MD08G1133300.v1.1

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010420
Physical Location & Seq
Reverse (-)
12666248 .. 12668136
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1133300.v1.1.491

Sequence Viewer

Length: 723 bp
ATGTTGATGGATTTGGGAATGTCGTCGATGCAAGTGAATGATCCACAAAGAGGGTTCAGTGTGCTTGCTAATGGACCTCTTGATATGAGGATGGATCCTCAGGCAAGTCTGAAAGCTGAAGACATTTTGAACTCTTGGCCAGATAATGAGGTAGGGCGCATCCTGCGGGACTATGGGGAAGAAAGTAACTGGTACTCTCTTCAAAATAGAATTGTTAGAGCCCGTTCACAGGGTGGATTGCATACCACTGGTGATTTAATACATCTTATAAAGAGTGCAACTCCGCTGAGTCGAGAAGGACGGCAAGGTTGGATTAAGACAGCTACAAGGGTGTTTCAAGCTCTAAGGATAGCCGTGAACGATGAACTGAAGACTCTCGAGGATTCTCTTTATTCTTCTTTTGACTGCCTTGCACCTGGTGGCAGGTTGGCAGTCATCTCTTTCCATAGTTTGGAGGACAGAATTGTGAAACAAACATTTCTGGACATCATCAACGAAAAAGACAGAGATGGTGATGTTGACATGAATGAAGAAGAACCACGGAAGAAAGATTTGAGAAAGATCATGAGTGACATTGATGAGAACGAAGTATGGATCAAACAAACGGTACAAGGCTTGAGAGGCATAATTCTCACTAAGAGACCAATTACACCGTCTGAAGCGGAAGAGAGCCTGAACAGCCGGGCTAGAAGTGCCAAGCTCAGGGTGCTTCAGAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

241

Amino Acids

27.23

Weight (kDa)

