RchiOBHm_Chr2g0085091

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007953
Physical Location & Seq
Reverse (-)
677003 .. 679455
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46065

Sequence Viewer

Length: 942 bp
ATGATGCCCAGATGCTCCGTTCAATCCTCAGATGTTCAGCTCTTTCAGACCATAACTGGCCTTCAACAACTCGCGGAAACCCATCGTTTCAAGGCCTGGTTATTAGATCAGTTTGGAGTACTTCACGATGGGAAGCAGCCTTATCCCGGAGCAATTTCAACATTGAAGAAGCTAGCAAATAGTGGTGCAAAGATGGTTATTATAAGTAATTCATCTAGACGCTCGTCTACGACCTTGGAGAAATTGAGCAGCCTTGGGTTTGATCCTTCTCTCTTTGTGGGAGCCATCACTAGCGGAGAATTAACACATCAGAGCTTGCAAAGGAGAGATGATGCTTGGTTTGCAGCACTGGGAAAATCTTGCATTCATATGACTTGGAGTGCTCGTGGTGCCATATCTCTTGAGGGCCTTGGATTACAAGTTGTGGAAAATGTTCAAGATGCTGACTTTATTTTGGCTCATGGCACTGAAGCCTTGGGGAATCCTTCAGGTGATGCAATTCCCATGAAACTGGAGGAACTTGAGAATATATTGGAACAATGTGCTGCTAAGCACATTCCTATGGTGGTGGCCAATCCTGATTTTGTGACTGTTGAGGCTAGAGCTTTGCGTGTGATGCCAGGGACTCTGGCTGCCAAATATGAAGAGCTGGGGGGTGAAGTGAAATGGATGGGCAAGCCTGATAAGATTATCTATAAATCAGCCATGGCCTTAGTTGGTGTAGACCCTATTGATTCTATTGCAGTGGGCGATTCTCTTCATCATGACATCAAGGGTGCAAATGCAGCTGCAATCCAATCAGTTTTAATCACTGGTGGAATCCATGCAACTGAACTTGGACTTGGTAGTTTTGCAGACGTAGCAGATTTACCTTCTGTGCAAGCTCTTGCTTCCAAATATGATGCTTACCCATCGTATGTGTTACCTGCCTTTACATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

313

Amino Acids

33.53

Weight (kDa)

