MD14G1212400.v1.1

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009976
Physical Location & Seq
Forward (+)
29720328 .. 29723674
3347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1212400.v1.1.491

Sequence Viewer

Length: 1815 bp
ATGGCGAAGTTAATGCCGACCCCAGATGCGATTTCGACCGTCTTGGCGAACAAGTCGCCGGACGAGTCGGTTGAGCTGCCGGAGATAGTGGTCCAGGTCTCCGATCTCACCCCTAGGGGAAAATCTTACATGTTTATTGCTACTGATGGAAATGCGAAGCTAAAGGGGATGCTTTCCTCGCGGCTGGGTCCCCAGGTCACATCTGGGGAGATTCAGAATCTGGGTCTCATTCGCGTTCTCGGTTATTCTGTTAATTTCAATTCAAACTTATCCGAAAATTATCTGCTTGTAACAAAATGTGATGTGGTTTCGGCTGCACTTGAGGCGGAGGTGAAAAATGAGGTGAAAAAGGAAGATGTTGGGATTATTTTGAAGCCCAAGCAAGAAATGGTTTCGAAATCTGCTGCTCAAATTGTGAATGAGCAGGCTGGGAATATGGCTCCGTCTGCGAGAATGGCTATGACACGAAGGGTTCACCCGCTTGTTTCCTTGAACCCTTACCAAGGAAATTGGACCATAAAGGTTCGTGTTACAAACAAGGGTACGATGCGTACATACAAGAATGCTAGAGGTGAAGGTTGCGTCTTCAATGTGGAGTTAACAGATGAAGATGGAACACAAATTCAAGCAACAATGTTTAATGAAGCTGCAAAGAAGTTCTTTGAAAAGTTTGAGTTAGGAAAGGTTTATTACATTTCAAAGGGGTCTCTGCGGGTTGCTAACAAGCAGTTTAAGACAGTGCAAAATGATTATGAAATGACATTAAATGAGTATTCTGAGGTGGAAGAAGCTAGCAATGAAGCGGCCTTTGTTCCCGAAACAAAATTCAATTTTGTTCCAGTTGATATGTTGGGTCCCCATGTTAATGGAAAGGAGCTTGTGGATGTTATTGGAGTTGTTCAAAATGTGTCTCCTACAATGAGCATTAGAAGGAAGATCGACAATGAGAGTATTCCAAAGCGTGATATCACCATTGCTGATGATACGAAGAAGACAGTTGTGGTTGCTTTGTGGGGTGAGCTTGCAACTAGTGTAGGCCAGCAATTGCTTGACATCGCTGATCAATCTCCCATAGTCGCTATCAAGTCCCTCAAAGTTGGAGACTTCCAAGGTGTATCATTGTCAACACTGAGCAAGAGCACAATATTGGTAAATCCAGAATCACCTGAGGCAAAGAAGTTGCGGTCCTGGTATGATTCTGAAGGTAAAGGTGCTTCATTGGCCTCTGTTGGCTCTGGTATGAGTCCATCAGCCAAGAGTGGAGGAAGGTCCATGTACTCTGACAAAGTCACCCTGTCTCATATATCTGAGGACCCGTCCTTGGGTGAGGATAAGCCTGTGTTTTTCAGTGTTAAAGCGTTCATAAGTTCAATCAGGCCTGATCAGGCAATGTGGTACCGCGCTTGCAAAACTTGCAACAAGAAGGTGACTGAAGCTATTGGTTCTGGATACTGGTGTGAAGGGTGCCAGAAAAATGATGAAGAATGCAGTTTAAGGTATATATCAGTTGCAAGAGTTACTGATGTAAGTGGGGAAACATATCTTTCTCTATTCAACGACGAAGCTGAGAGGGTCATCGGGTGCTCTGCAGATGAACTGGACAAATTGAAATCACAGGACGGAGACGAGAATGAATTCCAACAAAAACTGAAAGAAGCAACTTGGGTTCCTCATCTTTTTAGGGTCAGTGTTACTCTGAATGAGTACAATAACGAGAAGCGGAAGAGGATAACTGCCAGGGCAGTCGCTCCGGTTGATTTTGCTGCTGAATCGAGGTTTTTGCTTGAAGAGATATCGAAGATGAAGGCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

605

Amino Acids

66.61

Weight (kDa)

5.93

Isoelectric Point (pI)

32.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 7 - 104 9.9e-14 Replication factor-A protein 1, N-terminal domain
DUF223 PF02721 159 - 263 1.2e-09 Domain of unknown function DUF223
