AT5G61000

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009976
Physical Location & Seq
Reverse (-)
24549479 .. 24552855
3377 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61000.1

Sequence Viewer

Length: 1890 bp
ATGCAGACTTCGGTGACCCCAGATGCCATCTCCACCGTTTTGTCAAACCCATCCTTTGATTCGTCTTCCGATCGATCTGAGATCGTTGTTCAAGTTGTGGATCTTAAACCTATCGGCAATCGCTATACATTTAGTGCAAATGACGGAAAGACGAAAGTCAAAGCAATGTTTACTGCTTCACTCACACCTGAAATCATTTCTGGGAAGATCCAGAACTTGGGTCTAATTCGTCTCATTGATTTCACTGTTAACGACATTTCAAGCAAATCTACAAAATATTTCCTTGTGACTAAATGTGAGGCTGTTGGTTCTGTGCTTGATTCTGAGATTAACTTGGACAGTAAGAGCGGAGAGGAGGAGGCTCGTGAGCCCAAGAAGCAGAAACTAGAGCATTCTCCTGTCAGTCCTCTGAATGATGTTGTTTCTACTGGTATCACATTGAAACCGAAGCAGGAATTTGTAGCCAAATCAGCTTCTCAGATAATGAGTGAACAAAGGGGAAATGCTGCACCAGCTGCAAGAATGGCCATGACTAGAAGGGTTCATCCACTTGTGTCATTAAATCCATATCAAGGAAACTGGACCATCAAAGTCCGAGTTACAAACAAGGGAGTGATGAGAAATTACAAAAACGCTAGGGGTGAAGGATGTGTCTTCAATGTTGAACTAACAGATGAAGAAGGAACACAAATTCAAGCAACGATGTTTAATGATGCTGCAAGGAAGTTTTTTGACAGATTCCAGTTGGGAAAGGTTTATTATATATCAAGGGGGTCACTAAAACTCGCTAACAAGCAGTTCAAAACAGTTCAGAATGATTATGAGATGACACTGAATGAAAATTCAGAGGTTGAGGAAGCTAGTAGCGAAGAAATGTTCATCCCTGAGACAAAATTCAACTTCGTACCCATTGAAGAGTTGGGTCTGTATGTTAATCAGAAGGAACTTATTGATCTTATTGGAGTTGTTCAAAGTGTATCTCCTACCATGAGTATTAGAAGGAGAACTGACAATGAGATGATTCCTAAGAGGGATATAACTTTGGCTGATGAGTCAAGGAAAACTGTTGTGGTGTCCCTGTGGAATGATCTTGCAACTGGCATAGGTCAGGAGCTACTAGACATGGCTGATCAGTCACCTGTGATTGCAATCAAGTCTCTCAAAGTTGGAGATTTTCAAGGTGTTTCGTTGTCTACCATCAGCAGAAGCAATGTGGTTATTAACCCGGAATCACCAGAAGCTAAAAAGTTGAAATCTTGGTTTGATTCTGAAGGCAAGGAAATATCCATGTCTTCTATTGGCTCAGGGATGAGCCCTTCTGCCAAAAATGGATCTCGGTCTTTGTATACTGACCGAGTGCTTCTCTCTCACATCACAAGCAATCCATCTTTATTCGAAGAAAAGCCTGTATTTTTCAGCACTAGGGCTTACATAAGCTTCATCAAACCAGACCAAACAATGTGGTACCAAGCTTGTAAGACTTGTAACAAGAAAGTGACAGAAGCACTTGACTCTGGTTACTGGTGTGAAGGATGCCAGAGAAAGTATGAAGAATGCAGCTTAAGGTACATAATGGCGGTGAAAGTCACCGACTCATCTGGAGAAACTTGGATTTCGTCATTCAACGATGAAGCAGAGAAGATTCTTGGATGTTCAGCTGATGAGCTCAACAAACTAAAATCAGAGGAAGGTGAAGTAAATGAATATCAGACAAAACTGAAAGAGGCAACATGGTCATCTCATGTCTTCCGGGTTAGTGTCACACAGAATGAGTATAACGGTGAGAAGAGACAGAGGGTAACTGTAAAAGGAGTAGCTCCTCTTGATTTTGCTGCTGAAACAAGATTGTTGCTTCAAGACATCTCCAACAAGAACAAAACATCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

629

Amino Acids

70.26

Weight (kDa)

