Rroxscaffold_7G00156870

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010420
Physical Location & Seq
Reverse (-)
780652 .. 783208
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00156870.1

Sequence Viewer

Length: 1281 bp
ATGGCAACAGCTGCTTCACTATCAACTCCTGCATCAAGAATCTTCTCTCTCTCTTCCTTCTCACTCCGCCTCCGAAGCCTCACATCCGCCGCCCACAGTCTCAGTCGGAGCACCACTACCACCACGACTAGTAAGAAGAAGAAGCAGCAGCAGCAGAAGCAGAAGCACAGAGTCGCCGTCAAAGAAAAGAGAAGAACTCGCTCCGACCGAGAGTTCGACATCAACCGTTATGGAGACTCCGCTTTCCACATCCCAGTCCTGCTCGGCGAAGTCATCGACGTTTTCCCGCCCTCCAAACCCCTCCGGCGTTTCGTCGACTGCACCGTCGGAGCTGCCGGCCACTCTTCCGCGATAATTGAAGGGCATCCTGAAATGGAAGTGTATGTGGGACTTGATGTTGATCCTGTGGCGCTTGACAAGGCTCGAGCTCGGATCAATTCTGTGTTGAACGACCCAAGTTCGACCCTGAAAGCTTATACATTTTTGGAGAATTTTAGATACATCAAGTCTTTGCTTACTGACGTGGATGAGACACTCCTTGACACTGGAATTGATGGCATTTTGATGGATTTAGGAATGTCGTCGATGCAGGTGAATGATCCTCAGAGAGGGTTCAGTGTACTTGCAAATGGGCCTCTTGATATGCGCATGGATCCTCAGGCGAGTCTGAAAGCAGAAGACATTTTAAACTCTTGGCCAGAAACTGAGGTGGGGCGCATCCTGCGAGAGTATGGGGAAGAAAGCAATTGGTACTCTCTTCAAAACAAAATAGTTAAAGCACGCTTACAAGGTGGATTGCATTCCACTGGTGAACTAGTAGATCTTATAAAATCTGCAACTCCTCTAAGTCGAGAAGGGAGGCAAGGTTGGATTAAGACAGCAACAAGGGTGTTTCAAGCTCTGAGGATAGCAGTTAACGATGAGTTGAAGACTCTGGAGGATTCTCTTTATTCATGCTTTGACTGCCTGGCACCTGGTGGTAGGCTAGCGGTCATCTCTTTCCACAGTTTGGAGGACAGAATTGTGAAACAAACATTTCTTGACATCATCAACATCAAAGGCGGAGATGGGGACGCTTACATGATTGAAGAAAGAGAGCGGAAGCAAGATTTGAGAAAGATCAGAATAGACATTGATGAAGACGAAATGTGGGTCAAACAAATGTTACAGGGCTTGAGAGGAACAATTCTCACTAAGAGACCAATTACGCCATCTGAAGAGGAAGAGAAACTGAACACCCGGTGTAGGAGTGCCAAGCTTAGGGTGATTCAGAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

426

Amino Acids

47.89

Weight (kDa)

