Rh4DG220200

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011718
Physical Location & Seq
Reverse (-)
42170415 .. 42173022
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG220200.1

Sequence Viewer

Length: 498 bp
ATGAAAATCAGAAGCAAGAGTAATTTCCACGTTTGTTTCAAGATTGCACTGAGGGGTGAATCCTTGAAGCTTTTACTTAGAATGGTTTCTTTTGTACCTGTTCAGGCTGCAGCAGAAGATGATGATGACCTTGATCTCTTCGGTGATGAAACAGAGGAGGACAAGAAGGCAGCAGAGGAGATTCAGGCAGCTAAGAAGCCTTCCGCCAAGAAGAAAGAGAGTGGAAAATCCTCTGTCCTTCTGGATGTGAAGCCTTGGGATGATGAAACAGACATGAAGAAGCTTGAAGAGGCTGTTAGAAGCATTGAAATGGAGGGTCTCTTCTGGGGTGCATCAAAACTGGTGGCAGTTGGTTATGGGATAAAGAAGCTCCAGATCATGCTCACTATTGTTGATGATCTGGTGTCTGTGGATACTCTCATCGAGGAGCGTCTTACTGTTGAGCCATGTGATGAGTGGATCCAGAGCTGTGACATTGTTGCCTTCAACAAGATTTAA

Protein Analysis

165

Amino Acids

18.54

Weight (kDa)

