AT5G09680

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005826
Physical Location & Seq
Reverse (-)
2999195 .. 3000658
1464 bp
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UTR
Exon/CDS
Intron
AT5G09680.2

Sequence Viewer

Length: 636 bp
ATGGATACTAGTAGAGATGATGATTTCACATTCTCAAAGGTTTCACCACCAGATAGTGAAGTTAAAGATCTTGCATCAGATGTAGGTAGTATTACCCTTAAAGATGGGTTAGATCAACAAAAAAGCAATGGTCTTATTTGGAAAGACAAGTCTCTTCCTCCAAAGGAAGAAACAATAGGTTCTTTGTCTTTCACGGTGACTGATTCTTCGTCTTCGAAAAAGCAATCCAATGAATCCTCTGAGACTTTCAAAACTCCAGCTAGAAAGCCTATCACTCGTACCAAGGTTCCATTTGAAAAGGGTTATAGCCAAATGGATTGGTTGAAACTTACACGAACACATCCCGATCTTGCAGGCCTGAAGGGAGAGTCAAACAAGAGGCTTATTCCAATGGATGAAGTAAAGAAACACCGAACAGGAGATTCGATGTGGACTGTTTTGAAAGGTCGTGTATACAACATATCGCCTTATATGAATTTTCATCCTGGAGGTGTCGATATGCTGATGAAAGCGGTTGGAAGAGACGGTACGCTCTTATTCAACAAATACCATGCCTGGGTCAATGTTGATATCTTACTTGAGAAATGCCTTGTTGGAGTTTTGGATGATACCAAAGTGAAGAAGCAAGAACCCTAA

Protein Analysis

211

Amino Acids

23.7

Weight (kDa)