6.1

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 103 6.8e-26 Replication factor-A protein 1, N-terminal domain
DUF223 PF02721 182 - 286 1.3e-10 Domain of unknown function DUF223
tRNA_anti-codon PF01336 194 - 279 8.4e-10 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 311 - 408 1.8e-22 Replication protein A OB domain
Rep_fac-A_C PF08646 471 - 617 5.5e-49 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1464
AccB1I GGYRCC 1 cut(s) 1464
AccB7I CCANNNNNTGG 1 cut(s) 217
AccBSI CCGCTC 1 cut(s) 348
AccI GTMKAC 2 cut(s) 1193, 1346
AciI CCGC 2 cut(s) 348, 1577
AclWI GGATC 3 cut(s) 108, 202, 1339
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 4 cut(s) 455, 690, 841, 893
AcuI CTGAAG 1 cut(s) 1290
AfaI GTAC 3 cut(s) 906, 1466, 1568
AfiI CCNNNNNNNGG 2 cut(s) 217, 572
AflII CTTAAG 1 cut(s) 1561
Alw21I GWGCWC 1 cut(s) 1668
Alw26I GTCTC 4 cut(s) 236, 881, 1161, 1783
AlwI GGATC 3 cut(s) 108, 202, 1339
AoxI GGCC 1 cut(s) 525
ApeKI GCWGC 5 cut(s) 506, 515, 716, 1557, 1832
ApoI RAATTY 4 cut(s) 455, 690, 841, 893
Asp718I GGTACC 1 cut(s) 1464
AspS9I GGNCC 1 cut(s) 582
AsuC2I CCSGG 2 cut(s) 1226, 1751
AsuHPI GGTGA 8 cut(s) 25, 653, 1128, 1224, 1579, 1591, 1703, 1793
AsuII TTCGAA 1 cut(s) 1395
AvaII GGWCC 1 cut(s) 582
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 1 cut(s) 1464
BanII GRGCYC 3 cut(s) 372, 1316, 1668
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 4 cut(s) 57, 646, 1284, 1738
Bbv12I GWGCWC 1 cut(s) 1668
BbvI GCAGC 5 cut(s) 493, 502, 703, 1569, 1819
BccI CCATC 5 cut(s) 35, 58, 593, 1205, 1393
BclI TGATCA 1 cut(s) 1129
BcnI CCSGG 2 cut(s) 1226, 1751
BcoDI GTCTC 4 cut(s) 236, 881, 1161, 1783
BfaI CTAG 6 cut(s) 386, 534, 636, 861, 1118, 1422
BfrI CTTAAG 1 cut(s) 1561
BisI GCNGC 5 cut(s) 507, 516, 717, 1558, 1833
BlsI GCNGC 5 cut(s) 508, 517, 718, 1559, 1834
Bme1390I CCNGG 2 cut(s) 1226, 1751
Bme18I GGWCC 1 cut(s) 582
BmgT120I GGNCC 1 cut(s) 582
BmiI GGNNCC 1 cut(s) 1466
BmrFI CCNGG 2 cut(s) 1226, 1751
BmsI GCATC 3 cut(s) 13, 703, 1523
BoxI GACNNNNGTC 1 cut(s) 155
BpiI GAAGAC 4 cut(s) 57, 646, 1284, 1738
BplI GAGNNNNNCTC 2 cut(s) 1347, 1379
BpmI CTGGAG 1 cut(s) 1620
Bpu10I CCTNAGC 1 cut(s) 1303
Bpu14I TTCGAA 1 cut(s) 1395
BpuMI CCSGG 2 cut(s) 1226, 1751
Bsa29I ATCGAT 1 cut(s) 73
BsaBI GATNNNNATC 1 cut(s) 1148
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 572
Bse1I ACTGG 5 cut(s) 433, 584, 742, 1102, 1526
Bse3DI GCAATG 2 cut(s) 171, 1216
Bse8I GATNNNNATC 1 cut(s) 1148
BseCI ATCGAT 1 cut(s) 73
BseGI GGATG 7 cut(s) 50, 544, 653, 879, 1314, 1538, 1655
BseJI GATNNNNATC 1 cut(s) 1148
BseLI CCNNNNNNNGG 2 cut(s) 217, 572
BseMI GCAATG 2 cut(s) 171, 1216
BseMII CTCAG 5 cut(s) 69, 315, 491, 876, 1317
BseNI ACTGG 5 cut(s) 433, 584, 742, 1102, 1526
