seq
Reverse (-)
63248358 .. 63251799
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270700.1

Sequence Viewer

Length: 1827 bp
ATGGCGAAGCTTGGACCAACCCCAGATGCGATTTCGACCGTCTTGGCCAACCCATCGCCGGACCGGTCCAGCCCCATCCCGGAGATCGTCGTCCAGGTCGTCGATCTCAATCTTAAGGGCAACAACCGTTATATGTTTACTGCTACTGATGGAAAGATGAGGCTAAAAGCGATGTTGAACTCAACTCTGAGTCCTGATGTTATTTCGGGGAAAATTCAGAACTTGGGCCTCATTCGTGTTCTTGATTACGCTGTTAATGACATTCCAAACAAGCCTGACAAATATTTGATTGTAACAAAATGTGAGATGGTTTCACCTGCGCTTGAGATGGAGATTAAAAGTGAGGTCAGTAAAGATGAGGTGAAAGGAGAACAGGTTGGGATTATTTTGAAGCCCAAGCAAGAGATGGTGACTAAATCAGCTGCTCAAATTATGCATGAGCAGAATGCAAATATGGCCCCGGCTGCACGAATGGCCATGACCAGAAGGGTTCATCCTATTGTTTCCTTGAATCCTTACCAAGGCAATTGGACCATAAAGGTTCGTGTTACAAACAAGGGAACCATGCATACTTACTCGAATGCTAGAGGTCAAGGCTGTGTCTTCAACGTGGAGTTGACAGATGAAGATGGAACTGAAATTAGAGCTACAATGTTTAATGAAGCTGCAAAGAAGTTCTATGAAAAGTTTCAACTAGGGAAGGTCTATTATGTTTCAAAGGGAGCTCTAAAGGTTGCTAACAAGAAGTTTAGTACAGTGAAAAATGATTATGAAATGACCTTAAATGAGAATTCTGAGGTGGATGAAGTCAGCAATGAAGAAGCTTTTGTTCCTGAAACAAAATTTGATTTTATCCCAATTGATATGTTGGGTCCCCATGTTAACAGCAAGGTGCTTGTGGATGTTATTGGAGTTGTTACAAATGTGTCTCCTACAATGAGCATCAGAAGAAAGAGCAACAATGAGAATATCCCAAAGCGTGACATTACCATTGCTGATGAGTCGAAAAAAATAGTTGTGGTTTCTTTATGGAATGATCTTGCAACTGGTGTTGGCCAGGAATTGCTGGACATTGCTGATCAATCTCCCGTAGTTGCTATCAAATCCCTTAAAGTTGGAGACTATCAAGGTGTATCCCTGTCTACGTTGTCCAGGAGCTCAATATTTGTAAATCCAGATTTACCTGAAGCAAAGAGGTTGAAATCCTGGTATGATTCTGAAGGTAAAGAGGTTTCAATGGCATCTGTTGGCTCTGGTATGAGCCCATCAGCCAATAGTGGAGCATGGTCCATGTACTCTAATAGAATCTCCCTGTCTTATTTGAAAGATAACCCATCCTTGGGTGAGGACAAGCCTGTGTTTTTCAGTGTTAAAGCTTATATAAGCTCCATCAAGCCTGATCAGACTCTCTGGTACCGGGCTTGCAAAATGTGCAACAAAAAGGTGACTGAAAGTCTGGATGGTGGTTTCTGGTGTGACGGGTGCCAGAAAAATGCTGAAGAATGCAGTTTAAGGTATATATTAGTTGCAAGAGCTATGGATGCAACTAGTGAAGTATATCTTTCTGTGTTTAATAATGAAGCAGAAAGGATTATTGGGTGCTCTGCAGATGAGCTGGACAAACTGAGATCAGAGGAGCACATTCTGTATGAGGTGAAGTTGAAACAAGCTACTTGGGTTCCTCATCTTTTCAGGGTCAGTGTTTCTCAGAATGAGTACAATAACGAAAAGAGGCAAAGGATAACTGCCAGGGCAGCTGCTCCACTTGATTTTGCTGCTGAATCAAAATTTTTGCTAGAAGAGATATCAAAGATGAAAGCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

608

Amino Acids

67.63

Weight (kDa)

7.01

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 7 - 105 1.9e-20 Replication factor-A protein 1, N-terminal domain
DUF223 PF02721 165 - 268 5e-08 Domain of unknown function DUF223
tRNA_anti-codon PF01336 177 - 262 2.6e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 294 - 391 2.7e-23 Replication protein A OB domain
Rep_fac-A_C PF08646 454 - 598 4.1e-44 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 1 cut(s) 325
