MD02G1271700.v1.1

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011718
Physical Location & Seq
Forward (+)
32627295 .. 32629493
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1271700.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGCCATCACCTTCTCAGATCTCTACACCGAGGCCGGTCTCAAGTCCCTCGACGAGTTCCTCGCCGGAAAATCTTACATCTCCGGAGACAAGCTGACTTTGGATGATATCAAGGTGTACGCCGCTGTGCTGGAAAAGCCCGCCGGTTCCTTTGCCAATGTGAGCAAGTGGTACGACGGCGTTTCAGCCCAACTCGCTGCAAACTTCCCCGGCAAGGCTGCCGGAGTGAGAGTCAGCAGCGGCAAGGCCGAGTCTGCTGCTCCTGCTCCATCTGCCGCTGCTGGAGGCGATGCCGATGACGATTTGGACCTTTTTGGAGATGAAACCGAGGAGGACAAGAAGGCCGAGGCGGAGATGGCGGCAGCCAAAAAGGCTTCTTTGACAATCGCCATGGCCATCACCTTCTCAGATCTCTACACCGAGGCCGGTCTCAAGTCCCTCGACGAGTTCCTCGCCGGAAAATCTTACATCTCCGGAGACAAGCTGACTTTGGATGATATCAAGGTGTACGCCGCTGTGCTGGAAAAGCCCGCCGGTTCCGTTGCCAATGTGAGCAAGTGGTACGACGGCGTTTCAGCCCAACTCGCTGCAAACTTCCCCGGCAAGGCTGCCGGAGTGAGAGTCAGCAGCGGCAAGGCCGAGTCTGCTGCTCCTGCTCCATCTGCCGCTGCTGGAGGCGATGCCGATGACGATTTGGACCTTTTTGGAGATGAAACCGAGGAGGACAAGAAGGCCGAGGCGGAGATGGCGGCAGCCAAAAAGGCTTCTACCAAGAAGAAGGAGAGTGGGAAATCCTCTGTTCTTTTGGATGTCAAGCCTTGGGATGATGAGACTGACATGAAGAAGCTCGAGGAGGCTGTTCGGAGTGTCGAGAAGGAGGGTCTCTTCTGGGGCGCATCGAAATTGGTTGCTGTTGGTTACGGGATCAAGAAGTTGCAGATCATGCTCACCATTGTCGATGACCTTGTTTCCGTCGATGACCTCATCGAAGAGCAGCTTACCGCCGAGCCCCGCAATGAGTATATTCAGAGCTGTGACATTGTTGCCTTCAACAAAATTTAA

Protein Analysis

354

Amino Acids

37.13

Weight (kDa)

4.48

Isoelectric Point (pI)