BseRI GAGGAG 3 cut(s) 368, 371, 1809
BseXI GCAGC 5 cut(s) 493, 502, 703, 1569, 1819
BsgI GTGCAG 1 cut(s) 492
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 1 cut(s) 527
BshNI GGYRCC 1 cut(s) 1464
BshVI ATCGAT 1 cut(s) 73
BsiEI CGRYCG 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 1668
BsiSI CCGG 2 cut(s) 1226, 1750
BslFI GGGAC 1 cut(s) 1060
BslI CCNNNNNNNGG 2 cut(s) 217, 572
BsmAI GTCTC 4 cut(s) 236, 881, 1161, 1783
BsmBI CGTCTC 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 1060
BsmI GAATGC 2 cut(s) 391, 1559
BsnI GGCC 1 cut(s) 527
Bsp119I TTCGAA 1 cut(s) 1395
Bsp1286I GDGCHC 3 cut(s) 372, 1316, 1668
Bsp143I GATC 9 cut(s) 70, 74, 81, 100, 207, 952, 1087, 1129, 1331
BspACI CCGC 2 cut(s) 348, 1577
BspANI GGCC 1 cut(s) 527
BspCNI CTCAG 5 cut(s) 70, 316, 490, 877, 1316
BspDI ATCGAT 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 1466
BspPI GGATC 3 cut(s) 108, 202, 1339
BspT104I TTCGAA 1 cut(s) 1395
BspT107I GGYRCC 1 cut(s) 1464
BspTI CTTAAG 1 cut(s) 1561
BsrBI CCGCTC 1 cut(s) 348
BsrDI GCAATG 2 cut(s) 171, 1216
BsrI ACTGG 5 cut(s) 433, 584, 742, 1102, 1526
BssMI GATC 9 cut(s) 70, 74, 81, 100, 207, 952, 1087, 1129, 1331
BssNAI GTATAC 1 cut(s) 1347
BssSI CACGAG 1 cut(s) 363
Bst1107I GTATAC 1 cut(s) 1347
Bst2BI CACGAG 1 cut(s) 363
Bst4CI ACNGT 7 cut(s) 37, 247, 341, 808, 1066, 1781, 1804
Bst6I CTCTTC 2 cut(s) 909, 1781
BstAFI CTTAAG 1 cut(s) 1561
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 1395
BstDEI CTNAG 7 cut(s) 78, 324, 477, 885, 1026, 1303, 1884
BstEII GGTNACC 1 cut(s) 13
BstF5I GGATG 7 cut(s) 50, 544, 653, 879, 1314, 1538, 1655
BstKTI GATC 9 cut(s) 73, 77, 84, 103, 210, 955, 1090, 1132, 1334
BstMAI GTCTC 4 cut(s) 236, 881, 1161, 1783
BstMBI GATC 9 cut(s) 70, 74, 81, 100, 207, 952, 1087, 1129, 1331
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 5 cut(s) 376, 470, 512, 515, 524
BstPAI GACNNNNGTC 1 cut(s) 155
BstPI GGTNACC 1 cut(s) 13
BstSCI CCNGG 2 cut(s) 1224, 1749
BstV1I GCAGC 5 cut(s) 493, 502, 703, 1569, 1819
BstV2I GAAGAC 4 cut(s) 57, 646, 1284, 1738
BstX2I RGATCY 3 cut(s) 100, 207, 1331
BstYI RGATCY 3 cut(s) 100, 207, 1331
BstZ17I GTATAC 1 cut(s) 1347
Bsu15I ATCGAT 1 cut(s) 73
BsuRI GGCC 1 cut(s) 527
BsuTUI ATCGAT 1 cut(s) 73
BtsCI GGATG 7 cut(s) 50, 544, 653, 879, 1314, 1538, 1655
BtsIMutI CAGTG 2 cut(s) 243, 830
Cfr13I GGNCC 1 cut(s) 582
ClaI ATCGAT 1 cut(s) 73
Csp6I GTAC 3 cut(s) 905, 1465, 1567
CspCI CAANNNNNGTGG 2 cut(s) 1442, 1477
CviAII CATG 6 cut(s) 529, 988, 1123, 1288, 1731, 1742
CviQI GTAC 3 cut(s) 905, 1465, 1567
DdeI CTNAG 7 cut(s) 78, 324, 477, 885, 1026, 1303, 1884
DpnI GATC 9 cut(s) 72, 76, 83, 102, 209, 954, 1089, 1131, 1333
DpnII GATC 9 cut(s) 70, 74, 81, 100, 207, 952, 1087, 1129, 1331