Acc65I GGTACC 1 cut(s) 1413
AccB1I GGYRCC 2 cut(s) 1413, 1482
AccI GTMKAC 1 cut(s) 1142
AcoI YGGCCR 3 cut(s) 45, 474, 1054
AcsI RAATTY 4 cut(s) 213, 790, 842, 1787
AcuI CTGAAG 3 cut(s) 1206, 1239, 1518
AfaI GTAC 4 cut(s) 754, 1295, 1415, 1718
AfiI CCNNNNNNNGG 5 cut(s) 58, 79, 521, 1339, 1340
AflII CTTAAG 1 cut(s) 113
AgeI ACCGGT 1 cut(s) 63
AhdI GACNNNNNGTC 1 cut(s) 1452
AhlI ACTAGT 1 cut(s) 1547
AjnI CCWGG 5 cut(s) 93, 1056, 1151, 1205, 1748
AjuI GAANNNNNNNTTGG 2 cut(s) 1578, 1610
Alw21I GWGCWC 4 cut(s) 727, 1160, 1604, 1641
Alw26I GTCTC 2 cut(s) 933, 1113
AoxI GGCC 5 cut(s) 45, 226, 456, 474, 1054
ApeKI GCWGC 6 cut(s) 422, 464, 665, 1754, 1757, 1775
ApoI RAATTY 4 cut(s) 213, 790, 842, 1787
AsiGI ACCGGT 1 cut(s) 63
Asp700I GAANNNNTTC 1 cut(s) 687
Asp718I GGTACC 1 cut(s) 1413
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 8 cut(s) 14, 61, 66, 226, 457, 531, 872, 1287
AsuC2I CCSGG 3 cut(s) 80, 461, 1418
AsuHPI GGTGA 6 cut(s) 306, 373, 421, 1355, 1456, 1666
AvaII GGWCC 6 cut(s) 14, 61, 66, 531, 872, 1287
BalI TGGCCA 3 cut(s) 47, 476, 1056
BanI GGYRCC 2 cut(s) 1413, 1482
BanII GRGCYC 3 cut(s) 727, 1160, 1265
BbsI GAAGAC 1 cut(s) 595
Bbv12I GWGCWC 4 cut(s) 727, 1160, 1604, 1641
BbvI GCAGC 6 cut(s) 409, 451, 652, 1744, 1762, 1766
BciT130I CCWGG 5 cut(s) 95, 1058, 1153, 1207, 1750
BciVI GTATCC 1 cut(s) 1144
BclI TGATCA 2 cut(s) 1078, 1399
BcnI CCSGG 3 cut(s) 80, 461, 1418
BcoDI GTCTC 2 cut(s) 933, 1113
BcuI ACTAGT 1 cut(s) 1547
BfaI CTAG 4 cut(s) 585, 695, 1548, 1796
BfmI CTRYAG 1 cut(s) 1605
BfrI CTTAAG 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 325
BfuI GTATCC 1 cut(s) 1144
BisI GCNGC 6 cut(s) 423, 465, 666, 1755, 1758, 1776
BlsI GCNGC 6 cut(s) 424, 466, 667, 1756, 1759, 1777
Bme1390I CCNGG 8 cut(s) 80, 95, 461, 1058, 1153, 1207, 1418, 1750
Bme18I GGWCC 6 cut(s) 14, 61, 66, 531, 872, 1287
BmeRI GACNNNNNGTC 1 cut(s) 1452
BmgT120I GGNCC 8 cut(s) 14, 61, 66, 226, 457, 531, 872, 1287
BmiI GGNNCC 7 cut(s) 459, 562, 873, 874, 1415, 1484, 1680
BmrFI CCNGG 8 cut(s) 80, 95, 461, 1058, 1153, 1207, 1418, 1750
BmsI GCATC 4 cut(s) 16, 951, 1250, 1531
BpiI GAAGAC 1 cut(s) 595
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 3 cut(s) 80, 461, 1418
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 459, 520, 1338, 1749
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 79, 521, 1339, 1340
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 1 cut(s) 1051
