Rh1DG022400

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005826
Physical Location & Seq
Forward (+)
3457068 .. 3461831
4764 bp
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UTR
Exon/CDS
Intron
Rh1DG022400.1

Sequence Viewer

Length: 558 bp
ATGGACACTGATAACGATTTCACGTTTTGCCAGGTTGGCTCACCTGAAAACCAGGATGCTCTCGAAACAAAGAAGCTTGCTTCAGATATAGATGCTATTAACATAAACGATGGATTGTCGAATGGCACTATTAGCAATCAGAATAGTGGTTCAATGTGGAAAGGTGGAGTGCCAAGGGGTGCCCCCTCTGAAAAACAGAAAACAGTTGGATCTTTGTCTGTCACTGTTATTGATGCGTCATCCACGAACGAATACTCCCAATTTCCAAAAAAAGTACCATCAGGAGATGGTGGAAAGTCAGTGAAGCCTGCACCTCGTGCCAAAGTTCCTTTTGAGAAGGGGTATAGCCAAATGGATTGGCTTAAGCTTACTCGAACACATCCTGACCTTGCAGGTTTGAAGGGACAGTCAAATAAGAGGCTTATTTCCATGAATGAAGTTAAACAGCACCAAAAAGGGGATTCTATGTGGACTGTTCTAAAAGGCCGTGTGTACAACTTATCTCCATACATGAGATTTCACCCTGGAGGTATGGTCATGGTTTCCACATTTCGATAG

Protein Analysis

185

Amino Acids

20.24

Weight (kDa)

