Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0007953

Physical Location & Seq

Genomic Coordinates Reverse (-)

55209745 .. 55218151

Gene Structure 8407 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0457700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGGCGATTGCCGTGTTGAATGCTTTGGATACTGCTCGAACACAATGGTACCACATGACGGCCATTGTCATTGCCGGAATGGGCTTCTTCACCGACGCCTATGACCTCTTCTGCATATCCACCGTTTCAAAACTCCTCGGCCGACTGTACTATTATGACCCCAGTACCGGAAAGCCCGGAAAGCTCCCCGTAAGCGTCAACAACTTAGTGACCGGAGTTGCCCTAGTCGGAACCCTATCGGGCCAACTATTCTTTGGCTACCTTGGAGACAAGCTTGGCCGCAAGAAAGTGTACGGCATCACCCTCATCATGATGGTCTTCTGCGCCATTTGCTCCGGCCTATCCTTCGGGTCTACAGCGGGTTCCGTGATGACAACCCTTTGTTTCTTTAGGTTTTGGCTTGGTTTTGGCATCGGAGGAGATTATCCCCTCTCCGCCACCATCATGTCGGAATATGCCAACAAATCAACACGTGGTGCTTTTATAGCGGCCGTGTTTGCCATGCAAGGAGTAGGGATTATCTTTGCGGGTTTGGTTTCCATGACACTCTCAGCACTCTTCCTCCACTACTACGACGCTCCTGCATTCAAAACAAAACCCCCTGTAGAGTATTCTGACGTGTTCTCCACGCAGCCGGAGGCCGACTACATGTGGAGGATTGTCCTCATGATCGGGGCTTTGCCGGCTATTGTCACGTTTTACTGGCGGATGAAAATGCCGGAAACGGCGCGTTACACGGCGTTGATCGAAGGCAACGCCAAGCAAGCAGCGACTGACATGGGTCAGGTCCTGGAGATTGAAATCCAAGCCGAACAGGACAAGGTGGCGCAGTTCAAAGCGGCAAACGAGTACACTTTGCTCTCCGGAGAGTTCTTCAGGCGGCACGGGAAACATCTCATCGGAACGATGACGACTTGGTTCTTGTTGGACATTGCGTTTTACAGCCAAAACTTGACTCAAAAGGACATTTTCCCGGCGATGGGTTTGACTGACCCAGCTAAAAAGGTCAATGCTCTTATGGAAGTCTTCCAAACCTCTAGGGCCATGTTTGTGGTTGCGTTACTAGGGACATTCCCGGGTTACTGGTTCACAGTGGCTTTCATCGAAAAGCTCGGACGATTCAAGATCCAACTCGTCGGGTTCTTCATGATGTCTGTGTTCATGCTCATCATTGGGTTCAAATACGCGTGGCTAAAAGATCACCCAGCAATATTTGCAACTCTCTATGGTCTGACATTTTTCTTCGCCAACTTTGGTCCGAACAGCACAACTTTCGTACTTCCGGCCGAGCTGTTTCCGACGAGGGTGAGGTCCACGTGCCATGCGATAAGTGCAGCGTCCGGGAAGGCTGGGGCGTTGATCGGTGCATTTGGTATACAAGACATAACTTTGGATGGAAAAGCCAAGTCGATTCAAAAGGCTATGATGATTTTGGCGTTTACCAACATGTTAGGGTTTTGTTTCACATTCATGGTCACCGAGACCAAAGGGCGGTCCTTGGAGGAGATCTCCGGCGAAGATGGTGACAACGGCGGCGAAAATGGAATACATATGGATGCACCGGTGTCTAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

524

Amino Acids

57.61

Weight (kDa)

8.53

Isoelectric Point (pI)

28.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	23 - 504	4.1e-52	Sugar (and other) transporter
MFS_1	PF07690	62 - 456	1.4e-22	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007953)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10460