Bse3DI GCAATG 3 cut(s) 820, 990, 1071
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 5 cut(s) 95, 1058, 1153, 1207, 1750
BseDI CCNNGG 4 cut(s) 459, 520, 1338, 1749
BseGI GGATG 7 cut(s) 75, 493, 808, 907, 1334, 1465, 1546
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 5 cut(s) 58, 79, 521, 1339, 1340
BseMI GCAATG 3 cut(s) 820, 990, 1071
BseMII CTCAG 4 cut(s) 179, 786, 1616, 1721
BseNI ACTGG 1 cut(s) 1051
BseRI GAGGAG 1 cut(s) 1649
BseXI GCAGC 6 cut(s) 409, 451, 652, 1744, 1762, 1766
BsgI GTGCAG 1 cut(s) 450
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 5 cut(s) 47, 228, 458, 476, 1056
BshNI GGYRCC 2 cut(s) 1413, 1482
BshTI ACCGGT 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 4 cut(s) 727, 1160, 1604, 1641
BsiSI CCGG 5 cut(s) 59, 64, 80, 461, 1417
BslFI GGGAC 1 cut(s) 858
BslI CCNNNNNNNGG 5 cut(s) 58, 79, 521, 1339, 1340
BsmAI GTCTC 2 cut(s) 933, 1113
BsmFI GGGAC 1 cut(s) 858
BsmI GAATGC 3 cut(s) 451, 586, 1508
BsnI GGCC 5 cut(s) 47, 228, 458, 476, 1056
Bsp1286I GDGCHC 5 cut(s) 727, 1160, 1265, 1604, 1641
Bsp143I GATC 6 cut(s) 84, 103, 1036, 1078, 1399, 1628
BspANI GGCC 5 cut(s) 47, 228, 458, 476, 1056
BspCNI CTCAG 4 cut(s) 180, 787, 1617, 1720
BspLI GGNNCC 7 cut(s) 459, 562, 873, 874, 1415, 1484, 1680
BspMAI CTGCAG 1 cut(s) 1609
BspMI ACCTGC 1 cut(s) 325
BspT107I GGYRCC 2 cut(s) 1413, 1482
BspTI CTTAAG 1 cut(s) 113
BsrDI GCAATG 3 cut(s) 820, 990, 1071
BsrFI RCCGGY 1 cut(s) 63
BsrI ACTGG 1 cut(s) 1051
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 4 cut(s) 459, 520, 1338, 1749
BssMI GATC 6 cut(s) 84, 103, 1036, 1078, 1399, 1628
BssT1I CCWWGG 2 cut(s) 520, 1338
Bst2UI CCWGG 5 cut(s) 95, 1058, 1153, 1207, 1750
Bst4CI ACNGT 3 cut(s) 40, 128, 757
Bst6I CTCTTC 1 cut(s) 1794
BstAFI CTTAAG 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 1431
BstC8I GCNNGC 1 cut(s) 1423
BstDEI CTNAG 4 cut(s) 188, 795, 1625, 1707
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 7 cut(s) 75, 493, 808, 907, 1334, 1465, 1546
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 6 cut(s) 87, 106, 1039, 1081, 1402, 1631
BstMAI GTCTC 2 cut(s) 933, 1113
BstMBI GATC 6 cut(s) 84, 103, 1036, 1078, 1399, 1628
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 6 cut(s) 455, 464, 473, 1431, 1541, 1754
BstNI CCWGG 5 cut(s) 95, 1058, 1153, 1207, 1750
BstSCI CCNGG 8 cut(s) 78, 93, 459, 1056, 1151, 1205, 1416, 1748
BstSFI CTRYAG 1 cut(s) 1605
BstV1I GCAGC 6 cut(s) 409, 451, 652, 1744, 1762, 1766
BstV2I GAAGAC 1 cut(s) 595
