MD10G1058400.v1.1

Soluble inorganic pyrophosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009636
Physical Location & Seq
Reverse (-)
7886788 .. 7898949
12162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1058400.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGCCACCGCGAGAGTGCTGACCGCCGCCAATACTTCCTGCTTGCTCTCCAAGGCGACGTCGTTCCTTCCGAAGCAGAGTCCCTACAGCACTACCACCCTTTGCTTCACTAGAAGAAGAGCAGCGTCGCTGTCGTTGTCGTCTTCGTCCAGGAGCAGGAGGTCCTTCATTTGCACAGCTATCTACAATCCCGAGGTTCAGATCAAGGAGGAAGGCCAGCCGGAAACCCTAGACTACCGCCTCTTCTTCGTCGACCGTTCCGGTCAAAAGGTTTCGCCTTGGCATGATATACCGTTGCAAGTGGGTGATGGTGTATTCAACGTTGTGGTTGAAATACCCAAAGAATCGAGTGCAAAGATGGAGGTTGCTACTGATGAGCCACACACCCCAATAAAGCAGGATACAAAGAAAGGAAAACTTCGTTACTATCCCTACAACATTAATTGGAATTATGGGTTGCTTCCACAAACGTGGGAAGACCCATCGCTTGCTAACAATGAAGTTGGAGGAGCATTTGGGGATAATGATCCAGTTGATGTTGTTGAGATAGGTGATAGTCGGAGAAAGATTGGTGAGATTCTCAAAGTAAAGCCATTGGCTGCTTTAGCTATGATCGATGAAGGGGAACTTGACTGGAAAATTGTTGCAATTTCATTGGATGATCCAAGGGCTTCTCTAGTTAACGACGTTGATGATGTTGACAAGCATTTCCCAGGGACTCTCACTGCAATAAGGGACTGGTTCAGAGACTACAAGATACCTGATGGAAAGCCTGCTAACAAATTTGGTCTTGGCAACAAAGCAGCAAACAAGGATTACGCTCTTAAGGTCATATCCGAAACCAATGAATCGTGGGCAAAGCTTGTCAAGAGATCAATTCCTGCTGGAGAACTATCACTTGCATAG

Protein Analysis

302

Amino Acids

33.41

Weight (kDa)

