Rh1AG025200

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005826
Physical Location & Seq
Forward (+)
4614771 .. 4621692
6922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG025200.1

Sequence Viewer

Length: 825 bp
ATGCCAGTTTCCGGCCAGTATACTTGGTTTACCATTAATTGTGATACTGGACATAAGTTCTCAGAATTAGCGCGATCAAGTCAAAGAGTCTCATCCTGTTTCGGAAAGATTGCTCGAAATAATCTGGGAACTTCATTCAGCTTCGGAATGGACACTGATAACGATTTCACATTTTGCCAGGTTGGCTCACCTGAAAACCAGGATGCTCTCGAAACAAAGAAGCTTGCTTCAGATATAGATGCTATTAACATAAACGATGGATTGTCGAATGGCACTATTAGCAATCAGAATAGTGGTTCAATGTGGAAAGGTGGAGTGCCAAGGGGTGCCACCTCTGAAAAACAGAAAACAGTTGGATCTTTGTCTGTCACTGTTATTGATGCGTCATCCACGAACGAATACTCACAATTTCCAAAAAAAGTACCATCAGGAGATGGTGGAAAGTCAGTGAAGCCTGCACCTCGTGCCAAAGTTCCTTTTGAGAAGGGATATAGCCAAATGGATTGGCTTAAGCTTACTCGAACACATCCTGACCTTGCAGGTTTGAAGGGACAGTCAAATAAGAGGCTTATTTCCATGAATGAAGTTAAACAGCACCAAAAAGGGGATTCTATGTGGACTGTTCTAAAAGGCCGTGTGTACAATTTATCTCCATACATGAGATTTCACCCTGGAGGTGTTGATATTCTAAAGAAGGCTGTGGGGAAAGACTGCACTTCTTTATTCAATAAATACCATGCTTGGGTCAATGCTGATTTTTTACTTGAGAAGTGTCTCGTTGGTACTTTAGATGATGGACAAGGGCAGCAAAATTCCAGTCACTAA

Protein Analysis

274

Amino Acids

29.99

Weight (kDa)