9.41

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 144 - 180 1.3e-10 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 179
AclWI GGATC 1 cut(s) 217
AcuI CTGAAG 1 cut(s) 66
AdeI CACNNNGTG 1 cut(s) 317
AfaI GTAC 2 cut(s) 276, 494
AfiI CCNNNNNNNGG 1 cut(s) 457
AflII CTTAAG 1 cut(s) 362
AgsI TTSAA 2 cut(s) 153, 400
AjnI CCWGG 3 cut(s) 30, 51, 523
AloI GAACNNNNNNTCC 2 cut(s) 239, 271
AluBI AGCT 2 cut(s) 76, 367
AluI AGCT 2 cut(s) 76, 367
AlwI GGATC 1 cut(s) 217
AoxI GGCC 1 cut(s) 484
AsuHPI GGTGA 2 cut(s) 33, 512
BaeGI GKGCMC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 179
BauI CACGAG 1 cut(s) 315
BccI CCATC 3 cut(s) 104, 281, 286
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 3 cut(s) 32, 53, 525
BfrI CTTAAG 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 383
BglI GCCNNNNNGGC 1 cut(s) 36
Bme1390I CCNGG 3 cut(s) 32, 53, 525
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 3 cut(s) 32, 53, 525
BmsI GCATC 3 cut(s) 46, 82, 223
BpmI CTGGAG 1 cut(s) 546
BsaJI CCNNGG 2 cut(s) 173, 523
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 519, 549
Bsc4I CCNNNNNNNGG 1 cut(s) 457
BseBI CCWGG 3 cut(s) 32, 53, 525
BseDI CCNNGG 2 cut(s) 173, 523
BseGI GGATG 3 cut(s) 61, 239, 379
BseLI CCNNNNNNNGG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 294
BshFI GGCC 1 cut(s) 486
BshNI GGYRCC 1 cut(s) 179
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 1 cut(s) 486
Bsp1286I GDGCHC 1 cut(s) 184
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 1 cut(s) 209
BspANI GGCC 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 383
BspPI GGATC 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 179
BspTI CTTAAG 1 cut(s) 362
BsrGI TGTACA 1 cut(s) 492
BssECI CCNNGG 2 cut(s) 173, 523
BssMI GATC 1 cut(s) 209
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 173
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 3 cut(s) 32, 53, 525
Bst4CI ACNGT 4 cut(s) 205, 226, 408, 475
BstAFI CTTAAG 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 78, 309
BstF5I GGATG 3 cut(s) 61, 239, 379
BstKTI GATC 1 cut(s) 212
BstMBI GATC 1 cut(s) 209
BstMWI GCNNNNNNNGC 3 cut(s) 36, 132, 317
BstNI CCWGG 3 cut(s) 32, 53, 525
BstSCI CCNGG 3 cut(s) 30, 51, 523
BstSLI GKGCMC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
BsuRI GGCC 1 cut(s) 486
BtsCI GGATG 3 cut(s) 61, 239, 379
BtsIMutI CAGTG 3 cut(s) 6, 222, 306
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 2 cut(s) 78, 309
CseI GACGC 1 cut(s) 225
Csp6I GTAC 2 cut(s) 275, 493
CviAII CATG 3 cut(s) 430, 511, 538
CviJI RGCY 8 cut(s) 39, 76, 307, 348, 361, 367, 421, 486
CviKI_1 RGCY 8 cut(s) 39, 76, 307, 348, 361, 367, 421, 486
CviQI GTAC 2 cut(s) 275, 493
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
DraIII CACNNNGTG 1 cut(s) 317
Eco130I CCWWGG 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 30, 51, 523
EcoT14I CCWWGG 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 3 cut(s) 433, 514, 541
FaiI YATR 9 cut(s) 89, 104, 345, 431, 467, 508, 512, 533, 539
FaqI GGGAC 1 cut(s) 417
FatI CATG 3 cut(s) 429, 510, 537
FokI GGATG 3 cut(s) 68, 226, 366
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 1 cut(s) 486
HgaI GACGC 1 cut(s) 225
Hin1II CATG 3 cut(s) 433, 514, 541
HindIII AAGCTT 2 cut(s) 74, 365
HinfI GANTC 1 cut(s) 461
HphI GGTGA 2 cut(s) 33, 512
Hpy166II GTNNAC 2 cut(s) 471, 493
Hpy188I TCNGA 3 cut(s) 85, 141, 190
Hpy188III TCNNGA 3 cut(s) 62, 282, 383
Hpy8I GTNNAC 2 cut(s) 471, 493
HpyAV CCTTC 2 cut(s) 331, 394
HpyCH4III ACNGT 4 cut(s) 205, 226, 408, 475
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 2 cut(s) 311, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 132, 317
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 3 cut(s) 433, 514, 541
Kzo9I GATC 1 cut(s) 209
LweI GCATC 3 cut(s) 46, 82, 223
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 220
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 4 cut(s) 196, 324, 411, 521
MseI TTAA 3 cut(s) 99, 363, 441
MspCI CTTAAG 1 cut(s) 362
MspR9I CCNGG 3 cut(s) 32, 53, 525
MvaI CCWGG 3 cut(s) 32, 53, 525
MwoI GCNNNNNNNGC 3 cut(s) 36, 132, 317
NdeII GATC 1 cut(s) 209
NlaIII CATG 3 cut(s) 433, 514, 541
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 461
Psp6I CCWGG 3 cut(s) 30, 51, 523
PspGI CCWGG 3 cut(s) 30, 51, 523
PspN4I GGNNCC 1 cut(s) 181
PsuI RGATCY 1 cut(s) 209
RsaI GTAC 2 cut(s) 276, 494
RsaNI GTAC 2 cut(s) 275, 493
SaqAI TTAA 3 cut(s) 99, 363, 441
Sau3AI GATC 1 cut(s) 209
ScrFI CCNGG 3 cut(s) 32, 53, 525
SduI GDGCHC 1 cut(s) 184
SfaNI GCATC 3 cut(s) 46, 82, 223
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 1 cut(s) 260
StyD4I CCNGG 3 cut(s) 30, 51, 523
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 4 cut(s) 205, 226, 408, 475
TaiI ACGT 1 cut(s) 26
TaqI TCGA 4 cut(s) 63, 119, 373, 553
TasI AATT 1 cut(s) 260
TatI WGTACW 1 cut(s) 492
TfiI GAWTC 1 cut(s) 461
Tru1I TTAA 3 cut(s) 99, 363, 441
Tru9I TTAA 3 cut(s) 99, 363, 441
TscAI CASTG 3 cut(s) 13, 229, 306
TseFI GTSAC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 446, 450
TspRI CASTG 3 cut(s) 13, 229, 306
Vha464I CTTAAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.