AT5G09650

Soluble inorganic pyrophosphatase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009636
Physical Location & Seq
Reverse (-)
2991149 .. 2993213
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G09650.1

Sequence Viewer

Length: 903 bp
ATGGCGGCTACTAGAGTGTTAACTGCAGCGACGGCCGTGACCCAAACCACCTCCTGTTTCCTCGCCAAGCAAGCTTTCACTCTTCCGGCGAAGAAATCCTGCGGCGGATTTGGTGGTCTCTGCTTCAGCAGAAGAGCTTTGGTGTTGAAATCTAAGAGACCTTTCTCCTGCAGTGCCATTTACAATCCTCAGGTTAAGGTTCAAGAAGAAGGTCCGGCCGAATCCCTAGATTATCGAGTTTTCTTCCTCGATGGTTCTGGAAAGAAGGTTTCTCCATGGCATGATATACCATTGACCTTAGGCGATGGAGTTTTCAACTTTATAGTTGAAATACCTAAAGAGTCAAAAGCAAAAATGGAGGTTGCTACTGATGAAGATTTCACTCCTATTAAGCAGGATACTAAGAAGGGGAAGCTCAGATATTATCCGTATAACATTAACTGGAACTATGGGTTGCTTCCTCAAACATGGGAAGATCCATCTCATGCAAATTCTGAAGTTGAAGGATGTTTTGGGGATAATGATCCAGTTGATGTTGTTGAGATTGGTGAAACACAAAGGAAGATAGGCGATATTCTAAAGATAAAGCCTTTAGCTGCTTTAGCTATGATTGATGAAGGTGAGCTAGACTGGAAGATTGTTGCCATTTCTTTGGATGACCCAAAAGCTCATCTTGTGAATGATGTTGAAGATGTTGAGAAACATTTCCCGGGTACACTAACAGCCATTAGAGACTGGTTTAGAGACTACAAGATCCCAGATGGAAAGCCTGCTAACAGATTTGGTCTTGGAGACAAACCAGCAAACAAAGACTATGCTTTGAAGATCATCCAAGAAACAAATGAATCATGGGCTAAACTTGTGAAGAGATCAGTTGATGCTGGAGACCTTTCACTTTACTGA

Protein Analysis

300

Amino Acids

33.38

Weight (kDa)