9.09

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 193 - 264 6.8e-23 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 530
AccB1I GGYRCC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 20
AccII CGCG 1 cut(s) 73
AclWI GGATC 1 cut(s) 364
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 1 cut(s) 811
AcuI CTGAAG 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 464
AfaI GTAC 3 cut(s) 423, 641, 784
AfiI CCNNNNNNNGG 3 cut(s) 11, 604, 742
AflII CTTAAG 1 cut(s) 509
AgsI TTSAA 3 cut(s) 300, 547, 727
AjnI CCWGG 3 cut(s) 177, 198, 670
AluBI AGCT 3 cut(s) 141, 223, 514
AluI AGCT 3 cut(s) 141, 223, 514
Alw26I GTCTC 2 cut(s) 94, 779
AlwI GGATC 1 cut(s) 364
AoxI GGCC 2 cut(s) 13, 631
ApeKI GCWGC 1 cut(s) 805
ApoI RAATTY 1 cut(s) 811
ArsI GACNNNNNNTTYG 1 cut(s) 802
AseI ATTAAT 1 cut(s) 36
AspLEI GCGC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 180, 659
BanI GGYRCC 1 cut(s) 326
BauI CACGAG 1 cut(s) 462
BbvI GCAGC 1 cut(s) 817
BccI CCATC 4 cut(s) 251, 428, 433, 788
BceAI ACGGC 1 cut(s) 618
BciT130I CCWGG 3 cut(s) 179, 200, 672
BcoDI GTCTC 2 cut(s) 94, 779
BfrI CTTAAG 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 530
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 1 cut(s) 806
BlsI GCNGC 1 cut(s) 807
Bme1390I CCNGG 3 cut(s) 179, 200, 672
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 3 cut(s) 179, 200, 672
BmsI GCATC 3 cut(s) 193, 229, 370
BpmI CTGGAG 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 785
BsaJI CCNNGG 2 cut(s) 320, 670
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 604, 742
Bse1I ACTGG 4 cut(s) 5, 16, 52, 816
BseBI CCWGG 3 cut(s) 179, 200, 672
BseDI CCNNGG 2 cut(s) 320, 670
BseGI GGATG 4 cut(s) 92, 208, 386, 526
BseLI CCNNNNNNNGG 3 cut(s) 11, 604, 742
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 4 cut(s) 5, 16, 52, 816
BseXI GCAGC 1 cut(s) 817
BsgI GTGCAG 2 cut(s) 441, 697
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 2 cut(s) 15, 633
BshNI GGYRCC 1 cut(s) 326
BsiSI CCGG 1 cut(s) 12
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 3 cut(s) 11, 604, 742
BsmAI GTCTC 2 cut(s) 94, 779
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 2 cut(s) 15, 633
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 2 cut(s) 74, 356
BspANI GGCC 2 cut(s) 15, 633
BspCNI CTCAG 1 cut(s) 74
BspFNI CGCG 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 328
BspMI ACCTGC 1 cut(s) 530
BspPI GGATC 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 326
BspTI CTTAAG 1 cut(s) 509
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 4 cut(s) 5, 16, 52, 816
BssECI CCNNGG 2 cut(s) 320, 670
BssMI GATC 2 cut(s) 74, 356
BssNAI GTATAC 1 cut(s) 21
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 320
Bst1107I GTATAC 1 cut(s) 21
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 3 cut(s) 179, 200, 672
Bst4CI ACNGT 4 cut(s) 352, 373, 555, 622
BstAFI CTTAAG 1 cut(s) 509
BstAPI GCANNNNNTGC 1 cut(s) 464
BstAUI TGTACA 1 cut(s) 639
BstC8I GCNNGC 2 cut(s) 225, 456
BstDEI CTNAG 1 cut(s) 61
BstF5I GGATG 4 cut(s) 92, 208, 386, 526
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 2 cut(s) 77, 359
BstMAI GTCTC 2 cut(s) 94, 779
BstMBI GATC 2 cut(s) 74, 356
BstMWI GCNNNNNNNGC 3 cut(s) 183, 279, 464
BstNI CCWGG 3 cut(s) 179, 200, 672
BstSCI CCNGG 3 cut(s) 177, 198, 670
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 1 cut(s) 817
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
BstZ17I GTATAC 1 cut(s) 21
BsuRI GGCC 2 cut(s) 15, 633
BtsCI GGATG 4 cut(s) 92, 208, 386, 526
