Rh2AG005900

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007953
Physical Location & Seq
Reverse (-)
547395 .. 551257
3863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG005900.1

Sequence Viewer

Length: 696 bp
ATGATGCCCAGATGCTCCGTTCAATCCTCAGATGTTCAGCTCTTTCAGACCATAACTGGCCTTCAACAAATCGCCGAAACCCATCGTTTCAAGGCCTGGTTATTAGATCAGTTTGGAGTACTCCACGATGGGAAGCAGCCTTATCCCGGAGCAATTTCAACATTGAAGAAGCTAGCAAATAGTGGTGCAAAGATGGTTATTATAAGTAATTCATCCAGACGCTCGTCTACGACCTTGGAGAAATTGAGCAGCCTTGGTTTTGATCCTTCTCTCTTTGTAGGAGCCATCACTAGCGGGGAATTAACACATCAGAGCTTGCAAAGGAGAGATGATGCTTGGTTTGCAGCACTGGGAAAATCTTGCATTCATATGACTTGGAGTGCTCGTGGTGCCATATCTCTTGAGGGCCTTGGATTACAAGTTGTGGAAAATGCTCAAGATGCTGACTTTATTTTGGCTCATGGCACTGAAGCCTTGGGGAATCCTTCAGGTGATGCAATTCCCATGAAACTGGAGGAACTTGAGAATATATTGGAACAATGTGCTGCTAAGCACATTCCTATGGTGGTGGCCAATCCCGATTTTGTGACTGTTGAGGCTAAAGCTTTGCGTGTGATGCCAGGGACTCTGGCTGCCAAATATGAAGAGCTGGGAGGTGAAGTGAAATGGATGGGCAAGCCTGATAAGGTGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

231

Amino Acids

25.03

Weight (kDa)

