FvH4_7g01340

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005826
Physical Location & Seq
Forward (+)
1761359 .. 1767246
5888 bp
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UTR
Exon/CDS
Intron
FvH4_7g01340.t1

Sequence Viewer

Length: 549 bp
ATGAGCGATCAGAATAGTGGTTCAGTGTGGAAAGGTGGAGTTCCAAGGGGTGGCTCCTCTGGGAAACAGGAAACAGTTGGATCTTTGTCTGTCACTGTTATTGATGCCTCATCTACGAACGAATACTCACAATTTCCCAAAAAAGCACCATCAGGAGATGGTGGAAAGTCAGCGAAGCCTACAGCTCGTGCCAAAGTTCCTTTTGAGAAGGGGTATAGCCAAATGGATTGGCTCAAGCTTACTCGTACGCATCCTGACCTAGCAGGTTTGCAGGGACAGTCAAATAAGAGGCTTATTACCATGAATGAAGTTAAACAACACCAAAAAGGGGATTCCATGTGGACTGTTCTAAAAGGCCGTGTGTACAATTTATCTCCATACATGAGATTTCACCCTGGAGGTGTTGATATTCTAAAGAAGGCTGTGGGAAAAGACTGCACATCTTTATTCAATAAATACCATGCTTGGGTGAATGCTGATTTTTTACTTGAGAAGTGTCTGGTTGGTACTTTAGATGATGGACAAGGGCAGCAAGATTCCAGTCACTAA

Protein Analysis

183

Amino Acids

19.83

Weight (kDa)

