MD15G1148400.v1.1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007953
Physical Location & Seq
Forward (+)
10982014 .. 10984802
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1148400.v1.1.491

Sequence Viewer

Length: 921 bp
ATGAGGGGAATGAACAGTTTGTATGATAAAACTATGCCGTGTCACCATTGGAGGAGGAGTGTTTTCTTACATGCCTGGTTCTTGGATCAGTTTGGAGTTCTCCATGATGGGAAGCAACCTTACCCTGGTGCAATTTCAACATTGAAGAAGCTAGCAAGCAGTGGTGCAAAGATGGTTATTATAAGTAATTCATCAAGACGCTCGTCTACAACCATGGATAAATTGAAAAGCCTCGGGTTCGATCCTTCTCTTTTTGTAGGAGCCATCACTAGCGGAGAACTGACACATCAAAGCTTACAAAGGAGAGATGATGCTTGGTTCGCTGCACTGGGAAAATCTTGTATTCATATGACTTGGAGTGCTCGTGGTACCATATCTCTTGAGGGCCTGGGACTACAAGTTGTGGAGAAAGTTCAAGACGCCGAATTTATTTTGGCCCATGGCTCTGAAGCCTTGGGGAGTCCTTCTGGTGATGCGATTCCAATGAAACTTGAGGAACTTGAAAGTATATTGGAACAATGTGCTGCTAAACACATCCCTATGGTGGTAGCAAATCCAGATTTTGTGACTGTTGAGGCTAGAGCTTTGCGTGTAATGCCTGGGACTTTGGCTTCCAAGTATGAAAAGCTTGGGGGTGAAGTGAAATGTATGGGCAAGCCTGATAAGATCATCTACAAATCAGCTATGGACTTAGCTGGTGTAGATCCTGCTGAATCTATCGCAGTTGGCGATTCTCTTCACCACGACATCAAGGGTGCAAATGCAGCTGCAATCCAATCAGTTTTTGTCACCTGTGGGATCCATGGAAATGAACTTGGACTCAATAGCTTCGCGGACGTTGCAGATTTATCTTCTGTGCAAGCTCTTGCTTCCAAATACGATGCGTACCCGTCATACGTGTTACCTTCATTCACATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

307

Amino Acids

33.04

Weight (kDa)