6.07

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 33 - 119 2.1e-15 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 227
AciI CCGC 1 cut(s) 294
AclWI GGATC 1 cut(s) 257
AcoI YGGCCR 1 cut(s) 570
AcuI CTGAAG 2 cut(s) 471, 489
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 5 cut(s) 23, 65, 91, 159, 166
AjnI CCWGG 2 cut(s) 95, 619
AluBI AGCT 5 cut(s) 40, 172, 315, 605, 649
AluI AGCT 5 cut(s) 40, 172, 315, 605, 649
Alw21I GWGCWC 1 cut(s) 385
AlwI GGATC 1 cut(s) 257
AoxI GGCC 4 cut(s) 58, 93, 406, 570
ApeKI GCWGC 5 cut(s) 136, 249, 344, 545, 632
AspS9I GGNCC 1 cut(s) 406
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 503, 668
AsuNHI GCTAGC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 572
BanI GGYRCC 1 cut(s) 389
BauI CACGAG 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 385
BbvI GCAGC 5 cut(s) 148, 261, 356, 532, 619
BccI CCATC 5 cut(s) 90, 122, 187, 293, 664
BciT130I CCWGG 2 cut(s) 97, 621
BcnI CCSGG 1 cut(s) 147
BfaI CTAG 2 cut(s) 173, 291
BisI GCNGC 5 cut(s) 137, 250, 345, 546, 633
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 5 cut(s) 138, 251, 346, 547, 634
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 3 cut(s) 97, 147, 621
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 2 cut(s) 283, 391
BmrFI CCNGG 3 cut(s) 97, 147, 621
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 5 cut(s) 2, 322, 430, 484, 606
BmtI GCTAGC 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 359
BoxI GACNNNNGTC 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 533
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 3 cut(s) 420, 422, 542
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 5 cut(s) 234, 253, 409, 474, 620
BsaXI ACNNNNNCTCC 4 cut(s) 370, 400, 645, 675
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse1I ACTGG 3 cut(s) 61, 354, 516
BseBI CCWGG 2 cut(s) 97, 621
BseDI CCNNGG 5 cut(s) 234, 253, 409, 474, 620
BseGI GGATG 2 cut(s) 212, 675
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 3 cut(s) 61, 354, 516
BseXI GCAGC 5 cut(s) 148, 261, 356, 532, 619
BseYI CCCAGC 1 cut(s) 649
BshFI GGCC 4 cut(s) 60, 95, 408, 572
BshNI GGYRCC 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 385
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 4 cut(s) 60, 95, 408, 572
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 2 cut(s) 106, 262
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 1 cut(s) 294
BspANI GGCC 4 cut(s) 60, 95, 408, 572
BspCNI CTCAG 1 cut(s) 41
BspLI GGNNCC 2 cut(s) 283, 391
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 389
BsrI ACTGG 3 cut(s) 61, 354, 516
BssECI CCNNGG 5 cut(s) 234, 253, 409, 474, 620
BssMI GATC 2 cut(s) 106, 262
BssSI CACGAG 1 cut(s) 384
BssT1I CCWWGG 4 cut(s) 234, 253, 409, 474
Bst2BI CACGAG 1 cut(s) 384
Bst2UI CCWGG 2 cut(s) 97, 621
Bst4CI ACNGT 1 cut(s) 592
Bst6I CTCTTC 1 cut(s) 639
BstC8I GCNNGC 3 cut(s) 174, 317, 677
BstDEI CTNAG 2 cut(s) 28, 549
BstF5I GGATG 2 cut(s) 212, 675
BstKTI GATC 2 cut(s) 109, 265
BstMBI GATC 2 cut(s) 106, 262
BstMWI GCNNNNNNNGC 4 cut(s) 341, 389, 440, 616
BstNI CCWGG 2 cut(s) 97, 621
BstPAI GACNNNNGTC 1 cut(s) 223
BstSCI CCNGG 3 cut(s) 95, 145, 619
BstV1I GCAGC 5 cut(s) 148, 261, 356, 532, 619
BstXI CCANNNNNNTGG 1 cut(s) 511
BsuRI GGCC 4 cut(s) 60, 95, 408, 572
BtsCI GGATG 2 cut(s) 212, 675
BtsIMutI CAGTG 2 cut(s) 347, 465
Cac8I GCNNGC 3 cut(s) 174, 317, 677
Cfr13I GGNCC 1 cut(s) 406
CseI GACGC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 119
CviAII CATG 2 cut(s) 461, 505
CviQI GTAC 1 cut(s) 119
DdeI CTNAG 2 cut(s) 28, 549
DpnI GATC 2 cut(s) 108, 264
DpnII GATC 2 cut(s) 106, 262
EaeI YGGCCR 1 cut(s) 570
Eam1104I CTCTTC 1 cut(s) 639
EarI CTCTTC 1 cut(s) 639
Eco130I CCWWGG 4 cut(s) 234, 253, 409, 474
Eco147I AGGCCT 1 cut(s) 95
Eco57I CTGAAG 2 cut(s) 471, 489
EcoO109I RGGNCCY 1 cut(s) 406
EcoRII CCWGG 2 cut(s) 95, 619
EcoT14I CCWWGG 4 cut(s) 234, 253, 409, 474
ErhI CCWWGG 4 cut(s) 234, 253, 409, 474