fragaria_vesca	FvH4_1g00490 FvH4_1g00490 FvH4_1g00490 FvH4_1g00490 FvH4_1g00490 FvH4_1g00490
malus_domestica	MD15G1148400.v1.1
prunus_persica	Prupe.7G267200_v2.0.a1 Prupe.7G267200_v2.0.a1 Prupe.7G267200_v2.0.a1
pyrus_communis	pycom15g13270
rosa_chinensis	RchiOBHm_Chr2g0085091
rosa_laevigata	RLG00000015655
rosa_multiflora	Rmu_sc0008647.1_g000015 Rmu_sc0008647.1_g000017
rosa_roxburghii	Rroxscaffold_2G00155640
rosa_rugosa	Rorug01G0457600 Rorug01G0457700
rosa_samantha	Rh2AG005900 Rh2BG006600 Rh2CG007300 Rh2DG007400
rosa_wichuraiana	Rw2G000540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	48
AccB1I	GGYRCC	1 cut(s)	48
AccI	GTMKAC	2 cut(s)	353, 1375
AccII	CGCG	2 cut(s)	730, 1187
AccIII	TCCGGA	1 cut(s)	863
AclWI	GGATC	1 cut(s)	1120
AcoI	YGGCCR	5 cut(s)	60, 139, 277, 489, 1284
AcuI	CTGAAG	1 cut(s)	859
AcvI	CACGTG	2 cut(s)	473, 1317
AcyI	GRCGYC	1 cut(s)	96
AdeI	CACNNNGTG	1 cut(s)	476
AfaI	GTAC	6 cut(s)	50, 149, 166, 293, 851, 1278
AfiI	CCNNNNNNNGG	5 cut(s)	58, 167, 979, 980, 1491
AflIII	ACRYGT	5 cut(s)	470, 618, 648, 1185, 1446
AgeI	ACCGGT	1 cut(s)	1561
AgsI	TTSAA	8 cut(s)	19, 129, 589, 800, 835, 1123, 1180, 1415
AjiI	CACGTC	1 cut(s)	619
AjnI	CCWGG	1 cut(s)	789
AluBI	AGCT	5 cut(s)	184, 274, 998, 1111, 1291
AluI	AGCT	5 cut(s)	184, 274, 998, 1111, 1291
Alw26I	GTCTC	2 cut(s)	261, 1475
AlwI	GGATC	1 cut(s)	1120
AlwNI	CAGNNNCTG	2 cut(s)	773, 790
Ama87I	CYCGRG	1 cut(s)	1075
Aor13HI	TCCGGA	1 cut(s)	863
AoxI	GGCC	9 cut(s)	60, 139, 241, 277, 337, 489, 639, 1041, 1284
ApeKI	GCWGC	3 cut(s)	631, 767, 1334
AsiGI	ACCGGT	1 cut(s)	1561
Asp718I	GGTACC	1 cut(s)	48
AspLEI	GCGC	3 cut(s)	326, 730, 829
AspS9I	GGNCC	6 cut(s)	241, 787, 1041, 1256, 1311, 1494
AsuC2I	CCSGG	5 cut(s)	177, 974, 1076, 1077, 1342
AsuHPI	GGTGA	6 cut(s)	82, 292, 1193, 1318, 1468, 1535
AvaI	CYCGRG	1 cut(s)	1075
AvaII	GGWCC	4 cut(s)	787, 1256, 1311, 1494
BaeI	ACNNNNGTAYC	4 cut(s)	32, 65, 148, 181
BanI	GGYRCC	1 cut(s)	48
BbrPI	CACGTG	2 cut(s)	473, 1317
BbsI	GAAGAC	2 cut(s)	310, 1018
BbvI	GCAGC	3 cut(s)	643, 779, 1346
BccI	CCATC	5 cut(s)	307, 449, 973, 1388, 1514
BceAI	ACGGC	6 cut(s)	75, 310, 476, 741, 753, 1546
BcgI	CGANNNNNNTGC	4 cut(s)	563, 597, 1255, 1289
