MD02G1307800.v1.1

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005826
Physical Location & Seq
Reverse (-)
36313388 .. 36317862
4475 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1307800.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGAGAATGATAACGATTTCACTTTTTGTCAGGTTGGTCCACCAGGCAACCAGGATGGTTATGAGACAAAGAAGCTCACTTCAGATATAGAAAACATTACCATAAAGGATGGATTTTCAAATGGAACTAGTAGTAATGAGAATAGAGGTGTTCTGTGGCAAGGTGGATTACCAGGAGGAGCCACTTCCAAAAAACAGGAAACAGTTGGTTCTTTGTCTGTCAGTGTCATTGATGCATCATCCATGAAAAAGAAATCTGAAGTTCCGAAGCAACTAAAATCAGGTGATGCTGGAAAGTCAGTGAAGCCTGCAACTCGTGCTAAAGTTCCTTTTGAGAAGGGGTATAGCCAAATGGATTGGCTCAAACTTACTCGAACACATCCTGACCTTGCAGGTTTAAAGGGACAATCAAATAAGAGGCTTATTTCACTGAATGAAGTTAAACAGCACCAAAAGGGAGATTCAATGTGGACTGTTCTAAAAGGCCGTGTGTACAATTTGTCTCCATACATGAGATTTCACCCTGGAGGTGTTGATATTCTAAAGAAGGCTGTGGGAAAAGACTGCACATCTTTGTTCAATAAATACCATGCTTGGGTGAATGCTGAATTTTTACTCGAGAAATGTCTCGTTGGTACTTTAGACGATGGTGAAAGGCAACATTCCAGTCATTCGGAATCTTCGGCCCCCTCATGA

Protein Analysis

232

Amino Acids

25.31

Weight (kDa)

