MD17G1239600.v1.1

Soluble inorganic pyrophosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009636
Physical Location & Seq
Forward (+)
28896055 .. 28900422
4368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1239600.v1.1.491

Sequence Viewer

Length: 891 bp
ATGGCCACCGCGAGGGTGTTGACTGTCTCCAATACTTCCGGCTTACTCTCCAAAACGACGTCGTTCCCGGTGAAGCAGAGTCCCTACGGCACCAGCCTTTGCTTTACTAGAAGGAGAGTAGCATCATTGTCGTCTTCCTCCAGGAGGTCCTTCAATTGCACCGCTATCTACAAACCCGATGTTCAGATCAGGGAGGAAGGCCAGCCTGAAACCCTAGACTACCGGCTCTTCTTCGTCGATCGCTCTGGTCAAAAGGTTTCGCCTTGGCACGACATACCTTTGCAAGTGGGTGATGGTGCATTCAACTTTGTGGTTGAAATACCCAAAGAATCAAGTGCAAAGATGGAGGTTGCTACTGATGAGCCACACACCCCGATAAAGCAGGATACAAAGAAGGGGAAACTTCGTTACTACCCGTACAATATAAATTGGAATTATGGGTTGCTTCCGCAAACATGGGAAGACCCAACCCTTGCTAACGCTGAAGTTGAAGGAGCCTTTGGGGATAATGATCCTGTTGATGTTGTTGAGATAGGCGATAGTCGGAGAAGGATTGGCGAGATTCTCAAAGTAAAGCCTTTGGCTGCTTTAGCTATGATTGATGAAGGGGAGCTTGATTGGAAAATTATTGCAATTTCGTTGGATGATCCAAGGGCTTCTCTCGTTAATGACATTGATGATGTTGAGAAGCATTTCCCGGGGACTCTCACTGCAATAAGGGACTGGTTTAGAGACTACAAGATACCAGATGGAAAGCCTGCTAACAAGTTTGGTCTTGGCAACAAAGCAGCAAACAAGGACTATGCTCTTAAGGTCATAACCGAAACTAATGAATCATGGGCCAAGCTTGTCAAGAGATCGATTCCTGCCGGAGAACTGTCACTTGCATAG

Protein Analysis

297

Amino Acids

33.04

Weight (kDa)