6.41

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 26 - 105 4.7e-15 Haloacid dehalogenase-like hydrolase
Hydrolase PF00702 166 - 256 8.4e-07 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 217 - 272 5e-13 HAD-hyrolase-like
HAD_2 PF13419 218 - 263 4.3e-06 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 182
Acc65I GGTACC 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 368
AccI GTMKAC 1 cut(s) 206
AccII CGCG 1 cut(s) 833
AciI CCGC 2 cut(s) 273, 833
AclWI GGATC 5 cut(s) 93, 236, 698, 793, 806
AcsI RAATTY 1 cut(s) 425
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 2 cut(s) 370, 887
AfiI CCNNNNNNNGG 2 cut(s) 125, 544
AflIII ACRYGT 1 cut(s) 897
AgsI TTSAA 5 cut(s) 138, 145, 226, 416, 503
AjnI CCWGG 4 cut(s) 74, 124, 387, 598
AluBI AGCT 9 cut(s) 151, 294, 584, 628, 683, 695, 767, 828, 863
AluI AGCT 9 cut(s) 151, 294, 584, 628, 683, 695, 767, 828, 863
Alw21I GWGCWC 1 cut(s) 364
AlwI GGATC 5 cut(s) 93, 236, 698, 793, 806
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 2 cut(s) 385, 435
ApeKI GCWGC 4 cut(s) 323, 524, 764, 767
ApoI RAATTY 1 cut(s) 425
Asp718I GGTACC 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 385, 436
AsuHPI GGTGA 5 cut(s) 35, 482, 647, 731, 781
AsuNHI GCTAGC 1 cut(s) 151
AvaI CYCGRG 1 cut(s) 233
BamHI GGATCC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 368
BarI GAAGNNNNNNTAC 2 cut(s) 104, 136
BauI CACGAG 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 4 cut(s) 310, 511, 754, 776
BccI CCATC 3 cut(s) 101, 166, 272
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 4 cut(s) 76, 126, 389, 600
BfaI CTAG 3 cut(s) 152, 270, 579
BisI GCNGC 4 cut(s) 324, 525, 765, 768
BlsI GCNGC 4 cut(s) 325, 526, 766, 769
Bme1390I CCNGG 4 cut(s) 76, 126, 389, 600
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 2 cut(s) 385, 436
BmiI GGNNCC 3 cut(s) 262, 370, 800
BmrFI CCNGG 4 cut(s) 76, 126, 389, 600
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 3 cut(s) 301, 463, 871
BmtI GCTAGC 1 cut(s) 155
BmuI ACTGGG 1 cut(s) 338
BoxI GACNNNNGTC 1 cut(s) 202
BpuEI CTTGAG 2 cut(s) 401, 512
BsaAI YACGTR 1 cut(s) 898
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 8 cut(s) 124, 213, 232, 388, 439, 453, 599, 802
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 349, 379
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 544
Bse1I ACTGG 1 cut(s) 333
BseBI CCWGG 4 cut(s) 76, 126, 389, 600
BseDI CCNNGG 8 cut(s) 124, 213, 232, 388, 439, 453, 599, 802
BseGI GGATG 1 cut(s) 534
BseLI CCNNNNNNNGG 2 cut(s) 125, 544
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 2 cut(s) 67, 70
BseXI GCAGC 4 cut(s) 310, 511, 754, 776
BsgI GTGCAG 1 cut(s) 309
Bsh1236I CGCG 1 cut(s) 833
BshFI GGCC 2 cut(s) 387, 437
BshNI GGYRCC 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 364
BsiHKCI CYCGRG 1 cut(s) 233
BslFI GGGAC 2 cut(s) 405, 616
BslI CCNNNNNNNGG 2 cut(s) 125, 544
BsmFI GGGAC 2 cut(s) 405, 616
BsnI GGCC 2 cut(s) 387, 437