BciT130I	CCWGG	1 cut(s)	791
BciVI	GTATCC	1 cut(s)	22
BcnI	CCSGG	5 cut(s)	177, 974, 1076, 1077, 1342
BcoDI	GTCTC	2 cut(s)	261, 1475
BfaI	CTAG	3 cut(s)	224, 1038, 1064
BfmI	CTRYAG	2 cut(s)	354, 603
BfuI	GTATCC	1 cut(s)	22
BglII	AGATCT	1 cut(s)	1506
BisI	GCNGC	8 cut(s)	280, 489, 632, 768, 840, 881, 1335, 1534
BlsI	GCNGC	8 cut(s)	281, 490, 633, 769, 841, 882, 1336, 1535
Bme1390I	CCNGG	6 cut(s)	177, 791, 974, 1076, 1077, 1342
Bme18I	GGWCC	4 cut(s)	787, 1256, 1311, 1494
BmeT110I	CYCGRG	1 cut(s)	1075
BmgBI	CACGTC	1 cut(s)	619
BmgT120I	GGNCC	6 cut(s)	241, 787, 1041, 1256, 1311, 1494
BmiI	GGNNCC	3 cut(s)	50, 232, 364
BmrFI	CCNGG	6 cut(s)	177, 791, 974, 1076, 1077, 1342
BmrI	ACTGGG	1 cut(s)	156
BmsI	GCATC	3 cut(s)	306, 420, 1546
BmuI	ACTGGG	1 cut(s)	156
BoxI	GACNNNNGTC	1 cut(s)	780
BpiI	GAAGAC	2 cut(s)	310, 1018
BplI	GAGNNNNNCTC	2 cut(s)	1493, 1525
BpmI	CTGGAG	1 cut(s)	812
BpuMI	CCSGG	5 cut(s)	177, 974, 1076, 1077, 1342
BsaAI	YACGTR	2 cut(s)	473, 1317
BsaBI	GATNNNNATC	1 cut(s)	800
BsaHI	GRCGYC	1 cut(s)	96
BsaI	GGTCTC	1 cut(s)	1475
BsaJI	CCNNGG	4 cut(s)	136, 262, 1075, 1497
BsaWI	WCCGGW	4 cut(s)	167, 212, 863, 1561
BsaXI	ACNNNNNCTCC	4 cut(s)	546, 576, 608, 638
Bsc4I	CCNNNNNNNGG	5 cut(s)	58, 167, 979, 980, 1491
Bse118I	RCCGGY	2 cut(s)	682, 1561
Bse1I	ACTGG	3 cut(s)	162, 707, 1088
Bse3DI	GCAATG	2 cut(s)	69, 930
Bse8I	GATNNNNATC	1 cut(s)	800
BseAI	TCCGGA	1 cut(s)	863
BseBI	CCWGG	1 cut(s)	791
BseDI	CCNNGG	4 cut(s)	136, 262, 1075, 1497
BseGI	GGATG	3 cut(s)	714, 1399, 1561
BseJI	GATNNNNATC	1 cut(s)	800
BseLI	CCNNNNNNNGG	5 cut(s)	58, 167, 979, 980, 1491
BseMI	GCAATG	2 cut(s)	69, 930
BseMII	CTCAG	1 cut(s)	564
BseNI	ACTGG	3 cut(s)	162, 707, 1088
BseRI	GAGGAG	3 cut(s)	125, 432, 1517
BseX3I	CGGCCG	3 cut(s)	139, 489, 1284
BseXI	GCAGC	3 cut(s)	643, 779, 1346
BseYI	CCCAGC	3 cut(s)	994, 1204, 1349
BsgI	GTGCAG	1 cut(s)	1353
Bsh1236I	CGCG	2 cut(s)	730, 1187
Bsh1285I	CGRYCG	3 cut(s)	142, 492, 1287
BshFI	GGCC	9 cut(s)	62, 141, 243, 279, 339, 491, 641, 1043, 1286
BshNI	GGYRCC	1 cut(s)	48
BshTI	ACCGGT	1 cut(s)	1561
BsiEI	CGRYCG	3 cut(s)	142, 492, 1287
BsiHKCI	CYCGRG	1 cut(s)	1075
BslFI	GGGAC	1 cut(s)	1081
BslI	CCNNNNNNNGG	5 cut(s)	58, 167, 979, 980, 1491
BsmAI	GTCTC	2 cut(s)	261, 1475