9.1

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 144 - 215 2e-23 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 383
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 2 cut(s) 66, 279
AfaI GTAC 2 cut(s) 494, 637
AfiI CCNNNNNNNGG 1 cut(s) 595
AgsI TTSAA 3 cut(s) 120, 465, 580
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 4 cut(s) 43, 51, 172, 523
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw26I GTCTC 3 cut(s) 59, 507, 632
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 2 cut(s) 484, 684
ApoI RAATTY 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 38, 685
AsuHPI GGTGA 4 cut(s) 296, 512, 610, 662
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 1 cut(s) 38
BauI CACGAG 1 cut(s) 315
BccI CCATC 3 cut(s) 50, 104, 641
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 4 cut(s) 45, 53, 174, 525
BcoDI GTCTC 3 cut(s) 59, 507, 632
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 1 cut(s) 129
BfuAI ACCTGC 1 cut(s) 383
Bme1390I CCNGG 4 cut(s) 45, 53, 174, 525
Bme18I GGWCC 1 cut(s) 38
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 2 cut(s) 38, 685
BmiI GGNNCC 2 cut(s) 181, 687
BmrFI CCNGG 4 cut(s) 45, 53, 174, 525
BmsI GCATC 3 cut(s) 223, 245, 277
BpmI CTGGAG 1 cut(s) 546
BsaJI CCNNGG 1 cut(s) 523
Bsc4I CCNNNNNNNGG 1 cut(s) 595
Bse1I ACTGG 1 cut(s) 666
BseBI CCWGG 4 cut(s) 45, 53, 174, 525
BseDI CCNNGG 1 cut(s) 523
BseGI GGATG 4 cut(s) 61, 115, 239, 379
BseLI CCNNNNNNNGG 1 cut(s) 595
BseNI ACTGG 1 cut(s) 666
BseRI GAGGAG 1 cut(s) 192
BsgI GTGCAG 1 cut(s) 550
BshFI GGCC 2 cut(s) 486, 686
BsiHKCI CYCGRG 1 cut(s) 617
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 595
BsmAI GTCTC 3 cut(s) 59, 507, 632
BsmFI GGGAC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 2 cut(s) 486, 686
BsoBI CYCGRG 1 cut(s) 617
Bsp1407I TGTACA 1 cut(s) 492
BspANI GGCC 2 cut(s) 486, 686
BspHI TCATGA 1 cut(s) 692
BspLI GGNNCC 2 cut(s) 181, 687
BspMI ACCTGC 1 cut(s) 383
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 1 cut(s) 666
BssECI CCNNGG 1 cut(s) 523
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 4 cut(s) 45, 53, 174, 525
Bst4CI ACNGT 2 cut(s) 205, 475
BstAPI GCANNNNNTGC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 1 cut(s) 309
BstF5I GGATG 4 cut(s) 61, 115, 239, 379
BstMAI GTCTC 3 cut(s) 59, 507, 632
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNI CCWGG 4 cut(s) 45, 53, 174, 525
BstSCI CCNGG 4 cut(s) 43, 51, 172, 523
BsuRI GGCC 2 cut(s) 486, 686
BtsCI GGATG 4 cut(s) 61, 115, 239, 379
BtsIMutI CAGTG 3 cut(s) 229, 306, 428
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 1 cut(s) 309
CciI TCATGA 1 cut(s) 692
Cfr13I GGNCC 2 cut(s) 38, 685
Csp6I GTAC 2 cut(s) 493, 636
CviAII CATG 4 cut(s) 244, 511, 590, 693
CviJI RGCY 9 cut(s) 76, 182, 307, 348, 361, 421, 486, 551, 686
CviKI_1 RGCY 9 cut(s) 76, 182, 307, 348, 361, 421, 486, 551, 686
CviQI GTAC 2 cut(s) 493, 636
DraI TTTAAA 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 38
Eco57I CTGAAG 2 cut(s) 66, 279
Eco88I CYCGRG 1 cut(s) 617
EcoRII CCWGG 4 cut(s) 43, 51, 172, 523
EcoT22I ATGCAT 1 cut(s) 238
FaeI CATG 4 cut(s) 247, 514, 593, 696
FaiI YATR 9 cut(s) 63, 89, 104, 245, 345, 508, 512, 591, 694
FaqI GGGAC 1 cut(s) 417
FatI CATG 4 cut(s) 243, 510, 589, 692
FokI GGATG 4 cut(s) 68, 122, 226, 366
FspBI CTAG 1 cut(s) 129
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 2 cut(s) 486, 686
Hin1II CATG 4 cut(s) 247, 514, 593, 696
HinfI GANTC 2 cut(s) 461, 677
HphI GGTGA 4 cut(s) 296, 512, 610, 662
Hpy166II GTNNAC 3 cut(s) 41, 471, 493
Hpy188I TCNGA 4 cut(s) 85, 259, 267, 676
Hpy188III TCNNGA 3 cut(s) 383, 619, 693
Hpy8I GTNNAC 3 cut(s) 41, 471, 493
HpyAV CCTTC 2 cut(s) 331, 541
HpyCH4III ACNGT 2 cut(s) 205, 475
HpyCH4V TGCA 4 cut(s) 236, 311, 392, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
Hsp92II CATG 4 cut(s) 247, 514, 593, 696
LmnI GCTCC 1 cut(s) 179
LweI GCATC 3 cut(s) 223, 245, 277
MaeI CTAG 1 cut(s) 129
MboII GAAGA 1 cut(s) 672
MluCI AATT 2 cut(s) 496, 608
MnlI CCTC 4 cut(s) 140, 170, 411, 521
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 2 cut(s) 398, 441
MspR9I CCNGG 4 cut(s) 45, 53, 174, 525
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 4 cut(s) 45, 53, 174, 525
MwoI GCNNNNNNNGC 1 cut(s) 317
NlaIII CATG 4 cut(s) 247, 514, 593, 696
NlaIV GGNNCC 2 cut(s) 181, 687
NsiI ATGCAT 1 cut(s) 238
PaeR7I CTCGAG 1 cut(s) 617
PagI TCATGA 1 cut(s) 692
PctI GAATGC 1 cut(s) 607
PfeI GAWTC 2 cut(s) 461, 677
Psp6I CCWGG 4 cut(s) 43, 51, 172, 523
PspGI CCWGG 4 cut(s) 43, 51, 172, 523
PspN4I GGNNCC 2 cut(s) 181, 687
PspPI GGNCC 2 cut(s) 38, 685
RsaI GTAC 2 cut(s) 494, 637
RsaNI GTAC 2 cut(s) 493, 636
SaqAI TTAA 2 cut(s) 398, 441
Sau96I GGNCC 2 cut(s) 38, 685
ScrFI CCNGG 4 cut(s) 45, 53, 174, 525
SetI ASST 8 cut(s) 36, 78, 151, 166, 286, 390, 397, 532
SfaNI GCATC 3 cut(s) 223, 245, 277
Sfr274I CTCGAG 1 cut(s) 617
SinI GGWCC 1 cut(s) 38
SlaI CTCGAG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SpeI ACTAGT 1 cut(s) 128
Sse9I AATT 2 cut(s) 496, 608
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 4 cut(s) 43, 51, 172, 523
TaaI ACNGT 2 cut(s) 205, 475
TaqI TCGA 2 cut(s) 373, 618
TasI AATT 2 cut(s) 496, 608
TatI WGTACW 1 cut(s) 492
TfiI GAWTC 2 cut(s) 461, 677
Tru1I TTAA 2 cut(s) 398, 441
Tru9I TTAA 2 cut(s) 398, 441
TscAI CASTG 3 cut(s) 229, 306, 435
TspDTI ATGAA 2 cut(s) 260, 450
TspRI CASTG 3 cut(s) 229, 306, 435
VpaK11BI GGWCC 1 cut(s) 38
XapI RAATTY 1 cut(s) 608
XhoI CTCGAG 1 cut(s) 617
XspI CTAG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.