Rh2BG006600

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007953
Physical Location & Seq
Reverse (-)
476402 .. 478900
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG006600.1

Sequence Viewer

Length: 645 bp
ATGATGCCCAGATGCTCCGTTCAATCCTCAGATGTTCAGCTCTTTCAGACCATAACTGGCCTTCAACAACTCGCCGAAACCCACCGTTTCAAGGCCTGGTTATTAGATCAGTTTGGAGTACTTCACGATGGGAAGCAGCCTTATCCCGGAGCAATTTCAACATTGAAGAAGCTAGCAAATAGTGGTGCAAAGATGGTTATTATAAGTAATTCGTCTAGACGCTCGTCTACGACATTGGAGAAATTGAGCAGCCTTGGGTTTGATCCTTCTCTCTTTGTGGGAGCCATCACTAGCGGGGAATTAACACATCAGAGCTTGCAAAGGAGAGATGATGCTTGGTTTGCAGCACTGGGAAAATCTTGCATTCATATGACTTGGAGTGCTCGTGGTGCCATATCTCTTGAGGGCCTTGGATTACAAGTTGTGGAAAATGTTCAAGATGCTGACTTTATTTTGGCTCATGGCACTGAAGCCTTGGGGAATCCTTCAGGTGATGCAACTCCCATGAAACTGGAGGAACTTGAGAATATATTGGAACAATGTGCTGCTAAGCACATTCCTATGGTGGTGGCCAATCCTGATTTTGTGACTGTTGAGGCTAGAGCTTTGCGTGTGATGCCGGGTAAAACTAGCTGTTTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

214

Amino Acids

23.2

Weight (kDa)