5.76

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 2 - 239 9.6e-64 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 414
AccB7I CCANNNNNTGG 1 cut(s) 451
AciI CCGC 3 cut(s) 166, 284, 662
AclWI GGATC 4 cut(s) 35, 89, 102, 602
AcoI YGGCCR 1 cut(s) 137
AcuI CTGAAG 4 cut(s) 138, 389, 678, 695
AdeI CACNNNGTG 2 cut(s) 233, 419
AfaI GTAC 2 cut(s) 194, 609
AfiI CCNNNNNNNGG 4 cut(s) 50, 229, 451, 702
AgsI TTSAA 3 cut(s) 130, 203, 338
AjnI CCWGG 1 cut(s) 415
AluBI AGCT 5 cut(s) 116, 323, 341, 700, 718
AluI AGCT 5 cut(s) 116, 323, 341, 700, 718
Alw26I GTCTC 1 cut(s) 634
AlwI GGATC 4 cut(s) 35, 89, 102, 602
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 1 cut(s) 137
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 683
AsuHPI GGTGA 2 cut(s) 263, 524
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 1 cut(s) 74
AxyI CCTNAGG 1 cut(s) 99
BalI TGGCCA 1 cut(s) 139
BamHI GGATCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 223
BbsI GAAGAC 2 cut(s) 126, 377
BccI CCATC 2 cut(s) 85, 503
BceAI ACGGC 2 cut(s) 317, 338
BciT130I CCWGG 1 cut(s) 417
BcnI CCSGG 1 cut(s) 683
BcoDI GTCTC 1 cut(s) 634
BfaI CTAG 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 414
Bme1390I CCNGG 2 cut(s) 417, 683
Bme18I GGWCC 1 cut(s) 74
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 2 cut(s) 417, 683
BmsI GCATC 2 cut(s) 18, 168
BpiI GAAGAC 2 cut(s) 126, 377
BplI GAGNNNNNCTC 2 cut(s) 265, 297
Bpu10I CCTNAGC 1 cut(s) 701
BpuEI CTTGAG 1 cut(s) 637
BpuMI CCSGG 1 cut(s) 683
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 1 cut(s) 539
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 229, 451, 702
Bse1I ACTGG 2 cut(s) 194, 253
Bse21I CCTNAGG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 2 cut(s) 96, 159
BseLI CCNNNNNNNGG 4 cut(s) 50, 229, 451, 702
BseMII CTCAG 3 cut(s) 113, 278, 715
BseNI ACTGG 2 cut(s) 194, 253
BshFI GGCC 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 682
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 4 cut(s) 50, 229, 451, 702
BsmAI GTCTC 1 cut(s) 634
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 1 cut(s) 139
Bso31I GGTCTC 1 cut(s) 634
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 4 cut(s) 40, 94, 561, 594
BspACI CCGC 3 cut(s) 166, 284, 662
BspANI GGCC 1 cut(s) 139
BspCNI CTCAG 3 cut(s) 112, 279, 714
BspHI TCATGA 1 cut(s) 564
BspLI GGNNCC 1 cut(s) 96
BspMI ACCTGC 1 cut(s) 414
BspPI GGATC 4 cut(s) 35, 89, 102, 602
BspTNI GGTCTC 1 cut(s) 634
BsrI ACTGG 2 cut(s) 194, 253
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 4 cut(s) 40, 94, 561, 594
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 2 cut(s) 607, 654
Bst6I CTCTTC 2 cut(s) 204, 660
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 5 cut(s) 99, 287, 344, 636, 701
BstDSI CCRYGG 1 cut(s) 539
BstF5I GGATG 2 cut(s) 96, 159
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 4 cut(s) 43, 97, 564, 597
BstMAI GTCTC 1 cut(s) 634
BstMBI GATC 4 cut(s) 40, 94, 561, 594
BstMWI GCNNNNNNNGC 6 cut(s) 163, 621, 678, 692, 706, 715
BstNI CCWGG 1 cut(s) 417
BstSCI CCNGG 2 cut(s) 415, 681
BstV2I GAAGAC 2 cut(s) 126, 377
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
Bsu36I CCTNAGG 1 cut(s) 99
BsuRI GGCC 1 cut(s) 139
BtgI CCRYGG 1 cut(s) 539
BtsCI GGATG 2 cut(s) 96, 159
BtsIMutI CAGTG 2 cut(s) 64, 246
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 1 cut(s) 564
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 74
CsiI ACCWGGT 1 cut(s) 415
Csp6I GTAC 2 cut(s) 193, 608
CviAII CATG 2 cut(s) 523, 565
CviQI GTAC 2 cut(s) 193, 608
DdeI CTNAG 5 cut(s) 99, 287, 344, 636, 701
DpnI GATC 4 cut(s) 42, 96, 563, 596
DpnII GATC 4 cut(s) 40, 94, 561, 594
DraIII CACNNNGTG 2 cut(s) 233, 419