8.7

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 81 - 425 4.4e-85 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 827
AarI CACCTGC 1 cut(s) 580
AasI GACNNNNNNGTC 1 cut(s) 323
Acc16I TGCGCA 1 cut(s) 647
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 970
AccB7I CCANNNNNTGG 1 cut(s) 1009
AccBSI CCGCTC 1 cut(s) 1099
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 350
AciI CCGC 9 cut(s) 67, 87, 90, 240, 287, 348, 989, 1062, 1099
AclWI GGATC 5 cut(s) 395, 440, 593, 647, 660
AcoI YGGCCR 2 cut(s) 337, 695
AcsI RAATTY 1 cut(s) 490
AcuI CTGAAG 1 cut(s) 1236
AdeI CACNNNGTG 2 cut(s) 977, 1242
AfaI GTAC 2 cut(s) 621, 752
AfiI CCNNNNNNNGG 4 cut(s) 608, 1009, 1245, 1260
AgsI TTSAA 6 cut(s) 359, 448, 761, 896, 928, 1088
AhlI ACTAGT 2 cut(s) 128, 814
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 2 cut(s) 966, 973
AluBI AGCT 6 cut(s) 11, 332, 428, 473, 899, 1258
AluI AGCT 6 cut(s) 11, 332, 428, 473, 899, 1258
Alw21I GWGCWC 2 cut(s) 113, 430
Alw26I GTCTC 4 cut(s) 104, 228, 524, 1192
AlwI GGATC 5 cut(s) 395, 440, 593, 647, 660
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 3 cut(s) 337, 632, 695
ApeKI GCWGC 5 cut(s) 11, 145, 148, 151, 332
ApoI RAATTY 1 cut(s) 490
ArsI GACNNNNNNTTYG 4 cut(s) 197, 229, 1137, 1169
AspLEI GCGC 3 cut(s) 412, 648, 717
AspS9I GGNCC 1 cut(s) 632
AsuC2I CCSGG 1 cut(s) 1240
AsuHPI GGTGA 3 cut(s) 604, 821, 1276
AsuNHI GCTAGC 1 cut(s) 985
AvaI CYCGRG 1 cut(s) 423
AxyI CCTNAGG 1 cut(s) 657
BalI TGGCCA 1 cut(s) 697
BamHI GGATCC 1 cut(s) 652
BanI GGYRCC 1 cut(s) 970
BanII GRGCYC 1 cut(s) 430
BbsI GAAGAC 3 cut(s) 684, 935, 1146
Bbv12I GWGCWC 2 cut(s) 113, 430
BbvI GCAGC 4 cut(s) 157, 160, 163, 319
BccI CCATC 4 cut(s) 548, 559, 1061, 1219
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 2 cut(s) 968, 975
BcnI CCSGG 1 cut(s) 1240
BcoDI GTCTC 4 cut(s) 104, 228, 524, 1192
BcuI ACTAGT 2 cut(s) 128, 814
BfaI CTAG 3 cut(s) 129, 815, 986
BfoI RGCGCY 1 cut(s) 413
BfuAI ACCTGC 1 cut(s) 580
BglII AGATCT 1 cut(s) 820
BisI GCNGC 6 cut(s) 12, 90, 146, 149, 152, 333
BlsI GCNGC 6 cut(s) 13, 91, 147, 150, 153, 334
Bme1390I CCNGG 3 cut(s) 968, 975, 1240
BmeT110I CYCGRG 1 cut(s) 423
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 1 cut(s) 632
BmiI GGNNCC 2 cut(s) 654, 972
BmrFI CCNGG 3 cut(s) 968, 975, 1240
BmrI ACTGGG 1 cut(s) 248
BmsI GCATC 4 cut(s) 41, 373, 576, 726
BmtI GCTAGC 1 cut(s) 989
BmuI ACTGGG 1 cut(s) 248
BpiI GAAGAC 3 cut(s) 684, 935, 1146
BplI GAGNNNNNCTC 2 cut(s) 181, 213
BpmI CTGGAG 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 1259
BpuEI CTTGAG 1 cut(s) 1195
BpuMI CCSGG 1 cut(s) 1240
BsaBI GATNNNNATC 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 1192
BsaXI ACNNNNNCTCC 4 cut(s) 54, 84, 850, 880
Bsc4I CCNNNNNNNGG 4 cut(s) 608, 1009, 1245, 1260
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 3 cut(s) 254, 550, 811
Bse21I CCTNAGG 1 cut(s) 657
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 2 cut(s) 968, 975
BseGI GGATG 5 cut(s) 83, 249, 364, 532, 717
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 4 cut(s) 608, 1009, 1245, 1260
