Rw1G001750

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005826
Physical Location & Seq
Forward (+)
3015739 .. 3020484
4746 bp
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UTR
Exon/CDS
Intron
Rw1G001750.1

Sequence Viewer

Length: 444 bp
ATGTGGAAAGGTGGAGTGCCAAGGGGTGCCACCTCTGAAAAACAGAAAACAGTTGGATCTTTGTCTGTCACTGTTATTGATGCGTCATCCACGAATGAATACTCACAATTTCCAAAAAAAGTAGCATCAGGAGATGGTGGAAAGTCAGTGAAGCCTGCACCTCGTGCCAAAGTTCCTTTTGAGAAGGGGTATAGCCAAATGGATTGGCTTAAGCTTACTCGAACACATCCTGACCTTGCAGGTTTGAAGGGACAGTCAAATAAGAGGCTTATTTCCATGAATGAAGTTAAACAGCACCAAAAAGGGGATTCTATGTGGACTGTTCTAAAAGGCCGTGTGTACAACTTATCTCCATACATGAGATTTCACCCTGGAGGTGTTGATATTCTAAAGAAGGCTGTGGGGAAAGACTGCACTTCTTTATTCAGTATCCTTTATATATAA

Protein Analysis

147

Amino Acids

16.19

Weight (kDa)

9.97

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 93 - 143 7.7e-17 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 230
AccB1I GGYRCC 1 cut(s) 26
AclWI GGATC 1 cut(s) 64
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 304
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 1 cut(s) 214
AluI AGCT 1 cut(s) 214
AlwI GGATC 1 cut(s) 64
AoxI GGCC 1 cut(s) 331
AsuHPI GGTGA 1 cut(s) 359
BanI GGYRCC 1 cut(s) 26
BauI CACGAG 1 cut(s) 162
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 318
BciT130I CCWGG 1 cut(s) 372
BciVI GTATCC 1 cut(s) 440
BfrI CTTAAG 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 230
BfuI GTATCC 1 cut(s) 440
Bme1390I CCNGG 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 2 cut(s) 70, 134
BpmI CTGGAG 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 20, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 304
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 20, 370
BseGI GGATG 2 cut(s) 86, 226
BseLI CCNNNNNNNGG 1 cut(s) 304
BsgI GTGCAG 2 cut(s) 141, 397
BshFI GGCC 1 cut(s) 333
BshNI GGYRCC 1 cut(s) 26
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 1 cut(s) 333
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 1 cut(s) 56
BspANI GGCC 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 28
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 209
BsrGI TGTACA 1 cut(s) 339
BssECI CCNNGG 2 cut(s) 20, 370
BssMI GATC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 20
Bst2BI CACGAG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 4 cut(s) 52, 73, 255, 322
BstAFI CTTAAG 1 cut(s) 209
BstAPI GCANNNNNTGC 1 cut(s) 164
BstAUI TGTACA 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 156
BstF5I GGATG 2 cut(s) 86, 226
BstKTI GATC 1 cut(s) 59
BstMBI GATC 1 cut(s) 56
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 370
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 1 cut(s) 333
BtsCI GGATG 2 cut(s) 86, 226
BtsIMutI CAGTG 2 cut(s) 69, 153
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 156
CseI GACGC 1 cut(s) 72
Csp6I GTAC 1 cut(s) 340
CviAII CATG 2 cut(s) 277, 358
CviJI RGCY 7 cut(s) 154, 195, 208, 214, 268, 333, 398
CviKI_1 RGCY 7 cut(s) 154, 195, 208, 214, 268, 333, 398
CviQI GTAC 1 cut(s) 340
DpnI GATC 1 cut(s) 58
DpnII GATC 1 cut(s) 56
DraIII CACNNNGTG 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 2 cut(s) 280, 361
FaiI YATR 8 cut(s) 192, 278, 314, 355, 359, 438, 440, 442
FaqI GGGAC 1 cut(s) 264
FatI CATG 2 cut(s) 276, 357
FokI GGATG 2 cut(s) 73, 213
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 1 cut(s) 333
HgaI GACGC 1 cut(s) 72
Hin1II CATG 2 cut(s) 280, 361
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 1 cut(s) 308
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 2 cut(s) 318, 340
Hpy188I TCNGA 1 cut(s) 37
Hpy188III TCNNGA 2 cut(s) 129, 230
Hpy8I GTNNAC 2 cut(s) 318, 340
HpyAV CCTTC 3 cut(s) 178, 241, 388
HpyCH4III ACNGT 4 cut(s) 52, 73, 255, 322
HpyCH4V TGCA 3 cut(s) 158, 239, 414
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
Hsp92II CATG 2 cut(s) 280, 361
Kzo9I GATC 1 cut(s) 56
LpnPI CCDG 6 cut(s) 114, 168, 225, 243, 357, 384
LweI GCATC 2 cut(s) 70, 134
MaeIII GTNAC 1 cut(s) 67
MalI GATC 1 cut(s) 58
MboI GATC 1 cut(s) 56
MflI RGATCY 1 cut(s) 56
MluCI AATT 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 4 cut(s) 43, 171, 258, 368
MseI TTAA 2 cut(s) 210, 288
MspCI CTTAAG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 1 cut(s) 56
NlaIII CATG 2 cut(s) 280, 361
NlaIV GGNNCC 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 67
PfeI GAWTC 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 28
PsuI RGATCY 1 cut(s) 56
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SaqAI TTAA 2 cut(s) 210, 288
Sau3AI GATC 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 372
SetI ASST 7 cut(s) 13, 35, 163, 216, 237, 244, 379
SfaNI GCATC 2 cut(s) 70, 134
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 1 cut(s) 107
StyD4I CCNGG 1 cut(s) 370
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 4 cut(s) 52, 73, 255, 322
TaqI TCGA 1 cut(s) 220
TasI AATT 1 cut(s) 107
TatI WGTACW 1 cut(s) 339
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 210, 288
Tru9I TTAA 2 cut(s) 210, 288
TscAI CASTG 2 cut(s) 76, 153
TseFI GTSAC 1 cut(s) 67
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 3 cut(s) 111, 293, 297
TspRI CASTG 2 cut(s) 76, 153
Vha464I CTTAAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.