MD15G1110800.v1.1

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010420
Physical Location & Seq
Forward (+)
7743796 .. 7746394
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1110800.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGCTTGGCGAAGTTCTGGATGTTTTCCCGGCGTCCAAGCAACTCCGGTACTTCGTCGACTGCACTGTCGGAGCTGCCGGGCATTCCTCTGCGATAATTGAAGGGCATCCGGAAATGGAAGTGTATGTTGGGCTTGATGTCGATCCTGTGGCCCTTGAGAAGGCTCGAGCTCGGATCAATTCTGTTTTGCATGACCCGAATCCTTCGTCCGTCAAAGCTTACACCTTTTTGGAGAATTTCAGATATGTGAAGTCTCTGCTTTGTGATGTGGATGAGACACTAGTTGAAACGGGGATTGATGGGATACTGATGGATTTGGGAATGTCGTCGATGCAAGTAAATGATCCTCAAAGAGGGTTCAGTGTGCTTGCTAATGGACCTCTTGATATGAGGATGGATCCTCAGGCAAGTCTGAAAGCTGAAGACATTTTGAACTCTTGGCCAGATAATGAGGTAGGCCGCATCCTGCGAGACTATGGGGAAGAAAGCAACTGGTTCTCTCTTCAAAATAGAATTGTTAGAGCCCGATCACAGGGTGGATTGCATACCACTGGCGATTTAGTAGATCTTATAAAAGGTGCAACTCCGCTGAGTCGAGAAGGACGGCAAGGTTGGATTAAAACGGCTACAAGGGTGTTTCAAGCTCTGAGGATAGCCGTGAACGATGAACTGAAGACTCTGGAGGATTCTCTTTATGCTTCTTTTGACTGCCTTGCACCGGGTGGTAGGTTGGCAGTCATCTCTTTCCACAGTTTGGAGGACAGAATTGTGAAACAAACGTTTCTAGACATCATCAACGTCAAAGACAGAGATGTTGACGTTGACATGATTGAAGAAGCAGCGGGGAAGAAAGATTTGAGAAAGATCATGAGCGACATTGATGAAAACGAAGCATGGATCAAACAAACGGTACAGGGCTTGAGGGGAATAATTCTCACTAAGAGACCAATTACACCATCTGAAACGGAAGAGAGCCTCAACAGCCGAGCTAGAAGTGCCAAGCTTAGGGTGATTCAGAAGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

342

Amino Acids

37.97

Weight (kDa)