BseMII CTCAG 5 cut(s) 115, 617, 671, 696, 893
BseNI ACTGG 3 cut(s) 254, 550, 811
BseRI GAGGAG 1 cut(s) 831
BseXI GCAGC 4 cut(s) 157, 160, 163, 319
BsgI GTGCAG 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 350
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 3 cut(s) 339, 634, 697
BshNI GGYRCC 1 cut(s) 970
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 113, 430
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 3 cut(s) 304, 336, 1240
BslFI GGGAC 2 cut(s) 402, 1085
BslI CCNNNNNNNGG 4 cut(s) 608, 1009, 1245, 1260
BsmAI GTCTC 4 cut(s) 104, 228, 524, 1192
BsmFI GGGAC 2 cut(s) 402, 1085
BsmI GAATGC 1 cut(s) 799
BsnI GGCC 3 cut(s) 339, 634, 697
Bso31I GGTCTC 1 cut(s) 1192
BsoBI CYCGRG 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 113, 430
Bsp143I GATC 6 cut(s) 400, 432, 598, 652, 820, 1119
BspACI CCGC 9 cut(s) 67, 87, 90, 240, 287, 348, 989, 1062, 1099
BspANI GGCC 3 cut(s) 339, 634, 697
BspCNI CTCAG 5 cut(s) 114, 616, 670, 697, 894
BspFNI CGCG 1 cut(s) 350
BspLI GGNNCC 2 cut(s) 654, 972
BspMI ACCTGC 1 cut(s) 580
BspOI GCTAGC 1 cut(s) 989
BspPI GGATC 5 cut(s) 395, 440, 593, 647, 660
BspT107I GGYRCC 1 cut(s) 970
BspTNI GGTCTC 1 cut(s) 1192
BsrBI CCGCTC 1 cut(s) 1099
BsrFI RCCGGY 1 cut(s) 335
BsrI ACTGG 3 cut(s) 254, 550, 811
BssAI RCCGGY 1 cut(s) 335
BssMI GATC 6 cut(s) 400, 432, 598, 652, 820, 1119
Bst2UI CCWGG 2 cut(s) 968, 975
Bst4CI ACNGT 4 cut(s) 98, 227, 325, 1007
Bst6I CTCTTC 5 cut(s) 58, 349, 762, 1212, 1218
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 337, 781, 987
BstDEI CTNAG 8 cut(s) 101, 603, 657, 705, 845, 902, 1194, 1259
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 5 cut(s) 83, 249, 364, 532, 717
BstFNI CGCG 1 cut(s) 350
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 3 cut(s) 412, 648, 717
BstKTI GATC 6 cut(s) 403, 435, 601, 655, 823, 1122
BstMAI GTCTC 4 cut(s) 104, 228, 524, 1192
BstMBI GATC 6 cut(s) 400, 432, 598, 652, 820, 1119
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 6 cut(s) 11, 75, 151, 157, 721, 963
BstNI CCWGG 2 cut(s) 968, 975
BstSCI CCNGG 3 cut(s) 966, 973, 1238
BstUI CGCG 1 cut(s) 350
BstV1I GCAGC 4 cut(s) 157, 160, 163, 319
BstV2I GAAGAC 3 cut(s) 684, 935, 1146
BstX2I RGATCY 2 cut(s) 652, 820
BstYI RGATCY 2 cut(s) 652, 820
Bsu36I CCTNAGG 1 cut(s) 657
BsuRI GGCC 3 cut(s) 339, 634, 697
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 5 cut(s) 83, 249, 364, 532, 717
BtsIMutI CAGTG 3 cut(s) 543, 622, 804
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 3 cut(s) 337, 781, 987
CaiI CAGNNNCTG 1 cut(s) 704
CfoI GCGC 3 cut(s) 412, 648, 717
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 632
CseI GACGC 1 cut(s) 1082
CsiI ACCWGGT 1 cut(s) 973
Csp6I GTAC 2 cut(s) 620, 751
CviAII CATG 3 cut(s) 649, 954, 1081
CviQI GTAC 2 cut(s) 620, 751
DdeI CTNAG 8 cut(s) 101, 603, 657, 705, 845, 902, 1194, 1259
DpnI GATC 6 cut(s) 402, 434, 600, 654, 822, 1121
DpnII GATC 6 cut(s) 400, 432, 598, 652, 820, 1119
DraI TTTAAA 1 cut(s) 687
DraIII CACNNNGTG 2 cut(s) 977, 1242