28.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 266 - 353 5.8e-30 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011718)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12110 AT5G19510
fragaria_vesca FvH4_4g13950
malus_domestica MD02G1099500.v1.1 MD02G1271700.v1.1 MD07G1040800.v1.1
prunus_persica Prupe.5G005100_v2.0.a1
pyrus_communis pycom02g23230 pycom07g02830
rosa_chinensis RchiOBHm_Chr4g0419721
rosa_laevigata RLG00000007801
rosa_multiflora Rmu_sc0016868.1_g000008
rosa_roxburghii Rroxscaffold_5G00362560
rosa_rugosa Rorug04G0160000
rosa_samantha Rh4AG221200 Rh4BG223200 Rh4CG234500 Rh4DG220200
rosa_wichuraiana Rw4G019040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 83, 473
AclWI GGATC 1 cut(s) 932
AcoI YGGCCR 2 cut(s) 3, 393
AcsI RAATTY 1 cut(s) 1056
AfaI GTAC 4 cut(s) 119, 173, 509, 563
AfiI CCNNNNNNNGG 2 cut(s) 214, 604
AgsI TTSAA 1 cut(s) 1051
AluBI AGCT 5 cut(s) 94, 484, 847, 997, 1032
AluI AGCT 5 cut(s) 94, 484, 847, 997, 1032
Alw26I GTCTC 6 cut(s) 44, 81, 434, 471, 824, 887
AlwI GGATC 1 cut(s) 932
Ama87I CYCGRG 1 cut(s) 848
Aor13HI TCCGGA 2 cut(s) 83, 473
AoxI GGCC 8 cut(s) 3, 33, 246, 342, 393, 423, 636, 732
ApoI RAATTY 1 cut(s) 1056
AspLEI GCGC 1 cut(s) 896
AspS9I GGNCC 2 cut(s) 307, 697
AsuC2I CCSGG 2 cut(s) 210, 600
AsuHPI GGTGA 2 cut(s) 391, 940
AvaI CYCGRG 1 cut(s) 848
AvaII GGWCC 2 cut(s) 307, 697
BalI TGGCCA 2 cut(s) 5, 395
BanII GRGCYC 1 cut(s) 1011
BccI CCATC 6 cut(s) 14, 277, 349, 404, 667, 739
BceAI ACGGC 2 cut(s) 193, 583
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 629, 663
BcnI CCSGG 2 cut(s) 210, 600
BcoDI GTCTC 6 cut(s) 44, 81, 434, 471, 824, 887
BglI GCCNNNNNGGC 2 cut(s) 371, 761
BglII AGATCT 2 cut(s) 19, 409
Bme1390I CCNGG 2 cut(s) 210, 600
Bme18I GGWCC 2 cut(s) 307, 697
BmeT110I CYCGRG 1 cut(s) 848
BmgT120I GGNCC 2 cut(s) 307, 697
BmiI GGNNCC 2 cut(s) 148, 538
BmrFI CCNGG 2 cut(s) 210, 600
BmsI GCATC 3 cut(s) 280, 670, 905
BpmI CTGGAG 2 cut(s) 303, 693
BpuEI CTTGAG 2 cut(s) 26, 416
BpuMI CCSGG 2 cut(s) 210, 600
BsaI GGTCTC 3 cut(s) 44, 434, 887
BsaWI WCCGGW 2 cut(s) 83, 473
BsaXI ACNNNNNCTCC 4 cut(s) 216, 246, 606, 636
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 604
Bse118I RCCGGY 4 cut(s) 35, 143, 425, 533
Bse3DI GCAATG 1 cut(s) 1021
BseAI TCCGGA 2 cut(s) 83, 473
BseGI GGATG 4 cut(s) 109, 499, 814, 829
BseLI CCNNNNNNNGG 2 cut(s) 214, 604
BseMI GCAATG 1 cut(s) 1021
BseMII CTCAG 2 cut(s) 30, 420
BseRI GAGGAG 3 cut(s) 344, 734, 866
BshFI GGCC 8 cut(s) 5, 35, 248, 344, 395, 425, 638, 734
BsiHKCI CYCGRG 1 cut(s) 848
BslFI GGGAC 2 cut(s) 31, 421
BslI CCNNNNNNNGG 2 cut(s) 214, 604
BsmAI GTCTC 6 cut(s) 44, 81, 434, 471, 824, 887
