Rroxscaffold_4G00330700

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005826
Physical Location & Seq
Reverse (-)
64414800 .. 64419483
4684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330700.1

Sequence Viewer

Length: 678 bp
ATGGACGCTGATAACGATTTCACGTTTTGCCAGGTTGGCTCACCTGGAAACCAGGATGCTCTCGAAACAAAGAAGCTTGCTTCAGATATAGACGCTATTAACATAAATGATGAATTGTCGAATGGCACTATTAGCAATCAGAATAGTGGTTCAATGTGGAAAGGGGGACTGCCAAGGGATGCCACCTCTGAAAAACAGAAAACAGTTGGATCTTTGTCTGTCACTGTTATTGATGCGTCATCCACAAACGAATACTCACAATTTCCAAAAAAAGTACCATCAGGAGATGGTGGAAAGTCAGTGAAGCCTGCACCTCGTGCCAAAGTTCCTTTTGAGAAGGGGTATAGCCAAATGGATTGGCTTAAGCTTACTCGAACACATCCCGACCTTGCAGGTTTGAAGGGACAGTCAAATAAGAGGCTTATTTCCATAAATGAAGTTAAACAGCACCAAAAAGGGGATTCTATGTGGACTGTTCTAAAAGGCCGTGTGTACAATTTATCTCCATACATGAGATTTCACCCTGGAGGTGTTGATATTCTAAAGAAGGCTGTGGGGAAAGACTGCACTTCTTTATTCAATAAATACCATGCTTGGGTCAATGCTGATTTTTTACTTGAGAAGTGTCTCGTTGGTACTTTAGATGATGGACAAGGGCAGCAAAATTCCAGTCACTAA

Protein Analysis

225

Amino Acids

24.61

Weight (kDa)