tRNA_anti-codon PF01336 171 - 259 1.8e-10 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 288 - 385 1.7e-23 Replication protein A OB domain
Rep_fac-A_C PF08646 448 - 594 8.6e-44 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1395
AccB1I GGYRCC 2 cut(s) 1395, 1464
AccII CGCG 3 cut(s) 181, 234, 1401
AciI CCGC 8 cut(s) 181, 326, 479, 712, 803, 1183, 1399, 1720
AcsI RAATTY 3 cut(s) 621, 824, 1634
AcuI CTGAAG 2 cut(s) 1221, 1452
AfaI GTAC 5 cut(s) 544, 553, 1277, 1397, 1706
AfiI CCNNNNNNNGG 3 cut(s) 503, 1321, 1322
AflIII ACRYGT 1 cut(s) 129
AhlI ACTAGT 1 cut(s) 1028
AjnI CCWGG 4 cut(s) 93, 192, 1187, 1736
AluBI AGCT 8 cut(s) 76, 160, 647, 791, 877, 1021, 1436, 1565
AluI AGCT 8 cut(s) 76, 160, 647, 791, 877, 1021, 1436, 1565
Alw21I GWGCWC 2 cut(s) 1142, 1586
Alw26I GTCTC 7 cut(s) 103, 230, 711, 915, 1095, 1302, 1617
AlwNI CAGNNNCTG 1 cut(s) 220
AoxI GGCC 5 cut(s) 804, 1036, 1221, 1376, 1806
ApeKI GCWGC 5 cut(s) 76, 314, 404, 647, 1763
ApoI RAATTY 3 cut(s) 621, 824, 1634
Asp700I GAANNNNTTC 1 cut(s) 1634
Asp718I GGTACC 1 cut(s) 1395
AspA2I CCTAGG 1 cut(s) 113
AspLEI GCGC 1 cut(s) 1403
AspS9I GGNCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
AsuII TTCGAA 1 cut(s) 395
AsuNHI GCTAGC 1 cut(s) 791
AvaII GGWCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
AvrII CCTAGG 1 cut(s) 113
AxyI CCTNAGG 1 cut(s) 1167
BaeI ACNNNNGTAYC 2 cut(s) 1441, 1474
BanI GGYRCC 2 cut(s) 1395, 1464
BarI GAAGNNNNNNTAC 2 cut(s) 1198, 1230
BbsI GAAGAC 2 cut(s) 577, 998
Bbv12I GWGCWC 2 cut(s) 1142, 1586
BbvI GCAGC 5 cut(s) 63, 301, 391, 634, 1750
BccI CCATC 3 cut(s) 140, 605, 1255
BcgI CGANNNNNNTGC 3 cut(s) 29, 1762, 1796
BciT130I CCWGG 4 cut(s) 95, 194, 1189, 1738
BciVI GTATCC 1 cut(s) 1442
BclI TGATCA 2 cut(s) 1060, 1381
BcoDI GTCTC 7 cut(s) 103, 230, 711, 915, 1095, 1302, 1617
BcuI ACTAGT 1 cut(s) 1028
BfaI CTAG 4 cut(s) 114, 567, 792, 1029
BfmI CTRYAG 1 cut(s) 1587
BfuI GTATCC 1 cut(s) 1442
BisI GCNGC 7 cut(s) 77, 182, 315, 405, 648, 804, 1764
BlnI CCTAGG 1 cut(s) 113
BlsI GCNGC 7 cut(s) 78, 183, 316, 406, 649, 805, 1765
Bme1390I CCNGG 4 cut(s) 95, 194, 1189, 1738
Bme18I GGWCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
BmgT120I GGNCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
BmiI GGNNCC 9 cut(s) 189, 190, 441, 855, 856, 1314, 1397, 1466, 1668
BmrFI CCNGG 4 cut(s) 95, 194, 1189, 1738
BmsI GCATC 3 cut(s) 16, 159, 537
BmtI GCTAGC 1 cut(s) 795
BpiI GAAGAC 2 cut(s) 577, 998
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 341