BsoBI CYCGRG 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 5 cut(s) 85, 241, 666, 703, 798
Bsp19I CCATGG 3 cut(s) 213, 439, 802
BspACI CCGC 2 cut(s) 273, 833
BspANI GGCC 2 cut(s) 387, 437
BspFNI CGCG 1 cut(s) 833
BspLI GGNNCC 3 cut(s) 262, 370, 800
BspOI GCTAGC 1 cut(s) 155
BspPI GGATC 5 cut(s) 93, 236, 698, 793, 806
BspT107I GGYRCC 1 cut(s) 368
BsrI ACTGG 1 cut(s) 333
BssECI CCNNGG 8 cut(s) 124, 213, 232, 388, 439, 453, 599, 802
BssMI GATC 5 cut(s) 85, 241, 666, 703, 798
BssNI GRCGYC 1 cut(s) 420
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 4 cut(s) 213, 439, 453, 802
Bst2BI CACGAG 1 cut(s) 363
Bst2UI CCWGG 4 cut(s) 76, 126, 389, 600
Bst4CI ACNGT 2 cut(s) 17, 571
Bst6I CTCTTC 1 cut(s) 741
BstACI GRCGYC 1 cut(s) 420
BstBAI YACGTR 1 cut(s) 898
BstC8I GCNNGC 4 cut(s) 153, 157, 656, 861
BstDEI CTNAG 1 cut(s) 691
BstDSI CCRYGG 3 cut(s) 213, 439, 802
BstF5I GGATG 1 cut(s) 534
BstFNI CGCG 1 cut(s) 833
BstKTI GATC 5 cut(s) 88, 244, 669, 706, 801
BstMBI GATC 5 cut(s) 85, 241, 666, 703, 798
BstMWI GCNNNNNNNGC 4 cut(s) 320, 595, 764, 839
BstNI CCWGG 4 cut(s) 76, 126, 389, 600
BstNSI RCATGY 1 cut(s) 74
BstPAI GACNNNNGTC 1 cut(s) 202
BstSCI CCNGG 4 cut(s) 74, 124, 387, 598
BstUI CGCG 1 cut(s) 833
BstV1I GCAGC 4 cut(s) 310, 511, 754, 776
BstX2I RGATCY 2 cut(s) 703, 798
BstYI RGATCY 2 cut(s) 703, 798
BsuRI GGCC 2 cut(s) 387, 437
BtgI CCRYGG 3 cut(s) 213, 439, 802
BtsCI GGATG 1 cut(s) 534
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 166, 326
Cac8I GCNNGC 4 cut(s) 153, 157, 656, 861
Cfr13I GGNCC 2 cut(s) 385, 436
CseI GACGC 2 cut(s) 207, 428
Csp6I GTAC 2 cut(s) 369, 886
CviAII CATG 6 cut(s) 71, 104, 214, 440, 803, 915
CviQI GTAC 2 cut(s) 369, 886
DdeI CTNAG 1 cut(s) 691
DpnI GATC 5 cut(s) 87, 243, 668, 705, 800
DpnII GATC 5 cut(s) 85, 241, 666, 703, 798
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
Eco130I CCWWGG 4 cut(s) 213, 439, 453, 802
Eco57I CTGAAG 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 385
EcoRII CCWGG 4 cut(s) 74, 124, 387, 598
EcoT14I CCWWGG 4 cut(s) 213, 439, 453, 802
ErhI CCWWGG 4 cut(s) 213, 439, 453, 802
FaeI CATG 6 cut(s) 74, 107, 217, 443, 806, 918
FaqI GGGAC 2 cut(s) 405, 616
FatI CATG 6 cut(s) 70, 103, 213, 439, 802, 914
FauNDI CATATG 1 cut(s) 348
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 4 cut(s) 324, 525, 765, 768
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 4 cut(s) 324, 525, 765, 768
FspBI CTAG 3 cut(s) 152, 270, 579
GluI GCNGC 4 cut(s) 324, 525, 765, 768
HaeIII GGCC 2 cut(s) 387, 437
HgaI GACGC 2 cut(s) 207, 428
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 6 cut(s) 74, 107, 217, 443, 806, 918
HindIII AAGCTT 2 cut(s) 292, 626
HinfI GANTC 5 cut(s) 460, 478, 713, 731, 819
HphI GGTGA 5 cut(s) 35, 482, 647, 731, 781
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 1 cut(s) 448
Hpy188III TCNNGA 4 cut(s) 195, 380, 416, 557
Hpy8I GTNNAC 1 cut(s) 207
HpyAV CCTTC 3 cut(s) 255, 474, 915
HpyCH4III ACNGT 2 cut(s) 17, 571
HpyCH4IV ACGT 2 cut(s) 837, 897
HpyCH4V TGCA 8 cut(s) 131, 167, 326, 758, 764, 770, 842, 859