5.1

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 2 - 340 7.1e-81 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AasI GACNNNNNNGTC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 754
AccI GTMKAC 1 cut(s) 57
AccIII TCCGGA 1 cut(s) 109
AciI CCGC 3 cut(s) 460, 587, 842
AclI AACGTT 1 cut(s) 779
AclWI GGATC 6 cut(s) 137, 182, 338, 392, 405, 905
AcoI YGGCCR 1 cut(s) 440
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 441, 692
AcyI GRCGYC 1 cut(s) 32
AdeI CACNNNGTG 2 cut(s) 536, 722
AfaI GTAC 2 cut(s) 50, 912
AfiI CCNNNNNNNGG 6 cut(s) 160, 353, 532, 718, 754, 1005
AgsI TTSAA 6 cut(s) 101, 287, 433, 506, 641, 833
AhlI ACTAGT 1 cut(s) 280
AjuI GAANNNNNNNTTGG 2 cut(s) 111, 143
AluBI AGCT 8 cut(s) 74, 170, 218, 419, 644, 989, 1003, 1021
AluI AGCT 8 cut(s) 74, 170, 218, 419, 644, 989, 1003, 1021
Alw21I GWGCWC 1 cut(s) 172
Alw26I GTCTC 4 cut(s) 258, 269, 465, 937
AlwI GGATC 6 cut(s) 137, 182, 338, 392, 405, 905
Ama87I CYCGRG 1 cut(s) 165
Aor13HI TCCGGA 1 cut(s) 109
AoxI GGCC 3 cut(s) 150, 440, 457
ApeKI GCWGC 2 cut(s) 74, 839
ApoI RAATTY 1 cut(s) 235
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspS9I GGNCC 2 cut(s) 151, 377
AsuC2I CCSGG 3 cut(s) 29, 79, 720
AsuHPI GGTGA 1 cut(s) 1021
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 1 cut(s) 377
AxyI CCTNAGG 1 cut(s) 402
BalI TGGCCA 1 cut(s) 442
BamHI GGATCC 1 cut(s) 397
BanII GRGCYC 2 cut(s) 172, 526
BbsI GAAGAC 2 cut(s) 429, 680
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 2 cut(s) 61, 851
BccI CCATC 4 cut(s) 293, 304, 388, 964
BceAI ACGGC 3 cut(s) 620, 639, 641
BciVI GTATCC 1 cut(s) 297
BcnI CCSGG 3 cut(s) 29, 79, 720
BcoDI GTCTC 4 cut(s) 258, 269, 465, 937
BcuI ACTAGT 1 cut(s) 280
BfaI CTAG 4 cut(s) 281, 785, 990, 1024
BfuI GTATCC 1 cut(s) 297
BglII AGATCT 1 cut(s) 565
BisI GCNGC 3 cut(s) 75, 460, 840
BlsI GCNGC 3 cut(s) 76, 461, 841
Bme1390I CCNGG 3 cut(s) 29, 79, 720
Bme18I GGWCC 1 cut(s) 377
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 2 cut(s) 151, 377
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 3 cut(s) 29, 79, 720
BmsI GCATC 3 cut(s) 115, 321, 471
BpiI GAAGAC 2 cut(s) 429, 680
BpmI CTGGAG 1 cut(s) 701
Bpu10I CCTNAGC 1 cut(s) 1004
BpuEI CTTGAG 2 cut(s) 176, 940
BpuMI CCSGG 3 cut(s) 29, 79, 720
BsaBI GATNNNNATC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 937
BsaWI WCCGGW 2 cut(s) 45, 109
Bsc4I CCNNNNNNNGG 6 cut(s) 160, 353, 532, 718, 754, 1005
Bse1I ACTGG 2 cut(s) 497, 556
Bse21I CCTNAGG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 141
BseAI TCCGGA 1 cut(s) 109
BseGI GGATG 5 cut(s) 25, 106, 277, 399, 462
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 6 cut(s) 160, 353, 532, 718, 754, 1005
BseMII CTCAG 3 cut(s) 416, 581, 638
BseNI ACTGG 2 cut(s) 497, 556
BseXI GCAGC 2 cut(s) 61, 851
BsgI GTGCAG 1 cut(s) 46
BshFI GGCC 3 cut(s) 152, 442, 459
BsiHKAI GWGCWC 1 cut(s) 172
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 5 cut(s) 29, 46, 78, 110, 719
BslI CCNNNNNNNGG 6 cut(s) 160, 353, 532, 718, 754, 1005
BsmAI GTCTC 4 cut(s) 258, 269, 465, 937
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 3 cut(s) 152, 442, 459
Bso31I GGTCTC 1 cut(s) 937
BsoBI CYCGRG 1 cut(s) 165
Bsp1286I GDGCHC 2 cut(s) 172, 526
Bsp13I TCCGGA 1 cut(s) 109
Bsp143I GATC 8 cut(s) 142, 174, 343, 397, 527, 565, 864, 897
