MD07G1017800.v1.1

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005826
Physical Location & Seq
Forward (+)
1536602 .. 1540942
4341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1017800.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGACAATGATAACGATTTCACTTTTTGCCAGGTTGGCCCACCAGGGAACCAGGAAGATCATGAGATAAAGAAGCTCACTTCAGATATAGAAAGCATTACCATAAAGGGTGGATTGTCAAATGGCACTAGTAGTAATGAGAATAGAGGTGTTCTTTGGCAAGGTGGATTACCAGGAGGAGCCACTTCCAAAAAAATGGAAATAGTTGGTTCTTTGTCTGTCAGCGCCATTGATGAATCATCCATGAAAAAGAAATCTGAAGTTCCAAAGCAACTAAAATCAGGTGATGCTGGAAACTCAGTCAAGCCTGCAACTCGTGCTAAAGTTCCTTTTGAGAAGGGATATAGCCAAATGGATTGGCTCAAACTTACTCGAACACATCCTGACCTTGCAGGCTTAAAGGGACAGTCAAATAAGAGGCTTATTTCACTGAATGAAGTTAAACAGCACCAAAAAGGAGATTCAATATGGACTGTTCTAAAAGGTCGCGTGTACAATTTGTCTCCATACATGAGATTTCACCCTGGAGGTGTTGATATTCTAAAGAAGGCTGTGGGAAAAGACTGCACTTCGTTATTCAATATATACCATGCTTGGGTGCATGCTGAATTTTTACTCGAGAAATGTCTTGTTGGTACTTTTAGACGATGGCGAAGGACAACACTCAAATCATTAGAGCAAGTCCACCCCTAA

Protein Analysis

231

Amino Acids

25.54

Weight (kDa)