6.36

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 108 - 285 2.4e-41 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 252
AccII CGCG 1 cut(s) 11
AciI CCGC 4 cut(s) 9, 24, 27, 238
AclI AACGTT 1 cut(s) 321
AclWI GGATC 2 cut(s) 521, 656
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 782
AcyI GRCGYC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 156
AflII CTTAAG 1 cut(s) 824
AgsI TTSAA 2 cut(s) 319, 332
AjnI CCWGG 2 cut(s) 149, 712
AleI CACNNNNGTG 1 cut(s) 469
AluBI AGCT 3 cut(s) 179, 608, 862
AluI AGCT 3 cut(s) 179, 608, 862
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 2 cut(s) 521, 656
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 2 cut(s) 3, 214
ApeKI GCWGC 3 cut(s) 122, 599, 803
ApoI RAATTY 1 cut(s) 782
AseI ATTAAT 1 cut(s) 441
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 3 cut(s) 317, 563, 584
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 162
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 135, 483
BbvI GCAGC 3 cut(s) 134, 586, 815
BccI CCATC 4 cut(s) 302, 352, 490, 758
BciT130I CCWGG 2 cut(s) 151, 714
BciVI GTATCC 1 cut(s) 394
BcoDI GTCTC 1 cut(s) 741
BfaI CTAG 3 cut(s) 111, 230, 677
BfmI CTRYAG 1 cut(s) 85
BfrI CTTAAG 1 cut(s) 824
BfuI GTATCC 1 cut(s) 394
BisI GCNGC 4 cut(s) 27, 123, 600, 804
BlsI GCNGC 4 cut(s) 28, 124, 601, 805
Bme1390I CCNGG 2 cut(s) 151, 714
Bme18I GGWCC 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 162
BmrFI CCNGG 2 cut(s) 151, 714
BpiI GAAGAC 2 cut(s) 135, 483
BpmI CTGGAG 1 cut(s) 906
Bsa29I ATCGAT 1 cut(s) 615
BsaBI GATNNNNATC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 6 cut(s) 51, 192, 278, 665, 712, 713
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 3 cut(s) 530, 638, 743
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 2 cut(s) 151, 714
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 6 cut(s) 51, 192, 278, 665, 712, 713
BseGI GGATG 1 cut(s) 664
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 1 cut(s) 156
BseNI ACTGG 3 cut(s) 530, 638, 743
BseRI GAGGAG 1 cut(s) 522
BseXI GCAGC 3 cut(s) 134, 586, 815
Bsh1236I CGCG 1 cut(s) 11
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 2 cut(s) 5, 216
BshVI ATCGAT 1 cut(s) 615
BsiEI CGRYCG 1 cut(s) 256
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 2 cut(s) 221, 261
BslFI GGGAC 3 cut(s) 66, 730, 749
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 741
BsmFI GGGAC 3 cut(s) 66, 730, 749
BsnI GGCC 2 cut(s) 5, 216
BsoBI CYCGRG 1 cut(s) 191
Bsp143I GATC 5 cut(s) 201, 526, 612, 661, 872
BspACI CCGC 4 cut(s) 9, 24, 27, 238
BspANI GGCC 2 cut(s) 5, 216
BspDI ATCGAT 1 cut(s) 615
BspFNI CGCG 1 cut(s) 11
BspPI GGATC 2 cut(s) 521, 656
BspQI GCTCTTC 1 cut(s) 112
BspTI CTTAAG 1 cut(s) 824
BsrI ACTGG 3 cut(s) 530, 638, 743
BssECI CCNNGG 6 cut(s) 51, 192, 278, 665, 712, 713
BssMI GATC 5 cut(s) 201, 526, 612, 661, 872
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 3 cut(s) 51, 278, 665
Bst2UI CCWGG 2 cut(s) 151, 714
Bst4CI ACNGT 2 cut(s) 257, 294
Bst6I CTCTTC 2 cut(s) 112, 248
BstACI GRCGYC 1 cut(s) 59
BstAFI CTTAAG 1 cut(s) 824
BstC8I GCNNGC 4 cut(s) 44, 218, 489, 774
BstF5I GGATG 1 cut(s) 664
BstFNI CGCG 1 cut(s) 11
BstKTI GATC 5 cut(s) 204, 529, 615, 664, 875
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 5 cut(s) 201, 526, 612, 661, 872
BstMCI CGRYCG 1 cut(s) 256
BstMWI GCNNNNNNNGC 1 cut(s) 605
BstNI CCWGG 2 cut(s) 151, 714
BstSCI CCNGG 2 cut(s) 149, 712
BstSFI CTRYAG 1 cut(s) 85
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 3 cut(s) 134, 586, 815
BstV2I GAAGAC 2 cut(s) 135, 483
BstXI CCANNNNNNTGG 1 cut(s) 471
Bsu15I ATCGAT 1 cut(s) 615
BsuI GTATCC 1 cut(s) 394
BsuRI GGCC 2 cut(s) 5, 216
BsuTUI ATCGAT 1 cut(s) 615
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 1 cut(s) 664
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 1 cut(s) 723
Cac8I GCNNGC 4 cut(s) 44, 218, 489, 774
Cfr13I GGNCC 1 cut(s) 162
ClaI ATCGAT 1 cut(s) 615
CseI GACGC 1 cut(s) 114
CviAII CATG 1 cut(s) 284
DpnI GATC 5 cut(s) 203, 528, 614, 663, 874
DpnII GATC 5 cut(s) 201, 526, 612, 661, 872
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 112, 248
EarI CTCTTC 2 cut(s) 112, 248
Eco130I CCWWGG 3 cut(s) 51, 278, 665
Eco47I GGWCC 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 149, 712
