FvH4_2g30630

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010420
Physical Location & Seq
Reverse (-)
23689047 .. 23691944
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30630.t1

Sequence Viewer

Length: 1269 bp
ATGGCAACTGCTTCGCTATCAACTCCGGCTTCAAGAATCTTCTCATTCTCTTCCTTATCAATCCGCCTCCGAACCCTCACAACCGCCGCACGCGCCACCACCACCACCACTAGTAGTAAGAAGAAGCAGCAGCAGAAGCACAAGGAGCACAAGCAGAGAGTTGCCGTCAAAGAAAAGCGAAGAACTCGCTCCGATCGAGAGTTCGAATTGGAGACCATCAAGCGTTATGGCGACACCTCTTTCCACATACCTGTCCTCCTCGGCGAAGTCCTCGACGTTTTTCCCGCCTCCAAACCCCTCCGCCGTTTCGTCGACTGCACCCTCGGAGCCGCCGGCCACTCCTCCGCCATAATTGAAGGGCATCCCGAAATGGAAGTGTATGTGGGGCTTGATGTTGATCCTGTGGCGCTTGAAAAGGCTCGGGGTCGGATCAATGGTGTGTTGGACGCCCCTTTGAAAGCTTATACATTCTTGGAGAATTTCAGATGCATCAAGTCTTTGCTTGCTGACGTGGATGAGACGCTTCTCGACGCTGGGATTGATGGCATCTTGATGGATTTGGGGATGTCATCGATGCAGGTGAATGATCCTCAGAGAGGGTTCAGTGTGCTTGCTGATGGGCCTCTCGATATGCGCATGGATCCTCAGGCAAGTCTGAAAGCAGAAGACATTTTAAACTCTTGGCCAGAAACTGAGGTAGGGCGCATCCTGCGAGAGTATGGGGAAGAAAGCAACTGGTACTCTCTTCAGAATAAAATAGTTAAAGCACGATTACAAGGTGGACTGCATTCCACTGGTGAACTAGTAGATCTTATAAAATCTGCAACCCCTCTAAGTCGAGAAGGGAGGCAAGGTTGGATTAAGACAGCAACAAGGGTGTTTCAAGCTCTGAGGATAGCAGTTAATGATGAGTTGAAGACTCTGGAGGATTCTCTATATTCATGTTTCGATTGCCTTGCACCTGGTGGTAGGCTGGCAGTCATCTCTTTCCACAGTTTAGAGGACAGAATTGTGAAGCAAACATTTCTTGACATCATCAACATCGAAAGCAGAGATGGGGATGCTGATATGATTGAAGAAGAAGCACAGAAGAAAGATTTGAGAAAGATCAGAATAGACATTGATGAAGATGAATCGTGGATCAAACAAATGATACAAGGGTTCAGAGGAACAATTCTCACTAAGAGACCAATTACGCCATCTGAACAGGAAGAGAAACTGAACACCCGGTCTAGGAGTGCGAAGCTTAGGGTGATTCAGAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

423

Amino Acids

47.4

Weight (kDa)