5.57

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 33 - 119 4e-15 Haloacid dehalogenase-like hydrolase
Hydrolase PF00702 173 - 263 6.8e-06 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 224 - 278 2.4e-12 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 934
AccB1I GGYRCC 1 cut(s) 389
AccI GTMKAC 2 cut(s) 227, 723
AccII CGCG 1 cut(s) 74
AciI CCGC 2 cut(s) 74, 294
AclWI GGATC 1 cut(s) 257
AcoI YGGCCR 1 cut(s) 570
AcuI CTGAAG 2 cut(s) 471, 489
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 2 cut(s) 146, 936
AgsI TTSAA 6 cut(s) 23, 65, 91, 159, 166, 437
AjnI CCWGG 2 cut(s) 95, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 825, 857
AluBI AGCT 7 cut(s) 40, 172, 315, 605, 649, 788, 884
AluI AGCT 7 cut(s) 40, 172, 315, 605, 649, 788, 884
Alw21I GWGCWC 1 cut(s) 385
AlwI GGATC 1 cut(s) 257
AoxI GGCC 5 cut(s) 58, 93, 406, 570, 708
ApeKI GCWGC 7 cut(s) 136, 249, 344, 545, 632, 785, 788
Asp700I GAANNNNTTC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 406
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 503, 668
AsuNHI GCTAGC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 572
BanI GGYRCC 1 cut(s) 389
BauI CACGAG 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 385
BbvI GCAGC 7 cut(s) 148, 261, 356, 532, 619, 775, 797
BccI CCATC 6 cut(s) 90, 122, 187, 293, 664, 919
BciT130I CCWGG 2 cut(s) 97, 621
BcnI CCSGG 1 cut(s) 147
BfaI CTAG 4 cut(s) 173, 216, 291, 600
BfuAI ACCTGC 1 cut(s) 934
BisI GCNGC 7 cut(s) 137, 250, 345, 546, 633, 786, 789
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 7 cut(s) 138, 251, 346, 547, 634, 787, 790
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 3 cut(s) 97, 147, 621
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 2 cut(s) 283, 391
BmrFI CCNGG 3 cut(s) 97, 147, 621
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 6 cut(s) 2, 322, 430, 484, 606, 892
BmtI GCTAGC 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 359
BoxI GACNNNNGTC 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 533
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 2 cut(s) 422, 542
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 6 cut(s) 234, 253, 409, 474, 620, 705
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 936
Bse1I ACTGG 4 cut(s) 61, 354, 516, 817
BseBI CCWGG 2 cut(s) 97, 621
BseDI CCNNGG 6 cut(s) 234, 253, 409, 474, 620, 705
BseGI GGATG 1 cut(s) 675
BseLI CCNNNNNNNGG 2 cut(s) 146, 936
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 4 cut(s) 61, 354, 516, 817
BseXI GCAGC 7 cut(s) 148, 261, 356, 532, 619, 775, 797
BseYI CCCAGC 1 cut(s) 649
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 5 cut(s) 60, 95, 408, 572, 710
BshNI GGYRCC 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 385
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 2 cut(s) 146, 936
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 5 cut(s) 60, 95, 408, 572, 710
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 2 cut(s) 106, 262
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 2 cut(s) 74, 294
BspANI GGCC 5 cut(s) 60, 95, 408, 572, 710
BspCNI CTCAG 1 cut(s) 41
BspFNI CGCG 1 cut(s) 74
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 2 cut(s) 283, 391
BspMI ACCTGC 1 cut(s) 934
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 389
BsrI ACTGG 4 cut(s) 61, 354, 516, 817
BssECI CCNNGG 6 cut(s) 234, 253, 409, 474, 620, 705
BssMI GATC 2 cut(s) 106, 262
BssSI CACGAG 1 cut(s) 384
BssT1I CCWWGG 5 cut(s) 234, 253, 409, 474, 705
Bst2BI CACGAG 1 cut(s) 384
Bst2UI CCWGG 2 cut(s) 97, 621
Bst4CI ACNGT 1 cut(s) 592
Bst6I CTCTTC 2 cut(s) 639, 762
BstC8I GCNNGC 4 cut(s) 174, 317, 677, 882
BstDEI CTNAG 3 cut(s) 28, 549, 712
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 1 cut(s) 675
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 2 cut(s) 109, 265
BstMBI GATC 2 cut(s) 106, 262
BstMWI GCNNNNNNNGC 5 cut(s) 341, 389, 616, 785, 860
BstNI CCWGG 2 cut(s) 97, 621
BstPAI GACNNNNGTC 1 cut(s) 223
BstSCI CCNGG 3 cut(s) 95, 145, 619
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 7 cut(s) 148, 261, 356, 532, 619, 775, 797
BstXI CCANNNNNNTGG 1 cut(s) 511
BsuRI GGCC 5 cut(s) 60, 95, 408, 572, 710
BtgI CCRYGG 1 cut(s) 705
BtsCI GGATG 1 cut(s) 675
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 4 cut(s) 347, 465, 750, 810
BveI ACCTGC 1 cut(s) 934
Cac8I GCNNGC 4 cut(s) 174, 317, 677, 882
CciI TCATGA 1 cut(s) 763
Cfr13I GGNCC 1 cut(s) 406
CseI GACGC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 119
CviAII CATG 6 cut(s) 461, 505, 706, 764, 824, 936
CviQI GTAC 1 cut(s) 119
DdeI CTNAG 3 cut(s) 28, 549, 712
DpnI GATC 2 cut(s) 108, 264
DpnII GATC 2 cut(s) 106, 262
EaeI YGGCCR 1 cut(s) 570
Eam1104I CTCTTC 2 cut(s) 639, 762
EarI CTCTTC 2 cut(s) 639, 762