BtsIMutI CAGTG 3 cut(s) 153, 369, 453
BveI ACCTGC 1 cut(s) 530
Cac8I GCNNGC 2 cut(s) 225, 456
CfoI GCGC 1 cut(s) 73
CseI GACGC 1 cut(s) 372
Csp6I GTAC 3 cut(s) 422, 640, 783
CviAII CATG 3 cut(s) 577, 658, 737
CviQI GTAC 3 cut(s) 422, 640, 783
DdeI CTNAG 1 cut(s) 61
DpnI GATC 2 cut(s) 76, 358
DpnII GATC 2 cut(s) 74, 356
DraIII CACNNNGTG 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 213
EcoRII CCWGG 3 cut(s) 177, 198, 670
EcoT14I CCWWGG 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 320
FaeI CATG 3 cut(s) 580, 661, 740
FaqI GGGAC 1 cut(s) 564
FatI CATG 3 cut(s) 576, 657, 736
FblI GTMKAC 1 cut(s) 20
Fnu4HI GCNGC 1 cut(s) 806
FokI GGATG 4 cut(s) 79, 215, 373, 513
Fsp4HI GCNGC 1 cut(s) 806
GlaI GCGC 1 cut(s) 72
GluI GCNGC 1 cut(s) 806
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 2 cut(s) 15, 633
HapII CCGG 1 cut(s) 12
HgaI GACGC 1 cut(s) 372
HhaI GCGC 1 cut(s) 73
Hin1II CATG 3 cut(s) 580, 661, 740
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HindIII AAGCTT 2 cut(s) 221, 512
HinfI GANTC 2 cut(s) 87, 608
HpaII CCGG 1 cut(s) 12
HphI GGTGA 2 cut(s) 180, 659
Hpy166II GTNNAC 4 cut(s) 21, 30, 618, 640
Hpy188I TCNGA 6 cut(s) 64, 104, 146, 232, 288, 337
Hpy188III TCNNGA 3 cut(s) 209, 429, 530
Hpy8I GTNNAC 4 cut(s) 21, 30, 618, 640
HpyAV CCTTC 3 cut(s) 478, 541, 688
HpyCH4III ACNGT 4 cut(s) 352, 373, 555, 622
HpyCH4V TGCA 3 cut(s) 458, 539, 714
HpyF10VI GCNNNNNNNGC 3 cut(s) 183, 279, 464
HpyF3I CTNAG 1 cut(s) 61
Hsp92II CATG 3 cut(s) 580, 661, 740
HspAI GCGC 1 cut(s) 71
Kzo9I GATC 2 cut(s) 74, 356
Lsp1109I GCAGC 1 cut(s) 817
LweI GCATC 3 cut(s) 193, 229, 370
MaeIII GTNAC 2 cut(s) 367, 818
MalI GATC 2 cut(s) 76, 358
MboI GATC 2 cut(s) 74, 356
MflI RGATCY 1 cut(s) 356
MluCI AATT 5 cut(s) 37, 65, 407, 643, 811
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 4 cut(s) 343, 471, 558, 668
MseI TTAA 4 cut(s) 36, 246, 510, 588
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 3 cut(s) 179, 200, 672
MvaI CCWGG 3 cut(s) 179, 200, 672
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 3 cut(s) 183, 279, 464
NdeII GATC 2 cut(s) 74, 356
NlaIII CATG 3 cut(s) 580, 661, 740
NlaIV GGNNCC 1 cut(s) 328
NmuCI GTSAC 2 cut(s) 367, 818
PfeI GAWTC 1 cut(s) 608
PkrI GCNGC 1 cut(s) 807
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PshBI ATTAAT 1 cut(s) 36
Psp6I CCWGG 3 cut(s) 177, 198, 670
PspGI CCWGG 3 cut(s) 177, 198, 670
PspN4I GGNNCC 1 cut(s) 328
PsuI RGATCY 1 cut(s) 356
RsaI GTAC 3 cut(s) 423, 641, 784
RsaNI GTAC 3 cut(s) 422, 640, 783
SaqAI TTAA 4 cut(s) 36, 246, 510, 588
SatI GCNGC 1 cut(s) 806
Sau3AI GATC 2 cut(s) 74, 356
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 3 cut(s) 179, 200, 672
SfaNI GCATC 3 cut(s) 193, 229, 370
SmlI CTYRAG 2 cut(s) 509, 764
SmoI CTYRAG 2 cut(s) 509, 764
Sse9I AATT 5 cut(s) 37, 65, 407, 643, 811
StyD4I CCNGG 3 cut(s) 177, 198, 670
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 4 cut(s) 352, 373, 555, 622
TaqI TCGA 4 cut(s) 115, 210, 266, 520
TasI AATT 5 cut(s) 37, 65, 407, 643, 811
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 4 cut(s) 36, 246, 510, 588
Tru9I TTAA 4 cut(s) 36, 246, 510, 588
TscAI CASTG 3 cut(s) 160, 376, 453
TseFI GTSAC 2 cut(s) 367, 818
TseI GCWGC 1 cut(s) 805
Tsp45I GTSAC 2 cut(s) 367, 818
TspDTI ATGAA 3 cut(s) 123, 593, 597
TspRI CASTG 3 cut(s) 160, 376, 453
Vha464I CTTAAG 1 cut(s) 509
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 1 cut(s) 811
XmiI GTMKAC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.