7.07

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 80 - 421 8.9e-87 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 815
AarI CACCTGC 1 cut(s) 568
Acc16I TGCGCA 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 568
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 93
AciI CCGC 7 cut(s) 64, 84, 87, 285, 301, 330, 345
AclWI GGATC 6 cut(s) 392, 437, 581, 635, 648, 1148
AcoI YGGCCR 2 cut(s) 334, 683
AcsI RAATTY 1 cut(s) 478
AcuI CTGAAG 1 cut(s) 731
AcyI GRCGYC 1 cut(s) 447
AdeI CACNNNGTG 1 cut(s) 965
AfaI GTAC 1 cut(s) 740
AfiI CCNNNNNNNGG 2 cut(s) 596, 1233
AgsI TTSAA 7 cut(s) 33, 356, 413, 457, 884, 916, 1076
AhlI ACTAGT 2 cut(s) 110, 802
AjiI CACGTC 1 cut(s) 511
AjnI CCWGG 1 cut(s) 961
AluBI AGCT 3 cut(s) 461, 887, 1246
AluI AGCT 3 cut(s) 461, 887, 1246
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 3 cut(s) 206, 512, 1180
AlwI GGATC 6 cut(s) 392, 437, 581, 635, 648, 1148
AlwNI CAGNNNCTG 1 cut(s) 692
Ama87I CYCGRG 1 cut(s) 420
AoxI GGCC 3 cut(s) 334, 620, 683
ApeKI GCWGC 2 cut(s) 127, 130
ApoI RAATTY 1 cut(s) 478
AspLEI GCGC 4 cut(s) 95, 409, 636, 705
AspS9I GGNCC 1 cut(s) 620
AsuC2I CCSGG 1 cut(s) 1228
AsuHPI GGTGA 3 cut(s) 592, 809, 1264
AsuII TTCGAA 1 cut(s) 204
AvaI CYCGRG 1 cut(s) 420
AxyI CCTNAGG 1 cut(s) 645
BaeI ACNNNNGTAYC 2 cut(s) 1145, 1178
BalI TGGCCA 1 cut(s) 685
BamHI GGATCC 1 cut(s) 640
BbsI GAAGAC 2 cut(s) 672, 923
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 139, 142
BccI CCATC 6 cut(s) 224, 536, 547, 611, 1049, 1207
BceAI ACGGC 2 cut(s) 149, 288
BcgI CGANNNNNNTGC 3 cut(s) 28, 938, 972
BciT130I CCWGG 1 cut(s) 963
BcnI CCSGG 1 cut(s) 1228
BcoDI GTCTC 3 cut(s) 206, 512, 1180
BcuI ACTAGT 2 cut(s) 110, 802
BfaI CTAG 3 cut(s) 111, 803, 1233
BfoI RGCGCY 1 cut(s) 410
BfuAI ACCTGC 1 cut(s) 568
BglII AGATCT 1 cut(s) 808
BisI GCNGC 4 cut(s) 87, 128, 131, 330
BlsI GCNGC 4 cut(s) 88, 129, 132, 331
Bme1390I CCNGG 2 cut(s) 963, 1228
BmeT110I CYCGRG 1 cut(s) 420
BmgBI CACGTC 1 cut(s) 511
BmgT120I GGNCC 1 cut(s) 620
BmiI GGNNCC 2 cut(s) 328, 642
BmrFI CCNGG 2 cut(s) 963, 1228
BmsI GCATC 7 cut(s) 370, 476, 498, 555, 564, 714, 1051
BpiI GAAGAC 2 cut(s) 672, 923
BpmI CTGGAG 1 cut(s) 944
Bpu10I CCTNAGC 1 cut(s) 1247
Bpu14I TTCGAA 1 cut(s) 204
BpuMI CCSGG 1 cut(s) 1228
Bsa29I ATCGAT 1 cut(s) 572
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 447
BsaI GGTCTC 2 cut(s) 206, 1180
BsaJI CCNNGG 2 cut(s) 259, 322
BsaXI ACNNNNNCTCC 4 cut(s) 240, 270, 838, 868
Bsc4I CCNNNNNNNGG 2 cut(s) 596, 1233
Bse118I RCCGGY 1 cut(s) 332
Bse1I ACTGG 2 cut(s) 740, 799
Bse21I CCTNAGG 1 cut(s) 645
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 1 cut(s) 963
BseCI ATCGAT 1 cut(s) 572
BseDI CCNNGG 2 cut(s) 259, 322
BseGI GGATG 5 cut(s) 361, 520, 570, 705, 1066
