AT5G10460

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007953
Physical Location & Seq
Forward (+)
3287750 .. 3289948
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10460.1

Sequence Viewer

Length: 921 bp
ATGTTCTGTTCCTCGCAGCTCTCACAGCCAATGAATGGCCTCAAAGACATAGTTGAGACGAGAAATTTTAAGGCTTGGCTCTTAGATCAGTATGGAGTTCTTCATGATGGCAAAAAACCTTACCCCGGTGCTATCTCAACATTGAAAAATCTTGCAACAGCGGGTGCCAAAATTGTGATCATTAGTAATTCTTCTAGACGTGCTTCAACTACAATGGAAAAGTTGAAAGGCCTTGGCTTTGATCCTTCTTTCTTCACCGGAGCTATAACTAGTGGTGAACTAACTCATCAATCTTTGCAGAGGAGAGATGATCCTTGGTTTGCTGCACTGGGAAGGAGGTGCATTCACATTACTTGGAATGACCGAGGAGCAATCTCTTTGGAGGGTCTAGATCTAAATGTTGTGGAGAATGTGGAAGAAGCTGACTTTGTTTTAGCGCACGGCACTGAAGCCCTAGGACTTCCTTCAGGCAGTGTATCTCCAAGGACTATTGATGAGCTAGAGAAGATTTTGGAGAAATCTGCAGCTCGAGGACTTCCCATGATCGTAGCTAATCCAGATTACGTGACTGTTGAAGCAAATGTTTTTCATATCATGCCAGGTACACTAGCTTCCAAGTATGAAGAACTGGGAGGAGAAGTGAAGTCGATGGGAAAACCTCATAAGATGATCTATGAGTCTGCTATAGCAATTGCGGGAGTCAATCCGTCTGAGTCTATCGCAGTAGGAGATTCGCTACATCATGATATAAGGGGAGCAAATGTTTCAGGAATTGAATCAATATTCATCACTGGAGGGATTCATGGTAATGAGCTTGGTCTCACTTCTTTTGATGAAACTGCAAGTCTAGACTCAGTCAAGACCCTTACTGCTAAACACAATGCATTCCCAACTTATGTATTATCAGCCTTTAAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

306

Amino Acids

33.03

Weight (kDa)