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 595, 764, 839
HpyF3I CTNAG 1 cut(s) 691
HpySE526I ACGT 2 cut(s) 837, 897
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 6 cut(s) 74, 107, 217, 443, 806, 918
KpnI GGTACC 1 cut(s) 372
Kzo9I GATC 5 cut(s) 85, 241, 666, 703, 798
LmnI GCTCC 1 cut(s) 260
Lsp1109I GCAGC 4 cut(s) 310, 511, 754, 776
LweI GCATC 3 cut(s) 301, 463, 871
MaeI CTAG 3 cut(s) 152, 270, 579
MaeII ACGT 2 cut(s) 837, 897
MaeIII GTNAC 4 cut(s) 41, 565, 787, 900
MalI GATC 5 cut(s) 87, 243, 668, 705, 800
MboI GATC 5 cut(s) 85, 241, 666, 703, 798
MboII GAAGA 3 cut(s) 157, 728, 843
MflI RGATCY 2 cut(s) 703, 798
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 4 cut(s) 132, 187, 221, 425
MlyI GAGTC 2 cut(s) 469, 813
MnlI CCTC 6 cut(s) 45, 48, 242, 376, 487, 568
MslI CAYNNNNRTG 3 cut(s) 539, 807, 913
MspA1I CMGCKG 1 cut(s) 767
MspR9I CCNGG 4 cut(s) 76, 126, 389, 600
MvaI CCWGG 4 cut(s) 76, 126, 389, 600
MvnI CGCG 1 cut(s) 833
MwoI GCNNNNNNNGC 4 cut(s) 320, 595, 764, 839
NcoI CCATGG 3 cut(s) 213, 439, 802
NdeI CATATG 1 cut(s) 348
NdeII GATC 5 cut(s) 85, 241, 666, 703, 798
NheI GCTAGC 1 cut(s) 151
NlaIII CATG 6 cut(s) 74, 107, 217, 443, 806, 918
NlaIV GGNNCC 3 cut(s) 262, 370, 800
NmuCI GTSAC 3 cut(s) 41, 565, 787
NspI RCATGY 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 726
PfeI GAWTC 3 cut(s) 478, 713, 731
PkrI GCNGC 4 cut(s) 325, 526, 766, 769
PleI GAGTC 2 cut(s) 468, 813
PpsI GAGTC 2 cut(s) 468, 813
Ppu21I YACGTR 1 cut(s) 898
PshAI GACNNNNGTC 1 cut(s) 202
PsiI TTATAA 1 cut(s) 182
Psp6I CCWGG 4 cut(s) 74, 124, 387, 598
PspGI CCWGG 4 cut(s) 74, 124, 387, 598
PspN4I GGNNCC 3 cut(s) 262, 370, 800
PspPI GGNCC 2 cut(s) 385, 436
PsuI RGATCY 2 cut(s) 703, 798
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 2 cut(s) 370, 887
RsaNI GTAC 2 cut(s) 369, 886
RseI CAYNNNNRTG 3 cut(s) 539, 807, 913
SatI GCNGC 4 cut(s) 324, 525, 765, 768
Sau3AI GATC 5 cut(s) 85, 241, 666, 703, 798
Sau96I GGNCC 2 cut(s) 385, 436
SchI GAGTC 2 cut(s) 469, 813
ScrFI CCNGG 4 cut(s) 76, 126, 389, 600
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 3 cut(s) 301, 463, 871
SmiMI CAYNNNNRTG 3 cut(s) 539, 807, 913
SmlI CTYRAG 2 cut(s) 380, 491
SmoI CTYRAG 2 cut(s) 380, 491
Sse9I AATT 4 cut(s) 132, 187, 221, 425
SsiI CCGC 2 cut(s) 273, 833
SspMI CTAG 3 cut(s) 152, 270, 579
StyD4I CCNGG 4 cut(s) 74, 124, 387, 598
StyI CCWWGG 4 cut(s) 213, 439, 453, 802
TaaI ACNGT 2 cut(s) 17, 571
TaiI ACGT 2 cut(s) 840, 900
TaqI TCGA 1 cut(s) 240
TasI AATT 4 cut(s) 132, 187, 221, 425
TfiI GAWTC 3 cut(s) 478, 713, 731
TscAI CASTG 2 cut(s) 166, 333
TseFI GTSAC 3 cut(s) 41, 565, 787
TseI GCWGC 4 cut(s) 323, 524, 764, 767
Tsp45I GTSAC 3 cut(s) 41, 565, 787
TspDTI ATGAA 7 cut(s) 26, 180, 335, 500, 636, 825, 897
TspRI CASTG 2 cut(s) 166, 333
XapI RAATTY 1 cut(s) 425
XceI RCATGY 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 206
XspI CTAG 3 cut(s) 152, 270, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.