BspACI CCGC 3 cut(s) 460, 587, 842
BspANI GGCC 3 cut(s) 152, 442, 459
BspCNI CTCAG 3 cut(s) 415, 582, 639
BspEI TCCGGA 1 cut(s) 109
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 1 cut(s) 399
BspPI GGATC 6 cut(s) 137, 182, 338, 392, 405, 905
BspTNI GGTCTC 1 cut(s) 937
BsrI ACTGG 2 cut(s) 497, 556
BssMI GATC 8 cut(s) 142, 174, 343, 397, 527, 565, 864, 897
BssNI GRCGYC 1 cut(s) 32
Bst4CI ACNGT 3 cut(s) 67, 752, 910
Bst6I CTCTTC 2 cut(s) 507, 963
BstACI GRCGYC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 369
BstDEI CTNAG 5 cut(s) 402, 590, 647, 939, 1004
BstENI CCTNNNNNAGG 2 cut(s) 158, 351
BstF5I GGATG 5 cut(s) 25, 106, 277, 399, 462
BstKTI GATC 8 cut(s) 145, 177, 346, 400, 530, 568, 867, 900
BstMAI GTCTC 4 cut(s) 258, 269, 465, 937
BstMBI GATC 8 cut(s) 142, 174, 343, 397, 527, 565, 864, 897
BstMWI GCNNNNNNNGC 2 cut(s) 981, 995
BstSCI CCNGG 3 cut(s) 27, 77, 718
BstV1I GCAGC 2 cut(s) 61, 851
BstV2I GAAGAC 2 cut(s) 429, 680
BstX2I RGATCY 2 cut(s) 397, 565
BstYI RGATCY 2 cut(s) 397, 565
Bsu36I CCTNAGG 1 cut(s) 402
BsuI GTATCC 1 cut(s) 297
BsuRI GGCC 3 cut(s) 152, 442, 459
BtsCI GGATG 5 cut(s) 25, 106, 277, 399, 462
BtsIMutI CAGTG 3 cut(s) 63, 367, 549
Cac8I GCNNGC 1 cut(s) 369
CciI TCATGA 1 cut(s) 867
Cfr13I GGNCC 2 cut(s) 151, 377
CseI GACGC 1 cut(s) 21
Csp6I GTAC 2 cut(s) 49, 911
CviAII CATG 4 cut(s) 191, 826, 868, 894
CviQI GTAC 2 cut(s) 49, 911
DdeI CTNAG 5 cut(s) 402, 590, 647, 939, 1004
DpnI GATC 8 cut(s) 144, 176, 345, 399, 529, 567, 866, 899
DpnII GATC 8 cut(s) 142, 174, 343, 397, 527, 565, 864, 897
DraIII CACNNNGTG 2 cut(s) 536, 722
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 1 cut(s) 440
Eam1104I CTCTTC 2 cut(s) 507, 963
EarI CTCTTC 2 cut(s) 507, 963
Ecl136II GAGCTC 1 cut(s) 170
Eco24I GRGCYC 2 cut(s) 172, 526
Eco31I GGTCTC 1 cut(s) 937
Eco47I GGWCC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 170
Eco57I CTGAAG 2 cut(s) 441, 692
Eco81I CCTNAGG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 170
EcoNI CCTNNNNNAGG 2 cut(s) 158, 351
EcoT38I GRGCYC 2 cut(s) 172, 526
FaeI CATG 4 cut(s) 194, 829, 871, 897
FatI CATG 4 cut(s) 190, 825, 867, 893
FauI CCCGC 1 cut(s) 835
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 3 cut(s) 75, 460, 840
FokI GGATG 5 cut(s) 32, 93, 284, 406, 449
FriOI GRGCYC 2 cut(s) 172, 526
Fsp4HI GCNGC 3 cut(s) 75, 460, 840
FspBI CTAG 4 cut(s) 281, 785, 990, 1024
GluI GCNGC 3 cut(s) 75, 460, 840
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 3 cut(s) 152, 442, 459
HapII CCGG 5 cut(s) 29, 46, 78, 110, 719
HgaI GACGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 194, 829, 871, 897
HincII GTYRAC 3 cut(s) 58, 817, 823
HindII GTYRAC 3 cut(s) 58, 817, 823
HindIII AAGCTT 2 cut(s) 216, 1001
HinfI GANTC 5 cut(s) 199, 592, 676, 686, 1012
HpaII CCGG 5 cut(s) 29, 46, 78, 110, 719
HphI GGTGA 1 cut(s) 1021
Hpy166II GTNNAC 4 cut(s) 58, 661, 817, 823
Hpy188I TCNGA 7 cut(s) 71, 174, 242, 414, 648, 961, 1017
Hpy188III TCNNGA 7 cut(s) 17, 110, 383, 596, 680, 785, 868
Hpy8I GTNNAC 4 cut(s) 58, 661, 817, 823
Hpy99I CGWCG 2 cut(s) 59, 331
HpyAV CCTTC 4 cut(s) 95, 154, 213, 593
HpyCH4III ACNGT 3 cut(s) 67, 752, 910
HpyCH4IV ACGT 3 cut(s) 779, 798, 819
HpyCH4V TGCA 6 cut(s) 63, 190, 334, 544, 581, 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 981, 995