BsuI GTATCC 1 cut(s) 1144
BsuRI GGCC 5 cut(s) 47, 228, 458, 476, 1056
BtgZI GCGATG 2 cut(s) 39, 185
BtsCI GGATG 7 cut(s) 75, 493, 808, 907, 1334, 1465, 1546
BtsIMutI CAGTG 3 cut(s) 762, 1372, 1705
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 1423
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 8 cut(s) 14, 61, 66, 226, 457, 531, 872, 1287
CpoI CGGWCCG 1 cut(s) 61
Csp6I GTAC 4 cut(s) 753, 1294, 1414, 1717
CspAI ACCGGT 1 cut(s) 63
CspI CGGWCCG 1 cut(s) 61
CviAII CATG 6 cut(s) 437, 478, 565, 878, 1284, 1291
CviQI GTAC 4 cut(s) 753, 1294, 1414, 1717
DdeI CTNAG 4 cut(s) 188, 795, 1625, 1707
DpnI GATC 6 cut(s) 86, 105, 1038, 1080, 1401, 1630
DpnII GATC 6 cut(s) 84, 103, 1036, 1078, 1399, 1628
DriI GACNNNNNGTC 1 cut(s) 1452
EaeI YGGCCR 3 cut(s) 45, 474, 1054
Eam1104I CTCTTC 1 cut(s) 1794
Eam1105I GACNNNNNGTC 1 cut(s) 1452
EarI CTCTTC 1 cut(s) 1794
Ecl136II GAGCTC 2 cut(s) 725, 1158
Eco130I CCWWGG 2 cut(s) 520, 1338
Eco24I GRGCYC 3 cut(s) 727, 1160, 1265
Eco32I GATATC 1 cut(s) 1806
Eco47I GGWCC 6 cut(s) 14, 61, 66, 531, 872, 1287
Eco53kI GAGCTC 2 cut(s) 725, 1158
Eco57I CTGAAG 3 cut(s) 1206, 1239, 1518
EcoICRI GAGCTC 2 cut(s) 725, 1158
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 872
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 5 cut(s) 93, 1056, 1151, 1205, 1748
EcoRV GATATC 1 cut(s) 1806
EcoT14I CCWWGG 2 cut(s) 520, 1338
EcoT22I ATGCAT 2 cut(s) 438, 570
EcoT38I GRGCYC 3 cut(s) 727, 1160, 1265
ErhI CCWWGG 2 cut(s) 520, 1338
FaeI CATG 6 cut(s) 440, 481, 568, 881, 1287, 1294
FalI AAGNNNNNCTT 2 cut(s) 1545, 1577
FaqI GGGAC 1 cut(s) 858
FatI CATG 6 cut(s) 436, 477, 564, 877, 1283, 1290
FbaI TGATCA 2 cut(s) 1078, 1399
FblI GTMKAC 1 cut(s) 1142
Fnu4HI GCNGC 6 cut(s) 423, 465, 666, 1755, 1758, 1776
FokI GGATG 7 cut(s) 62, 480, 815, 914, 1321, 1472, 1553
FriOI GRGCYC 3 cut(s) 727, 1160, 1265
Fsp4HI GCNGC 6 cut(s) 423, 465, 666, 1755, 1758, 1776
FspBI CTAG 4 cut(s) 585, 695, 1548, 1796
GlaI GCGC 1 cut(s) 321
GluI GCNGC 6 cut(s) 423, 465, 666, 1755, 1758, 1776
HaeIII GGCC 5 cut(s) 47, 228, 458, 476, 1056
HapII CCGG 5 cut(s) 59, 64, 80, 461, 1417
HhaI GCGC 1 cut(s) 322
Hin1II CATG 6 cut(s) 440, 481, 568, 881, 1287, 1294
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HincII GTYRAC 2 cut(s) 618, 883
HindII GTYRAC 2 cut(s) 618, 883
HindIII AAGCTT 4 cut(s) 8, 822, 1374, 1818
HinfI GANTC 7 cut(s) 190, 511, 1001, 1214, 1305, 1405, 1781
HpaI GTTAAC 1 cut(s) 883
HpaII CCGG 5 cut(s) 59, 64, 80, 461, 1417