BsaI GGTCTC 3 cut(s) 103, 230, 711
BsaJI CCNNGG 6 cut(s) 113, 192, 502, 1108, 1320, 1737
BsaWI WCCGGW 1 cut(s) 1750
Bsc4I CCNNNNNNNGG 3 cut(s) 503, 1321, 1322
Bse1I ACTGG 3 cut(s) 839, 1457, 1602
Bse21I CCTNAGG 1 cut(s) 1167
Bse3DI GCAATG 3 cut(s) 802, 972, 1395
BseBI CCWGG 4 cut(s) 95, 194, 1189, 1738
BseDI CCNNGG 6 cut(s) 113, 192, 502, 1108, 1320, 1737
BseGI GGATG 2 cut(s) 174, 889
BseLI CCNNNNNNNGG 3 cut(s) 503, 1321, 1322
BseMI GCAATG 3 cut(s) 802, 972, 1395
BseMII CTCAG 5 cut(s) 768, 1121, 1158, 1299, 1557
BseNI ACTGG 3 cut(s) 839, 1457, 1602
BseXI GCAGC 5 cut(s) 63, 301, 391, 634, 1750
BseYI CCCAGC 2 cut(s) 184, 428
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 3 cut(s) 181, 234, 1401
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 5 cut(s) 806, 1038, 1223, 1378, 1808
BshNI GGYRCC 2 cut(s) 1395, 1464
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 2 cut(s) 1142, 1586
BsiSI CCGG 3 cut(s) 59, 80, 1751
BslFI GGGAC 3 cut(s) 174, 840, 1072
BslI CCNNNNNNNGG 3 cut(s) 503, 1321, 1322
BsmAI GTCTC 7 cut(s) 103, 230, 711, 915, 1095, 1302, 1617
BsmBI CGTCTC 1 cut(s) 1617
BsmFI GGGAC 3 cut(s) 174, 840, 1072
BsmI GAATGC 2 cut(s) 568, 1490
BsnI GGCC 5 cut(s) 806, 1038, 1223, 1378, 1808
Bso31I GGTCTC 3 cut(s) 103, 230, 711
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 1142, 1586
Bsp143I GATC 4 cut(s) 103, 936, 1060, 1381
BspACI CCGC 8 cut(s) 181, 326, 479, 712, 803, 1183, 1399, 1720
BspANI GGCC 5 cut(s) 806, 1038, 1223, 1378, 1808
BspCNI CTCAG 5 cut(s) 769, 1122, 1159, 1300, 1558
BspFNI CGCG 3 cut(s) 181, 234, 1401
BspLI GGNNCC 9 cut(s) 189, 190, 441, 855, 856, 1314, 1397, 1466, 1668
BspMAI CTGCAG 1 cut(s) 1591
BspOI GCTAGC 1 cut(s) 795
BspT104I TTCGAA 1 cut(s) 395
BspT107I GGYRCC 2 cut(s) 1395, 1464
BspTNI GGTCTC 3 cut(s) 103, 230, 711
BsrDI GCAATG 3 cut(s) 802, 972, 1395
BsrI ACTGG 3 cut(s) 839, 1457, 1602
BssECI CCNNGG 6 cut(s) 113, 192, 502, 1108, 1320, 1737
BssMI GATC 4 cut(s) 103, 936, 1060, 1381
BssT1I CCWWGG 4 cut(s) 113, 502, 1108, 1320
Bst2UI CCWGG 4 cut(s) 95, 194, 1189, 1738
Bst4CI ACNGT 3 cut(s) 40, 739, 997
Bst6I CTCTTC 2 cut(s) 1718, 1782
BstAPI GCANNNNNTGC 1 cut(s) 1413
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 5 cut(s) 426, 793, 1023, 1040, 1405
BstDEI CTNAG 5 cut(s) 777, 1130, 1167, 1308, 1566
BstENI CCTNNNNNAGG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 174, 889
BstFNI CGCG 3 cut(s) 181, 234, 1401
