AT5G12110

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011718
Physical Location & Seq
Forward (+)
3914468 .. 3916896
2429 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12110.1

Sequence Viewer

Length: 687 bp
ATGGCGGTTACATTTTCTGATCTCCACACAGAGCGAGGTCTCAAAACCCTCGAGGAACATCTCGCCGGTAAAACGTACATCTCCGGCGATCAGCTTTCGGTTGATGATGTGAAGGTTTACGCTGCCGTATTGGAGAATCCAGGTGATGGTTTTCCCAATGCTAGCAAGTGGTACGATTCTGTTGCTTCTCACCTAGCTAAAAGTTTTCCCGGGAAAGCAGACGGAGTGAGAGTTGGTGGTGGTGTTGCTCCTCCATCTGAAGCTCATCCACATACTGAGGAACCTGCTGCTGATGGTGATGGTGATGATGATGATGATATTGATCTTTTCGCTGATGAGACTGAAGATGAGAAAAAAGCTGCTGAAGAGAGAGAAGCTGCTAAGAAAGATACTAAGAAGACTAAAGAGAGTGGAAAATCTTCTGTGCTACTGGAAGTAAAACCATGGGATGATGAAACCGACATGAAGAAGTTAGAAGAAGCTGTTCGTAGTGTTCAGATGCCAGGTCTTACATGGGGAGCTTCGAAATTGGTACCTGTTGGTTACGGGATAAAGAAACTCACAATCATGATGACAATTGTTGATGACCTTGTGTCTGTGGACAACCTCATTGAAGACCATCTCACCTCAGAGCCTAACAACGAGTACATCCAAAGTGTCGATATTGTCGCTTTTAACAAGATTTAG

Protein Analysis

228

Amino Acids

24.79

Weight (kDa)

4.53

Isoelectric Point (pI)

