AT5G10910

MraW methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010420
Physical Location & Seq
Forward (+)
3439553 .. 3441744
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10910.1

Sequence Viewer

Length: 1305 bp
ATGGCGGGAGTGATTAGGGCTAAGCAGCTGTTTCTATGCTCGTCGATTCTCTCGAGCAACAACAATAAGGTTCTATCACGCCTTCCTCGGCGTAGTATCAATGTAATTGCGGGAAATCTCAATTCGAGCGAGACTAAGAAGAAGGAGAAAGAGAAGAGGAAGAGGAGGAAAGAGATTGAGGTGGAAAAAGCTACTGCGGAAGCAGTGGTCAATAAGGAAAAGAGAAGGACACGGTCGAGCAGAGGGTATGAACTCGCCGACGGTGACGAAGTTCCGTCCTCACATGTGCCTGTGATGCTCGGAGAGGTTCTTGACATTTTCTCTTCCGTTCGCTTGCGAAGTTTCGTTGATTGTACTCTTGGCGCAGCTGGTCACTCTTCTTCTATCATTCAAAGCCACTCTGAATTGAAGAATTTTGTTGGAATGGATGTTGATCCTGTGGCAAGGAAACTGGGACACTTTCATATTGATTCGCTCATGCATCCAACTTTGAAGGCTTCTATAGTGTTGAAGAACTTCAAATACATCAAATCTGTGATTGCTGATACGCAACCGGAGTTGTTAGATGTAGGTGTTGATGGTATTCTTATGGACCTGGGAATGTCATCTATGCAGGTAAACAATCCTGAAAGAGGGTTTAGTGTTCTTCAAGAGGGACCTCTTGATATGCGTATGGATCCTCAGGCGACTCTAACAGCAGAAGATATAGTGAATTCGTGGCCAGAATCTGAACTGGGAAGAGTTCTACGGGATTATGGCGAAGAAAGCAATTGGTATTTACTTCAAAATAGAATTGTTAAGGCTCGACTTAATGGCGGGTTACATTCTACTGGTGAACTGGTGGATCTCATTCGGGGTACGTCACCAGCAAGCAGAGGAGGCAGACAAGGCTGGATAAAGACAGCAACACGTGTGTTTCAAGGCCTAAGGATTGCAGTGAACGACGAACTCAAGACACTACAAAACTCACTCTACTCATCATTCGATGTTCTCGCTCCAGGTGGGAGACTAGCAGTTATCTCCTTTCACAGTCTGGAGGATCGGGTTGTAAAACAAACGTTCCTCGACATCTTAGGGTTTCAAAGAGAAGAGATCAACGGAGAGGGAAGTAGTGTAAAACCGGAAAGGCAAATCGAAGAAAGAGTAGAGAAGGAATTAAAAGAGAAAGAAGAGTGGATCAAACAGACAGTAATAGCGTCGAAAGGAGTAATACTGACAAAGAGACCAATAACACCTTCAGAGGAAGAGGAAAGACTGAATCGCAGAGCAAGAAGTGCTAAGTTAAGAGTGATTCAGAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

434

Amino Acids

48.62

Weight (kDa)