8.64

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 145 - 215 7.1e-23 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 383
AclWI GGATC 1 cut(s) 217
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 1 cut(s) 66
AdeI CACNNNGTG 1 cut(s) 317
AfaI GTAC 3 cut(s) 276, 494, 637
AfiI CCNNNNNNNGG 2 cut(s) 457, 595
AflII CTTAAG 1 cut(s) 362
AgsI TTSAA 3 cut(s) 153, 400, 580
AjnI CCWGG 4 cut(s) 30, 43, 51, 523
AluBI AGCT 2 cut(s) 76, 367
AluI AGCT 2 cut(s) 76, 367
Alw26I GTCTC 1 cut(s) 632
AlwI GGATC 1 cut(s) 217
AoxI GGCC 1 cut(s) 484
ApeKI GCWGC 1 cut(s) 658
ApoI RAATTY 1 cut(s) 664
ArsI GACNNNNNNTTYG 1 cut(s) 655
AsuHPI GGTGA 2 cut(s) 33, 512
BauI CACGAG 1 cut(s) 315
BbvI GCAGC 1 cut(s) 670
BccI CCATC 3 cut(s) 281, 286, 641
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 4 cut(s) 32, 45, 53, 525
BcoDI GTCTC 1 cut(s) 632
BfrI CTTAAG 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 383
BglI GCCNNNNNGGC 1 cut(s) 36
BisI GCNGC 1 cut(s) 659
BlsI GCNGC 1 cut(s) 660
Bme1390I CCNGG 4 cut(s) 32, 45, 53, 525
BmrFI CCNGG 4 cut(s) 32, 45, 53, 525
BmsI GCATC 3 cut(s) 46, 169, 223
BpmI CTGGAG 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 638
BsaJI CCNNGG 2 cut(s) 173, 523
Bsc4I CCNNNNNNNGG 2 cut(s) 457, 595
Bse1I ACTGG 1 cut(s) 669
BseBI CCWGG 4 cut(s) 32, 45, 53, 525
BseDI CCNNGG 2 cut(s) 173, 523
BseGI GGATG 4 cut(s) 61, 184, 239, 379
BseLI CCNNNNNNNGG 2 cut(s) 457, 595
BseNI ACTGG 1 cut(s) 669
BseXI GCAGC 1 cut(s) 670
BsgI GTGCAG 2 cut(s) 294, 550
BshFI GGCC 1 cut(s) 486
BslFI GGGAC 2 cut(s) 180, 417
BslI CCNNNNNNNGG 2 cut(s) 457, 595
BsmAI GTCTC 1 cut(s) 632
BsmFI GGGAC 2 cut(s) 180, 417
BsnI GGCC 1 cut(s) 486
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 1 cut(s) 209
BspANI GGCC 1 cut(s) 486
BspMI ACCTGC 1 cut(s) 383
BspPI GGATC 1 cut(s) 217
BspTI CTTAAG 1 cut(s) 362
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 1 cut(s) 669
BssECI CCNNGG 2 cut(s) 173, 523
BssMI GATC 1 cut(s) 209
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 173
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 4 cut(s) 32, 45, 53, 525
Bst4CI ACNGT 4 cut(s) 205, 226, 408, 475
BstAFI CTTAAG 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 78, 309
BstF5I GGATG 4 cut(s) 61, 184, 239, 379
BstKTI GATC 1 cut(s) 212
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 1 cut(s) 209
BstMWI GCNNNNNNNGC 3 cut(s) 36, 132, 317
BstNI CCWGG 4 cut(s) 32, 45, 53, 525
BstSCI CCNGG 4 cut(s) 30, 43, 51, 523
BstV1I GCAGC 1 cut(s) 670
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
BsuRI GGCC 1 cut(s) 486
BtsCI GGATG 4 cut(s) 61, 184, 239, 379
BtsIMutI CAGTG 2 cut(s) 222, 306
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 2 cut(s) 78, 309
CseI GACGC 3 cut(s) 14, 101, 225
Csp6I GTAC 3 cut(s) 275, 493, 636
CviAII CATG 2 cut(s) 511, 590
CviJI RGCY 9 cut(s) 39, 76, 307, 348, 361, 367, 421, 486, 551
CviKI_1 RGCY 9 cut(s) 39, 76, 307, 348, 361, 367, 421, 486, 551
CviQI GTAC 3 cut(s) 275, 493, 636
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
DraIII CACNNNGTG 1 cut(s) 317
Eco130I CCWWGG 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 66
EcoRII CCWGG 4 cut(s) 30, 43, 51, 523
EcoT14I CCWWGG 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 2 cut(s) 514, 593
FaiI YATR 8 cut(s) 89, 104, 345, 431, 467, 508, 512, 591
FaqI GGGAC 2 cut(s) 180, 417
FatI CATG 2 cut(s) 510, 589
Fnu4HI GCNGC 1 cut(s) 659
FokI GGATG 4 cut(s) 68, 191, 226, 366
Fsp4HI GCNGC 1 cut(s) 659
GluI GCNGC 1 cut(s) 659
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 1 cut(s) 486
HgaI GACGC 3 cut(s) 14, 101, 225
Hin1II CATG 2 cut(s) 514, 593
HindIII AAGCTT 2 cut(s) 74, 365
HinfI GANTC 1 cut(s) 461
HphI GGTGA 2 cut(s) 33, 512
Hpy166II GTNNAC 2 cut(s) 471, 493
Hpy188I TCNGA 3 cut(s) 85, 141, 190
Hpy188III TCNNGA 3 cut(s) 62, 282, 383
Hpy8I GTNNAC 2 cut(s) 471, 493
HpyAV CCTTC 3 cut(s) 331, 394, 541
HpyCH4III ACNGT 4 cut(s) 205, 226, 408, 475
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 311, 392, 567
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 132, 317
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 514, 593
Kzo9I GATC 1 cut(s) 209
Lsp1109I GCAGC 1 cut(s) 670
LweI GCATC 3 cut(s) 46, 169, 223
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 220, 671
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MflI RGATCY 1 cut(s) 209
MluCI AATT 4 cut(s) 113, 260, 496, 664
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 4 cut(s) 196, 324, 411, 521
MseI TTAA 3 cut(s) 99, 363, 441
MspCI CTTAAG 1 cut(s) 362
MspR9I CCNGG 4 cut(s) 32, 45, 53, 525
MvaI CCWGG 4 cut(s) 32, 45, 53, 525
MwoI GCNNNNNNNGC 3 cut(s) 36, 132, 317
NdeII GATC 1 cut(s) 209
NlaIII CATG 2 cut(s) 514, 593
NmuCI GTSAC 2 cut(s) 220, 671
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PfeI GAWTC 1 cut(s) 461
PkrI GCNGC 1 cut(s) 660
Psp6I CCWGG 4 cut(s) 30, 43, 51, 523
PspGI CCWGG 4 cut(s) 30, 43, 51, 523
PsuI RGATCY 1 cut(s) 209
RsaI GTAC 3 cut(s) 276, 494, 637
RsaNI GTAC 3 cut(s) 275, 493, 636
SaqAI TTAA 3 cut(s) 99, 363, 441
SatI GCNGC 1 cut(s) 659
Sau3AI GATC 1 cut(s) 209
ScrFI CCNGG 4 cut(s) 32, 45, 53, 525
SfaNI GCATC 3 cut(s) 46, 169, 223
SmlI CTYRAG 2 cut(s) 362, 617
SmoI CTYRAG 2 cut(s) 362, 617
Sse9I AATT 4 cut(s) 113, 260, 496, 664
StyD4I CCNGG 4 cut(s) 30, 43, 51, 523
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 4 cut(s) 205, 226, 408, 475
TaiI ACGT 1 cut(s) 26
TaqI TCGA 3 cut(s) 63, 119, 373
TasI AATT 4 cut(s) 113, 260, 496, 664
TatI WGTACW 1 cut(s) 492
TfiI GAWTC 1 cut(s) 461
Tru1I TTAA 3 cut(s) 99, 363, 441
Tru9I TTAA 3 cut(s) 99, 363, 441
TscAI CASTG 2 cut(s) 229, 306
TseFI GTSAC 2 cut(s) 220, 671
TseI GCWGC 1 cut(s) 658
Tsp45I GTSAC 2 cut(s) 220, 671
TspDTI ATGAA 2 cut(s) 126, 450
TspRI CASTG 2 cut(s) 229, 306
Vha464I CTTAAG 1 cut(s) 362
XapI RAATTY 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.