29.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 141 - 228 8.9e-32 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011718)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12110 AT5G19510
fragaria_vesca FvH4_4g13950
malus_domestica MD02G1099500.v1.1 MD02G1271700.v1.1 MD07G1040800.v1.1
prunus_persica Prupe.5G005100_v2.0.a1
pyrus_communis pycom02g23230 pycom07g02830
rosa_chinensis RchiOBHm_Chr4g0419721
rosa_laevigata RLG00000007801
rosa_multiflora Rmu_sc0016868.1_g000008
rosa_roxburghii Rroxscaffold_5G00362560
rosa_rugosa Rorug04G0160000
rosa_samantha Rh4AG221200 Rh4BG223200 Rh4CG234500 Rh4DG220200
rosa_wichuraiana Rw4G019040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 292
Acc65I GGTACC 1 cut(s) 532
AccB1I GGYRCC 1 cut(s) 532
AccB7I CCANNNNNTGG 1 cut(s) 146
AciI CCGC 1 cut(s) 5
AcuI CTGAAG 3 cut(s) 279, 363, 384
AfaI GTAC 4 cut(s) 77, 173, 534, 647
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 1 cut(s) 614
AhdI GACNNNNNGTC 1 cut(s) 592
AjnI CCWGG 2 cut(s) 139, 502
AluBI AGCT 7 cut(s) 94, 197, 263, 359, 377, 482, 521
AluI AGCT 7 cut(s) 94, 197, 263, 359, 377, 482, 521
Alw26I GTCTC 2 cut(s) 44, 332
Ama87I CYCGRG 2 cut(s) 50, 209
ApeKI GCWGC 4 cut(s) 122, 287, 359, 377
Asp700I GAANNNNTTC 2 cut(s) 418, 483
Asp718I GGTACC 1 cut(s) 532
AsuC2I CCSGG 2 cut(s) 210, 211
AsuHPI GGTGA 5 cut(s) 155, 182, 308, 314, 616
AsuII TTCGAA 1 cut(s) 524
AsuNHI GCTAGC 1 cut(s) 161
AvaI CYCGRG 2 cut(s) 50, 209
BanI GGYRCC 1 cut(s) 532
BbsI GAAGAC 2 cut(s) 404, 621
BbvI GCAGC 4 cut(s) 109, 274, 346, 364
BccI CCATC 5 cut(s) 140, 262, 287, 293, 627
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 2 cut(s) 141, 504
BcnI CCSGG 2 cut(s) 210, 211
BcoDI GTCTC 2 cut(s) 44, 332
BfaI CTAG 2 cut(s) 162, 194
BfuAI ACCTGC 1 cut(s) 292
BisI GCNGC 4 cut(s) 123, 288, 360, 378
BlsI GCNGC 4 cut(s) 124, 289, 361, 379
Bme1390I CCNGG 4 cut(s) 141, 210, 211, 504
BmeRI GACNNNNNGTC 1 cut(s) 592
BmeT110I CYCGRG 2 cut(s) 50, 209
BmiI GGNNCC 2 cut(s) 282, 534
BmrFI CCNGG 4 cut(s) 141, 210, 211, 504
BmsI GCATC 1 cut(s) 489
BmtI GCTAGC 1 cut(s) 165
BpiI GAAGAC 2 cut(s) 404, 621
Bpu14I TTCGAA 1 cut(s) 524
BpuMI CCSGG 2 cut(s) 210, 211
BsaBI GATNNNNATC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 209, 443
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 435
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 2 cut(s) 141, 504
BseDI CCNNGG 2 cut(s) 209, 443
BseGI GGATG 3 cut(s) 265, 454, 648
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 2 cut(s) 267, 642
BseNI ACTGG 1 cut(s) 435
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 4 cut(s) 109, 274, 346, 364
BshNI GGYRCC 1 cut(s) 532
BsiHKCI CYCGRG 2 cut(s) 50, 209
BsiSI CCGG 3 cut(s) 66, 84, 210
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 2 cut(s) 44, 332
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 2 cut(s) 50, 209
Bsp119I TTCGAA 1 cut(s) 524
Bsp143I GATC 3 cut(s) 19, 88, 322
Bsp19I CCATGG 1 cut(s) 443
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 268, 641
BspHI TCATGA 1 cut(s) 567
BspLI GGNNCC 2 cut(s) 282, 534
BspMI ACCTGC 1 cut(s) 292
BspOI GCTAGC 1 cut(s) 165
BspT104I TTCGAA 1 cut(s) 524
BspT107I GGYRCC 1 cut(s) 532
BspTNI GGTCTC 1 cut(s) 44
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 209, 443
BssMI GATC 3 cut(s) 19, 88, 322
BssT1I CCWWGG 1 cut(s) 443
Bst2UI CCWGG 2 cut(s) 141, 504
Bst6I CTCTTC 1 cut(s) 360
BstBI TTCGAA 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 4 cut(s) 276, 381, 393, 628
BstDSI CCRYGG 1 cut(s) 443
BstF5I GGATG 3 cut(s) 265, 454, 648
BstKTI GATC 3 cut(s) 22, 91, 325
BstMAI GTCTC 2 cut(s) 44, 332
BstMBI GATC 3 cut(s) 19, 88, 322
BstNI CCWGG 2 cut(s) 141, 504
BstSCI CCNGG 4 cut(s) 139, 208, 209, 502
BstV1I GCAGC 4 cut(s) 109, 274, 346, 364
BstV2I GAAGAC 2 cut(s) 404, 621
BtgI CCRYGG 1 cut(s) 443
BtsCI GGATG 3 cut(s) 265, 454, 648
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 163