HphI GGTGA 6 cut(s) 306, 373, 421, 1355, 1456, 1666
Hpy166II GTNNAC 4 cut(s) 138, 618, 883, 1143
Hpy188I TCNGA 8 cut(s) 189, 219, 796, 947, 1219, 1404, 1633, 1710
Hpy188III TCNNGA 5 cut(s) 194, 242, 833, 1175, 1457
Hpy8I GTNNAC 4 cut(s) 138, 618, 883, 1143
Hpy99I CGWCG 2 cut(s) 92, 104
HpyAV CCTTC 3 cut(s) 480, 694, 1214
HpyCH4III ACNGT 3 cut(s) 40, 128, 757
HpyCH4IV ACGT 2 cut(s) 609, 1145
HpyF10VI GCNNNNNNNGC 6 cut(s) 455, 464, 473, 1431, 1541, 1754
HpyF3I CTNAG 4 cut(s) 188, 795, 1625, 1707
HpySE526I ACGT 2 cut(s) 609, 1145
Hsp92II CATG 6 cut(s) 440, 481, 568, 881, 1287, 1294
HspAI GCGC 1 cut(s) 320
KflI GGGWCCC 1 cut(s) 872
KpnI GGTACC 1 cut(s) 1417
Ksp22I TGATCA 2 cut(s) 1078, 1399
KspAI GTTAAC 1 cut(s) 883
Kzo9I GATC 6 cut(s) 84, 103, 1036, 1078, 1399, 1628
LmnI GCTCC 6 cut(s) 722, 1155, 1280, 1391, 1636, 1765
Lsp1109I GCAGC 6 cut(s) 409, 451, 652, 1744, 1762, 1766
LweI GCATC 4 cut(s) 16, 951, 1250, 1531
MaeI CTAG 4 cut(s) 585, 695, 1548, 1796
MaeII ACGT 2 cut(s) 609, 1145
MaeIII GTNAC 7 cut(s) 292, 409, 547, 916, 980, 1444, 1475
MalI GATC 6 cut(s) 86, 105, 1038, 1080, 1401, 1630
MboI GATC 6 cut(s) 84, 103, 1036, 1078, 1399, 1628
MboII GAAGA 6 cut(s) 595, 638, 830, 960, 1511, 1811
MfeI CAATTG 2 cut(s) 526, 858
MhlI GDGCHC 5 cut(s) 727, 1160, 1265, 1604, 1641
MlsI TGGCCA 3 cut(s) 47, 476, 1056
MluCI AATT 9 cut(s) 213, 429, 526, 639, 790, 842, 858, 1061, 1787
MluNI TGGCCA 3 cut(s) 47, 476, 1056
MlyI GAGTC 3 cut(s) 199, 1010, 1399
MmeI TCCRAC 1 cut(s) 1096
Mox20I TGGCCA 3 cut(s) 47, 476, 1056
Mph1103I ATGCAT 2 cut(s) 438, 570
MroXI GAANNNNTTC 1 cut(s) 687
MscI TGGCCA 3 cut(s) 47, 476, 1056
Msp20I TGGCCA 3 cut(s) 47, 476, 1056
MspA1I CMGCKG 2 cut(s) 422, 1757
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 5 cut(s) 59, 64, 80, 461, 1417
MspR9I CCNGG 8 cut(s) 80, 95, 461, 1058, 1153, 1207, 1418, 1750
MunI CAATTG 2 cut(s) 526, 858
Mva1269I GAATGC 3 cut(s) 451, 586, 1508
MvaI CCWGG 5 cut(s) 95, 1058, 1153, 1207, 1750
MwoI GCNNNNNNNGC 6 cut(s) 455, 464, 473, 1431, 1541, 1754
NciI CCSGG 3 cut(s) 80, 461, 1418
NdeII GATC 6 cut(s) 84, 103, 1036, 1078, 1399, 1628
NlaIII CATG 6 cut(s) 440, 481, 568, 881, 1287, 1294
NlaIV GGNNCC 7 cut(s) 459, 562, 873, 874, 1415, 1484, 1680
NmuCI GTSAC 4 cut(s) 409, 980, 1444, 1475
NsiI ATGCAT 2 cut(s) 438, 570
PaqCI CACCTGC 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 3 cut(s) 451, 586, 1508
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 4 cut(s) 511, 1214, 1305, 1781