EcoT14I CCWWGG 3 cut(s) 51, 278, 665
ErhI CCWWGG 3 cut(s) 51, 278, 665
FaeI CATG 1 cut(s) 287
FaiI YATR 6 cut(s) 285, 290, 453, 611, 833, 904
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 612, 644
FaqI GGGAC 3 cut(s) 66, 730, 749
FatI CATG 1 cut(s) 283
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 27, 123, 600, 804
FokI GGATG 1 cut(s) 671
Fsp4HI GCNGC 4 cut(s) 27, 123, 600, 804
FspBI CTAG 3 cut(s) 111, 230, 677
GluI GCNGC 4 cut(s) 27, 123, 600, 804
GsuI CTGGAG 1 cut(s) 906
HaeIII GGCC 2 cut(s) 5, 216
HapII CCGG 2 cut(s) 221, 261
HgaI GACGC 1 cut(s) 114
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 1 cut(s) 287
HincII GTYRAC 3 cut(s) 253, 682, 700
HindII GTYRAC 3 cut(s) 253, 682, 700
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 5 cut(s) 79, 344, 577, 718, 848
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 2 cut(s) 221, 261
HphI GGTGA 3 cut(s) 317, 563, 584
Hpy166II GTNNAC 3 cut(s) 253, 682, 700
Hpy188I TCNGA 5 cut(s) 72, 201, 561, 746, 838
Hpy188III TCNNGA 2 cut(s) 191, 868
Hpy8I GTNNAC 3 cut(s) 253, 682, 700
Hpy99I CGWCG 5 cut(s) 61, 64, 130, 254, 689
HpyAV CCTTC 4 cut(s) 77, 175, 206, 614
HpyCH4III ACNGT 2 cut(s) 257, 294
HpyCH4IV ACGT 4 cut(s) 59, 321, 470, 687
HpyCH4V TGCA 6 cut(s) 174, 298, 353, 647, 728, 902
HpyF10VI GCNNNNNNNGC 1 cut(s) 605
HpySE526I ACGT 4 cut(s) 59, 321, 470, 687
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 1 cut(s) 287
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 201, 526, 612, 661, 872
LguI GCTCTTC 1 cut(s) 112
LmnI GCTCC 2 cut(s) 153, 509
Lsp1109I GCAGC 3 cut(s) 134, 586, 815
MaeI CTAG 3 cut(s) 111, 230, 677
MaeII ACGT 4 cut(s) 59, 321, 470, 687
MaeIII GTNAC 1 cut(s) 422
MalI GATC 5 cut(s) 203, 528, 614, 663, 874
MboI GATC 5 cut(s) 201, 526, 612, 661, 872
MboII GAAGA 6 cut(s) 126, 129, 135, 235, 238, 488
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 442, 448, 639, 648, 782, 876
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 88, 712
MmeI TCCRAC 2 cut(s) 484, 539
MnlI CCTC 6 cut(s) 153, 187, 202, 251, 355, 500
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 441, 681, 825
MslI CAYNNNNRTG 1 cut(s) 469
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 824
MspI CCGG 2 cut(s) 221, 261
MspR9I CCNGG 2 cut(s) 151, 714
MvaI CCWGG 2 cut(s) 151, 714
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 605
NdeII GATC 5 cut(s) 201, 526, 612, 661, 872
NlaIII CATG 1 cut(s) 287
OliI CACNNNNGTG 1 cut(s) 469
PasI CCCWGGG 1 cut(s) 713
PciSI GCTCTTC 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PfeI GAWTC 3 cut(s) 344, 577, 848
PfoI TCCNGGA 1 cut(s) 149
PkrI GCNGC 4 cut(s) 28, 124, 601, 805
PleI GAGTC 2 cut(s) 87, 712
PpsI GAGTC 2 cut(s) 87, 712
PpuMI RGGWCCY 1 cut(s) 162
PshBI ATTAAT 1 cut(s) 441
Psp1406I AACGTT 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 149, 712
PspGI CCWGG 2 cut(s) 149, 712
PspPI GGNCC 1 cut(s) 162
PspPPI RGGWCCY 1 cut(s) 162
RseI CAYNNNNRTG 1 cut(s) 469
SalI GTCGAC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 112
SaqAI TTAA 3 cut(s) 441, 681, 825
SatI GCNGC 4 cut(s) 27, 123, 600, 804
Sau3AI GATC 5 cut(s) 201, 526, 612, 661, 872
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 2 cut(s) 88, 712
ScrFI CCNGG 2 cut(s) 151, 714
SfcI CTRYAG 1 cut(s) 85
SinI GGWCC 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 469
SmlI CTYRAG 1 cut(s) 824
SmoI CTYRAG 1 cut(s) 824
Sse9I AATT 6 cut(s) 442, 448, 639, 648, 782, 876
SsiI CCGC 4 cut(s) 9, 24, 27, 238
SspMI CTAG 3 cut(s) 111, 230, 677
StyD4I CCNGG 2 cut(s) 149, 712
StyI CCWWGG 3 cut(s) 51, 278, 665
TaaI ACNGT 2 cut(s) 257, 294
TaiI ACGT 4 cut(s) 62, 324, 473, 690
TaqI TCGA 3 cut(s) 252, 347, 615
TasI AATT 6 cut(s) 442, 448, 639, 648, 782, 876
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 3 cut(s) 344, 577, 848
Tru1I TTAA 3 cut(s) 441, 681, 825
Tru9I TTAA 3 cut(s) 441, 681, 825
TscAI CASTG 1 cut(s) 730
TseI GCWGC 3 cut(s) 122, 599, 803
TspDTI ATGAA 5 cut(s) 157, 513, 633, 642, 861
TspRI CASTG 1 cut(s) 730
Vha464I CTTAAG 1 cut(s) 824
VpaK11BI GGWCC 1 cut(s) 162
VspI ATTAAT 1 cut(s) 441
XapI RAATTY 1 cut(s) 782
XmiI GTMKAC 1 cut(s) 252
XspI CTAG 3 cut(s) 111, 230, 677
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.