9.35

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 94 - 433 1.7e-82 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 604
AciI CCGC 4 cut(s) 5, 110, 197, 816
AclI AACGTT 1 cut(s) 1058
AclWI GGATC 6 cut(s) 428, 671, 684, 852, 1047, 1184
AcoI YGGCCR 1 cut(s) 719
AcsI RAATTY 2 cut(s) 412, 712
AcuI CTGAAG 1 cut(s) 1221
AcvI CACGTG 1 cut(s) 911
AfaI GTAC 2 cut(s) 355, 859
AfiI CCNNNNNNNGG 1 cut(s) 632
AflIII ACRYGT 3 cut(s) 283, 908, 910
AgsI TTSAA 9 cut(s) 392, 409, 493, 511, 520, 650, 785, 920, 1082
AjnI CCWGG 2 cut(s) 594, 997
AjuI GAANNNNNNNTTGG 2 cut(s) 1219, 1251
AluBI AGCT 3 cut(s) 28, 191, 368
AluI AGCT 3 cut(s) 28, 191, 368
Alw26I GTCTC 3 cut(s) 125, 1000, 1216
AlwI GGATC 6 cut(s) 428, 671, 684, 852, 1047, 1184
AlwNI CAGNNNCTG 1 cut(s) 728
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 2 cut(s) 719, 922
ApeKI GCWGC 2 cut(s) 25, 365
ApoI RAATTY 2 cut(s) 412, 712
Asp700I GAANNNNTTC 1 cut(s) 515
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 2 cut(s) 592, 656
AsuHPI GGTGA 3 cut(s) 275, 845, 855
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 2 cut(s) 592, 656
AxyI CCTNAGG 2 cut(s) 681, 926
BalI TGGCCA 1 cut(s) 721
BamHI GGATCC 1 cut(s) 676
BbrPI CACGTG 1 cut(s) 911
BbvI GCAGC 2 cut(s) 37, 377
BccI CCATC 1 cut(s) 572
BciT130I CCWGG 2 cut(s) 596, 999
BcoDI GTCTC 3 cut(s) 125, 1000, 1216
BfaI CTAG 1 cut(s) 1010
BfmI CTRYAG 1 cut(s) 501
BfuAI ACCTGC 1 cut(s) 604
BisI GCNGC 2 cut(s) 26, 366
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 2 cut(s) 27, 367
Bme1390I CCNGG 2 cut(s) 596, 999
Bme18I GGWCC 2 cut(s) 592, 656
BmeT110I CYCGRG 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 592, 656
BmiI GGNNCC 2 cut(s) 657, 678
BmrFI CCNGG 2 cut(s) 596, 999
BmrI ACTGGG 2 cut(s) 461, 743
BmsI GCATC 2 cut(s) 285, 490
BmuI ACTGGG 2 cut(s) 461, 743
BpmI CTGGAG 2 cut(s) 981, 1055
Bpu1102I GCTNAGC 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 935
BsaAI YACGTR 1 cut(s) 911
BsaBI GATNNNNATC 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 1216
BsaJI CCNNGG 2 cut(s) 86, 595
BsaWI WCCGGW 2 cut(s) 553, 1120
Bsc4I CCNNNNNNNGG 1 cut(s) 632
Bse1I ACTGG 4 cut(s) 456, 738, 835, 843
Bse21I CCTNAGG 2 cut(s) 681, 926
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 2 cut(s) 596, 999
BseDI CCNNGG 2 cut(s) 86, 595
BseGI GGATG 2 cut(s) 433, 481
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 1 cut(s) 632
BseMII CTCAG 1 cut(s) 695
BseNI ACTGG 4 cut(s) 456, 738, 835, 843
BseRI GAGGAG 2 cut(s) 178, 891
BseXI GCAGC 2 cut(s) 37, 377