5.81

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 26 - 104 1.6e-14 Haloacid dehalogenase-like hydrolase
Hydrolase PF00702 163 - 256 8.6e-08 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 216 - 262 7.5e-06 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 217 - 286 2.6e-13 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 35
AciI CCGC 2 cut(s) 161, 695
AclWI GGATC 2 cut(s) 236, 305
AcsI RAATTY 1 cut(s) 64
AcuI CTGAAG 2 cut(s) 450, 468
AfaI GTAC 1 cut(s) 604
AfiI CCNNNNNNNGG 3 cut(s) 35, 125, 915
AgsI TTSAA 5 cut(s) 145, 207, 226, 575, 776
AhlI ACTAGT 1 cut(s) 269
AjiI CACGTC 1 cut(s) 200
AjnI CCWGG 1 cut(s) 598
AluBI AGCT 8 cut(s) 19, 263, 422, 499, 527, 551, 611, 814
AluI AGCT 8 cut(s) 19, 263, 422, 499, 527, 551, 611, 814
Alw26I GTCTC 2 cut(s) 50, 824
AlwI GGATC 2 cut(s) 236, 305
Ama87I CYCGRG 1 cut(s) 528
AoxI GGCC 2 cut(s) 37, 229
ApeKI GCWGC 3 cut(s) 16, 323, 524
ApoI RAATTY 1 cut(s) 64
AspA2I CCTAGG 1 cut(s) 454
AspLEI GCGC 1 cut(s) 439
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 247, 287
AvaI CYCGRG 1 cut(s) 528
AvrII CCTAGG 1 cut(s) 454
BanI GGYRCC 1 cut(s) 164
BarI GAAGNNNNNNTAC 2 cut(s) 595, 627
BbvI GCAGC 3 cut(s) 28, 310, 536
BccI CCATC 2 cut(s) 101, 643
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 1 cut(s) 600
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 2 cut(s) 50, 824
BcuI ACTAGT 1 cut(s) 269
BfaI CTAG 7 cut(s) 195, 270, 389, 455, 500, 608, 848
BfmI CTRYAG 2 cut(s) 522, 684
BglII AGATCT 1 cut(s) 391
BisI GCNGC 3 cut(s) 17, 324, 525
BlnI CCTAGG 1 cut(s) 454
BlsI GCNGC 3 cut(s) 18, 325, 526
Bme1390I CCNGG 2 cut(s) 126, 600
BmeT110I CYCGRG 1 cut(s) 528
BmgBI CACGTC 1 cut(s) 200
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 2 cut(s) 126, 600
BmrI ACTGGG 2 cut(s) 338, 638
BmuI ACTGGG 2 cut(s) 338, 638
BpmI CTGGAG 1 cut(s) 813
BpuMI CCSGG 1 cut(s) 126
BsaAI YACGTR 1 cut(s) 565
BsaI GGTCTC 1 cut(s) 824
BsaJI CCNNGG 6 cut(s) 124, 232, 314, 364, 454, 482
BsaWI WCCGGW 1 cut(s) 257
BsaXI ACNNNNNCTCC 4 cut(s) 624, 654, 747, 777
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 125, 915
Bse1I ACTGG 3 cut(s) 333, 633, 796
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 6 cut(s) 124, 232, 314, 364, 454, 482
BseLI CCNNNNNNNGG 3 cut(s) 35, 125, 915
BseMII CTCAG 2 cut(s) 702, 867
BseNI ACTGG 3 cut(s) 333, 633, 796
BseRI GAGGAG 3 cut(s) 316, 381, 648
BseXI GCAGC 3 cut(s) 28, 310, 536
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 2 cut(s) 39, 231
BshNI GGYRCC 1 cut(s) 164
BsiHKCI CYCGRG 1 cut(s) 528
BsiSI CCGG 2 cut(s) 126, 258
BslI CCNNNNNNNGG 3 cut(s) 35, 125, 915
BsmAI GTCTC 2 cut(s) 50, 824
BsmBI CGTCTC 1 cut(s) 50
BsmI GAATGC 2 cut(s) 342, 884
BsnI GGCC 2 cut(s) 39, 231
Bso31I GGTCTC 1 cut(s) 824
BsoBI CYCGRG 1 cut(s) 528
Bsp143I GATC 7 cut(s) 85, 177, 241, 310, 391, 543, 669
BspACI CCGC 2 cut(s) 161, 695
BspANI GGCC 2 cut(s) 39, 231
BspCNI CTCAG 2 cut(s) 703, 866
BspHI TCATGA 2 cut(s) 103, 742
BspLI GGNNCC 1 cut(s) 166