6.29

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 33 - 119 1.7e-15 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 227
AciI CCGC 1 cut(s) 294
AclWI GGATC 1 cut(s) 257
AcoI YGGCCR 1 cut(s) 570
AcuI CTGAAG 2 cut(s) 471, 489
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 2 cut(s) 91, 146
AgsI TTSAA 6 cut(s) 23, 65, 91, 159, 166, 437
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 5 cut(s) 40, 172, 315, 605, 633
AluI AGCT 5 cut(s) 40, 172, 315, 605, 633
Alw21I GWGCWC 1 cut(s) 385
AlwI GGATC 1 cut(s) 257
AoxI GGCC 4 cut(s) 58, 93, 406, 570
ApeKI GCWGC 4 cut(s) 136, 249, 344, 545
Asp700I GAANNNNTTC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 406
AsuC2I CCSGG 2 cut(s) 147, 621
AsuHPI GGTGA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 572
BanI GGYRCC 1 cut(s) 389
BauI CACGAG 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 385
BbvI GCAGC 4 cut(s) 148, 261, 356, 532
BccI CCATC 3 cut(s) 122, 187, 293
BciT130I CCWGG 1 cut(s) 97
BcnI CCSGG 2 cut(s) 147, 621
BfaI CTAG 5 cut(s) 173, 216, 291, 600, 630
BisI GCNGC 4 cut(s) 137, 250, 345, 546
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 4 cut(s) 138, 251, 346, 547
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 3 cut(s) 97, 147, 621
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 2 cut(s) 283, 391
BmrFI CCNGG 3 cut(s) 97, 147, 621
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 5 cut(s) 2, 322, 430, 484, 606
BmtI GCTAGC 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 359
BoxI GACNNNNGTC 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 533
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 2 cut(s) 422, 542
BpuMI CCSGG 2 cut(s) 147, 621
BsaJI CCNNGG 3 cut(s) 253, 409, 474
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 146
Bse1I ACTGG 3 cut(s) 61, 354, 516
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 3 cut(s) 253, 409, 474
BseLI CCNNNNNNNGG 2 cut(s) 91, 146
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 3 cut(s) 61, 354, 516
BseXI GCAGC 4 cut(s) 148, 261, 356, 532
BshFI GGCC 4 cut(s) 60, 95, 408, 572
BshNI GGYRCC 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 385
BsiSI CCGG 2 cut(s) 147, 620
BslI CCNNNNNNNGG 2 cut(s) 91, 146
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 4 cut(s) 60, 95, 408, 572
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 2 cut(s) 106, 262
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 1 cut(s) 294
BspANI GGCC 4 cut(s) 60, 95, 408, 572
BspCNI CTCAG 1 cut(s) 41
BspLI GGNNCC 2 cut(s) 283, 391
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 389
BsrI ACTGG 3 cut(s) 61, 354, 516
BssECI CCNNGG 3 cut(s) 253, 409, 474
BssMI GATC 2 cut(s) 106, 262
BssSI CACGAG 1 cut(s) 384
BssT1I CCWWGG 3 cut(s) 253, 409, 474
Bst2BI CACGAG 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 2 cut(s) 86, 592
BstC8I GCNNGC 2 cut(s) 174, 317
BstDEI CTNAG 2 cut(s) 28, 549
BstKTI GATC 2 cut(s) 109, 265
BstMBI GATC 2 cut(s) 106, 262
BstMWI GCNNNNNNNGC 4 cut(s) 341, 389, 616, 639
BstNI CCWGG 1 cut(s) 97
BstPAI GACNNNNGTC 1 cut(s) 223
BstSCI CCNGG 3 cut(s) 95, 145, 619
BstV1I GCAGC 4 cut(s) 148, 261, 356, 532
BstXI CCANNNNNNTGG 1 cut(s) 511
BsuRI GGCC 4 cut(s) 60, 95, 408, 572
BtsIMutI CAGTG 2 cut(s) 347, 465
Cac8I GCNNGC 2 cut(s) 174, 317
Cfr13I GGNCC 1 cut(s) 406
CseI GACGC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 119
CviAII CATG 2 cut(s) 461, 505
CviQI GTAC 1 cut(s) 119
DdeI CTNAG 2 cut(s) 28, 549
DpnI GATC 2 cut(s) 108, 264
DpnII GATC 2 cut(s) 106, 262
EaeI YGGCCR 1 cut(s) 570
Eco130I CCWWGG 3 cut(s) 253, 409, 474
Eco147I AGGCCT 1 cut(s) 95
Eco57I CTGAAG 2 cut(s) 471, 489
EcoO109I RGGNCCY 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 95
EcoT14I CCWWGG 3 cut(s) 253, 409, 474
ErhI CCWWGG 3 cut(s) 253, 409, 474
FaeI CATG 2 cut(s) 464, 508
FaiI YATR 9 cut(s) 53, 203, 369, 371, 395, 462, 506, 530, 563
FatI CATG 2 cut(s) 460, 504
FauI CCCGC 1 cut(s) 287
FauNDI CATATG 1 cut(s) 369
FblI GTMKAC 1 cut(s) 227
Fnu4HI GCNGC 4 cut(s) 137, 250, 345, 546
Fsp4HI GCNGC 4 cut(s) 137, 250, 345, 546
FspBI CTAG 5 cut(s) 173, 216, 291, 600, 630
GluI GCNGC 4 cut(s) 137, 250, 345, 546
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 4 cut(s) 60, 95, 408, 572
HapII CCGG 2 cut(s) 147, 620
HgaI GACGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 464, 508
HinfI GANTC 1 cut(s) 481
HpaII CCGG 2 cut(s) 147, 620
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 3 cut(s) 31, 48, 312
Hpy188III TCNNGA 5 cut(s) 125, 216, 401, 437, 578
Hpy8I GTNNAC 1 cut(s) 228
HpyAV CCTTC 3 cut(s) 71, 276, 495
HpyCH4III ACNGT 2 cut(s) 86, 592
HpyCH4V TGCA 5 cut(s) 188, 319, 344, 363, 497
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 389, 616, 639
HpyF3I CTNAG 2 cut(s) 28, 549
Hsp92II CATG 2 cut(s) 464, 508
Kzo9I GATC 2 cut(s) 106, 262
LmnI GCTCC 3 cut(s) 20, 149, 281
Lsp1109I GCAGC 4 cut(s) 148, 261, 356, 532
LweI GCATC 5 cut(s) 2, 322, 430, 484, 606
MaeI CTAG 5 cut(s) 173, 216, 291, 600, 630
MaeIII GTNAC 1 cut(s) 586
MalI GATC 2 cut(s) 108, 264
MboI GATC 2 cut(s) 106, 262
MboII GAAGA 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 4 cut(s) 153, 208, 242, 299
MluNI TGGCCA 1 cut(s) 572
MnlI CCTC 4 cut(s) 37, 397, 508, 589
Mox20I TGGCCA 1 cut(s) 572
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 1 cut(s) 572
MseI TTAA 1 cut(s) 302
MslI CAYNNNNRTG 2 cut(s) 368, 560
Msp20I TGGCCA 1 cut(s) 572
MspI CCGG 2 cut(s) 147, 620
MspR9I CCNGG 3 cut(s) 97, 147, 621
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 4 cut(s) 341, 389, 616, 639
NciI CCSGG 2 cut(s) 147, 621
NdeI CATATG 1 cut(s) 369
NdeII GATC 2 cut(s) 106, 262
NheI GCTAGC 1 cut(s) 172
NlaIII CATG 2 cut(s) 464, 508
NlaIV GGNNCC 2 cut(s) 283, 391
NmuCI GTSAC 1 cut(s) 586
PceI AGGCCT 1 cut(s) 95
PctI GAATGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 1 cut(s) 481
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 4 cut(s) 138, 251, 346, 547
PshAI GACNNNNGTC 1 cut(s) 223
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 283, 391
PspPI GGNCC 1 cut(s) 406
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
RseI CAYNNNNRTG 2 cut(s) 368, 560
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 4 cut(s) 137, 250, 345, 546
Sau3AI GATC 2 cut(s) 106, 262
Sau96I GGNCC 1 cut(s) 406
ScaI AGTACT 1 cut(s) 120
ScrFI CCNGG 3 cut(s) 97, 147, 621
SduI GDGCHC 1 cut(s) 385
SetI ASST 6 cut(s) 42, 174, 317, 493, 607, 635
SfaNI GCATC 5 cut(s) 2, 322, 430, 484, 606
SmiMI CAYNNNNRTG 2 cut(s) 368, 560
SmlI CTYRAG 2 cut(s) 401, 521
SmoI CTYRAG 2 cut(s) 401, 521
Sse9I AATT 4 cut(s) 153, 208, 242, 299
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 1 cut(s) 294
SspMI CTAG 5 cut(s) 173, 216, 291, 600, 630
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 3 cut(s) 95, 145, 619
StyI CCWWGG 3 cut(s) 253, 409, 474
TaaI ACNGT 2 cut(s) 86, 592
TasI AATT 4 cut(s) 153, 208, 242, 299
TatI WGTACW 1 cut(s) 118
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TscAI CASTG 2 cut(s) 354, 472
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 4 cut(s) 136, 249, 344, 545
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 2 cut(s) 356, 521
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 2 cut(s) 354, 472
XbaI TCTAGA 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 5 cut(s) 173, 216, 291, 600, 630
ZrmI AGTACT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.