BstHHI GCGC 1 cut(s) 1403
BstKTI GATC 4 cut(s) 106, 939, 1063, 1384
BstMAI GTCTC 7 cut(s) 103, 230, 711, 915, 1095, 1302, 1617
BstMBI GATC 4 cut(s) 103, 936, 1060, 1381
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 6 cut(s) 178, 323, 446, 455, 1220, 1413
BstNI CCWGG 4 cut(s) 95, 194, 1189, 1738
BstNSI RCATGY 1 cut(s) 133
BstSCI CCNGG 4 cut(s) 93, 192, 1187, 1736
BstSFI CTRYAG 1 cut(s) 1587
BstUI CGCG 3 cut(s) 181, 234, 1401
BstV1I GCAGC 5 cut(s) 63, 301, 391, 634, 1750
BstV2I GAAGAC 2 cut(s) 577, 998
BstXI CCANNNNNNTGG 1 cut(s) 866
Bsu36I CCTNAGG 1 cut(s) 1167
BsuI GTATCC 1 cut(s) 1442
BsuRI GGCC 5 cut(s) 806, 1038, 1223, 1378, 1808
BtgZI GCGATG 1 cut(s) 1039
BtsCI GGATG 2 cut(s) 174, 889
BtsIMutI CAGTG 4 cut(s) 744, 1127, 1354, 1693
Cac8I GCNNGC 5 cut(s) 426, 793, 1023, 1040, 1405
CaiI CAGNNNCTG 1 cut(s) 220
CfoI GCGC 1 cut(s) 1403
Cfr13I GGNCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
CseI GACGC 1 cut(s) 571
Csp6I GTAC 5 cut(s) 543, 552, 1276, 1396, 1705
CviAII CATG 3 cut(s) 130, 860, 1273
CviQI GTAC 5 cut(s) 543, 552, 1276, 1396, 1705
DdeI CTNAG 5 cut(s) 777, 1130, 1167, 1308, 1566
DpnI GATC 4 cut(s) 105, 938, 1062, 1383
DpnII GATC 4 cut(s) 103, 936, 1060, 1381
Eam1104I CTCTTC 2 cut(s) 1718, 1782
EarI CTCTTC 2 cut(s) 1718, 1782
EciI GGCGGA 1 cut(s) 341
Eco130I CCWWGG 4 cut(s) 113, 502, 1108, 1320
Eco147I AGGCCT 2 cut(s) 1378, 1808
Eco31I GGTCTC 3 cut(s) 103, 230, 711
Eco32I GATATC 2 cut(s) 967, 1794
Eco47I GGWCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
Eco57I CTGAAG 2 cut(s) 1221, 1452
Eco81I CCTNAGG 1 cut(s) 1167
EcoNI CCTNNNNNAGG 1 cut(s) 501
EcoO109I RGGNCCY 3 cut(s) 188, 854, 1312
EcoRI GAATTC 1 cut(s) 1634
EcoRII CCWGG 4 cut(s) 93, 192, 1187, 1736
EcoRV GATATC 2 cut(s) 967, 1794
EcoT14I CCWWGG 4 cut(s) 113, 502, 1108, 1320
ErhI CCWWGG 4 cut(s) 113, 502, 1108, 1320
Esp3I CGTCTC 1 cut(s) 1617
FaeI CATG 3 cut(s) 133, 863, 1276
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 3 cut(s) 174, 840, 1072
FatI CATG 3 cut(s) 129, 859, 1272
FauI CCCGC 2 cut(s) 486, 705
FbaI TGATCA 2 cut(s) 1060, 1381
Fnu4HI GCNGC 7 cut(s) 77, 182, 315, 405, 648, 804, 1764
FokI GGATG 2 cut(s) 181, 896
Fsp4HI GCNGC 7 cut(s) 77, 182, 315, 405, 648, 804, 1764
FspBI CTAG 4 cut(s) 114, 567, 792, 1029
GlaI GCGC 1 cut(s) 1402
GluI GCNGC 7 cut(s) 77, 182, 315, 405, 648, 804, 1764
GsaI CCCAGC 2 cut(s) 188, 432
HaeIII GGCC 5 cut(s) 806, 1038, 1223, 1378, 1808