BspMAI CTGCAG 1 cut(s) 526
BspPI GGATC 2 cut(s) 236, 305
BspT107I GGYRCC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 824
BsrI ACTGG 3 cut(s) 333, 633, 796
BssECI CCNNGG 6 cut(s) 124, 232, 314, 364, 454, 482
BssMI GATC 7 cut(s) 85, 177, 241, 310, 391, 543, 669
BssT1I CCWWGG 4 cut(s) 232, 314, 454, 482
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 1 cut(s) 571
BstBAI YACGTR 1 cut(s) 565
BstDEI CTNAG 3 cut(s) 82, 711, 853
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 7 cut(s) 88, 180, 244, 313, 394, 546, 672
BstMAI GTCTC 2 cut(s) 50, 824
BstMBI GATC 7 cut(s) 85, 177, 241, 310, 391, 543, 669
BstMWI GCNNNNNNNGC 1 cut(s) 25
BstNI CCWGG 1 cut(s) 600
BstSCI CCNGG 2 cut(s) 124, 598
BstSFI CTRYAG 2 cut(s) 522, 684
BstV1I GCAGC 3 cut(s) 28, 310, 536
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 2 cut(s) 39, 231
BtrI CACGTC 1 cut(s) 200
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 4 cut(s) 326, 444, 478, 789
CciI TCATGA 2 cut(s) 103, 742
CfoI GCGC 1 cut(s) 439
Csp6I GTAC 1 cut(s) 603
CviAII CATG 5 cut(s) 104, 541, 595, 743, 803
CviQI GTAC 1 cut(s) 603
DdeI CTNAG 3 cut(s) 82, 711, 853
DpnI GATC 7 cut(s) 87, 179, 243, 312, 393, 545, 671
DpnII GATC 7 cut(s) 85, 177, 241, 310, 391, 543, 669
DraI TTTAAA 1 cut(s) 913
Eco130I CCWWGG 4 cut(s) 232, 314, 454, 482
Eco147I AGGCCT 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 824
Eco57I CTGAAG 2 cut(s) 450, 468
Eco88I CYCGRG 1 cut(s) 528
EcoRII CCWGG 1 cut(s) 598
EcoT14I CCWWGG 4 cut(s) 232, 314, 454, 482
EcoT22I ATGCAT 1 cut(s) 886
ErhI CCWWGG 4 cut(s) 232, 314, 454, 482
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 5 cut(s) 107, 544, 598, 746, 806
FatI CATG 5 cut(s) 103, 540, 594, 742, 802
FauI CCCGC 2 cut(s) 154, 688
FbaI TGATCA 1 cut(s) 177
Fnu4HI GCNGC 3 cut(s) 17, 324, 525
Fsp4HI GCNGC 3 cut(s) 17, 324, 525
FspBI CTAG 7 cut(s) 195, 270, 389, 455, 500, 608, 848
GlaI GCGC 1 cut(s) 438
GluI GCNGC 3 cut(s) 17, 324, 525
GsuI CTGGAG 1 cut(s) 813
HaeIII GGCC 2 cut(s) 39, 231
HapII CCGG 2 cut(s) 126, 258
HhaI GCGC 1 cut(s) 439
Hin1II CATG 5 cut(s) 107, 544, 598, 746, 806
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HinfI GANTC 7 cut(s) 677, 699, 713, 731, 776, 799, 851
HpaII CCGG 2 cut(s) 126, 258
HphI GGTGA 2 cut(s) 247, 287
Hpy166II GTNNAC 2 cut(s) 278, 605
Hpy188I TCNGA 1 cut(s) 712
Hpy188III TCNNGA 8 cut(s) 104, 195, 389, 557, 743, 768, 848, 859
Hpy8I GTNNAC 2 cut(s) 278, 605
HpyAV CCTTC 3 cut(s) 255, 327, 474
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4IV ACGT 2 cut(s) 199, 564
HpyCH4V TGCA 7 cut(s) 155, 298, 326, 342, 524, 842, 884
HpyF10VI GCNNNNNNNGC 1 cut(s) 25
HpyF3I CTNAG 3 cut(s) 82, 711, 853
HpySE526I ACGT 2 cut(s) 199, 564
Hsp92II CATG 5 cut(s) 107, 544, 598, 746, 806
HspAI GCGC 1 cut(s) 437
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 7 cut(s) 85, 177, 241, 310, 391, 543, 669
LmnI GCTCC 3 cut(s) 260, 368, 755
Lsp1109I GCAGC 3 cut(s) 28, 310, 536