FaeI CATG 2 cut(s) 464, 508
FaqI GGGAC 1 cut(s) 637
FatI CATG 2 cut(s) 460, 504
FauI CCCGC 1 cut(s) 287
FauNDI CATATG 1 cut(s) 369
FblI GTMKAC 1 cut(s) 227
Fnu4HI GCNGC 5 cut(s) 137, 250, 345, 546, 633
FokI GGATG 2 cut(s) 199, 682
Fsp4HI GCNGC 5 cut(s) 137, 250, 345, 546, 633
FspBI CTAG 2 cut(s) 173, 291
GluI GCNGC 5 cut(s) 137, 250, 345, 546, 633
GsaI CCCAGC 1 cut(s) 653
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 4 cut(s) 60, 95, 408, 572
HapII CCGG 1 cut(s) 147
HgaI GACGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 464, 508
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 2 cut(s) 481, 625
HpaII CCGG 1 cut(s) 147
HphI GGTGA 2 cut(s) 503, 668
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 3 cut(s) 31, 48, 312
Hpy188III TCNNGA 4 cut(s) 216, 401, 437, 578
Hpy8I GTNNAC 1 cut(s) 228
HpyAV CCTTC 3 cut(s) 71, 276, 495
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 5 cut(s) 188, 319, 344, 363, 497
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 389, 440, 616
HpyF3I CTNAG 2 cut(s) 28, 549
Hsp92II CATG 2 cut(s) 464, 508
Kzo9I GATC 2 cut(s) 106, 262
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 3 cut(s) 20, 149, 281
Lsp1109I GCAGC 5 cut(s) 148, 261, 356, 532, 619
LweI GCATC 5 cut(s) 2, 322, 430, 484, 606
MaeI CTAG 2 cut(s) 173, 291
MaeIII GTNAC 1 cut(s) 586
MalI GATC 2 cut(s) 108, 264
MboI GATC 2 cut(s) 106, 262
MboII GAAGA 2 cut(s) 178, 656
MhlI GDGCHC 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 5 cut(s) 153, 208, 242, 299, 498
MluNI TGGCCA 1 cut(s) 572
MlyI GAGTC 1 cut(s) 619
MnlI CCTC 5 cut(s) 37, 397, 508, 589, 647
Mox20I TGGCCA 1 cut(s) 572
MscI TGGCCA 1 cut(s) 572
MseI TTAA 1 cut(s) 302
MslI CAYNNNNRTG 2 cut(s) 368, 560
Msp20I TGGCCA 1 cut(s) 572
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 3 cut(s) 97, 147, 621
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 2 cut(s) 97, 621
MwoI GCNNNNNNNGC 4 cut(s) 341, 389, 440, 616
NciI CCSGG 1 cut(s) 147
NdeI CATATG 1 cut(s) 369
NdeII GATC 2 cut(s) 106, 262
NheI GCTAGC 1 cut(s) 172
NlaIII CATG 2 cut(s) 464, 508
NlaIV GGNNCC 2 cut(s) 283, 391
NmuCI GTSAC 1 cut(s) 586
PceI AGGCCT 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 639
PctI GAATGC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 481
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 5 cut(s) 138, 251, 346, 547, 634
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PshAI GACNNNNGTC 1 cut(s) 223
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 95, 619
PspFI CCCAGC 1 cut(s) 649
PspGI CCWGG 2 cut(s) 95, 619
PspN4I GGNNCC 2 cut(s) 283, 391
PspPI GGNCC 1 cut(s) 406
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
RseI CAYNNNNRTG 2 cut(s) 368, 560
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 5 cut(s) 137, 250, 345, 546, 633
Sau3AI GATC 2 cut(s) 106, 262
Sau96I GGNCC 1 cut(s) 406
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 3 cut(s) 97, 147, 621
SduI GDGCHC 1 cut(s) 385
SetI ASST 9 cut(s) 42, 174, 236, 317, 493, 607, 651, 658, 690
SfaNI GCATC 5 cut(s) 2, 322, 430, 484, 606
SmiMI CAYNNNNRTG 2 cut(s) 368, 560
SmlI CTYRAG 3 cut(s) 401, 435, 521
SmoI CTYRAG 3 cut(s) 401, 435, 521
Sse9I AATT 5 cut(s) 153, 208, 242, 299, 498
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 1 cut(s) 294
SspMI CTAG 2 cut(s) 173, 291
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 3 cut(s) 95, 145, 619
StyI CCWWGG 4 cut(s) 234, 253, 409, 474
TaaI ACNGT 1 cut(s) 592
TasI AATT 5 cut(s) 153, 208, 242, 299, 498
TatI WGTACW 1 cut(s) 118
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TscAI CASTG 2 cut(s) 354, 472
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 5 cut(s) 136, 249, 344, 545, 632
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 4 cut(s) 201, 356, 521, 657
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 2 cut(s) 354, 472
XmiI GTMKAC 1 cut(s) 227
XspI CTAG 2 cut(s) 173, 291
ZrmI AGTACT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.