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 2 cut(s) 909, 1781
EarI CTCTTC 2 cut(s) 909, 1781
Ecl136II GAGCTC 1 cut(s) 1666
Eco24I GRGCYC 3 cut(s) 372, 1316, 1668
Eco47I GGWCC 1 cut(s) 582
Eco53kI GAGCTC 1 cut(s) 1666
Eco57I CTGAAG 1 cut(s) 1290
Eco91I GGTNACC 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 1666
EcoO65I GGTNACC 1 cut(s) 13
EcoT38I GRGCYC 3 cut(s) 372, 1316, 1668
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 6 cut(s) 532, 991, 1126, 1291, 1734, 1745
FaqI GGGAC 1 cut(s) 1060
FatI CATG 6 cut(s) 528, 987, 1122, 1287, 1730, 1741
FbaI TGATCA 1 cut(s) 1129
FblI GTMKAC 2 cut(s) 1193, 1346
Fnu4HI GCNGC 5 cut(s) 507, 516, 717, 1558, 1833
FokI GGATG 7 cut(s) 37, 531, 660, 866, 1321, 1545, 1662
FriOI GRGCYC 3 cut(s) 372, 1316, 1668
Fsp4HI GCNGC 5 cut(s) 507, 516, 717, 1558, 1833
FspBI CTAG 6 cut(s) 386, 534, 636, 861, 1118, 1422
GluI GCNGC 5 cut(s) 507, 516, 717, 1558, 1833
GsuI CTGGAG 1 cut(s) 1620
HaeIII GGCC 1 cut(s) 527
HapII CCGG 2 cut(s) 1226, 1750
Hin1II CATG 6 cut(s) 532, 991, 1126, 1291, 1734, 1745
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 2 cut(s) 1435, 1470
HpaI GTTAAC 1 cut(s) 250
HpaII CCGG 2 cut(s) 1226, 1750
HphI GGTGA 8 cut(s) 25, 653, 1128, 1224, 1579, 1591, 1703, 1793
Hpy166II GTNNAC 5 cut(s) 171, 250, 491, 1194, 1347
Hpy188III TCNNGA 6 cut(s) 211, 365, 1109, 1599, 1823, 1856
Hpy8I GTNNAC 5 cut(s) 171, 250, 491, 1194, 1347
HpyAV CCTTC 9 cut(s) 531, 638, 674, 934, 993, 1265, 1326, 1523, 1682
HpyCH4III ACNGT 7 cut(s) 37, 247, 341, 808, 1066, 1781, 1804
HpyCH4V TGCA 8 cut(s) 4, 137, 509, 518, 719, 1094, 1148, 1557
HpyF10VI GCNNNNNNNGC 5 cut(s) 376, 470, 512, 515, 524
HpyF3I CTNAG 7 cut(s) 78, 324, 477, 885, 1026, 1303, 1884
Hsp92II CATG 6 cut(s) 532, 991, 1126, 1291, 1734, 1745
KpnI GGTACC 1 cut(s) 1468
Ksp22I TGATCA 1 cut(s) 1129
KspAI GTTAAC 1 cut(s) 250
Kzo9I GATC 9 cut(s) 70, 74, 81, 100, 207, 952, 1087, 1129, 1331
LmnI GCTCC 2 cut(s) 1111, 1822
Lsp1109I GCAGC 5 cut(s) 493, 502, 703, 1569, 1819
LweI GCATC 3 cut(s) 13, 703, 1523
MaeI CTAG 6 cut(s) 386, 534, 636, 861, 1118, 1422
MalI GATC 9 cut(s) 72, 76, 83, 102, 209, 954, 1089, 1131, 1333
MbiI CCGCTC 1 cut(s) 348
MboI GATC 9 cut(s) 70, 74, 81, 100, 207, 952, 1087, 1129, 1331
MflI RGATCY 3 cut(s) 100, 207, 1331
MhlI GDGCHC 3 cut(s) 372, 1316, 1668
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 6 cut(s) 225, 455, 622, 690, 841, 893
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 3 cut(s) 1061, 1505, 1586
MmeI TCCRAC 2 cut(s) 1147, 1890
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MseI TTAA 8 cut(s) 105, 249, 330, 560, 708, 933, 1221, 1562
Msp20I TGGCCA 1 cut(s) 527
MspA1I CMGCKG 2 cut(s) 515, 1658