BsmFI	GGGAC	1 cut(s)	1081
BsmI	GAATGC	2 cut(s)	25, 584
BsnI	GGCC	9 cut(s)	62, 141, 243, 279, 339, 491, 641, 1043, 1286
Bso31I	GGTCTC	1 cut(s)	1475
BsoBI	CYCGRG	1 cut(s)	1075
Bsp13I	TCCGGA	1 cut(s)	863
Bsp143I	GATC	6 cut(s)	669, 744, 1125, 1198, 1359, 1506
BspANI	GGCC	9 cut(s)	62, 141, 243, 279, 339, 491, 641, 1043, 1286
BspCNI	CTCAG	1 cut(s)	563
BspEI	TCCGGA	1 cut(s)	863
BspFNI	CGCG	2 cut(s)	730, 1187
BspHI	TCATGA	3 cut(s)	309, 666, 1146
BspLI	GGNNCC	3 cut(s)	50, 232, 364
BspPI	GGATC	1 cut(s)	1120
BspT107I	GGYRCC	1 cut(s)	48
BspTNI	GGTCTC	1 cut(s)	1475
BsrDI	GCAATG	2 cut(s)	69, 930
BsrFI	RCCGGY	2 cut(s)	682, 1561
BsrI	ACTGG	3 cut(s)	162, 707, 1088
BssAI	RCCGGY	2 cut(s)	682, 1561
BssECI	CCNNGG	4 cut(s)	136, 262, 1075, 1497
BssMI	GATC	6 cut(s)	669, 744, 1125, 1198, 1359, 1506
BssNAI	GTATAC	1 cut(s)	1376
BssNI	GRCGYC	1 cut(s)	96
BssT1I	CCWWGG	2 cut(s)	262, 1497
Bst1107I	GTATAC	1 cut(s)	1376
Bst2UI	CCWGG	1 cut(s)	791
Bst4CI	ACNGT	3 cut(s)	124, 147, 1093
Bst6I	CTCTTC	2 cut(s)	113, 563
BstACI	GRCGYC	1 cut(s)	96
BstAPI	GCANNNNNTGC	1 cut(s)	1214
BstBAI	YACGTR	2 cut(s)	473, 1317
BstC8I	GCNNGC	2 cut(s)	684, 765
BstDEI	CTNAG	3 cut(s)	205, 550, 1569
BstEII	GGTNACC	1 cut(s)	1474
BstF5I	GGATG	3 cut(s)	714, 1399, 1561
BstFNI	CGCG	2 cut(s)	730, 1187
BstHHI	GCGC	3 cut(s)	326, 730, 829
BstKTI	GATC	6 cut(s)	672, 747, 1128, 1201, 1362, 1509
BstMAI	GTCTC	2 cut(s)	261, 1475
BstMBI	GATC	6 cut(s)	669, 744, 1125, 1198, 1359, 1506
BstMCI	CGRYCG	3 cut(s)	142, 492, 1287
BstMWI	GCNNNNNNNGC	8 cut(s)	181, 330, 485, 497, 683, 764, 1214, 1331
BstNI	CCWGG	1 cut(s)	791
BstNSI	RCATGY	2 cut(s)	652, 1450
BstPAI	GACNNNNGTC	1 cut(s)	780
BstPI	GGTNACC	1 cut(s)	1474
BstSCI	CCNGG	6 cut(s)	175, 789, 972, 1074, 1075, 1340
BstSFI	CTRYAG	2 cut(s)	354, 603
BstUI	CGCG	2 cut(s)	730, 1187
BstV1I	GCAGC	3 cut(s)	643, 779, 1346
BstV2I	GAAGAC	2 cut(s)	310, 1018
BstX2I	RGATCY	2 cut(s)	1125, 1506
BstXI	CCANNNNNNTGG	1 cut(s)	1051
BstYI	RGATCY	2 cut(s)	1125, 1506
BstZ17I	GTATAC	1 cut(s)	1376
BstZI	CGGCCG	3 cut(s)	139, 489, 1284
BsuI	GTATCC	1 cut(s)	22
BsuRI	GGCC	9 cut(s)	62, 141, 243, 279, 339, 491, 641, 1043, 1286
BtgZI	GCGATG	1 cut(s)	992
BtrI	CACGTC	1 cut(s)	619
BtsCI	GGATG	3 cut(s)	714, 1399, 1561