4.77

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 78 - 165 4.8e-31 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011718)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12110 AT5G19510
fragaria_vesca FvH4_4g13950
malus_domestica MD02G1099500.v1.1 MD02G1271700.v1.1 MD07G1040800.v1.1
prunus_persica Prupe.5G005100_v2.0.a1
pyrus_communis pycom02g23230 pycom07g02830
rosa_chinensis RchiOBHm_Chr4g0419721
rosa_laevigata RLG00000007801
rosa_multiflora Rmu_sc0016868.1_g000008
rosa_roxburghii Rroxscaffold_5G00362560
rosa_rugosa Rorug04G0160000
rosa_samantha Rh4AG221200 Rh4BG223200 Rh4CG234500 Rh4DG220200
rosa_wichuraiana Rw4G019040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 204
AclWI GGATC 2 cut(s) 454, 467
AfaI GTAC 1 cut(s) 96
AgsI TTSAA 5 cut(s) 40, 67, 287, 308, 487
AluBI AGCT 5 cut(s) 70, 191, 283, 370, 468
AluI AGCT 5 cut(s) 70, 191, 283, 370, 468
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 2 cut(s) 454, 467
ApeKI GCWGC 4 cut(s) 107, 110, 170, 188
Asp700I GAANNNNTTC 1 cut(s) 85
AsuHPI GGTGA 2 cut(s) 68, 155
BamHI GGATCC 1 cut(s) 459
BbvI GCAGC 4 cut(s) 94, 122, 182, 200
BciVI GTATCC 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 108
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 4 cut(s) 108, 111, 171, 189
BlsI GCNGC 4 cut(s) 109, 112, 172, 190
BmiI GGNNCC 1 cut(s) 461
BmsI GCATC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 356
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 254
BseGI GGATG 2 cut(s) 250, 265
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 1 cut(s) 345
BseRI GAGGAG 3 cut(s) 170, 191, 440
BseXI GCAGC 4 cut(s) 94, 122, 182, 200
BsmAI GTCTC 1 cut(s) 323
Bso31I GGTCTC 1 cut(s) 323
Bsp143I GATC 4 cut(s) 133, 375, 397, 459
BspACI CCGC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 461
BspMAI CTGCAG 1 cut(s) 112
BspPI GGATC 2 cut(s) 454, 467
BspTNI GGTCTC 1 cut(s) 323
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 254
BssMI GATC 4 cut(s) 133, 375, 397, 459
BssT1I CCWWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 3 cut(s) 143, 282, 326
BstDEI CTNAG 3 cut(s) 50, 77, 192
BstF5I GGATG 2 cut(s) 250, 265
BstKTI GATC 4 cut(s) 136, 378, 400, 462
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 4 cut(s) 133, 375, 397, 459
BstSFI CTRYAG 1 cut(s) 108
BstV1I GCAGC 4 cut(s) 94, 122, 182, 200
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BsuI GTATCC 1 cut(s) 406
BtsCI GGATG 2 cut(s) 250, 265
BtsIMutI CAGTG 1 cut(s) 47
CseI GACGC 1 cut(s) 419
Csp6I GTAC 1 cut(s) 95
CspCI CAANNNNNGTGG 2 cut(s) 324, 359
CviAII CATG 3 cut(s) 274, 379, 447
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 3 cut(s) 50, 77, 192
DpnI GATC 4 cut(s) 135, 377, 399, 461
DpnII GATC 4 cut(s) 133, 375, 397, 459
Eam1104I CTCTTC 3 cut(s) 143, 282, 326
EarI CTCTTC 3 cut(s) 143, 282, 326
EciI GGCGGA 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 254
Eco31I GGTCTC 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 254
FaeI CATG 3 cut(s) 277, 382, 450
FaiI YATR 4 cut(s) 275, 357, 380, 448
FatI CATG 3 cut(s) 273, 378, 446
Fnu4HI GCNGC 4 cut(s) 108, 111, 171, 189
FokI GGATG 2 cut(s) 257, 272
Fsp4HI GCNGC 4 cut(s) 108, 111, 171, 189
GluI GCNGC 4 cut(s) 108, 111, 171, 189
GsuI CTGGAG 1 cut(s) 356
HgaI GACGC 1 cut(s) 419
Hin1II CATG 3 cut(s) 277, 382, 450
HindIII AAGCTT 2 cut(s) 68, 281
HinfI GANTC 2 cut(s) 59, 181
HphI GGTGA 2 cut(s) 68, 155
Hpy188I TCNGA 1 cut(s) 11
Hpy188III TCNNGA 4 cut(s) 40, 242, 373, 463
HpyAV CCTTC 4 cut(s) 160, 210, 248, 493
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 3 cut(s) 47, 110, 332
HpyF3I CTNAG 3 cut(s) 50, 77, 192
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 3 cut(s) 277, 382, 450
Kzo9I GATC 4 cut(s) 133, 375, 397, 459
LmnI GCTCC 2 cut(s) 375, 427
LpnPI CCDG 9 cut(s) 89, 111, 170, 227, 310, 326, 386, 386, 476
Lsp1109I GCAGC 4 cut(s) 94, 122, 182, 200
LweI GCATC 1 cut(s) 341
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 470
MalI GATC 4 cut(s) 135, 377, 399, 461
MboI GATC 4 cut(s) 133, 375, 397, 459
MboII GAAGA 6 cut(s) 128, 130, 223, 289, 299, 313
MflI RGATCY 1 cut(s) 459
MluCI AATT 1 cut(s) 22
MnlI CCTC 8 cut(s) 45, 148, 151, 169, 241, 283, 307, 418
MroXI GAANNNNTTC 1 cut(s) 85
MseI TTAA 1 cut(s) 496
MslI CAYNNNNRTG 1 cut(s) 308
NdeII GATC 4 cut(s) 133, 375, 397, 459
NlaIII CATG 3 cut(s) 277, 382, 450
NlaIV GGNNCC 1 cut(s) 461
NmuCI GTSAC 1 cut(s) 470
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 2 cut(s) 59, 181
PkrI GCNGC 4 cut(s) 109, 112, 172, 190
PspN4I GGNNCC 1 cut(s) 461
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 459
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
RseI CAYNNNNRTG 1 cut(s) 308
SaqAI TTAA 1 cut(s) 496
SatI GCNGC 4 cut(s) 108, 111, 171, 189
Sau3AI GATC 4 cut(s) 133, 375, 397, 459
SetI ASST 8 cut(s) 33, 72, 100, 132, 193, 285, 372, 470
SfaNI GCATC 1 cut(s) 341
SfcI CTRYAG 1 cut(s) 108
SmiMI CAYNNNNRTG 1 cut(s) 308
Sse9I AATT 1 cut(s) 22
SsiI CCGC 1 cut(s) 204
StyI CCWWGG 1 cut(s) 254
TaaI ACNGT 1 cut(s) 439
TaiI ACGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 423
TasI AATT 1 cut(s) 22
TfiI GAWTC 2 cut(s) 59, 181
Tru1I TTAA 1 cut(s) 496
Tru9I TTAA 1 cut(s) 496
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 4 cut(s) 107, 110, 170, 188
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 4 cut(s) 17, 162, 279, 290
TspRI CASTG 1 cut(s) 54
XcmI CCANNNNNNNNNTGG 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.