MspCI CTTAAG 1 cut(s) 1561
MspI CCGG 2 cut(s) 1226, 1750
MspR9I CCNGG 2 cut(s) 1226, 1751
Mva1269I GAATGC 2 cut(s) 391, 1559
MwoI GCNNNNNNNGC 5 cut(s) 376, 470, 512, 515, 524
NciI CCSGG 2 cut(s) 1226, 1751
NdeII GATC 9 cut(s) 70, 74, 81, 100, 207, 952, 1087, 1129, 1331
NlaIII CATG 6 cut(s) 532, 991, 1126, 1291, 1734, 1745
NlaIV GGNNCC 1 cut(s) 1466
NmuCI GTSAC 7 cut(s) 13, 286, 774, 1134, 1495, 1585, 1759
NspV TTCGAA 1 cut(s) 1395
PctI GAATGC 2 cut(s) 391, 1559
PfeI GAWTC 7 cut(s) 59, 320, 738, 1021, 1229, 1265, 1642
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 5 cut(s) 508, 517, 718, 1559, 1834
Ple19I CGATCG 1 cut(s) 73
PleI GAGTC 3 cut(s) 1060, 1505, 1586
PpsI GAGTC 3 cut(s) 1060, 1505, 1586
PshAI GACNNNNGTC 1 cut(s) 155
Psp124BI GAGCTC 1 cut(s) 1668
PspEI GGTNACC 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 1466
PspPI GGNCC 1 cut(s) 582
PsuI RGATCY 3 cut(s) 100, 207, 1331
PvuI CGATCG 1 cut(s) 73
PvuII CAGCTG 2 cut(s) 515, 1658
RsaI GTAC 3 cut(s) 906, 1466, 1568
RsaNI GTAC 3 cut(s) 905, 1465, 1567
SacI GAGCTC 1 cut(s) 1668
SaqAI TTAA 8 cut(s) 105, 249, 330, 560, 708, 933, 1221, 1562
SatI GCNGC 5 cut(s) 507, 516, 717, 1558, 1833
Sau3AI GATC 9 cut(s) 70, 74, 81, 100, 207, 952, 1087, 1129, 1331
Sau96I GGNCC 1 cut(s) 582
SchI GAGTC 3 cut(s) 1061, 1505, 1586
ScrFI CCNGG 2 cut(s) 1226, 1751
SduI GDGCHC 3 cut(s) 372, 1316, 1668
SfaNI GCATC 3 cut(s) 13, 703, 1523
SfuI TTCGAA 1 cut(s) 1395
SinI GGWCC 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 1561
SmoI CTYRAG 1 cut(s) 1561
Sse9I AATT 6 cut(s) 225, 455, 622, 690, 841, 893
SsiI CCGC 2 cut(s) 348, 1577
SspI AATATT 1 cut(s) 278
SspMI CTAG 6 cut(s) 386, 534, 636, 861, 1118, 1422
SstI GAGCTC 1 cut(s) 1668
StyD4I CCNGG 2 cut(s) 1224, 1749
TaaI ACNGT 7 cut(s) 37, 247, 341, 808, 1066, 1781, 1804
TaqI TCGA 2 cut(s) 73, 1395
TaqII GACCGA 2 cut(s) 1326, 1368
TasI AATT 6 cut(s) 225, 455, 622, 690, 841, 893
TfiI GAWTC 7 cut(s) 59, 320, 738, 1021, 1229, 1265, 1642
Tru1I TTAA 8 cut(s) 105, 249, 330, 560, 708, 933, 1221, 1562
Tru9I TTAA 8 cut(s) 105, 249, 330, 560, 708, 933, 1221, 1562
TscAI CASTG 2 cut(s) 250, 837
TseFI GTSAC 7 cut(s) 13, 286, 774, 1134, 1495, 1585, 1759
TseI GCWGC 5 cut(s) 506, 515, 716, 1557, 1832
Tsp45I GTSAC 7 cut(s) 13, 286, 774, 1134, 1495, 1585, 1759
TspDTI ATGAA 8 cut(s) 533, 690, 852, 868, 1429, 1563, 1644, 1716
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 2 cut(s) 250, 837
Van91I CCANNNNNTGG 1 cut(s) 217
Vha464I CTTAAG 1 cut(s) 1561
VpaK11BI GGWCC 1 cut(s) 582
XapI RAATTY 4 cut(s) 455, 690, 841, 893
XcmI CCANNNNNNNNNTGG 1 cut(s) 916
XmiI GTMKAC 2 cut(s) 1193, 1346
XspI CTAG 6 cut(s) 386, 534, 636, 861, 1118, 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.