6.03

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 103 - 280 1.1e-41 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 89
AccII CGCG 1 cut(s) 11
AciI CCGC 3 cut(s) 9, 162, 449
AclWI GGATC 2 cut(s) 506, 641
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 504
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 2 cut(s) 12, 144
AflII CTTAAG 1 cut(s) 809
AgsI TTSAA 4 cut(s) 154, 304, 317, 491
AjnI CCWGG 1 cut(s) 140
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
AluBI AGCT 3 cut(s) 593, 613, 847
AluI AGCT 3 cut(s) 593, 613, 847
Alw26I GTCTC 2 cut(s) 31, 726
AlwI GGATC 2 cut(s) 506, 641
Ama87I CYCGRG 1 cut(s) 697
AoxI GGCC 3 cut(s) 3, 199, 840
ApeKI GCWGC 2 cut(s) 584, 788
Asp700I GAANNNNTTC 1 cut(s) 692
AspS9I GGNCC 2 cut(s) 147, 840
AsuC2I CCSGG 3 cut(s) 68, 698, 699
AsuHPI GGTGA 2 cut(s) 82, 302
AvaI CYCGRG 1 cut(s) 697
AvaII GGWCC 1 cut(s) 147
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 89
BbsI GAAGAC 2 cut(s) 126, 468
BbvI GCAGC 2 cut(s) 571, 800
BccI CCATC 3 cut(s) 287, 337, 743
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 111, 145
BciT130I CCWGG 1 cut(s) 142
BciVI GTATCC 1 cut(s) 379
BcnI CCSGG 3 cut(s) 68, 698, 699
BcoDI GTCTC 2 cut(s) 31, 726
BfaI CTAG 2 cut(s) 108, 215
BfrI CTTAAG 1 cut(s) 809
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 2 cut(s) 585, 789
BlsI GCNGC 2 cut(s) 586, 790
Bme1390I CCNGG 4 cut(s) 68, 142, 698, 699
Bme18I GGWCC 1 cut(s) 147
BmeT110I CYCGRG 1 cut(s) 697
BmgT120I GGNCC 2 cut(s) 147, 840
BmiI GGNNCC 2 cut(s) 91, 496
BmrFI CCNGG 4 cut(s) 68, 142, 698, 699
BmsI GCATC 1 cut(s) 131
BpiI GAAGAC 2 cut(s) 126, 468
BpmI CTGGAG 1 cut(s) 124
BpuMI CCSGG 3 cut(s) 68, 698, 699
Bsa29I ATCGAT 1 cut(s) 860
BsaBI GATNNNNATC 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 4 cut(s) 263, 650, 697, 698
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 144
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 1 cut(s) 728
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 142
BseCI ATCGAT 1 cut(s) 860
BseDI CCNNGG 4 cut(s) 263, 650, 697, 698
BseGI GGATG 1 cut(s) 649
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 12, 144
BseNI ACTGG 1 cut(s) 728
BseXI GCAGC 2 cut(s) 571, 800
Bsh1236I CGCG 1 cut(s) 11
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 3 cut(s) 5, 201, 842
BshNI GGYRCC 1 cut(s) 89
BshVI ATCGAT 1 cut(s) 860
BsiEI CGRYCG 1 cut(s) 241
BsiHKCI CYCGRG 1 cut(s) 697
BsiSI CCGG 5 cut(s) 39, 68, 223, 698, 870
BslFI GGGAC 3 cut(s) 66, 715, 734
BslI CCNNNNNNNGG 2 cut(s) 12, 144
BsmAI GTCTC 2 cut(s) 31, 726
BsmFI GGGAC 3 cut(s) 66, 715, 734
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 3 cut(s) 5, 201, 842
BsoBI CYCGRG 1 cut(s) 697
Bsp143I GATC 5 cut(s) 186, 238, 511, 646, 857
BspACI CCGC 3 cut(s) 9, 162, 449
BspANI GGCC 3 cut(s) 5, 201, 842
BspDI ATCGAT 1 cut(s) 860
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 2 cut(s) 91, 496
BspPI GGATC 2 cut(s) 506, 641
BspQI GCTCTTC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 809
BsrFI RCCGGY 1 cut(s) 222
BsrI ACTGG 1 cut(s) 728
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 4 cut(s) 263, 650, 697, 698
BssMI GATC 5 cut(s) 186, 238, 511, 646, 857
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 2 cut(s) 263, 650
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 25, 879
Bst6I CTCTTC 1 cut(s) 233
BstACI GRCGYC 1 cut(s) 59
BstAFI CTTAAG 1 cut(s) 809
BstC8I GCNNGC 2 cut(s) 203, 759
BstENI CCTNNNNNAGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 649
BstFNI CGCG 1 cut(s) 11
BstKTI GATC 5 cut(s) 189, 241, 514, 649, 860
BstMAI GTCTC 2 cut(s) 31, 726
BstMBI GATC 5 cut(s) 186, 238, 511, 646, 857
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 1 cut(s) 590
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 4 cut(s) 66, 140, 696, 697
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 571, 800
BstV2I GAAGAC 2 cut(s) 126, 468
Bsu15I ATCGAT 1 cut(s) 860
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 3 cut(s) 5, 201, 842
BsuTUI ATCGAT 1 cut(s) 860
BtsCI GGATG 1 cut(s) 649
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 1 cut(s) 708
Cac8I GCNNGC 2 cut(s) 203, 759
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 147, 840
Cfr9I CCCGGG 1 cut(s) 697
ClaI ATCGAT 1 cut(s) 860
Csp6I GTAC 1 cut(s) 418
CviAII CATG 2 cut(s) 456, 837
CviQI GTAC 1 cut(s) 418
DpnI GATC 5 cut(s) 188, 240, 513, 648, 859