MaeI CTAG 7 cut(s) 195, 270, 389, 455, 500, 608, 848
MaeII ACGT 2 cut(s) 199, 564
MaeIII GTNAC 1 cut(s) 565
MalI GATC 7 cut(s) 87, 179, 243, 312, 393, 545, 671
MboI GATC 7 cut(s) 85, 177, 241, 310, 391, 543, 669
MboII GAAGA 6 cut(s) 92, 183, 244, 428, 517, 635
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 1 cut(s) 391
MluCI AATT 5 cut(s) 64, 171, 187, 690, 771
MlyI GAGTC 4 cut(s) 686, 708, 722, 845
Mph1103I ATGCAT 1 cut(s) 886
MseI TTAA 2 cut(s) 69, 912
MslI CAYNNNNRTG 1 cut(s) 807
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 126, 258
MspR9I CCNGG 2 cut(s) 126, 600
MunI CAATTG 1 cut(s) 690
Mva1269I GAATGC 2 cut(s) 342, 884
MvaI CCWGG 1 cut(s) 600
MwoI GCNNNNNNNGC 1 cut(s) 25
NciI CCSGG 1 cut(s) 126
NdeII GATC 7 cut(s) 85, 177, 241, 310, 391, 543, 669
NlaIII CATG 5 cut(s) 107, 544, 598, 746, 806
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 565
NsiI ATGCAT 1 cut(s) 886
PaeR7I CTCGAG 1 cut(s) 528
PagI TCATGA 2 cut(s) 103, 742
PceI AGGCCT 1 cut(s) 231
PctI GAATGC 2 cut(s) 342, 884
PfeI GAWTC 3 cut(s) 731, 776, 799
PflFI GACNNNGTC 1 cut(s) 854
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 3 cut(s) 18, 325, 526
PleI GAGTC 4 cut(s) 685, 707, 721, 845
PpsI GAGTC 4 cut(s) 685, 707, 721, 845
Ppu21I YACGTR 1 cut(s) 565
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 1 cut(s) 166
PspXI VCTCGAGB 1 cut(s) 528
PstI CTGCAG 1 cut(s) 526
PsuI RGATCY 1 cut(s) 391
PsyI GACNNNGTC 1 cut(s) 854
RsaI GTAC 1 cut(s) 604
RsaNI GTAC 1 cut(s) 603
RseI CAYNNNNRTG 1 cut(s) 807
SaqAI TTAA 2 cut(s) 69, 912
SatI GCNGC 3 cut(s) 17, 324, 525
Sau3AI GATC 7 cut(s) 85, 177, 241, 310, 391, 543, 669
SchI GAGTC 4 cut(s) 686, 708, 722, 845
ScrFI CCNGG 2 cut(s) 126, 600
SfcI CTRYAG 2 cut(s) 522, 684
Sfr274I CTCGAG 1 cut(s) 528
SlaI CTCGAG 1 cut(s) 528
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
SpeI ACTAGT 1 cut(s) 269
Sse9I AATT 5 cut(s) 64, 171, 187, 690, 771
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 2 cut(s) 161, 695
SspI AATATT 1 cut(s) 783
SspMI CTAG 7 cut(s) 195, 270, 389, 455, 500, 608, 848
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 124, 598
StyI CCWWGG 4 cut(s) 232, 314, 454, 482
TaaI ACNGT 1 cut(s) 571
TaiI ACGT 2 cut(s) 202, 567
TaqI TCGA 2 cut(s) 529, 647
TaqII GACCGA 1 cut(s) 378
TasI AATT 5 cut(s) 64, 171, 187, 690, 771
TfiI GAWTC 3 cut(s) 731, 776, 799
Tru1I TTAA 2 cut(s) 69, 912
Tru9I TTAA 2 cut(s) 69, 912
TscAI CASTG 4 cut(s) 333, 451, 478, 796
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 3 cut(s) 16, 323, 524
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 7 cut(s) 47, 92, 578, 636, 775, 791, 849
TspGWI ACGGA 1 cut(s) 696
TspRI CASTG 4 cut(s) 333, 451, 478, 796
Tth111I GACNNNGTC 1 cut(s) 854
Van91I CCANNNNNTGG 1 cut(s) 35
XapI RAATTY 1 cut(s) 64
XbaI TCTAGA 3 cut(s) 194, 388, 847
XhoI CTCGAG 1 cut(s) 528
XmaJI CCTAGG 1 cut(s) 454
XspI CTAG 7 cut(s) 195, 270, 389, 455, 500, 608, 848
Zsp2I ATGCAT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.