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 2 cut(s) 596, 1233
BseMII CTCAG 4 cut(s) 605, 659, 684, 881
BseNI ACTGG 2 cut(s) 740, 799
BseRI GAGGAG 2 cut(s) 248, 331
BseXI GCAGC 2 cut(s) 139, 142
BseYI CCCAGC 1 cut(s) 533
BsgI GTGCAG 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 3 cut(s) 336, 622, 685
BshVI ATCGAT 1 cut(s) 572
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 3 cut(s) 26, 333, 1228
BslI CCNNNNNNNGG 2 cut(s) 596, 1233
BsmAI GTCTC 3 cut(s) 206, 512, 1180
BsmBI CGTCTC 1 cut(s) 512
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 3 cut(s) 336, 622, 685
Bso31I GGTCTC 2 cut(s) 206, 1180
BsoBI CYCGRG 1 cut(s) 420
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 8 cut(s) 193, 397, 429, 586, 640, 808, 1107, 1140
BspACI CCGC 7 cut(s) 64, 84, 87, 285, 301, 330, 345
BspANI GGCC 3 cut(s) 336, 622, 685
BspCNI CTCAG 4 cut(s) 604, 658, 685, 882
BspDI ATCGAT 1 cut(s) 572
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 328, 642
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 6 cut(s) 392, 437, 581, 635, 648, 1148
BspT104I TTCGAA 1 cut(s) 204
BspTNI GGTCTC 2 cut(s) 206, 1180
BsrFI RCCGGY 1 cut(s) 332
BsrI ACTGG 2 cut(s) 740, 799
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 2 cut(s) 259, 322
BssMI GATC 8 cut(s) 193, 397, 429, 586, 640, 808, 1107, 1140
BssNI GRCGYC 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 963
Bst4CI ACNGT 1 cut(s) 995
Bst6I CTCTTC 3 cut(s) 55, 750, 1206
BstACI GRCGYC 1 cut(s) 447
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 5 cut(s) 91, 334, 504, 612, 975
BstDEI CTNAG 7 cut(s) 591, 645, 693, 833, 890, 1182, 1247
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 5 cut(s) 361, 520, 570, 705, 1066
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 4 cut(s) 95, 409, 636, 705
BstKTI GATC 8 cut(s) 196, 400, 432, 589, 643, 811, 1110, 1143
BstMAI GTCTC 3 cut(s) 206, 512, 1180
BstMBI GATC 8 cut(s) 193, 397, 429, 586, 640, 808, 1107, 1140
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 4 cut(s) 92, 136, 145, 709
BstNI CCWGG 1 cut(s) 963
BstSCI CCNGG 2 cut(s) 961, 1226
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 139, 142
BstV2I GAAGAC 2 cut(s) 672, 923
BstX2I RGATCY 2 cut(s) 640, 808
BstYI RGATCY 2 cut(s) 640, 808
Bsu15I ATCGAT 1 cut(s) 572
Bsu36I CCTNAGG 1 cut(s) 645
BsuRI GGCC 3 cut(s) 336, 622, 685
BsuTUI ATCGAT 1 cut(s) 572
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 5 cut(s) 361, 520, 570, 705, 1066
BtsIMutI CAGTG 2 cut(s) 610, 792
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 5 cut(s) 91, 334, 504, 612, 975
CaiI CAGNNNCTG 1 cut(s) 692
CfoI GCGC 4 cut(s) 95, 409, 636, 705
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 620
ClaI ATCGAT 1 cut(s) 572
CseI GACGC 3 cut(s) 455, 529, 539