BtsIMutI	CAGTG	1 cut(s)	1098
Cac8I	GCNNGC	2 cut(s)	684, 765
CaiI	CAGNNNCTG	2 cut(s)	773, 790
CciI	TCATGA	3 cut(s)	309, 666, 1146
CfoI	GCGC	3 cut(s)	326, 730, 829
Cfr10I	RCCGGY	2 cut(s)	682, 1561
Cfr13I	GGNCC	6 cut(s)	241, 787, 1041, 1256, 1311, 1494
Cfr9I	CCCGGG	1 cut(s)	1075
CseI	GACGC	4 cut(s)	104, 184, 584, 1326
Csp6I	GTAC	6 cut(s)	49, 148, 165, 292, 850, 1277
CspAI	ACCGGT	1 cut(s)	1561
CviQI	GTAC	6 cut(s)	49, 148, 165, 292, 850, 1277
DdeI	CTNAG	3 cut(s)	205, 550, 1569
DpnI	GATC	6 cut(s)	671, 746, 1127, 1200, 1361, 1508
DpnII	GATC	6 cut(s)	669, 744, 1125, 1198, 1359, 1506
DraIII	CACNNNGTG	1 cut(s)	476
EaeI	YGGCCR	5 cut(s)	60, 139, 277, 489, 1284
EagI	CGGCCG	3 cut(s)	139, 489, 1284
Eam1104I	CTCTTC	2 cut(s)	113, 563
EarI	CTCTTC	2 cut(s)	113, 563
EciI	GGCGGA	2 cut(s)	424, 721
EclXI	CGGCCG	3 cut(s)	139, 489, 1284
Eco130I	CCWWGG	2 cut(s)	262, 1497
Eco31I	GGTCTC	1 cut(s)	1475
Eco47I	GGWCC	4 cut(s)	787, 1256, 1311, 1494
Eco52I	CGGCCG	3 cut(s)	139, 489, 1284
Eco57I	CTGAAG	1 cut(s)	859
Eco72I	CACGTG	2 cut(s)	473, 1317
Eco88I	CYCGRG	1 cut(s)	1075
Eco91I	GGTNACC	1 cut(s)	1474
EcoO109I	RGGNCCY	1 cut(s)	787
EcoO65I	GGTNACC	1 cut(s)	1474
EcoRII	CCWGG	1 cut(s)	789
EcoT14I	CCWWGG	2 cut(s)	262, 1497
ErhI	CCWWGG	2 cut(s)	262, 1497
FaqI	GGGAC	1 cut(s)	1081
FauI	CCCGC	2 cut(s)	352, 520
FauNDI	CATATG	1 cut(s)	1551
FblI	GTMKAC	2 cut(s)	353, 1375
Fnu4HI	GCNGC	8 cut(s)	280, 489, 632, 768, 840, 881, 1335, 1534
FokI	GGATG	3 cut(s)	721, 1406, 1568
Fsp4HI	GCNGC	8 cut(s)	280, 489, 632, 768, 840, 881, 1335, 1534
FspBI	CTAG	3 cut(s)	224, 1038, 1064
GlaI	GCGC	3 cut(s)	325, 729, 828
GluI	GCNGC	8 cut(s)	280, 489, 632, 768, 840, 881, 1335, 1534
GsaI	CCCAGC	3 cut(s)	998, 1208, 1353
GsuI	CTGGAG	1 cut(s)	812
HaeIII	GGCC	9 cut(s)	62, 141, 243, 279, 339, 491, 641, 1043, 1286
HgaI	GACGC	4 cut(s)	104, 184, 584, 1326
HhaI	GCGC	3 cut(s)	326, 730, 829
Hin1I	GRCGYC	1 cut(s)	96
Hin6I	GCGC	3 cut(s)	324, 728, 827
HinP1I	GCGC	3 cut(s)	324, 728, 827
HincII	GTYRAC	1 cut(s)	199
HindII	GTYRAC	1 cut(s)	199
HindIII	AAGCTT	1 cut(s)	272
HinfI	GANTC	3 cut(s)	955, 1119, 1411
HphI	GGTGA	6 cut(s)	82, 292, 1193, 1318, 1468, 1535
Hpy166II	GTNNAC	8 cut(s)	199, 292, 354, 852, 1089, 1314, 1376, 1440