9.39

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 100 - 172 2.1e-23 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 50
AclWI GGATC 1 cut(s) 88
AfaI GTAC 3 cut(s) 247, 365, 508
AfiI CCNNNNNNNGG 3 cut(s) 50, 328, 466
AgsI TTSAA 1 cut(s) 451
AjnI CCWGG 1 cut(s) 394
AluBI AGCT 2 cut(s) 185, 238
AluI AGCT 2 cut(s) 185, 238
AlwI GGATC 1 cut(s) 88
AoxI GGCC 1 cut(s) 355
ApeKI GCWGC 1 cut(s) 529
AsuHPI GGTGA 2 cut(s) 383, 481
BauI CACGAG 1 cut(s) 186
BbvI GCAGC 1 cut(s) 541
BccI CCATC 3 cut(s) 152, 157, 512
BceAI ACGGC 1 cut(s) 342
BciT130I CCWGG 1 cut(s) 396
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 254
BisI GCNGC 1 cut(s) 530
BlsI GCNGC 1 cut(s) 531
Bme1390I CCNGG 1 cut(s) 396
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 396
BmsI GCATC 2 cut(s) 94, 259
BpmI CTGGAG 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 218, 509
BsaJI CCNNGG 2 cut(s) 44, 394
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 328, 466
Bse1I ACTGG 1 cut(s) 540
BseBI CCWGG 1 cut(s) 396
BseDI CCNNGG 2 cut(s) 44, 394
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 3 cut(s) 50, 328, 466
BseNI ACTGG 1 cut(s) 540
BseRI GAGGAG 1 cut(s) 46
BseXI GCAGC 1 cut(s) 541
BsgI GTGCAG 1 cut(s) 421
BshFI GGCC 1 cut(s) 357
BsiWI CGTACG 1 cut(s) 245
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 3 cut(s) 50, 328, 466
BsmFI GGGAC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 1 cut(s) 357
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 2 cut(s) 7, 80
BspANI GGCC 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 1 cut(s) 88
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 1 cut(s) 540
BssECI CCNNGG 2 cut(s) 44, 394
BssMI GATC 2 cut(s) 7, 80
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 44
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 4 cut(s) 76, 97, 279, 346
BstAUI TGTACA 1 cut(s) 363
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 2 cut(s) 10, 83
BstMBI GATC 2 cut(s) 7, 80
BstNI CCWGG 1 cut(s) 396
BstSCI CCNGG 1 cut(s) 394
BstSFI CTRYAG 1 cut(s) 180
BstV1I GCAGC 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
BsuRI GGCC 1 cut(s) 357
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 2 cut(s) 30, 93
BveI ACCTGC 1 cut(s) 254
Csp6I GTAC 3 cut(s) 246, 364, 507
CviAII CATG 4 cut(s) 301, 337, 382, 461
CviJI RGCY 9 cut(s) 54, 178, 185, 219, 232, 238, 292, 357, 422
CviKI_1 RGCY 9 cut(s) 54, 178, 185, 219, 232, 238, 292, 357, 422
CviQI GTAC 3 cut(s) 246, 364, 507
DpnI GATC 2 cut(s) 9, 82
DpnII GATC 2 cut(s) 7, 80
Eco130I CCWWGG 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 4 cut(s) 304, 340, 385, 464
FaiI YATR 6 cut(s) 216, 302, 338, 379, 383, 462
FaqI GGGAC 1 cut(s) 288
FatI CATG 4 cut(s) 300, 336, 381, 460
Fnu4HI GCNGC 1 cut(s) 530
FokI GGATG 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 530
FspBI CTAG 1 cut(s) 260
GluI GCNGC 1 cut(s) 530
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 1 cut(s) 357
Hin1II CATG 4 cut(s) 304, 340, 385, 464
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 2 cut(s) 332, 536
HphI GGTGA 2 cut(s) 383, 481
Hpy166II GTNNAC 2 cut(s) 342, 364
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 2 cut(s) 153, 254
Hpy8I GTNNAC 2 cut(s) 342, 364
HpyAV CCTTC 2 cut(s) 202, 412
HpyCH4III ACNGT 4 cut(s) 76, 97, 279, 346
HpyCH4V TGCA 2 cut(s) 271, 438
Hsp92II CATG 4 cut(s) 304, 340, 385, 464
Kzo9I GATC 2 cut(s) 7, 80
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 9 cut(s) 45, 53, 138, 249, 257, 267, 381, 408, 485
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 2 cut(s) 94, 259
MaeI CTAG 1 cut(s) 260
MaeIII GTNAC 2 cut(s) 91, 542
MalI GATC 2 cut(s) 9, 82
MboI GATC 2 cut(s) 7, 80
MflI RGATCY 1 cut(s) 80
MluCI AATT 2 cut(s) 131, 367
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 4 cut(s) 67, 118, 282, 392
MseI TTAA 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 396
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 1 cut(s) 396
NdeII GATC 2 cut(s) 7, 80
NlaIII CATG 4 cut(s) 304, 340, 385, 464
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 2 cut(s) 91, 542
PctI GAATGC 1 cut(s) 478
PfeI GAWTC 2 cut(s) 332, 536
Pfl23II CGTACG 1 cut(s) 245
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 1 cut(s) 531
Psp6I CCWGG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 394
PspLI CGTACG 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 55
PsuI RGATCY 1 cut(s) 80
RsaI GTAC 3 cut(s) 247, 365, 508
RsaNI GTAC 3 cut(s) 246, 364, 507
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 1 cut(s) 530
Sau3AI GATC 2 cut(s) 7, 80
ScrFI CCNGG 1 cut(s) 396
SetI ASST 6 cut(s) 37, 187, 240, 261, 268, 403
SfaNI GCATC 2 cut(s) 94, 259
SfcI CTRYAG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 233, 488
SmoI CTYRAG 2 cut(s) 233, 488
Sse9I AATT 2 cut(s) 131, 367
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 1 cut(s) 394
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 4 cut(s) 76, 97, 279, 346
TasI AATT 2 cut(s) 131, 367
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 2 cut(s) 332, 536
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 2 cut(s) 30, 100
TseFI GTSAC 2 cut(s) 91, 542
TseI GCWGC 1 cut(s) 529
Tsp45I GTSAC 2 cut(s) 91, 542
TspDTI ATGAA 2 cut(s) 317, 321
TspRI CASTG 2 cut(s) 30, 100
Van91I CCANNNNNTGG 1 cut(s) 50
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.