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 2 cut(s) 721, 924
BsiEI CGRYCG 1 cut(s) 236
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 2 cut(s) 554, 1121
BslFI GGGAC 2 cut(s) 468, 669
BslI CCNNNNNNNGG 1 cut(s) 632
BsmAI GTCTC 3 cut(s) 125, 1000, 1216
BsmFI GGGAC 2 cut(s) 468, 669
BsnI GGCC 2 cut(s) 721, 924
Bso31I GGTCTC 1 cut(s) 1216
BsoBI CYCGRG 1 cut(s) 52
Bsp143I GATC 6 cut(s) 433, 676, 844, 1039, 1092, 1176
Bsp1720I GCTNAGC 1 cut(s) 21
BspACI CCGC 4 cut(s) 5, 110, 197, 816
BspANI GGCC 2 cut(s) 721, 924
BspCNI CTCAG 1 cut(s) 694
BspLI GGNNCC 2 cut(s) 657, 678
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 6 cut(s) 428, 671, 684, 852, 1047, 1184
BspTNI GGTCTC 1 cut(s) 1216
BsrI ACTGG 4 cut(s) 456, 738, 835, 843
BssECI CCNNGG 2 cut(s) 86, 595
BssMI GATC 6 cut(s) 433, 676, 844, 1039, 1092, 1176
Bst2UI CCWGG 2 cut(s) 596, 999
Bst4CI ACNGT 4 cut(s) 234, 263, 1031, 1189
Bst6I CTCTTC 8 cut(s) 149, 155, 328, 382, 733, 1083, 1164, 1239
BstAPI GCANNNNNTGC 1 cut(s) 1274
BstBAI YACGTR 1 cut(s) 911
BstC8I GCNNGC 2 cut(s) 335, 871
BstDEI CTNAG 6 cut(s) 21, 135, 681, 926, 1072, 1278
BstENI CCTNNNNNAGG 1 cut(s) 630
BstF5I GGATG 2 cut(s) 433, 481
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 6 cut(s) 436, 679, 847, 1042, 1095, 1179
BstMAI GTCTC 3 cut(s) 125, 1000, 1216
BstMBI GATC 6 cut(s) 433, 676, 844, 1039, 1092, 1176
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 5 cut(s) 295, 765, 879, 888, 1274
BstNI CCWGG 2 cut(s) 596, 999
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 2 cut(s) 594, 997
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 2 cut(s) 37, 377
BstX2I RGATCY 2 cut(s) 676, 844
BstYI RGATCY 2 cut(s) 676, 844
Bsu36I CCTNAGG 2 cut(s) 681, 926
BsuRI GGCC 2 cut(s) 721, 924
BtsCI GGATG 2 cut(s) 433, 481
BtsI GCAGTG 2 cut(s) 210, 942
BtsIMutI CAGTG 2 cut(s) 210, 942
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 2 cut(s) 335, 871
CaiI CAGNNNCTG 1 cut(s) 728
CfoI GCGC 1 cut(s) 365
Cfr13I GGNCC 2 cut(s) 592, 656
CseI GACGC 1 cut(s) 1185
Csp6I GTAC 2 cut(s) 354, 858
CviAII CATG 2 cut(s) 284, 478
CviQI GTAC 2 cut(s) 354, 858
DdeI CTNAG 6 cut(s) 21, 135, 681, 926, 1072, 1278
DpnI GATC 6 cut(s) 435, 678, 846, 1041, 1094, 1178
DpnII GATC 6 cut(s) 433, 676, 844, 1039, 1092, 1176
EaeI YGGCCR 1 cut(s) 719
Eam1104I CTCTTC 8 cut(s) 149, 155, 328, 382, 733, 1083, 1164, 1239
EarI CTCTTC 8 cut(s) 149, 155, 328, 382, 733, 1083, 1164, 1239
Eco147I AGGCCT 1 cut(s) 924
Eco31I GGTCTC 1 cut(s) 1216
Eco47I GGWCC 2 cut(s) 592, 656