5.72

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 107 - 284 1.9e-40 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 102, 105
AclWI GGATC 3 cut(s) 470, 518, 748
AcoI YGGCCR 2 cut(s) 33, 216
AcsI RAATTY 1 cut(s) 490
AcuI CTGAAG 2 cut(s) 109, 516
AfaI GTAC 1 cut(s) 715
AgsI TTSAA 7 cut(s) 148, 203, 316, 329, 503, 689, 823
AjuI GAANNNNNNNTTGG 4 cut(s) 59, 91, 495, 527
AluBI AGCT 7 cut(s) 74, 137, 415, 596, 605, 625, 668
AluI AGCT 7 cut(s) 74, 137, 415, 596, 605, 625, 668
Alw26I GTCTC 6 cut(s) 122, 151, 726, 738, 786, 879
AlwI GGATC 3 cut(s) 470, 518, 748
Ama87I CYCGRG 1 cut(s) 709
AoxI GGCC 2 cut(s) 33, 216
ApeKI GCWGC 2 cut(s) 26, 596
ApoI RAATTY 1 cut(s) 490
Asp700I GAANNNNTTC 1 cut(s) 704
AspS9I GGNCC 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 710, 711
AsuHPI GGTGA 2 cut(s) 560, 632
AvaI CYCGRG 1 cut(s) 709
AvaII GGWCC 1 cut(s) 212
AxyI CCTNAGG 2 cut(s) 189, 298
BbvI GCAGC 2 cut(s) 38, 583
BccI CCATC 4 cut(s) 245, 299, 487, 755
BceAI ACGGC 2 cut(s) 20, 48
BciVI GTATCC 1 cut(s) 391
BcnI CCSGG 2 cut(s) 710, 711
BcoDI GTCTC 6 cut(s) 122, 151, 726, 738, 786, 879
BfaI CTAG 3 cut(s) 12, 227, 626
BfmI CTRYAG 2 cut(s) 24, 169
BfuI GTATCC 1 cut(s) 391
BisI GCNGC 4 cut(s) 6, 27, 103, 597
BlsI GCNGC 4 cut(s) 7, 28, 104, 598
Bme1390I CCNGG 2 cut(s) 710, 711
Bme18I GGWCC 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 709
BmgT120I GGNCC 1 cut(s) 212
BmrFI CCNGG 2 cut(s) 710, 711
BmsI GCATC 1 cut(s) 868
BpmI CTGGAG 1 cut(s) 903
BpuMI CCSGG 2 cut(s) 710, 711
BsaBI GATNNNNATC 1 cut(s) 522
BsaI GGTCTC 3 cut(s) 122, 151, 879
BsaJI CCNNGG 2 cut(s) 275, 709
Bse1I ACTGG 4 cut(s) 446, 527, 635, 740
Bse21I CCTNAGG 2 cut(s) 189, 298
Bse8I GATNNNNATC 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 275, 709
BseGI GGATG 3 cut(s) 512, 661, 828
BseJI GATNNNNATC 1 cut(s) 522
BseMII CTCAG 2 cut(s) 203, 430
BseNI ACTGG 4 cut(s) 446, 527, 635, 740
BseX3I CGGCCG 2 cut(s) 33, 216
BseXI GCAGC 2 cut(s) 38, 583
Bsh1285I CGRYCG 2 cut(s) 36, 219
BshFI GGCC 2 cut(s) 35, 218
BsiEI CGRYCG 2 cut(s) 36, 219
BsiHKCI CYCGRG 1 cut(s) 709
BsiSI CCGG 3 cut(s) 86, 215, 710
BsmAI GTCTC 6 cut(s) 122, 151, 726, 738, 786, 879
BsnI GGCC 2 cut(s) 35, 218
Bso31I GGTCTC 3 cut(s) 122, 151, 879
BsoBI CYCGRG 1 cut(s) 709
Bsp143I GATC 5 cut(s) 475, 523, 753, 825, 869
Bsp19I CCATGG 1 cut(s) 275
BspACI CCGC 3 cut(s) 5, 102, 105
BspANI GGCC 2 cut(s) 35, 218
BspCNI CTCAG 2 cut(s) 202, 429
BspMAI CTGCAG 2 cut(s) 28, 173
BspPI GGATC 3 cut(s) 470, 518, 748
BspQI GCTCTTC 1 cut(s) 127
BspTNI GGTCTC 3 cut(s) 122, 151, 879
BsrI ACTGG 4 cut(s) 446, 527, 635, 740
BssECI CCNNGG 2 cut(s) 275, 709
BssMI GATC 5 cut(s) 475, 523, 753, 825, 869
BssT1I CCWWGG 1 cut(s) 275
Bst6I CTCTTC 3 cut(s) 87, 127, 860
BstC8I GCNNGC 2 cut(s) 72, 771
BstDEI CTNAG 5 cut(s) 153, 189, 298, 402, 416
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 3 cut(s) 512, 661, 828
BstKTI GATC 5 cut(s) 478, 526, 756, 828, 872
BstMAI GTCTC 6 cut(s) 122, 151, 726, 738, 786, 879
BstMBI GATC 5 cut(s) 475, 523, 753, 825, 869
BstMCI CGRYCG 2 cut(s) 36, 219
BstMWI GCNNNNNNNGC 3 cut(s) 32, 71, 602
BstSCI CCNGG 2 cut(s) 708, 709
BstSFI CTRYAG 2 cut(s) 24, 169
BstV1I GCAGC 2 cut(s) 38, 583
BstX2I RGATCY 2 cut(s) 475, 753
BstXI CCANNNNNNTGG 1 cut(s) 652
BstYI RGATCY 2 cut(s) 475, 753
BstZI CGGCCG 2 cut(s) 33, 216
Bsu36I CCTNAGG 2 cut(s) 189, 298
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 2 cut(s) 35, 218
BtgI CCRYGG 1 cut(s) 275
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 3 cut(s) 512, 661, 828
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 1 cut(s) 178
Cac8I GCNNGC 2 cut(s) 72, 771
Cfr13I GGNCC 1 cut(s) 212
Cfr9I CCCGGG 1 cut(s) 709
Csp6I GTAC 1 cut(s) 714
CviAII CATG 5 cut(s) 276, 281, 468, 485, 849
CviQI GTAC 1 cut(s) 714