CsiI ACCWGGT 1 cut(s) 961
Csp6I GTAC 1 cut(s) 739
CviAII CATG 2 cut(s) 637, 942
CviQI GTAC 1 cut(s) 739
DdeI CTNAG 7 cut(s) 591, 645, 693, 833, 890, 1182, 1247
DpnI GATC 8 cut(s) 195, 399, 431, 588, 642, 810, 1109, 1142
DpnII GATC 8 cut(s) 193, 397, 429, 586, 640, 808, 1107, 1140
DraI TTTAAA 1 cut(s) 675
DraIII CACNNNGTG 1 cut(s) 965
EaeI YGGCCR 2 cut(s) 334, 683
Eam1104I CTCTTC 3 cut(s) 55, 750, 1206
EarI CTCTTC 3 cut(s) 55, 750, 1206
EciI GGCGGA 3 cut(s) 53, 290, 334
Eco31I GGTCTC 2 cut(s) 206, 1180
Eco57I CTGAAG 1 cut(s) 731
Eco81I CCTNAGG 1 cut(s) 645
Eco88I CYCGRG 1 cut(s) 420
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoRII CCWGG 1 cut(s) 961
EcoT22I ATGCAT 1 cut(s) 491
Esp3I CGTCTC 1 cut(s) 512
FaeI CATG 2 cut(s) 640, 945
FatI CATG 2 cut(s) 636, 941
FauI CCCGC 1 cut(s) 292
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 4 cut(s) 87, 128, 131, 330
FokI GGATG 5 cut(s) 348, 527, 577, 692, 1073
Fsp4HI GCNGC 4 cut(s) 87, 128, 131, 330
FspAI RTGCGCAY 1 cut(s) 635
FspBI CTAG 3 cut(s) 111, 803, 1233
FspI TGCGCA 1 cut(s) 635
GlaI GCGC 4 cut(s) 94, 408, 635, 704
GluI GCNGC 4 cut(s) 87, 128, 131, 330
GsaI CCCAGC 1 cut(s) 537
GsuI CTGGAG 1 cut(s) 944
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 3 cut(s) 336, 622, 685
HapII CCGG 3 cut(s) 26, 333, 1228
HgaI GACGC 3 cut(s) 455, 529, 539
HhaI GCGC 4 cut(s) 95, 409, 636, 705
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 2 cut(s) 640, 945
Hin6I GCGC 4 cut(s) 93, 407, 634, 703
HinP1I GCGC 4 cut(s) 93, 407, 634, 703
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 2 cut(s) 459, 1244
HinfI GANTC 5 cut(s) 36, 919, 929, 1133, 1255
HpaII CCGG 3 cut(s) 26, 333, 1228
HphI GGTGA 3 cut(s) 592, 809, 1264
Hpy166II GTNNAC 3 cut(s) 313, 782, 800
Hpy188III TCNNGA 9 cut(s) 33, 197, 365, 527, 550, 626, 839, 923, 1028
Hpy8I GTNNAC 3 cut(s) 313, 782, 800
Hpy99I CGWCG 3 cut(s) 278, 314, 533
HpyAV CCTTC 3 cut(s) 350, 836, 1255
HpyCH4III ACNGT 1 cut(s) 995
HpyCH4IV ACGT 2 cut(s) 276, 510
HpyCH4V TGCA 6 cut(s) 318, 489, 577, 787, 824, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 136, 145, 709
HpyF3I CTNAG 7 cut(s) 591, 645, 693, 833, 890, 1182, 1247
HpySE526I ACGT 2 cut(s) 276, 510
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 2 cut(s) 640, 945
HspAI GCGC 4 cut(s) 93, 407, 634, 703
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
Kzo9I GATC 8 cut(s) 193, 397, 429, 586, 640, 808, 1107, 1140
LmnI GCTCC 3 cut(s) 145, 194, 326
Lsp1109I GCAGC 2 cut(s) 139, 142
LweI GCATC 7 cut(s) 370, 476, 498, 555, 564, 714, 1051
MabI ACCWGGT 1 cut(s) 961
MaeI CTAG 3 cut(s) 111, 803, 1233
MaeII ACGT 2 cut(s) 276, 510
MalI GATC 8 cut(s) 195, 399, 431, 588, 642, 810, 1109, 1142
MboI GATC 8 cut(s) 193, 397, 429, 586, 640, 808, 1107, 1140