HapII CCGG 3 cut(s) 59, 80, 1751
HgaI GACGC 1 cut(s) 571
HhaI GCGC 1 cut(s) 1403
Hin1II CATG 3 cut(s) 133, 863, 1276
Hin6I GCGC 1 cut(s) 1401
HinP1I GCGC 1 cut(s) 1401
HincII GTYRAC 2 cut(s) 600, 1125
HindII GTYRAC 2 cut(s) 600, 1125
HinfI GANTC 7 cut(s) 65, 211, 217, 1160, 1196, 1243, 1769
HpaI GTTAAC 1 cut(s) 600
HpaII CCGG 3 cut(s) 59, 80, 1751
Hpy166II GTNNAC 3 cut(s) 475, 600, 1125
Hpy188I TCNGA 8 cut(s) 103, 216, 274, 778, 1201, 1282, 1309, 1698
Hpy188III TCNNGA 3 cut(s) 815, 1157, 1446
Hpy8I GTNNAC 3 cut(s) 475, 600, 1125
Hpy99I CGWCG 1 cut(s) 1562
HpyAV CCTTC 8 cut(s) 462, 569, 924, 1196, 1260, 1417, 1454, 1798
HpyCH4III ACNGT 3 cut(s) 40, 739, 997
HpyCH4V TGCA 9 cut(s) 317, 650, 742, 1025, 1407, 1416, 1488, 1511, 1589
HpyF10VI GCNNNNNNNGC 6 cut(s) 178, 323, 446, 455, 1220, 1413
HpyF3I CTNAG 5 cut(s) 777, 1130, 1167, 1308, 1566
Hsp92II CATG 3 cut(s) 133, 863, 1276
HspAI GCGC 1 cut(s) 1401
KflI GGGWCCC 2 cut(s) 188, 854
KpnI GGTACC 1 cut(s) 1399
Ksp22I TGATCA 2 cut(s) 1060, 1381
KspAI GTTAAC 1 cut(s) 600
Kzo9I GATC 4 cut(s) 103, 936, 1060, 1381
LmnI GCTCC 3 cut(s) 445, 874, 1753
Lsp1109I GCAGC 5 cut(s) 63, 301, 391, 634, 1750
LweI GCATC 3 cut(s) 16, 159, 537
MaeI CTAG 4 cut(s) 114, 567, 792, 1029
MaeIII GTNAC 7 cut(s) 196, 289, 529, 1288, 1426, 1516, 1690
MalI GATC 4 cut(s) 105, 938, 1062, 1383
MboI GATC 4 cut(s) 103, 936, 1060, 1381
MfeI CAATTG 1 cut(s) 1043
MhlI GDGCHC 2 cut(s) 1142, 1586
MlyI GAGTC 2 cut(s) 74, 1252
MmeI TCCRAC 2 cut(s) 1078, 1663
MroXI GAANNNNTTC 1 cut(s) 1634
MseI TTAA 9 cut(s) 11, 252, 599, 639, 732, 764, 864, 1353, 1493
MslI CAYNNNNRTG 1 cut(s) 864
MspI CCGG 3 cut(s) 59, 80, 1751
MspR9I CCNGG 4 cut(s) 95, 194, 1189, 1738
MunI CAATTG 1 cut(s) 1043
Mva1269I GAATGC 2 cut(s) 568, 1490
MvaI CCWGG 4 cut(s) 95, 194, 1189, 1738
MvnI CGCG 3 cut(s) 181, 234, 1401
MwoI GCNNNNNNNGC 6 cut(s) 178, 323, 446, 455, 1220, 1413
NdeII GATC 4 cut(s) 103, 936, 1060, 1381
NheI GCTAGC 1 cut(s) 791
NlaIII CATG 3 cut(s) 133, 863, 1276
NlaIV GGNNCC 9 cut(s) 189, 190, 441, 855, 856, 1314, 1397, 1466, 1668
NmuCI GTSAC 3 cut(s) 196, 1288, 1426
NspI RCATGY 1 cut(s) 133
NspV TTCGAA 1 cut(s) 395
PceI AGGCCT 2 cut(s) 1378, 1808
PciI ACATGT 1 cut(s) 129
PctI GAATGC 2 cut(s) 568, 1490
PdmI GAANNNNTTC 1 cut(s) 1634
PfeI GAWTC 5 cut(s) 211, 217, 1160, 1196, 1769
PflFI GACNNNGTC 1 cut(s) 1286
PkrI GCNGC 7 cut(s) 78, 183, 316, 406, 649, 805, 1765
PleI GAGTC 2 cut(s) 73, 1251