HpyF3I CTNAG 5 cut(s) 402, 590, 647, 939, 1004
HpySE526I ACGT 3 cut(s) 779, 798, 819
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 194, 829, 871, 897
Kpn2I TCCGGA 1 cut(s) 109
Kzo9I GATC 8 cut(s) 142, 174, 343, 397, 527, 565, 864, 897
LmnI GCTCC 1 cut(s) 71
Lsp1109I GCAGC 2 cut(s) 61, 851
LweI GCATC 3 cut(s) 115, 321, 471
MaeI CTAG 4 cut(s) 281, 785, 990, 1024
MaeII ACGT 3 cut(s) 779, 798, 819
MalI GATC 8 cut(s) 144, 176, 345, 399, 529, 567, 866, 899
MboI GATC 8 cut(s) 142, 174, 343, 397, 527, 565, 864, 897
MboII GAAGA 7 cut(s) 434, 494, 494, 685, 845, 859, 980
MflI RGATCY 2 cut(s) 397, 565
MhlI GDGCHC 2 cut(s) 172, 526
MlsI TGGCCA 1 cut(s) 442
MluCI AATT 7 cut(s) 96, 178, 235, 513, 765, 930, 948
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 2 cut(s) 601, 670
MmeI TCCRAC 2 cut(s) 49, 593
Mox20I TGGCCA 1 cut(s) 442
MroI TCCGGA 1 cut(s) 109
MscI TGGCCA 1 cut(s) 442
MseI TTAA 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 2 cut(s) 589, 842
MspI CCGG 5 cut(s) 29, 46, 78, 110, 719
MspR9I CCNGG 3 cut(s) 29, 79, 720
Mva1269I GAATGC 1 cut(s) 82
MwoI GCNNNNNNNGC 2 cut(s) 981, 995
NciI CCSGG 3 cut(s) 29, 79, 720
NdeII GATC 8 cut(s) 142, 174, 343, 397, 527, 565, 864, 897
NlaIII CATG 4 cut(s) 194, 829, 871, 897
NlaIV GGNNCC 1 cut(s) 399
NmeAIII GCCGAG 1 cut(s) 1010
PaeR7I CTCGAG 1 cut(s) 165
PagI TCATGA 1 cut(s) 867
PctI GAATGC 1 cut(s) 82
PfeI GAWTC 3 cut(s) 199, 686, 1012
PflMI CCANNNNNTGG 1 cut(s) 754
PkrI GCNGC 3 cut(s) 76, 461, 841
PleI GAGTC 2 cut(s) 600, 670
PpsI GAGTC 2 cut(s) 600, 670
PsiI TTATAA 1 cut(s) 572
Psp124BI GAGCTC 1 cut(s) 172
Psp1406I AACGTT 1 cut(s) 779
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 2 cut(s) 151, 377
PspXI VCTCGAGB 1 cut(s) 165
PsuI RGATCY 2 cut(s) 397, 565
RsaI GTAC 2 cut(s) 50, 912
RsaNI GTAC 2 cut(s) 49, 911
SacI GAGCTC 1 cut(s) 172
SalI GTCGAC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 3 cut(s) 75, 460, 840
Sau3AI GATC 8 cut(s) 142, 174, 343, 397, 527, 565, 864, 897
Sau96I GGNCC 2 cut(s) 151, 377
SchI GAGTC 2 cut(s) 601, 670
ScrFI CCNGG 3 cut(s) 29, 79, 720
SduI GDGCHC 2 cut(s) 172, 526
SfaNI GCATC 3 cut(s) 115, 321, 471
Sfr274I CTCGAG 1 cut(s) 165
SinI GGWCC 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 165
SmlI CTYRAG 3 cut(s) 155, 165, 919
SmoI CTYRAG 3 cut(s) 155, 165, 919
SpeI ACTAGT 1 cut(s) 280
Sse9I AATT 7 cut(s) 96, 178, 235, 513, 765, 930, 948
SsiI CCGC 3 cut(s) 460, 587, 842
SspMI CTAG 4 cut(s) 281, 785, 990, 1024
SstI GAGCTC 1 cut(s) 172
StyD4I CCNGG 3 cut(s) 27, 77, 718
TaaI ACNGT 3 cut(s) 67, 752, 910
TaiI ACGT 3 cut(s) 782, 801, 822
TaqI TCGA 5 cut(s) 57, 141, 166, 329, 595
TasI AATT 7 cut(s) 96, 178, 235, 513, 765, 930, 948
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 3 cut(s) 199, 686, 1012
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 3 cut(s) 70, 367, 556
TseI GCWGC 2 cut(s) 74, 839
TspDTI ATGAA 2 cut(s) 681, 897
TspGWI ACGGA 2 cut(s) 199, 980
TspRI CASTG 3 cut(s) 70, 367, 556
Van91I CCANNNNNTGG 1 cut(s) 754
VpaK11BI GGWCC 1 cut(s) 377
XagI CCTNNNNNAGG 2 cut(s) 158, 351
XapI RAATTY 1 cut(s) 235
XbaI TCTAGA 1 cut(s) 784
XhoI CTCGAG 1 cut(s) 165
XmiI GTMKAC 1 cut(s) 57
XspI CTAG 4 cut(s) 281, 785, 990, 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.