MflI RGATCY 2 cut(s) 640, 808
MhlI GDGCHC 1 cut(s) 150
MlsI TGGCCA 1 cut(s) 685
MluCI AATT 6 cut(s) 206, 351, 478, 1008, 1173, 1191
MluNI TGGCCA 1 cut(s) 685
MlyI GAGTC 1 cut(s) 913
MmeI TCCRAC 3 cut(s) 407, 423, 836
Mox20I TGGCCA 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 491
MroNI GCCGGC 1 cut(s) 332
MscI TGGCCA 1 cut(s) 685
MseI TTAA 4 cut(s) 674, 762, 861, 903
MslI CAYNNNNRTG 1 cut(s) 551
Msp20I TGGCCA 1 cut(s) 685
MspI CCGG 3 cut(s) 26, 333, 1228
MspR9I CCNGG 2 cut(s) 963, 1228
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 1 cut(s) 963
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 4 cut(s) 92, 136, 145, 709
NaeI GCCGGC 1 cut(s) 334
NciI CCSGG 1 cut(s) 1228
NdeII GATC 8 cut(s) 193, 397, 429, 586, 640, 808, 1107, 1140
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 2 cut(s) 640, 945
NlaIV GGNNCC 2 cut(s) 328, 642
NmeAIII GCCGAG 1 cut(s) 240
NsbI TGCGCA 1 cut(s) 635
NsiI ATGCAT 1 cut(s) 491
NspV TTCGAA 1 cut(s) 204
PaqCI CACCTGC 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PctI GAATGC 1 cut(s) 787
PdiI GCCGGC 1 cut(s) 334
PfeI GAWTC 4 cut(s) 36, 929, 1133, 1255
PkrI GCNGC 4 cut(s) 88, 129, 132, 331
Ple19I CGATCG 1 cut(s) 196
PleI GAGTC 1 cut(s) 913
PpsI GAGTC 1 cut(s) 913
PsiI TTATAA 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 961
PspFI CCCAGC 1 cut(s) 533
PspGI CCWGG 1 cut(s) 961
PspN4I GGNNCC 2 cut(s) 328, 642
PspPI GGNCC 1 cut(s) 620
PstNI CAGNNNCTG 1 cut(s) 692
PsuI RGATCY 2 cut(s) 640, 808
PvuI CGATCG 1 cut(s) 196
RsaI GTAC 1 cut(s) 740
RsaNI GTAC 1 cut(s) 739
RseI CAYNNNNRTG 1 cut(s) 551
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 4 cut(s) 674, 762, 861, 903
SatI GCNGC 4 cut(s) 87, 128, 131, 330
Sau3AI GATC 8 cut(s) 193, 397, 429, 586, 640, 808, 1107, 1140
Sau96I GGNCC 1 cut(s) 620
SchI GAGTC 1 cut(s) 913
ScrFI CCNGG 2 cut(s) 963, 1228
SduI GDGCHC 1 cut(s) 150
SexAI ACCWGGT 1 cut(s) 961
SfaNI GCATC 7 cut(s) 370, 476, 498, 555, 564, 714, 1051
SfuI TTCGAA 1 cut(s) 204
SmiMI CAYNNNNRTG 1 cut(s) 551
SpeI ACTAGT 2 cut(s) 110, 802
Sse9I AATT 6 cut(s) 206, 351, 478, 1008, 1173, 1191
SsiI CCGC 7 cut(s) 64, 84, 87, 285, 301, 330, 345
SspMI CTAG 3 cut(s) 111, 803, 1233
StyD4I CCNGG 2 cut(s) 961, 1226
TaaI ACNGT 1 cut(s) 995
TaiI ACGT 2 cut(s) 279, 513
TasI AATT 6 cut(s) 206, 351, 478, 1008, 1173, 1191
TauI GCSGC 2 cut(s) 89, 332
TfiI GAWTC 4 cut(s) 36, 929, 1133, 1255
Tru1I TTAA 4 cut(s) 674, 762, 861, 903
Tru9I TTAA 4 cut(s) 674, 762, 861, 903
TscAI CASTG 2 cut(s) 610, 799
TseI GCWGC 2 cut(s) 127, 130
TspDTI ATGAA 3 cut(s) 930, 1140, 1146
TspRI CASTG 2 cut(s) 610, 799
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 3 cut(s) 111, 803, 1233
Zsp2I ATGCAT 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.