DdeI CTNAG 5 cut(s) 153, 189, 298, 402, 416
DpnI GATC 5 cut(s) 477, 525, 755, 827, 871
DpnII GATC 5 cut(s) 475, 523, 753, 825, 869
EaeI YGGCCR 2 cut(s) 33, 216
EagI CGGCCG 2 cut(s) 33, 216
Eam1104I CTCTTC 3 cut(s) 87, 127, 860
EarI CTCTTC 3 cut(s) 87, 127, 860
EciI GGCGGA 1 cut(s) 120
EclXI CGGCCG 2 cut(s) 33, 216
Eco130I CCWWGG 1 cut(s) 275
Eco31I GGTCTC 3 cut(s) 122, 151, 879
Eco47I GGWCC 1 cut(s) 212
Eco52I CGGCCG 2 cut(s) 33, 216
Eco57I CTGAAG 2 cut(s) 109, 516
Eco81I CCTNAGG 2 cut(s) 189, 298
Eco88I CYCGRG 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 5 cut(s) 279, 284, 471, 488, 852
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FatI CATG 5 cut(s) 275, 280, 467, 484, 848
Fnu4HI GCNGC 4 cut(s) 6, 27, 103, 597
FokI GGATG 3 cut(s) 519, 668, 815
Fsp4HI GCNGC 4 cut(s) 6, 27, 103, 597
FspBI CTAG 3 cut(s) 12, 227, 626
GluI GCNGC 4 cut(s) 6, 27, 103, 597
GsuI CTGGAG 1 cut(s) 903
HaeIII GGCC 2 cut(s) 35, 218
HapII CCGG 3 cut(s) 86, 215, 710
Hin1II CATG 5 cut(s) 279, 284, 471, 488, 852
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HindIII AAGCTT 1 cut(s) 72
HinfI GANTC 3 cut(s) 221, 341, 845
HpaI GTTAAC 1 cut(s) 21
HpaII CCGG 3 cut(s) 86, 215, 710
HphI GGTGA 2 cut(s) 560, 632
Hpy166II GTNNAC 2 cut(s) 21, 716
Hpy188I TCNGA 2 cut(s) 419, 496
Hpy188III TCNNGA 2 cut(s) 203, 258
Hpy8I GTNNAC 2 cut(s) 21, 716
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 5 cut(s) 203, 259, 400, 497, 611
HpyCH4V TGCA 3 cut(s) 26, 171, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 71, 602
HpyF3I CTNAG 5 cut(s) 153, 189, 298, 402, 416
Hsp92II CATG 5 cut(s) 279, 284, 471, 488, 852
KspAI GTTAAC 1 cut(s) 21
Kzo9I GATC 5 cut(s) 475, 523, 753, 825, 869
LguI GCTCTTC 1 cut(s) 127
Lsp1109I GCAGC 2 cut(s) 38, 583
LweI GCATC 1 cut(s) 868
MaeI CTAG 3 cut(s) 12, 227, 626
MaeIII GTNAC 1 cut(s) 37
MalI GATC 5 cut(s) 477, 525, 755, 827, 871
MboI GATC 5 cut(s) 475, 523, 753, 825, 869
MflI RGATCY 2 cut(s) 475, 753
MluCI AATT 1 cut(s) 490
MlyI GAGTC 1 cut(s) 350
MnlI CCTC 6 cut(s) 61, 71, 198, 257, 352, 471
MroXI GAANNNNTTC 1 cut(s) 704
MseI TTAA 4 cut(s) 20, 195, 390, 438
MspI CCGG 3 cut(s) 86, 215, 710
MspR9I CCNGG 2 cut(s) 710, 711
MwoI GCNNNNNNNGC 3 cut(s) 32, 71, 602
NciI CCSGG 2 cut(s) 710, 711
NcoI CCATGG 1 cut(s) 275
NdeII GATC 5 cut(s) 475, 523, 753, 825, 869
NlaIII CATG 5 cut(s) 279, 284, 471, 488, 852
NmuCI GTSAC 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 704
PfeI GAWTC 2 cut(s) 221, 845
PkrI GCNGC 4 cut(s) 7, 28, 104, 598
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PspPI GGNCC 1 cut(s) 212
PstI CTGCAG 2 cut(s) 28, 173
PsuI RGATCY 2 cut(s) 475, 753
RsaI GTAC 1 cut(s) 715
RsaNI GTAC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 127
SaqAI TTAA 4 cut(s) 20, 195, 390, 438
SatI GCNGC 4 cut(s) 6, 27, 103, 597
Sau3AI GATC 5 cut(s) 475, 523, 753, 825, 869
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 2 cut(s) 710, 711
SfaNI GCATC 1 cut(s) 868
SfcI CTRYAG 2 cut(s) 24, 169
SinI GGWCC 1 cut(s) 212
SmaI CCCGGG 1 cut(s) 711
Sse9I AATT 1 cut(s) 490
SsiI CCGC 3 cut(s) 5, 102, 105
SspMI CTAG 3 cut(s) 12, 227, 626
StyD4I CCNGG 2 cut(s) 708, 709
StyI CCWWGG 1 cut(s) 275
TaqI TCGA 2 cut(s) 235, 249
TasI AATT 1 cut(s) 490
TauI GCSGC 2 cut(s) 8, 105
TfiI GAWTC 2 cut(s) 221, 845
Tru1I TTAA 4 cut(s) 20, 195, 390, 438
Tru9I TTAA 4 cut(s) 20, 195, 390, 438
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 2 cut(s) 26, 596
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 387, 630, 858
TspGWI ACGGA 1 cut(s) 417
TspMI CCCGGG 1 cut(s) 709
TspRI CASTG 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 490
XmaI CCCGGG 1 cut(s) 709
XmnI GAANNNNTTC 1 cut(s) 704
XspI CTAG 3 cut(s) 12, 227, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.