PfoI TCCNGGA 2 cut(s) 78, 1151
PinAI ACCGGT 1 cut(s) 63
PkrI GCNGC 6 cut(s) 424, 466, 667, 1756, 1759, 1777
PleI GAGTC 3 cut(s) 198, 1009, 1399
PpsI GAGTC 3 cut(s) 198, 1009, 1399
PpuMI RGGWCCY 1 cut(s) 872
Psp124BI GAGCTC 2 cut(s) 727, 1160
Psp5II RGGWCCY 1 cut(s) 872
Psp6I CCWGG 5 cut(s) 93, 1056, 1151, 1205, 1748
PspGI CCWGG 5 cut(s) 93, 1056, 1151, 1205, 1748
PspN4I GGNNCC 7 cut(s) 459, 562, 873, 874, 1415, 1484, 1680
PspPI GGNCC 8 cut(s) 14, 61, 66, 226, 457, 531, 872, 1287
PspPPI RGGWCCY 1 cut(s) 872
PsrI GAACNNNNNNTAC 2 cut(s) 553, 585
PstI CTGCAG 1 cut(s) 1609
PvuII CAGCTG 2 cut(s) 422, 1757
RsaI GTAC 4 cut(s) 754, 1295, 1415, 1718
RsaNI GTAC 4 cut(s) 753, 1294, 1414, 1717
Rsr2I CGGWCCG 1 cut(s) 61
RsrII CGGWCCG 1 cut(s) 61
SacI GAGCTC 2 cut(s) 727, 1160
SatI GCNGC 6 cut(s) 423, 465, 666, 1755, 1758, 1776
Sau3AI GATC 6 cut(s) 84, 103, 1036, 1078, 1399, 1628
Sau96I GGNCC 8 cut(s) 14, 61, 66, 226, 457, 531, 872, 1287
SchI GAGTC 3 cut(s) 199, 1010, 1399
ScrFI CCNGG 8 cut(s) 80, 95, 461, 1058, 1153, 1207, 1418, 1750
SduI GDGCHC 5 cut(s) 727, 1160, 1265, 1604, 1641
SfaNI GCATC 4 cut(s) 16, 951, 1250, 1531
SfcI CTRYAG 1 cut(s) 1605
SinI GGWCC 6 cut(s) 14, 61, 66, 531, 872, 1287
SmlI CTYRAG 2 cut(s) 113, 323
SmoI CTYRAG 2 cut(s) 113, 323
SpeI ACTAGT 1 cut(s) 1547
Sse9I AATT 9 cut(s) 213, 429, 526, 639, 790, 842, 858, 1061, 1787
SspI AATATT 2 cut(s) 284, 1164
SspMI CTAG 4 cut(s) 585, 695, 1548, 1796
SstI GAGCTC 2 cut(s) 727, 1160
StyD4I CCNGG 8 cut(s) 78, 93, 459, 1056, 1151, 1205, 1416, 1748
StyI CCWWGG 2 cut(s) 520, 1338
TaaI ACNGT 3 cut(s) 40, 128, 757
TaiI ACGT 2 cut(s) 612, 1148
TaqI TCGA 4 cut(s) 35, 102, 578, 1004
TasI AATT 9 cut(s) 213, 429, 526, 639, 790, 842, 858, 1061, 1787
TatI WGTACW 3 cut(s) 752, 1293, 1716
TfiI GAWTC 4 cut(s) 511, 1214, 1305, 1781
TscAI CASTG 3 cut(s) 762, 1372, 1705
TseFI GTSAC 4 cut(s) 409, 980, 1444, 1475
TseI GCWGC 6 cut(s) 422, 464, 665, 1754, 1757, 1775
Tsp45I GTSAC 4 cut(s) 409, 980, 1444, 1475
TspDTI ATGAA 8 cut(s) 482, 639, 675, 696, 786, 819, 831, 1593
TspRI CASTG 3 cut(s) 762, 1372, 1705
Vha464I CTTAAG 1 cut(s) 113
VpaK11BI GGWCC 6 cut(s) 14, 61, 66, 531, 872, 1287
XagI CCTNNNNNAGG 1 cut(s) 519
XapI RAATTY 4 cut(s) 213, 790, 842, 1787
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 1142
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 4 cut(s) 585, 695, 1548, 1796
Zsp2I ATGCAT 2 cut(s) 438, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.