DpnII GATC 5 cut(s) 186, 238, 511, 646, 857
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco130I CCWWGG 2 cut(s) 263, 650
Eco47I GGWCC 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 697
EcoNI CCTNNNNNAGG 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 2 cut(s) 263, 650
ErhI CCWWGG 2 cut(s) 263, 650
FaeI CATG 2 cut(s) 459, 840
FaiI YATR 9 cut(s) 275, 425, 438, 457, 596, 804, 818, 838, 889
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 3 cut(s) 66, 715, 734
FatI CATG 2 cut(s) 455, 836
Fnu4HI GCNGC 2 cut(s) 585, 789
FokI GGATG 1 cut(s) 656
Fsp4HI GCNGC 2 cut(s) 585, 789
FspBI CTAG 2 cut(s) 108, 215
GluI GCNGC 2 cut(s) 585, 789
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 3 cut(s) 5, 201, 842
HapII CCGG 5 cut(s) 39, 68, 223, 698, 870
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 2 cut(s) 459, 840
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 6 cut(s) 79, 329, 562, 703, 833, 862
HpaII CCGG 5 cut(s) 39, 68, 223, 698, 870
HphI GGTGA 2 cut(s) 82, 302
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 2 cut(s) 186, 546
Hpy188III TCNNGA 1 cut(s) 853
Hpy8I GTNNAC 1 cut(s) 21
Hpy99I CGWCG 3 cut(s) 61, 64, 239
HpyAV CCTTC 7 cut(s) 105, 160, 191, 388, 485, 543, 599
HpyCH4III ACNGT 2 cut(s) 25, 879
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 7 cut(s) 159, 283, 299, 338, 632, 713, 887
HpyF10VI GCNNNNNNNGC 1 cut(s) 590
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 2 cut(s) 459, 840
Kzo9I GATC 5 cut(s) 186, 238, 511, 646, 857
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 2 cut(s) 494, 610
Lsp1109I GCAGC 2 cut(s) 571, 800
LweI GCATC 1 cut(s) 131
MaeI CTAG 2 cut(s) 108, 215
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 2 cut(s) 407, 879
MalI GATC 5 cut(s) 188, 240, 513, 648, 859
MboI GATC 5 cut(s) 186, 238, 511, 646, 857
MboII GAAGA 4 cut(s) 126, 220, 223, 473
MfeI CAATTG 1 cut(s) 154
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 154, 427, 433, 624, 633
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 88, 697
MmeI TCCRAC 2 cut(s) 524, 621
MnlI CCTC 5 cut(s) 6, 138, 148, 187, 340
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 692
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 666, 810
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 809
MspI CCGG 5 cut(s) 39, 68, 223, 698, 870
MspR9I CCNGG 4 cut(s) 68, 142, 698, 699
MunI CAATTG 1 cut(s) 154
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 590
NciI CCSGG 3 cut(s) 68, 698, 699
NdeII GATC 5 cut(s) 186, 238, 511, 646, 857
NlaIII CATG 2 cut(s) 459, 840
NlaIV GGNNCC 2 cut(s) 91, 496
NmuCI GTSAC 1 cut(s) 879
PciSI GCTCTTC 1 cut(s) 233
PctI GAATGC 1 cut(s) 299
PdmI GAANNNNTTC 1 cut(s) 692
PfeI GAWTC 4 cut(s) 329, 562, 833, 862
PfoI TCCNGGA 1 cut(s) 140
PkrI GCNGC 2 cut(s) 586, 790
Ple19I CGATCG 1 cut(s) 241
PleI GAGTC 2 cut(s) 87, 697
PpsI GAGTC 2 cut(s) 87, 697
PpuMI RGGWCCY 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 147
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 91, 496
PspPI GGNCC 2 cut(s) 147, 840
PspPPI RGGWCCY 1 cut(s) 147
PvuI CGATCG 1 cut(s) 241
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 2 cut(s) 666, 810
SatI GCNGC 2 cut(s) 585, 789
Sau3AI GATC 5 cut(s) 186, 238, 511, 646, 857
Sau96I GGNCC 2 cut(s) 147, 840
SchI GAGTC 2 cut(s) 88, 697
ScrFI CCNGG 4 cut(s) 68, 142, 698, 699
SetI ASST 9 cut(s) 62, 149, 258, 280, 351, 595, 615, 816, 849
SfaNI GCATC 1 cut(s) 131
SinI GGWCC 1 cut(s) 147
SmaI CCCGGG 1 cut(s) 699
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
Sse9I AATT 5 cut(s) 154, 427, 433, 624, 633
SsiI CCGC 3 cut(s) 9, 162, 449
SspMI CTAG 2 cut(s) 108, 215
StyD4I CCNGG 4 cut(s) 66, 140, 696, 697
StyI CCWWGG 2 cut(s) 263, 650
TaaI ACNGT 2 cut(s) 25, 879
TaiI ACGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 237, 860
TasI AATT 5 cut(s) 154, 427, 433, 624, 633
TfiI GAWTC 4 cut(s) 329, 562, 833, 862
Tru1I TTAA 2 cut(s) 666, 810
Tru9I TTAA 2 cut(s) 666, 810
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 1 cut(s) 879
TseI GCWGC 2 cut(s) 584, 788
Tsp45I GTSAC 1 cut(s) 879
TspDTI ATGAA 2 cut(s) 618, 846
TspMI CCCGGG 1 cut(s) 697
TspRI CASTG 1 cut(s) 715
Vha464I CTTAAG 1 cut(s) 809
VpaK11BI GGWCC 1 cut(s) 147
XagI CCTNNNNNAGG 1 cut(s) 142
XmaI CCCGGG 1 cut(s) 697
XmnI GAANNNNTTC 1 cut(s) 692
XspI CTAG 2 cut(s) 108, 215
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.