BsmFI GGGAC 2 cut(s) 31, 421
BsnI GGCC 8 cut(s) 5, 35, 248, 344, 395, 425, 638, 734
Bso31I GGTCTC 3 cut(s) 44, 434, 887
BsoBI CYCGRG 1 cut(s) 848
Bsp1286I GDGCHC 1 cut(s) 1011
Bsp13I TCCGGA 2 cut(s) 83, 473
Bsp143I GATC 4 cut(s) 19, 409, 924, 939
Bsp19I CCATGG 1 cut(s) 390
BspANI GGCC 8 cut(s) 5, 35, 248, 344, 395, 425, 638, 734
BspCNI CTCAG 2 cut(s) 29, 419
BspEI TCCGGA 2 cut(s) 83, 473
BspLI GGNNCC 2 cut(s) 148, 538
BspPI GGATC 1 cut(s) 932
BspQI GCTCTTC 1 cut(s) 984
BspTNI GGTCTC 3 cut(s) 44, 434, 887
BsrDI GCAATG 1 cut(s) 1021
BsrFI RCCGGY 4 cut(s) 35, 143, 425, 533
BssAI RCCGGY 4 cut(s) 35, 143, 425, 533
BssMI GATC 4 cut(s) 19, 409, 924, 939
BssT1I CCWWGG 2 cut(s) 390, 818
Bst6I CTCTTC 2 cut(s) 890, 984
BstAPI GCANNNNNTGC 1 cut(s) 943
BstC8I GCNNGC 2 cut(s) 141, 531
BstDEI CTNAG 2 cut(s) 16, 406
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 4 cut(s) 109, 499, 814, 829
BstHHI GCGC 1 cut(s) 896
BstKTI GATC 4 cut(s) 22, 412, 927, 942
BstMAI GTCTC 6 cut(s) 44, 81, 434, 471, 824, 887
BstMBI GATC 4 cut(s) 19, 409, 924, 939
BstSCI CCNGG 2 cut(s) 208, 598
BstX2I RGATCY 2 cut(s) 19, 409
BstYI RGATCY 2 cut(s) 19, 409
BsuRI GGCC 8 cut(s) 5, 35, 248, 344, 395, 425, 638, 734
BtgI CCRYGG 1 cut(s) 390
BtgZI GCGATG 2 cut(s) 303, 693
BtsCI GGATG 4 cut(s) 109, 499, 814, 829
Cac8I GCNNGC 2 cut(s) 141, 531
CfoI GCGC 1 cut(s) 896
Cfr10I RCCGGY 4 cut(s) 35, 143, 425, 533
Cfr13I GGNCC 2 cut(s) 307, 697
Csp6I GTAC 4 cut(s) 118, 172, 508, 562
CviAII CATG 3 cut(s) 391, 838, 943
CviQI GTAC 4 cut(s) 118, 172, 508, 562
DdeI CTNAG 2 cut(s) 16, 406
DpnI GATC 4 cut(s) 21, 411, 926, 941
DpnII GATC 4 cut(s) 19, 409, 924, 939
EaeI YGGCCR 2 cut(s) 3, 393
Eam1104I CTCTTC 2 cut(s) 890, 984
EarI CTCTTC 2 cut(s) 890, 984
EciI GGCGGA 2 cut(s) 365, 755
Eco130I CCWWGG 2 cut(s) 390, 818
Eco24I GRGCYC 1 cut(s) 1011
Eco31I GGTCTC 3 cut(s) 44, 434, 887
Eco32I GATATC 2 cut(s) 109, 499
Eco47I GGWCC 2 cut(s) 307, 697
Eco88I CYCGRG 1 cut(s) 848
EcoRV GATATC 2 cut(s) 109, 499
EcoT14I CCWWGG 2 cut(s) 390, 818
EcoT38I GRGCYC 1 cut(s) 1011
ErhI CCWWGG 2 cut(s) 390, 818
FaeI CATG 3 cut(s) 394, 841, 946
FaiI YATR 4 cut(s) 392, 839, 944, 1023
FalI AAGNNNNNCTT 2 cut(s) 981, 1013
FaqI GGGAC 2 cut(s) 31, 421
FatI CATG 3 cut(s) 390, 837, 942
FauI CCCGC 3 cut(s) 148, 538, 1019
FokI GGATG 4 cut(s) 116, 506, 821, 836
FriOI GRGCYC 1 cut(s) 1011
GlaI GCGC 1 cut(s) 895
GsuI CTGGAG 2 cut(s) 303, 693
HaeIII GGCC 8 cut(s) 5, 35, 248, 344, 395, 425, 638, 734
HhaI GCGC 1 cut(s) 896
Hin1II CATG 3 cut(s) 394, 841, 946
Hin6I GCGC 1 cut(s) 894
HinP1I GCGC 1 cut(s) 894
HinfI GANTC 4 cut(s) 231, 251, 621, 641