EaeI YGGCCR 1 cut(s) 137
Eam1104I CTCTTC 2 cut(s) 204, 660
EarI CTCTTC 2 cut(s) 204, 660
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 74
Eco57I CTGAAG 4 cut(s) 138, 389, 678, 695
Eco81I CCTNAGG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 2 cut(s) 526, 568
FaiI YATR 9 cut(s) 86, 174, 243, 269, 447, 524, 566, 592, 626
FaqI GGGAC 1 cut(s) 182
FatI CATG 2 cut(s) 522, 564
FauI CCCGC 1 cut(s) 159
FokI GGATG 2 cut(s) 103, 146
FriOI GRGCYC 1 cut(s) 223
FspBI CTAG 1 cut(s) 687
GlaI GCGC 1 cut(s) 158
HaeIII GGCC 1 cut(s) 139
HapII CCGG 1 cut(s) 682
HhaI GCGC 1 cut(s) 159
Hin1II CATG 2 cut(s) 526, 568
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 3 cut(s) 289, 373, 383
HpaII CCGG 1 cut(s) 682
HphI GGTGA 2 cut(s) 263, 524
Hpy166II GTNNAC 3 cut(s) 227, 358, 520
Hpy188I TCNGA 3 cut(s) 111, 658, 714
Hpy188III TCNNGA 5 cut(s) 80, 293, 377, 482, 565
Hpy8I GTNNAC 3 cut(s) 227, 358, 520
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 2 cut(s) 607, 654
HpyCH4V TGCA 4 cut(s) 31, 241, 278, 413
HpyF10VI GCNNNNNNNGC 6 cut(s) 163, 621, 678, 692, 706, 715
HpyF3I CTNAG 5 cut(s) 99, 287, 344, 636, 701
Hsp92II CATG 2 cut(s) 526, 568
HspAI GCGC 1 cut(s) 157
Kzo9I GATC 4 cut(s) 40, 94, 561, 594
LweI GCATC 2 cut(s) 18, 168
MabI ACCWGGT 1 cut(s) 415
MaeI CTAG 1 cut(s) 687
MaeIII GTNAC 2 cut(s) 185, 569
MalI GATC 4 cut(s) 42, 96, 563, 596
MboI GATC 4 cut(s) 40, 94, 561, 594
MboII GAAGA 9 cut(s) 131, 191, 191, 382, 387, 542, 545, 556, 677
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 223
MlsI TGGCCA 1 cut(s) 139
MluCI AATT 4 cut(s) 210, 462, 627, 645
MluNI TGGCCA 1 cut(s) 139
MlyI GAGTC 2 cut(s) 298, 367
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 8 cut(s) 44, 81, 87, 108, 142, 373, 448, 614
Mox20I TGGCCA 1 cut(s) 139
MscI TGGCCA 1 cut(s) 139
MseI TTAA 3 cut(s) 257, 315, 721
Msp20I TGGCCA 1 cut(s) 139
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 2 cut(s) 417, 683
MvaI CCWGG 1 cut(s) 417
MwoI GCNNNNNNNGC 6 cut(s) 163, 621, 678, 692, 706, 715
NciI CCSGG 1 cut(s) 683
NdeII GATC 4 cut(s) 40, 94, 561, 594
NlaIII CATG 2 cut(s) 526, 568
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 569
PaeR7I CTCGAG 1 cut(s) 377
PagI TCATGA 1 cut(s) 564
PfeI GAWTC 1 cut(s) 383
PflMI CCANNNNNTGG 1 cut(s) 451
PleI GAGTC 2 cut(s) 297, 367
PpsI GAGTC 2 cut(s) 297, 367
PsiI TTATAA 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 415
PspGI CCWGG 1 cut(s) 415
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 1 cut(s) 74
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 2 cut(s) 194, 609
RsaNI GTAC 2 cut(s) 193, 608
SaqAI TTAA 3 cut(s) 257, 315, 721
Sau3AI GATC 4 cut(s) 40, 94, 561, 594
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 2 cut(s) 298, 367
ScrFI CCNGG 2 cut(s) 417, 683
SduI GDGCHC 1 cut(s) 223
SexAI ACCWGGT 1 cut(s) 415
SfaNI GCATC 2 cut(s) 18, 168
Sfr274I CTCGAG 1 cut(s) 377
SinI GGWCC 1 cut(s) 74
SlaI CTCGAG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 377, 616
SmoI CTYRAG 2 cut(s) 377, 616
Sse9I AATT 4 cut(s) 210, 462, 627, 645
SsiI CCGC 3 cut(s) 166, 284, 662
SspMI CTAG 1 cut(s) 687
StyD4I CCNGG 2 cut(s) 415, 681
TaaI ACNGT 2 cut(s) 607, 654
TaqI TCGA 3 cut(s) 26, 292, 378
TasI AATT 4 cut(s) 210, 462, 627, 645
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 3 cut(s) 257, 315, 721
Tru9I TTAA 3 cut(s) 257, 315, 721
TscAI CASTG 2 cut(s) 64, 253
TseFI GTSAC 1 cut(s) 569
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 3 cut(s) 378, 539, 543
TspGWI ACGGA 1 cut(s) 556
TspRI CASTG 2 cut(s) 64, 253
Van91I CCANNNNNTGG 1 cut(s) 451
VpaK11BI GGWCC 1 cut(s) 74
XhoI CTCGAG 1 cut(s) 377
XspI CTAG 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.