Eco57I CTGAAG 1 cut(s) 1221
Eco72I CACGTG 1 cut(s) 911
Eco81I CCTNAGG 2 cut(s) 681, 926
Eco88I CYCGRG 1 cut(s) 52
EcoNI CCTNNNNNAGG 1 cut(s) 630
EcoO109I RGGNCCY 1 cut(s) 656
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 2 cut(s) 594, 997
EcoT22I ATGCAT 1 cut(s) 483
FaeI CATG 2 cut(s) 287, 481
FaqI GGGAC 2 cut(s) 468, 669
FatI CATG 2 cut(s) 283, 477
FauI CCCGC 2 cut(s) 103, 809
Fnu4HI GCNGC 2 cut(s) 26, 366
FokI GGATG 2 cut(s) 440, 468
Fsp4HI GCNGC 2 cut(s) 26, 366
FspBI CTAG 1 cut(s) 1010
GlaI GCGC 1 cut(s) 364
GluI GCNGC 2 cut(s) 26, 366
GsuI CTGGAG 2 cut(s) 981, 1055
HaeIII GGCC 2 cut(s) 721, 924
HapII CCGG 2 cut(s) 554, 1121
HgaI GACGC 1 cut(s) 1185
HhaI GCGC 1 cut(s) 365
Hin1II CATG 2 cut(s) 287, 481
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HinfI GANTC 6 cut(s) 46, 470, 688, 725, 1258, 1291
HpaII CCGG 2 cut(s) 554, 1121
HphI GGTGA 3 cut(s) 275, 845, 855
Hpy166II GTNNAC 3 cut(s) 619, 836, 940
Hpy188I TCNGA 5 cut(s) 302, 403, 730, 1240, 1296
Hpy188III TCNNGA 7 cut(s) 52, 311, 626, 650, 662, 952, 1034
Hpy8I GTNNAC 3 cut(s) 619, 836, 940
Hpy99I CGWCG 4 cut(s) 46, 263, 947, 1201
HpyAV CCTTC 6 cut(s) 92, 136, 219, 487, 1144, 1245
HpyCH4III ACNGT 4 cut(s) 234, 263, 1031, 1189
HpyCH4IV ACGT 3 cut(s) 860, 910, 1058
HpyCH4V TGCA 3 cut(s) 481, 613, 935
HpyF10VI GCNNNNNNNGC 5 cut(s) 295, 765, 879, 888, 1274
HpyF3I CTNAG 6 cut(s) 21, 135, 681, 926, 1072, 1278
HpySE526I ACGT 3 cut(s) 860, 910, 1058
Hsp92II CATG 2 cut(s) 287, 481
HspAI GCGC 1 cut(s) 363
Kzo9I GATC 6 cut(s) 433, 676, 844, 1039, 1092, 1176
LmnI GCTCC 1 cut(s) 1000
Lsp1109I GCAGC 2 cut(s) 37, 377
LweI GCATC 2 cut(s) 285, 490
MaeI CTAG 1 cut(s) 1010
MaeII ACGT 3 cut(s) 860, 910, 1058
MaeIII GTNAC 4 cut(s) 263, 371, 819, 861
MalI GATC 6 cut(s) 435, 678, 846, 1041, 1094, 1178
MboI GATC 6 cut(s) 433, 676, 844, 1039, 1092, 1176
MfeI CAATTG 1 cut(s) 769
MflI RGATCY 2 cut(s) 676, 844
MlsI TGGCCA 1 cut(s) 721
MluCI AATT 8 cut(s) 105, 121, 404, 412, 712, 769, 792, 1154
MluNI TGGCCA 1 cut(s) 721
MlyI GAGTC 1 cut(s) 682
MmeI TCCRAC 2 cut(s) 400, 509
Mox20I TGGCCA 1 cut(s) 721
Mph1103I ATGCAT 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 515
MscI TGGCCA 1 cut(s) 721
MseI TTAA 4 cut(s) 798, 810, 1157, 1283
Msp20I TGGCCA 1 cut(s) 721
MspA1I CMGCKG 2 cut(s) 28, 368
MspI CCGG 2 cut(s) 554, 1121
MspR9I CCNGG 2 cut(s) 596, 999
MunI CAATTG 1 cut(s) 769
MvaI CCWGG 2 cut(s) 596, 999
MwoI GCNNNNNNNGC 5 cut(s) 295, 765, 879, 888, 1274
NdeII GATC 6 cut(s) 433, 676, 844, 1039, 1092, 1176
NlaIII CATG 2 cut(s) 287, 481