Hpy188I	TCNGA	9 cut(s)	230, 416, 451, 616, 902, 1115, 1233, 1260, 1299
Hpy188III	TCNNGA	5 cut(s)	310, 667, 864, 1123, 1147
Hpy8I	GTNNAC	8 cut(s)	199, 292, 354, 852, 1089, 1314, 1376, 1440
Hpy99I	CGWCG	4 cut(s)	98, 578, 1139, 1303
HpyAV	CCTTC	3 cut(s)	355, 743, 1339
HpyCH4III	ACNGT	3 cut(s)	124, 147, 1093
HpyCH4IV	ACGT	4 cut(s)	472, 618, 695, 1316
HpyCH4V	TGCA	7 cut(s)	114, 505, 584, 1217, 1334, 1367, 1559
HpyF10VI	GCNNNNNNNGC	8 cut(s)	181, 330, 485, 497, 683, 764, 1214, 1331
HpyF3I	CTNAG	3 cut(s)	205, 550, 1569
HpySE526I	ACGT	4 cut(s)	472, 618, 695, 1316
Hsp92I	GRCGYC	1 cut(s)	96
HspAI	GCGC	3 cut(s)	324, 728, 827
Kpn2I	TCCGGA	1 cut(s)	863
KpnI	GGTACC	1 cut(s)	52
KroI	GCCGGC	1 cut(s)	682
KroNI	GCCGGC	1 cut(s)	684
Kzo9I	GATC	6 cut(s)	669, 744, 1125, 1198, 1359, 1506
LmnI	GCTCC	3 cut(s)	189, 338, 583
Lsp1109I	GCAGC	3 cut(s)	643, 779, 1346
LweI	GCATC	3 cut(s)	306, 420, 1546
MaeI	CTAG	3 cut(s)	224, 1038, 1064
MaeII	ACGT	4 cut(s)	472, 618, 695, 1316
MaeIII	GTNAC	7 cut(s)	208, 691, 731, 1059, 1079, 1474, 1523
MalI	GATC	6 cut(s)	671, 746, 1127, 1200, 1361, 1508
MboI	GATC	6 cut(s)	669, 744, 1125, 1198, 1359, 1506
MboII	GAAGA	9 cut(s)	79, 100, 310, 550, 865, 1018, 1135, 1234, 1529
MflI	RGATCY	2 cut(s)	1125, 1506
MluI	ACGCGT	1 cut(s)	1185
MlyI	GAGTC	1 cut(s)	949
MmeI	TCCRAC	5 cut(s)	208, 429, 906, 1153, 1322
MroI	TCCGGA	1 cut(s)	863
MroNI	GCCGGC	1 cut(s)	682
MslI	CAYNNNNRTG	5 cut(s)	311, 443, 1049, 1469, 1554
MspA1I	CMGCKG	1 cut(s)	359
MspR9I	CCNGG	6 cut(s)	177, 791, 974, 1076, 1077, 1342
Mva1269I	GAATGC	2 cut(s)	25, 584
MvaI	CCWGG	1 cut(s)	791
MvnI	CGCG	2 cut(s)	730, 1187
MwoI	GCNNNNNNNGC	8 cut(s)	181, 330, 485, 497, 683, 764, 1214, 1331
NaeI	GCCGGC	1 cut(s)	684
NciI	CCSGG	5 cut(s)	177, 974, 1076, 1077, 1342
NdeI	CATATG	1 cut(s)	1551
NdeII	GATC	6 cut(s)	669, 744, 1125, 1198, 1359, 1506
NgoMIV	GCCGGC	1 cut(s)	682
NlaIV	GGNNCC	3 cut(s)	50, 232, 364
NmeAIII	GCCGAG	2 cut(s)	117, 1312
NmuCI	GTSAC	4 cut(s)	208, 691, 1474, 1523
NspI	RCATGY	2 cut(s)	652, 1450
PagI	TCATGA	3 cut(s)	309, 666, 1146
PciI	ACATGT	2 cut(s)	648, 1446
PcsI	WCGNNNNNNNCGW	1 cut(s)	1322
PctI	GAATGC	2 cut(s)	25, 584
PdiI	GCCGGC	1 cut(s)	684
PfeI	GAWTC	2 cut(s)	1119, 1411
PfoI	TCCNGGA	2 cut(s)	789, 1340