CciI TCATGA 1 cut(s) 567
Cfr10I RCCGGY 1 cut(s) 65
Cfr9I CCCGGG 1 cut(s) 209
Csp6I GTAC 4 cut(s) 76, 172, 533, 646
CviAII CATG 4 cut(s) 444, 463, 513, 568
CviJI RGCY 8 cut(s) 94, 197, 263, 359, 377, 482, 521, 634
CviKI_1 RGCY 8 cut(s) 94, 197, 263, 359, 377, 482, 521, 634
CviQI GTAC 4 cut(s) 76, 172, 533, 646
DdeI CTNAG 4 cut(s) 276, 381, 393, 628
DpnI GATC 3 cut(s) 21, 90, 324
DpnII GATC 3 cut(s) 19, 88, 322
DriI GACNNNNNGTC 1 cut(s) 592
Eam1104I CTCTTC 1 cut(s) 360
Eam1105I GACNNNNNGTC 1 cut(s) 592
EarI CTCTTC 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 443
Eco31I GGTCTC 1 cut(s) 44
Eco57I CTGAAG 3 cut(s) 279, 363, 384
Eco88I CYCGRG 2 cut(s) 50, 209
EcoRII CCWGG 2 cut(s) 139, 502
EcoT14I CCWWGG 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 4 cut(s) 447, 466, 516, 571
FaiI YATR 5 cut(s) 273, 445, 464, 514, 569
FatI CATG 4 cut(s) 443, 462, 512, 567
Fnu4HI GCNGC 4 cut(s) 123, 288, 360, 378
FokI GGATG 3 cut(s) 252, 461, 635
Fsp4HI GCNGC 4 cut(s) 123, 288, 360, 378
FspBI CTAG 2 cut(s) 162, 194
GluI GCNGC 4 cut(s) 123, 288, 360, 378
HapII CCGG 3 cut(s) 66, 84, 210
Hin1II CATG 4 cut(s) 447, 466, 516, 571
HinfI GANTC 2 cut(s) 136, 176
HpaII CCGG 3 cut(s) 66, 84, 210
HphI GGTGA 5 cut(s) 155, 182, 308, 314, 616
Hpy166II GTNNAC 2 cut(s) 118, 601
Hpy188I TCNGA 4 cut(s) 19, 259, 498, 631
Hpy188III TCNNGA 1 cut(s) 568
Hpy8I GTNNAC 2 cut(s) 118, 601
HpyAV CCTTC 1 cut(s) 106
HpyCH4IV ACGT 1 cut(s) 74
HpyF3I CTNAG 4 cut(s) 276, 381, 393, 628
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 4 cut(s) 447, 466, 516, 571
KpnI GGTACC 1 cut(s) 536
Kzo9I GATC 3 cut(s) 19, 88, 322
LmnI GCTCC 2 cut(s) 253, 518
Lsp1109I GCAGC 4 cut(s) 109, 274, 346, 364
LweI GCATC 1 cut(s) 489
MaeI CTAG 2 cut(s) 162, 194
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 2 cut(s) 7, 542
MalI GATC 3 cut(s) 21, 90, 324
MboI GATC 3 cut(s) 19, 88, 322
MboII GAAGA 7 cut(s) 356, 377, 409, 411, 478, 488, 626
MfeI CAATTG 1 cut(s) 576
MluCI AATT 2 cut(s) 527, 576
MnlI CCTC 7 cut(s) 29, 46, 59, 261, 271, 617, 637
MroXI GAANNNNTTC 2 cut(s) 418, 483
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 566
MspI CCGG 3 cut(s) 66, 84, 210
MspR9I CCNGG 4 cut(s) 141, 210, 211, 504
MunI CAATTG 1 cut(s) 576
MvaI CCWGG 2 cut(s) 141, 504
NciI CCSGG 2 cut(s) 210, 211
NcoI CCATGG 1 cut(s) 443
NdeII GATC 3 cut(s) 19, 88, 322
NheI GCTAGC 1 cut(s) 161
NlaIII CATG 4 cut(s) 447, 466, 516, 571
NlaIV GGNNCC 2 cut(s) 282, 534
NspV TTCGAA 1 cut(s) 524
PaeR7I CTCGAG 1 cut(s) 50
PagI TCATGA 1 cut(s) 567
PdmI GAANNNNTTC 2 cut(s) 418, 483
PfeI GAWTC 2 cut(s) 136, 176
PflMI CCANNNNNTGG 1 cut(s) 146
PkrI GCNGC 4 cut(s) 124, 289, 361, 379
Psp6I CCWGG 2 cut(s) 139, 502
PspGI CCWGG 2 cut(s) 139, 502
PspN4I GGNNCC 2 cut(s) 282, 534
PspXI VCTCGAGB 1 cut(s) 50
RsaI GTAC 4 cut(s) 77, 173, 534, 647
RsaNI GTAC 4 cut(s) 76, 172, 533, 646
RseI CAYNNNNRTG 1 cut(s) 566
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 4 cut(s) 123, 288, 360, 378
Sau3AI GATC 3 cut(s) 19, 88, 322
ScrFI CCNGG 4 cut(s) 141, 210, 211, 504
SfaNI GCATC 1 cut(s) 489
Sfr274I CTCGAG 1 cut(s) 50
SfuI TTCGAA 1 cut(s) 524
SlaI CTCGAG 1 cut(s) 50
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 2 cut(s) 527, 576
SsiI CCGC 1 cut(s) 5
SspMI CTAG 2 cut(s) 162, 194
StyD4I CCNGG 4 cut(s) 139, 208, 209, 502
StyI CCWWGG 1 cut(s) 443
TaiI ACGT 1 cut(s) 77
TaqI TCGA 3 cut(s) 51, 524, 660
TasI AATT 2 cut(s) 527, 576
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 2 cut(s) 136, 176
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseI GCWGC 4 cut(s) 122, 287, 359, 377
TspDTI ATGAA 2 cut(s) 468, 479
TspGWI ACGGA 1 cut(s) 237
TspMI CCCGGG 1 cut(s) 209
Van91I CCANNNNNTGG 1 cut(s) 146
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XhoI CTCGAG 1 cut(s) 50
XmaI CCCGGG 1 cut(s) 209
XmnI GAANNNNTTC 2 cut(s) 418, 483
XspI CTAG 2 cut(s) 162, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.