NlaIV GGNNCC 2 cut(s) 657, 678
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 3 cut(s) 263, 371, 861
NsiI ATGCAT 1 cut(s) 483
NspI RCATGY 1 cut(s) 287
PaeR7I CTCGAG 1 cut(s) 52
PceI AGGCCT 1 cut(s) 924
PciI ACATGT 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 515
PfeI GAWTC 5 cut(s) 46, 470, 725, 1258, 1291
PflFI GACNNNGTC 1 cut(s) 232
PkrI GCNGC 2 cut(s) 27, 367
PleI GAGTC 1 cut(s) 682
PmaCI CACGTG 1 cut(s) 911
PmlI CACGTG 1 cut(s) 911
PpsI GAGTC 1 cut(s) 682
Ppu21I YACGTR 1 cut(s) 911
PpuMI RGGWCCY 1 cut(s) 656
PscI ACATGT 1 cut(s) 283
Psp1406I AACGTT 1 cut(s) 1058
Psp5II RGGWCCY 1 cut(s) 656
Psp6I CCWGG 2 cut(s) 594, 997
PspCI CACGTG 1 cut(s) 911
PspGI CCWGG 2 cut(s) 594, 997
PspN4I GGNNCC 2 cut(s) 657, 678
PspPI GGNCC 2 cut(s) 592, 656
PspPPI RGGWCCY 1 cut(s) 656
PsrI GAACNNNNNNTAC 2 cut(s) 506, 538
PstNI CAGNNNCTG 1 cut(s) 728
PsuI RGATCY 2 cut(s) 676, 844
PsyI GACNNNGTC 1 cut(s) 232
PvuII CAGCTG 2 cut(s) 28, 368
RsaI GTAC 2 cut(s) 355, 859
RsaNI GTAC 2 cut(s) 354, 858
SaqAI TTAA 4 cut(s) 798, 810, 1157, 1283
SatI GCNGC 2 cut(s) 26, 366
Sau3AI GATC 6 cut(s) 433, 676, 844, 1039, 1092, 1176
Sau96I GGNCC 2 cut(s) 592, 656
SchI GAGTC 1 cut(s) 682
ScrFI CCNGG 2 cut(s) 596, 999
SfaNI GCATC 2 cut(s) 285, 490
SfcI CTRYAG 1 cut(s) 501
Sfr274I CTCGAG 1 cut(s) 52
SinI GGWCC 2 cut(s) 592, 656
SlaI CTCGAG 1 cut(s) 52
SmlI CTYRAG 2 cut(s) 52, 950
SmoI CTYRAG 2 cut(s) 52, 950
Sse9I AATT 8 cut(s) 105, 121, 404, 412, 712, 769, 792, 1154
SseBI AGGCCT 1 cut(s) 924
SsiI CCGC 4 cut(s) 5, 110, 197, 816
SspMI CTAG 1 cut(s) 1010
StuI AGGCCT 1 cut(s) 924
StyD4I CCNGG 2 cut(s) 594, 997
TaaI ACNGT 4 cut(s) 234, 263, 1031, 1189
TaiI ACGT 3 cut(s) 863, 913, 1061
TaqI TCGA 9 cut(s) 44, 53, 125, 236, 805, 984, 1065, 1134, 1199
TasI AATT 8 cut(s) 105, 121, 404, 412, 712, 769, 792, 1154
TatI WGTACW 1 cut(s) 353
TfiI GAWTC 5 cut(s) 46, 470, 725, 1258, 1291
Tru1I TTAA 4 cut(s) 798, 810, 1157, 1283
Tru9I TTAA 4 cut(s) 798, 810, 1157, 1283
TscAI CASTG 2 cut(s) 210, 942
TseFI GTSAC 3 cut(s) 263, 371, 861
TseI GCWGC 2 cut(s) 25, 365
Tsp45I GTSAC 3 cut(s) 263, 371, 861
TspDTI ATGAA 2 cut(s) 264, 452
TspGWI ACGGA 3 cut(s) 264, 316, 1113
TspRI CASTG 2 cut(s) 210, 942
Tth111I GACNNNGTC 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 592, 656
XagI CCTNNNNNAGG 1 cut(s) 630
XapI RAATTY 2 cut(s) 412, 712
XceI RCATGY 1 cut(s) 287
XhoI CTCGAG 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 515
XspI CTAG 1 cut(s) 1010
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.