8.78

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 131 - 202 4.9e-22 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 453
AciI CCGC 1 cut(s) 512
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 380
AfaI GTAC 2 cut(s) 280, 529
AfiI CCNNNNNNNGG 1 cut(s) 556
AgsI TTSAA 5 cut(s) 250, 296, 325, 442, 541
AhlI ACTAGT 1 cut(s) 8
AjnI CCWGG 2 cut(s) 484, 554
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw26I GTCTC 3 cut(s) 156, 236, 516
AoxI GGCC 1 cut(s) 355
ApoI RAATTY 1 cut(s) 475
ArsI GACNNNNNNTTYG 2 cut(s) 190, 222
AsuHPI GGTGA 2 cut(s) 36, 208
AsuII TTCGAA 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 600, 633
BarI GAAGNNNNNNTAC 2 cut(s) 511, 543
BbsI GAAGAC 1 cut(s) 204
BccI CCATC 1 cut(s) 98
BciT130I CCWGG 2 cut(s) 486, 556
BcoDI GTCTC 3 cut(s) 156, 236, 516
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 2 cut(s) 9, 261
BglII AGATCT 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 486, 556
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 2 cut(s) 486, 556
BmsI GCATC 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 2 cut(s) 240, 507
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 599
BsaJI CCNNGG 2 cut(s) 282, 555
Bsc4I CCNNNNNNNGG 1 cut(s) 556
Bse3DI GCAATG 1 cut(s) 133
BseBI CCWGG 2 cut(s) 486, 556
BseDI CCNNGG 2 cut(s) 282, 555
BseGI GGATG 4 cut(s) 340, 400, 481, 610
BseLI CCNNNNNNNGG 1 cut(s) 556
BseMI GCAATG 1 cut(s) 133
BseMII CTCAG 1 cut(s) 231
BshFI GGCC 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 556
BsmAI GTCTC 3 cut(s) 156, 236, 516
BsmBI CGTCTC 1 cut(s) 516
BsnI GGCC 1 cut(s) 357
Bsp119I TTCGAA 1 cut(s) 215
Bsp143I GATC 3 cut(s) 67, 112, 346
BspACI CCGC 1 cut(s) 512
BspANI GGCC 1 cut(s) 357
BspCNI CTCAG 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 288
BspT104I TTCGAA 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 282, 555
BssMI GATC 3 cut(s) 67, 112, 346
BssNAI GTATAC 1 cut(s) 454
BssT1I CCWWGG 1 cut(s) 282
Bst1107I GTATAC 1 cut(s) 454
Bst2UI CCWGG 2 cut(s) 486, 556
Bst4CI ACNGT 3 cut(s) 196, 436, 527
Bst6I CTCTTC 2 cut(s) 159, 514
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 1 cut(s) 240
BstF5I GGATG 4 cut(s) 340, 400, 481, 610
BstKTI GATC 3 cut(s) 70, 115, 349
BstMAI GTCTC 3 cut(s) 156, 236, 516
BstMBI GATC 3 cut(s) 67, 112, 346
BstNI CCWGG 2 cut(s) 486, 556
BstSCI CCNGG 2 cut(s) 484, 554
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 67
BstYI RGATCY 1 cut(s) 67
BstZ17I GTATAC 1 cut(s) 454
BsuRI GGCC 1 cut(s) 357
BtsCI GGATG 4 cut(s) 340, 400, 481, 610
Cac8I GCNNGC 1 cut(s) 355
Csp6I GTAC 2 cut(s) 279, 528
CviAII CATG 1 cut(s) 551
CviJI RGCY 5 cut(s) 260, 268, 309, 357, 382
CviKI_1 RGCY 5 cut(s) 260, 268, 309, 357, 382
CviQI GTAC 2 cut(s) 279, 528
DdeI CTNAG 1 cut(s) 240
DpnI GATC 3 cut(s) 69, 114, 348
DpnII GATC 3 cut(s) 67, 112, 346
Eam1104I CTCTTC 2 cut(s) 159, 514
EarI CTCTTC 2 cut(s) 159, 514
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 357
Eco32I GATATC 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 484, 554
EcoRV GATATC 1 cut(s) 571
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 1 cut(s) 554
FaiI YATR 7 cut(s) 306, 454, 461, 471, 473, 500, 552
FatI CATG 1 cut(s) 550
FblI GTMKAC 1 cut(s) 453
FokI GGATG 4 cut(s) 327, 407, 468, 617
FspBI CTAG 2 cut(s) 9, 261
GsuI CTGGAG 2 cut(s) 240, 507
HaeIII GGCC 1 cut(s) 357
Hin1II CATG 1 cut(s) 554
HinfI GANTC 4 cut(s) 203, 233, 368, 422
HphI GGTGA 2 cut(s) 36, 208
Hpy166II GTNNAC 2 cut(s) 432, 454
Hpy188I TCNGA 2 cut(s) 79, 241
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 2 cut(s) 432, 454
HpyAV CCTTC 1 cut(s) 355
HpyCH4III ACNGT 3 cut(s) 196, 436, 527
HpyCH4V TGCA 2 cut(s) 74, 353
HpyF3I CTNAG 1 cut(s) 240
Hsp92II CATG 1 cut(s) 554
Kzo9I GATC 3 cut(s) 67, 112, 346
LpnPI CCDG 9 cut(s) 63, 270, 339, 371, 402, 471, 498, 541, 568
LweI GCATC 1 cut(s) 83
MaeI CTAG 2 cut(s) 9, 261
MaeIII GTNAC 1 cut(s) 196
MalI GATC 3 cut(s) 69, 114, 348
MboI GATC 3 cut(s) 67, 112, 346
MboII GAAGA 6 cut(s) 146, 179, 198, 204, 531, 631
MflI RGATCY 1 cut(s) 67
MluCI AATT 1 cut(s) 475
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 2 cut(s) 496, 574
MnlI CCTC 4 cut(s) 168, 247, 372, 482
MseI TTAA 2 cut(s) 63, 99
MspR9I CCNGG 2 cut(s) 486, 556
MvaI CCWGG 2 cut(s) 486, 556
NdeII GATC 3 cut(s) 67, 112, 346
NlaIII CATG 1 cut(s) 554
NlaIV GGNNCC 1 cut(s) 288
NmuCI GTSAC 1 cut(s) 196
NspV TTCGAA 1 cut(s) 215
PceI AGGCCT 1 cut(s) 357
PfeI GAWTC 3 cut(s) 203, 233, 422
PfoI TCCNGGA 1 cut(s) 484
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp6I CCWGG 2 cut(s) 484, 554
PspGI CCWGG 2 cut(s) 484, 554
PspN4I GGNNCC 1 cut(s) 288
PsuI RGATCY 1 cut(s) 67
RsaI GTAC 2 cut(s) 280, 529
RsaNI GTAC 2 cut(s) 279, 528
SaqAI TTAA 2 cut(s) 63, 99
Sau3AI GATC 3 cut(s) 67, 112, 346
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 486, 556
SetI ASST 7 cut(s) 42, 88, 181, 262, 288, 448, 493
SfaNI GCATC 1 cut(s) 83
SfuI TTCGAA 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SpeI ACTAGT 1 cut(s) 8
Sse9I AATT 1 cut(s) 475
SseBI AGGCCT 1 cut(s) 357
SsiI CCGC 1 cut(s) 512
SspMI CTAG 2 cut(s) 9, 261
StuI AGGCCT 1 cut(s) 357
StyD4I CCNGG 2 cut(s) 484, 554
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 3 cut(s) 196, 436, 527
TaqI TCGA 3 cut(s) 215, 425, 495
TasI AATT 1 cut(s) 475
TfiI GAWTC 3 cut(s) 203, 233, 422
Tru1I TTAA 2 cut(s) 63, 99
Tru9I TTAA 2 cut(s) 63, 99
TseFI GTSAC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 5 cut(s) 246, 411, 470, 488, 521
XapI RAATTY 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 453
XspI CTAG 2 cut(s) 9, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.