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 2 cut(s) 337, 695
Eam1104I CTCTTC 5 cut(s) 58, 349, 762, 1212, 1218
EarI CTCTTC 5 cut(s) 58, 349, 762, 1212, 1218
EciI GGCGGA 3 cut(s) 56, 76, 1077
Ecl136II GAGCTC 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 1192
Eco53kI GAGCTC 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 1236
Eco81I CCTNAGG 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 423
EcoICRI GAGCTC 1 cut(s) 428
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoRII CCWGG 2 cut(s) 966, 973
EcoT38I GRGCYC 1 cut(s) 430
FaeI CATG 3 cut(s) 652, 957, 1084
FaiI YATR 9 cut(s) 231, 384, 477, 644, 650, 732, 827, 955, 1082
FaqI GGGAC 2 cut(s) 402, 1085
FatI CATG 3 cut(s) 648, 953, 1080
FauI CCCGC 1 cut(s) 294
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 6 cut(s) 12, 90, 146, 149, 152, 333
FokI GGATG 5 cut(s) 70, 236, 351, 539, 704
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 6 cut(s) 12, 90, 146, 149, 152, 333
FspAI RTGCGCAY 1 cut(s) 647
FspBI CTAG 3 cut(s) 129, 815, 986
FspI TGCGCA 1 cut(s) 647
GlaI GCGC 3 cut(s) 411, 647, 716
GluI GCNGC 6 cut(s) 12, 90, 146, 149, 152, 333
GsuI CTGGAG 1 cut(s) 956
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 3 cut(s) 339, 634, 697
HapII CCGG 3 cut(s) 304, 336, 1240
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 3 cut(s) 412, 648, 717
Hin1II CATG 3 cut(s) 652, 957, 1084
Hin6I GCGC 3 cut(s) 410, 646, 715
HinP1I GCGC 3 cut(s) 410, 646, 715
HincII GTYRAC 2 cut(s) 316, 916
HindII GTYRAC 2 cut(s) 316, 916
HindIII AAGCTT 2 cut(s) 471, 1256
HinfI GANTC 7 cut(s) 39, 171, 236, 664, 931, 941, 1267
HpaI GTTAAC 1 cut(s) 916
HpaII CCGG 3 cut(s) 304, 336, 1240
HphI GGTGA 3 cut(s) 604, 821, 1276
Hpy166II GTNNAC 4 cut(s) 316, 620, 812, 916
Hpy188III TCNNGA 6 cut(s) 36, 368, 638, 851, 935, 1040
Hpy8I GTNNAC 4 cut(s) 316, 620, 812, 916
Hpy99I CGWCG 4 cut(s) 281, 317, 329, 586
HpyAV CCTTC 4 cut(s) 67, 353, 848, 1267
HpyCH4III ACNGT 4 cut(s) 98, 227, 325, 1007
HpyCH4IV ACGT 2 cut(s) 279, 522
HpyCH4V TGCA 6 cut(s) 32, 321, 589, 626, 799, 836
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 75, 151, 157, 721, 963
HpyF3I CTNAG 8 cut(s) 101, 603, 657, 705, 845, 902, 1194, 1259
HpySE526I ACGT 2 cut(s) 279, 522
Hsp92II CATG 3 cut(s) 652, 957, 1084
HspAI GCGC 3 cut(s) 410, 646, 715
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
KspAI GTTAAC 1 cut(s) 916
Kzo9I GATC 6 cut(s) 400, 432, 598, 652, 820, 1119
LmnI GCTCC 3 cut(s) 108, 206, 329
Lsp1109I GCAGC 4 cut(s) 157, 160, 163, 319
LweI GCATC 4 cut(s) 41, 373, 576, 726
MabI ACCWGGT 1 cut(s) 973
MaeI CTAG 3 cut(s) 129, 815, 986
MaeII ACGT 2 cut(s) 279, 522
MaeIII GTNAC 1 cut(s) 1164
MalI GATC 6 cut(s) 402, 434, 600, 654, 822, 1121
MbiI CCGCTC 1 cut(s) 1099
MboI GATC 6 cut(s) 400, 432, 598, 652, 820, 1119
MfeI CAATTG 1 cut(s) 745
MflI RGATCY 2 cut(s) 652, 820
MhlI GDGCHC 2 cut(s) 113, 430
MlsI TGGCCA 1 cut(s) 697
MluCI AATT 8 cut(s) 354, 436, 490, 549, 745, 1020, 1185, 1203
MluNI TGGCCA 1 cut(s) 697
MlyI GAGTC 4 cut(s) 180, 230, 673, 925
MmeI TCCRAC 4 cut(s) 86, 228, 307, 848