PinAI	ACCGGT	1 cut(s)	1561
PkrI	GCNGC	8 cut(s)	281, 490, 633, 769, 841, 882, 1336, 1535
PleI	GAGTC	1 cut(s)	949
PmaCI	CACGTG	2 cut(s)	473, 1317
PmlI	CACGTG	2 cut(s)	473, 1317
PpsI	GAGTC	1 cut(s)	949
Ppu21I	YACGTR	2 cut(s)	473, 1317
PpuMI	RGGWCCY	1 cut(s)	787
PscI	ACATGT	2 cut(s)	648, 1446
PshAI	GACNNNNGTC	1 cut(s)	780
Psp5II	RGGWCCY	1 cut(s)	787
Psp6I	CCWGG	1 cut(s)	789
PspCI	CACGTG	2 cut(s)	473, 1317
PspEI	GGTNACC	1 cut(s)	1474
PspFI	CCCAGC	3 cut(s)	994, 1204, 1349
PspGI	CCWGG	1 cut(s)	789
PspN4I	GGNNCC	3 cut(s)	50, 232, 364
PspPI	GGNCC	6 cut(s)	241, 787, 1041, 1256, 1311, 1494
PspPPI	RGGWCCY	1 cut(s)	787
PstNI	CAGNNNCTG	2 cut(s)	773, 790
PsuI	RGATCY	2 cut(s)	1125, 1506
RsaI	GTAC	6 cut(s)	50, 149, 166, 293, 851, 1278
RsaNI	GTAC	6 cut(s)	49, 148, 165, 292, 850, 1277
RseI	CAYNNNNRTG	5 cut(s)	311, 443, 1049, 1469, 1554
SatI	GCNGC	8 cut(s)	280, 489, 632, 768, 840, 881, 1335, 1534
Sau3AI	GATC	6 cut(s)	669, 744, 1125, 1198, 1359, 1506
Sau96I	GGNCC	6 cut(s)	241, 787, 1041, 1256, 1311, 1494
SchI	GAGTC	1 cut(s)	949
ScrFI	CCNGG	6 cut(s)	177, 791, 974, 1076, 1077, 1342
SfaNI	GCATC	3 cut(s)	306, 420, 1546
SfcI	CTRYAG	2 cut(s)	354, 603
SgrAI	CRCCGGYG	1 cut(s)	1561
SinI	GGWCC	4 cut(s)	787, 1256, 1311, 1494
SmaI	CCCGGG	1 cut(s)	1077
SmiMI	CAYNNNNRTG	5 cut(s)	311, 443, 1049, 1469, 1554
SspI	AATATT	1 cut(s)	1212
SspMI	CTAG	3 cut(s)	224, 1038, 1064
StyD4I	CCNGG	6 cut(s)	175, 789, 972, 1074, 1075, 1340
StyI	CCWWGG	2 cut(s)	262, 1497
TaaI	ACNGT	3 cut(s)	124, 147, 1093
TaiI	ACGT	4 cut(s)	475, 621, 698, 1319
TaqI	TCGA	4 cut(s)	37, 747, 1104, 1409
TatI	WGTACW	2 cut(s)	147, 849
TauI	GCSGC	5 cut(s)	282, 491, 842, 883, 1536
TfiI	GAWTC	2 cut(s)	1119, 1411
TscAI	CASTG	1 cut(s)	1098
TseFI	GTSAC	4 cut(s)	208, 691, 1474, 1523
TseI	GCWGC	3 cut(s)	631, 767, 1334
Tsp45I	GTSAC	4 cut(s)	208, 691, 1474, 1523
TspDTI	ATGAA	5 cut(s)	725, 1090, 1135, 1150, 1459
TspGWI	ACGGA	1 cut(s)	355
TspMI	CCCGGG	1 cut(s)	1075
TspRI	CASTG	1 cut(s)	1098
VpaK11BI	GGWCC	4 cut(s)	787, 1256, 1311, 1494
XceI	RCATGY	2 cut(s)	652, 1450
XcmI	CCANNNNNNNNNTGG	1 cut(s)	251
XmaI	CCCGGG	1 cut(s)	1075
XmiI	GTMKAC	2 cut(s)	353, 1375
XspI	CTAG	3 cut(s)	224, 1038, 1064

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.