9.5

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 144 - 214 5.8e-22 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 489
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 2 cut(s) 66, 279
AfaI GTAC 2 cut(s) 494, 637
AfiI CCNNNNNNNGG 1 cut(s) 595
AgsI TTSAA 2 cut(s) 465, 580
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 5 cut(s) 30, 43, 51, 172, 523
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw26I GTCTC 1 cut(s) 507
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 608
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 296, 512
AvaI CYCGRG 1 cut(s) 617
BauI CACGAG 1 cut(s) 315
BccI CCATC 1 cut(s) 642
BciT130I CCWGG 5 cut(s) 32, 45, 53, 174, 525
BcoDI GTCTC 1 cut(s) 507
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 1 cut(s) 129
BfoI RGCGCY 1 cut(s) 228
BglI GCCNNNNNGGC 1 cut(s) 36
Bme1390I CCNGG 5 cut(s) 32, 45, 53, 174, 525
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 1 cut(s) 38
BmiI GGNNCC 2 cut(s) 50, 181
BmrFI CCNGG 5 cut(s) 32, 45, 53, 174, 525
BmsI GCATC 1 cut(s) 277
BpmI CTGGAG 1 cut(s) 546
BsaJI CCNNGG 2 cut(s) 44, 523
Bsc4I CCNNNNNNNGG 1 cut(s) 595
BseBI CCWGG 5 cut(s) 32, 45, 53, 174, 525
BseDI CCNNGG 2 cut(s) 44, 523
BseGI GGATG 2 cut(s) 239, 379
BseLI CCNNNNNNNGG 1 cut(s) 595
BseMII CTCAG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 192
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 617
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 595
BsmAI GTCTC 1 cut(s) 507
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 1 cut(s) 39
BsoBI CYCGRG 1 cut(s) 617
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 1 cut(s) 58
BspANI GGCC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 311
BspFNI CGCG 1 cut(s) 489
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 50, 181
BsrGI TGTACA 1 cut(s) 492
BssECI CCNNGG 2 cut(s) 44, 523
BssMI GATC 1 cut(s) 58
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 5 cut(s) 32, 45, 53, 174, 525
Bst4CI ACNGT 2 cut(s) 408, 475
BstAPI GCANNNNNTGC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 3 cut(s) 309, 394, 603
BstDEI CTNAG 1 cut(s) 298
BstF5I GGATG 2 cut(s) 239, 379
BstFNI CGCG 1 cut(s) 489
BstH2I RGCGCY 1 cut(s) 228
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 1 cut(s) 61
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 1 cut(s) 58
BstMWI GCNNNNNNNGC 2 cut(s) 36, 317
BstNI CCWGG 5 cut(s) 32, 45, 53, 174, 525
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 5 cut(s) 30, 43, 51, 172, 523
BstUI CGCG 1 cut(s) 489
BstXI CCANNNNNNTGG 1 cut(s) 196
BsuRI GGCC 1 cut(s) 39
BtsCI GGATG 2 cut(s) 239, 379
BtsIMutI CAGTG 1 cut(s) 428
Cac8I GCNNGC 3 cut(s) 309, 394, 603
CciI TCATGA 1 cut(s) 61
CfoI GCGC 1 cut(s) 227
Cfr13I GGNCC 1 cut(s) 38
Csp6I GTAC 2 cut(s) 493, 636
CviAII CATG 5 cut(s) 62, 244, 511, 590, 602
CviJI RGCY 9 cut(s) 39, 76, 182, 307, 348, 361, 396, 421, 551
CviKI_1 RGCY 9 cut(s) 39, 76, 182, 307, 348, 361, 396, 421, 551
CviQI GTAC 2 cut(s) 493, 636
DdeI CTNAG 1 cut(s) 298
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
Eco57I CTGAAG 2 cut(s) 66, 279
Eco88I CYCGRG 1 cut(s) 617
EcoRII CCWGG 5 cut(s) 30, 43, 51, 172, 523
FaeI CATG 5 cut(s) 65, 247, 514, 593, 605
FaqI GGGAC 1 cut(s) 417
FatI CATG 5 cut(s) 61, 243, 510, 589, 601
FokI GGATG 2 cut(s) 226, 366
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 226
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 228
HaeIII GGCC 1 cut(s) 39
HhaI GCGC 1 cut(s) 227
Hin1II CATG 5 cut(s) 65, 247, 514, 593, 605
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HinfI GANTC 2 cut(s) 236, 461
HphI GGTGA 2 cut(s) 296, 512
Hpy166II GTNNAC 2 cut(s) 493, 685
Hpy188I TCNGA 2 cut(s) 85, 259
Hpy188III TCNNGA 3 cut(s) 62, 383, 619
Hpy8I GTNNAC 2 cut(s) 493, 685
HpyAV CCTTC 3 cut(s) 331, 541, 648
HpyCH4III ACNGT 2 cut(s) 408, 475
HpyCH4V TGCA 4 cut(s) 311, 392, 567, 601
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 317
HpyF3I CTNAG 1 cut(s) 298
Hsp92II CATG 5 cut(s) 65, 247, 514, 593, 605
HspAI GCGC 1 cut(s) 225
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 1 cut(s) 179
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 129
MalI GATC 1 cut(s) 60
MboI GATC 1 cut(s) 58
MboII GAAGA 1 cut(s) 68
MluCI AATT 2 cut(s) 496, 608
MnlI CCTC 4 cut(s) 140, 170, 411, 521
MseI TTAA 2 cut(s) 398, 441
MspR9I CCNGG 5 cut(s) 32, 45, 53, 174, 525
MvaI CCWGG 5 cut(s) 32, 45, 53, 174, 525
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 2 cut(s) 36, 317
NdeII GATC 1 cut(s) 58
NlaIII CATG 5 cut(s) 65, 247, 514, 593, 605
NlaIV GGNNCC 2 cut(s) 50, 181
NspI RCATGY 1 cut(s) 605
PaeI GCATGC 1 cut(s) 605
PaeR7I CTCGAG 1 cut(s) 617
PagI TCATGA 1 cut(s) 61
PfeI GAWTC 2 cut(s) 236, 461
Psp6I CCWGG 5 cut(s) 30, 43, 51, 172, 523
PspGI CCWGG 5 cut(s) 30, 43, 51, 172, 523
PspN4I GGNNCC 2 cut(s) 50, 181
PspPI GGNCC 1 cut(s) 38
RsaI GTAC 2 cut(s) 494, 637
RsaNI GTAC 2 cut(s) 493, 636
SaqAI TTAA 2 cut(s) 398, 441
Sau3AI GATC 1 cut(s) 58
Sau96I GGNCC 1 cut(s) 38
ScrFI CCNGG 5 cut(s) 32, 45, 53, 174, 525
SetI ASST 8 cut(s) 36, 78, 151, 166, 286, 390, 487, 532
SfaNI GCATC 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 617
SlaI CTCGAG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SpeI ACTAGT 1 cut(s) 128
SphI GCATGC 1 cut(s) 605
Sse9I AATT 2 cut(s) 496, 608
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 5 cut(s) 30, 43, 51, 172, 523
TaaI ACNGT 2 cut(s) 408, 475
TaqI TCGA 2 cut(s) 373, 618
TasI AATT 2 cut(s) 496, 608
TatI WGTACW 1 cut(s) 492
TfiI GAWTC 2 cut(s) 236, 461
Tru1I TTAA 2 cut(s) 398, 441
Tru9I TTAA 2 cut(s) 398, 441
TscAI CASTG 1 cut(s) 435
TspDTI ATGAA 3 cut(s) 249, 260, 450
TspRI CASTG 1 cut(s) 435
XapI RAATTY 1 cut(s) 608
XceI RCATGY 1 cut(s) 605
XhoI CTCGAG 1 cut(s) 617
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.