HphI GGTGA 2 cut(s) 391, 940
Hpy166II GTNNAC 2 cut(s) 118, 508
Hpy188I TCNGA 4 cut(s) 19, 409, 864, 1029
Hpy188III TCNNGA 4 cut(s) 84, 474, 871, 928
Hpy8I GTNNAC 2 cut(s) 118, 508
Hpy99I CGWCG 5 cut(s) 56, 179, 446, 569, 977
HpyAV CCTTC 7 cut(s) 22, 334, 412, 724, 772, 868, 1057
HpyCH4V TGCA 3 cut(s) 200, 590, 937
HpyF3I CTNAG 2 cut(s) 16, 406
Hsp92II CATG 3 cut(s) 394, 841, 946
HspAI GCGC 1 cut(s) 894
Kpn2I TCCGGA 2 cut(s) 83, 473
Kzo9I GATC 4 cut(s) 19, 409, 924, 939
LguI GCTCTTC 1 cut(s) 984
LmnI GCTCC 4 cut(s) 265, 271, 655, 661
LweI GCATC 3 cut(s) 280, 670, 905
MaeIII GTNAC 2 cut(s) 917, 1034
MalI GATC 4 cut(s) 21, 411, 926, 941
MboI GATC 4 cut(s) 19, 409, 924, 939
MboII GAAGA 4 cut(s) 787, 853, 877, 1001
MflI RGATCY 2 cut(s) 19, 409
MhlI GDGCHC 1 cut(s) 1011
MlsI TGGCCA 2 cut(s) 5, 395
MluCI AATT 2 cut(s) 902, 1056
MluNI TGGCCA 2 cut(s) 5, 395
MlyI GAGTC 4 cut(s) 240, 260, 630, 650
Mox20I TGGCCA 2 cut(s) 5, 395
MroI TCCGGA 2 cut(s) 83, 473
MscI TGGCCA 2 cut(s) 5, 395
MseI TTAA 1 cut(s) 1060
Msp20I TGGCCA 2 cut(s) 5, 395
MspA1I CMGCKG 6 cut(s) 125, 240, 278, 515, 630, 668
MspR9I CCNGG 2 cut(s) 210, 600
NciI CCSGG 2 cut(s) 210, 600
NcoI CCATGG 1 cut(s) 390
NdeII GATC 4 cut(s) 19, 409, 924, 939
NlaIII CATG 3 cut(s) 394, 841, 946
NlaIV GGNNCC 2 cut(s) 148, 538
NmeAIII GCCGAG 5 cut(s) 274, 370, 664, 760, 1030
NmuCI GTSAC 1 cut(s) 1034
PaeR7I CTCGAG 1 cut(s) 848
PciSI GCTCTTC 1 cut(s) 984
PleI GAGTC 4 cut(s) 239, 259, 629, 649
PpsI GAGTC 4 cut(s) 239, 259, 629, 649
PspN4I GGNNCC 2 cut(s) 148, 538
PspPI GGNCC 2 cut(s) 307, 697
PspXI VCTCGAGB 1 cut(s) 848
PsuI RGATCY 2 cut(s) 19, 409
RsaI GTAC 4 cut(s) 119, 173, 509, 563
RsaNI GTAC 4 cut(s) 118, 172, 508, 562
SapI GCTCTTC 1 cut(s) 984
SaqAI TTAA 1 cut(s) 1060
Sau3AI GATC 4 cut(s) 19, 409, 924, 939
Sau96I GGNCC 2 cut(s) 307, 697
SchI GAGTC 4 cut(s) 240, 260, 630, 650
ScrFI CCNGG 2 cut(s) 210, 600
SduI GDGCHC 1 cut(s) 1011
SfaNI GCATC 3 cut(s) 280, 670, 905
Sfr274I CTCGAG 1 cut(s) 848
SinI GGWCC 2 cut(s) 307, 697
SlaI CTCGAG 1 cut(s) 848
SmlI CTYRAG 3 cut(s) 41, 431, 848
SmoI CTYRAG 3 cut(s) 41, 431, 848
Sse9I AATT 2 cut(s) 902, 1056
StyD4I CCNGG 2 cut(s) 208, 598
StyI CCWWGG 2 cut(s) 390, 818
TaqI TCGA 8 cut(s) 51, 441, 849, 870, 899, 957, 975, 987
TasI AATT 2 cut(s) 902, 1056
TauI GCSGC 8 cut(s) 125, 243, 278, 362, 515, 633, 668, 752
Tru1I TTAA 1 cut(s) 1060
Tru9I TTAA 1 cut(s) 1060
TseFI GTSAC 1 cut(s) 1034
Tsp45I GTSAC 1 cut(s) 1034
TspDTI ATGAA 3 cut(s) 336, 726, 854
TspGWI ACGGA 2 cut(s) 529, 961
VpaK11BI GGWCC 2 cut(s) 307, 697
XapI RAATTY 1 cut(s) 1056
XhoI CTCGAG 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.