Mox20I TGGCCA 1 cut(s) 697
MroNI GCCGGC 1 cut(s) 335
MscI TGGCCA 1 cut(s) 697
MseI TTAA 5 cut(s) 686, 774, 873, 915, 1279
MslI CAYNNNNRTG 1 cut(s) 563
Msp20I TGGCCA 1 cut(s) 697
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 3 cut(s) 304, 336, 1240
MspR9I CCNGG 3 cut(s) 968, 975, 1240
MunI CAATTG 1 cut(s) 745
Mva1269I GAATGC 1 cut(s) 799
MvaI CCWGG 2 cut(s) 968, 975
MvnI CGCG 1 cut(s) 350
MwoI GCNNNNNNNGC 6 cut(s) 11, 75, 151, 157, 721, 963
NaeI GCCGGC 1 cut(s) 337
NciI CCSGG 1 cut(s) 1240
NdeII GATC 6 cut(s) 400, 432, 598, 652, 820, 1119
NgoMIV GCCGGC 1 cut(s) 335
NheI GCTAGC 1 cut(s) 985
NlaIII CATG 3 cut(s) 652, 957, 1084
NlaIV GGNNCC 2 cut(s) 654, 972
NmeAIII GCCGAG 1 cut(s) 243
NsbI TGCGCA 1 cut(s) 647
PaeR7I CTCGAG 1 cut(s) 423
PaqCI CACCTGC 1 cut(s) 580
PcsI WCGNNNNNNNCGW 2 cut(s) 205, 321
PctI GAATGC 1 cut(s) 799
PdiI GCCGGC 1 cut(s) 337
PfeI GAWTC 3 cut(s) 39, 941, 1267
PflMI CCANNNNNTGG 1 cut(s) 1009
PkrI GCNGC 6 cut(s) 13, 91, 147, 150, 153, 334
PleI GAGTC 4 cut(s) 179, 230, 672, 925
PpsI GAGTC 4 cut(s) 179, 230, 672, 925
PsiI TTATAA 1 cut(s) 827
Psp124BI GAGCTC 1 cut(s) 430
Psp6I CCWGG 2 cut(s) 966, 973
PspGI CCWGG 2 cut(s) 966, 973
PspN4I GGNNCC 2 cut(s) 654, 972
PspPI GGNCC 1 cut(s) 632
PspXI VCTCGAGB 1 cut(s) 423
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 2 cut(s) 652, 820
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 621, 752
RsaNI GTAC 2 cut(s) 620, 751
RseI CAYNNNNRTG 1 cut(s) 563
SacI GAGCTC 1 cut(s) 430
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 5 cut(s) 686, 774, 873, 915, 1279
SatI GCNGC 6 cut(s) 12, 90, 146, 149, 152, 333
Sau3AI GATC 6 cut(s) 400, 432, 598, 652, 820, 1119
Sau96I GGNCC 1 cut(s) 632
SchI GAGTC 4 cut(s) 180, 230, 673, 925
ScrFI CCNGG 3 cut(s) 968, 975, 1240
SduI GDGCHC 2 cut(s) 113, 430
SexAI ACCWGGT 1 cut(s) 973
SfaNI GCATC 4 cut(s) 41, 373, 576, 726
Sfr274I CTCGAG 1 cut(s) 423
SlaI CTCGAG 1 cut(s) 423
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 2 cut(s) 423, 1174
SmoI CTYRAG 2 cut(s) 423, 1174
SpeI ACTAGT 2 cut(s) 128, 814
Sse9I AATT 8 cut(s) 354, 436, 490, 549, 745, 1020, 1185, 1203
SsiI CCGC 9 cut(s) 67, 87, 90, 240, 287, 348, 989, 1062, 1099
SspMI CTAG 3 cut(s) 129, 815, 986
SstI GAGCTC 1 cut(s) 430
StyD4I CCNGG 3 cut(s) 966, 973, 1238
TaaI ACNGT 4 cut(s) 98, 227, 325, 1007
TaiI ACGT 2 cut(s) 282, 525
TaqI TCGA 7 cut(s) 216, 276, 315, 424, 461, 584, 850
TaqII GACCGA 1 cut(s) 222
TasI AATT 8 cut(s) 354, 436, 490, 549, 745, 1020, 1185, 1203
TatI WGTACW 1 cut(s) 619
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 3 cut(s) 39, 941, 1267
Tru1I TTAA 5 cut(s) 686, 774, 873, 915, 1279
Tru9I TTAA 5 cut(s) 686, 774, 873, 915, 1279
TscAI CASTG 3 cut(s) 550, 622, 811
TseI GCWGC 5 cut(s) 11, 145, 148, 151, 332
TspDTI ATGAA 2 cut(s) 942, 1152
TspRI CASTG 3 cut(s) 550, 622, 811
Van91I CCANNNNNTGG 1 cut(s) 1009
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 1 cut(s) 490
XhoI CTCGAG 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 3 cut(s) 129, 815, 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.