Eco130I CCWWGG 5 cut(s) 234, 253, 409, 474, 705
Eco147I AGGCCT 1 cut(s) 95
Eco57I CTGAAG 2 cut(s) 471, 489
EcoO109I RGGNCCY 1 cut(s) 406
EcoRII CCWGG 2 cut(s) 95, 619
EcoT14I CCWWGG 5 cut(s) 234, 253, 409, 474, 705
ErhI CCWWGG 5 cut(s) 234, 253, 409, 474, 705
FaeI CATG 6 cut(s) 464, 508, 709, 767, 827, 939
FaqI GGGAC 1 cut(s) 637
FatI CATG 6 cut(s) 460, 504, 705, 763, 823, 935
FauNDI CATATG 1 cut(s) 369
FblI GTMKAC 2 cut(s) 227, 723
Fnu4HI GCNGC 7 cut(s) 137, 250, 345, 546, 633, 786, 789
FokI GGATG 1 cut(s) 682
Fsp4HI GCNGC 7 cut(s) 137, 250, 345, 546, 633, 786, 789
FspBI CTAG 4 cut(s) 173, 216, 291, 600
GluI GCNGC 7 cut(s) 137, 250, 345, 546, 633, 786, 789
GsaI CCCAGC 1 cut(s) 653
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 5 cut(s) 60, 95, 408, 572, 710
HapII CCGG 1 cut(s) 147
HgaI GACGC 1 cut(s) 228
Hin1II CATG 6 cut(s) 464, 508, 709, 767, 827, 939
HinfI GANTC 5 cut(s) 481, 625, 734, 752, 819
HpaII CCGG 1 cut(s) 147
HphI GGTGA 2 cut(s) 503, 668
Hpy166II GTNNAC 2 cut(s) 228, 724
Hpy188I TCNGA 3 cut(s) 31, 48, 312
Hpy188III TCNNGA 6 cut(s) 125, 216, 401, 437, 578, 764
Hpy8I GTNNAC 2 cut(s) 228, 724
HpyAV CCTTC 4 cut(s) 71, 276, 495, 882
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4IV ACGT 1 cut(s) 858
HpyF10VI GCNNNNNNNGC 5 cut(s) 341, 389, 616, 785, 860
HpyF3I CTNAG 3 cut(s) 28, 549, 712
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 6 cut(s) 464, 508, 709, 767, 827, 939
Kzo9I GATC 2 cut(s) 106, 262
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 3 cut(s) 20, 149, 281
Lsp1109I GCAGC 7 cut(s) 148, 261, 356, 532, 619, 775, 797
LweI GCATC 6 cut(s) 2, 322, 430, 484, 606, 892
MaeI CTAG 4 cut(s) 173, 216, 291, 600
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 2 cut(s) 586, 921
MalI GATC 2 cut(s) 108, 264
MboI GATC 2 cut(s) 106, 262
MboII GAAGA 3 cut(s) 178, 656, 749
MhlI GDGCHC 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 5 cut(s) 153, 208, 242, 299, 498
MluNI TGGCCA 1 cut(s) 572
MlyI GAGTC 1 cut(s) 619
MnlI CCTC 4 cut(s) 37, 397, 508, 589
Mox20I TGGCCA 1 cut(s) 572
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 1 cut(s) 572
MseI TTAA 2 cut(s) 302, 806
MslI CAYNNNNRTG 2 cut(s) 368, 560
Msp20I TGGCCA 1 cut(s) 572
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 3 cut(s) 97, 147, 621
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 2 cut(s) 97, 621
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 5 cut(s) 341, 389, 616, 785, 860
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 705
NdeI CATATG 1 cut(s) 369
NdeII GATC 2 cut(s) 106, 262
NheI GCTAGC 1 cut(s) 172
NlaIII CATG 6 cut(s) 464, 508, 709, 767, 827, 939
NlaIV GGNNCC 2 cut(s) 283, 391
NmuCI GTSAC 1 cut(s) 586
PagI TCATGA 1 cut(s) 763
PceI AGGCCT 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 639
PctI GAATGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 4 cut(s) 481, 734, 752, 819
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 7 cut(s) 138, 251, 346, 547, 634, 787, 790
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PshAI GACNNNNGTC 1 cut(s) 223
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 95, 619
PspFI CCCAGC 1 cut(s) 649
PspGI CCWGG 2 cut(s) 95, 619
PspN4I GGNNCC 2 cut(s) 283, 391
PspPI GGNCC 1 cut(s) 406
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
RseI CAYNNNNRTG 2 cut(s) 368, 560
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 2 cut(s) 302, 806
SatI GCNGC 7 cut(s) 137, 250, 345, 546, 633, 786, 789
Sau3AI GATC 2 cut(s) 106, 262
Sau96I GGNCC 1 cut(s) 406
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 3 cut(s) 97, 147, 621
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 6 cut(s) 2, 322, 430, 484, 606, 892
SmiMI CAYNNNNRTG 2 cut(s) 368, 560
SmlI CTYRAG 2 cut(s) 401, 521
SmoI CTYRAG 2 cut(s) 401, 521
Sse9I AATT 5 cut(s) 153, 208, 242, 299, 498
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 2 cut(s) 74, 294
SspMI CTAG 4 cut(s) 173, 216, 291, 600
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 3 cut(s) 95, 145, 619
StyI CCWWGG 5 cut(s) 234, 253, 409, 474, 705
TaaI ACNGT 1 cut(s) 592
TaiI ACGT 1 cut(s) 861
TasI AATT 5 cut(s) 153, 208, 242, 299, 498
TatI WGTACW 1 cut(s) 118
TfiI GAWTC 4 cut(s) 481, 734, 752, 819
Tru1I TTAA 2 cut(s) 302, 806
Tru9I TTAA 2 cut(s) 302, 806
TscAI CASTG 4 cut(s) 354, 472, 750, 817
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 7 cut(s) 136, 249, 344, 545, 632, 785, 788
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 5 cut(s) 201, 356, 521, 657, 749
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 4 cut(s) 354, 472, 750, 817
XbaI TCTAGA 1 cut(s) 215
XmiI GTMKAC 2 cut(s) 227, 723
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 4 cut(s) 173, 216, 291, 600
ZrmI AGTACT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.