PpsI GAGTC 2 cut(s) 73, 1251
PpuMI RGGWCCY 3 cut(s) 188, 854, 1312
PscI ACATGT 1 cut(s) 129
Psp5II RGGWCCY 3 cut(s) 188, 854, 1312
Psp6I CCWGG 4 cut(s) 93, 192, 1187, 1736
PspFI CCCAGC 2 cut(s) 184, 428
PspGI CCWGG 4 cut(s) 93, 192, 1187, 1736
PspN4I GGNNCC 9 cut(s) 189, 190, 441, 855, 856, 1314, 1397, 1466, 1668
PspPI GGNCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
PspPPI RGGWCCY 3 cut(s) 188, 854, 1312
PstI CTGCAG 1 cut(s) 1591
PstNI CAGNNNCTG 1 cut(s) 220
PsyI GACNNNGTC 1 cut(s) 1286
RsaI GTAC 5 cut(s) 544, 553, 1277, 1397, 1706
RsaNI GTAC 5 cut(s) 543, 552, 1276, 1396, 1705
RseI CAYNNNNRTG 1 cut(s) 864
SaqAI TTAA 9 cut(s) 11, 252, 599, 639, 732, 764, 864, 1353, 1493
SatI GCNGC 7 cut(s) 77, 182, 315, 405, 648, 804, 1764
Sau3AI GATC 4 cut(s) 103, 936, 1060, 1381
Sau96I GGNCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
SchI GAGTC 2 cut(s) 74, 1252
ScrFI CCNGG 4 cut(s) 95, 194, 1189, 1738
SduI GDGCHC 2 cut(s) 1142, 1586
SfaNI GCATC 3 cut(s) 16, 159, 537
SfcI CTRYAG 1 cut(s) 1587
SfuI TTCGAA 1 cut(s) 395
SinI GGWCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SpeI ACTAGT 1 cut(s) 1028
SseBI AGGCCT 2 cut(s) 1378, 1808
SsiI CCGC 8 cut(s) 181, 326, 479, 712, 803, 1183, 1399, 1720
SspI AATATT 1 cut(s) 1146
SspMI CTAG 4 cut(s) 114, 567, 792, 1029
StuI AGGCCT 2 cut(s) 1378, 1808
StyD4I CCNGG 4 cut(s) 93, 192, 1187, 1736
StyI CCWWGG 4 cut(s) 113, 502, 1108, 1320
TaaI ACNGT 3 cut(s) 40, 739, 997
TaqI TCGA 5 cut(s) 35, 395, 939, 1772, 1796
TatI WGTACW 2 cut(s) 1275, 1704
TauI GCSGC 2 cut(s) 184, 806
TfiI GAWTC 5 cut(s) 211, 217, 1160, 1196, 1769
Tru1I TTAA 9 cut(s) 11, 252, 599, 639, 732, 764, 864, 1353, 1493
Tru9I TTAA 9 cut(s) 11, 252, 599, 639, 732, 764, 864, 1353, 1493
TscAI CASTG 4 cut(s) 744, 1134, 1354, 1693
TseFI GTSAC 3 cut(s) 196, 1288, 1426
TseI GCWGC 5 cut(s) 76, 314, 404, 647, 1763
Tsp45I GTSAC 3 cut(s) 196, 1288, 1426
TspDTI ATGAA 9 cut(s) 621, 657, 768, 813, 1206, 1351, 1494, 1608, 1647
TspGWI ACGGA 2 cut(s) 432, 1635
TspRI CASTG 4 cut(s) 744, 1134, 1354, 1693
Tth111I GACNNNGTC 1 cut(s) 1286
VpaK11BI GGWCC 7 cut(s) 91, 188, 513, 854, 1185, 1269, 1312
XagI CCTNNNNNAGG 1 cut(s) 501
XapI RAATTY 3 cut(s) 621, 824, 1634
XceI RCATGY 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 2 cut(s) 200, 385
XmaJI CCTAGG 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 1634
XspI CTAG 4 cut(s) 114, 567, 792, 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.