pycom02g23230

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011718
Physical Location & Seq
Forward (+)
21365552 .. 21366383
832 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23230.1

Sequence Viewer

Length: 666 bp
ATGGCCATCACCTTCTCAGATCTCTACACCGAGGCCGGTCTCAAGTCCCTCGAGGAGTTCCTCGCCGGAAAATCTTACATCTCCGGAGACAAGCTGACTTTCGATGACATCAAGGTGTACGCCGCCGTGCTGGAAAAGCCTGCCGGTTCATTTGCCAATGTGAGCAAGTGGTACGACGGAATTTCAGCCCAACTCGCTGCAACCTTCCCCGGCAAGGCTGCCGGAGTGAGAGTCAGCAGCGGCAAGGCTGAGGCTGCTGCTCCTGCTGCCGCTGCTGGAGGCGATGACGATGACGATTTGGACCTTTTTGGAGATGAAACCGAGGAGGACAAGAAGGCCGAGGCGGAGATGGCGGCAGCCAAAAAGGCGTCTACCAAGAAGAAGGAGAGTGGGAAATCCTCTGTTCTTTTGGATGTCAAGCCTTGGGACGATGAGACTGACATGAAGAAGCTCGAGGAGGCTGTTCGGAGTGTCGAGAAGGAGGGTCTGTTCTGGGGCGCATCGAAATTGGTTGCTGTTGGTTATGGGATCAAGAAGTTGATGATCATGCTCACCATTGTCGACGACCTTGTTTCCGTCGATGACCTCATCGAAGAGCAGCTTACCGCGGAGCCCCGCAACGAGTATATTCAGAGCTGCGACATTGTTGCCTTCAACAAAATTTAA

Protein Analysis

222

Amino Acids

23.75

Weight (kDa)

4.51

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 134 - 221 9.7e-30 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011718)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12110 AT5G19510
fragaria_vesca FvH4_4g13950
malus_domestica MD02G1099500.v1.1 MD02G1271700.v1.1 MD07G1040800.v1.1
prunus_persica Prupe.5G005100_v2.0.a1
pyrus_communis pycom02g23230 pycom07g02830
rosa_chinensis RchiOBHm_Chr4g0419721
rosa_laevigata RLG00000007801
rosa_multiflora Rmu_sc0016868.1_g000008
rosa_roxburghii Rroxscaffold_5G00362560
rosa_rugosa Rorug04G0160000
rosa_samantha Rh4AG221200 Rh4BG223200 Rh4CG234500 Rh4DG220200
rosa_wichuraiana Rw4G019040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 50
AccI GTMKAC 2 cut(s) 371, 561
AccII CGCG 1 cut(s) 608
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 8 cut(s) 123, 240, 270, 344, 353, 606, 608, 616
AclWI GGATC 1 cut(s) 536
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 180, 660
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 2 cut(s) 119, 173
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 1 cut(s) 655
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
AluBI AGCT 4 cut(s) 94, 451, 601, 636
AluI AGCT 4 cut(s) 94, 451, 601, 636
Alw26I GTCTC 3 cut(s) 44, 81, 428
AlwI GGATC 1 cut(s) 536
Ama87I CYCGRG 2 cut(s) 50, 452
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 3 cut(s) 3, 33, 336
ApoI RAATTY 2 cut(s) 180, 660
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 301
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 1 cut(s) 544
AvaI CYCGRG 2 cut(s) 50, 452
AvaII GGWCC 1 cut(s) 301
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 615
BbvCI CCTCAGC 1 cut(s) 249
BccI CCATC 2 cut(s) 14, 343
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 629, 663
BclI TGATCA 1 cut(s) 543
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 3 cut(s) 44, 81, 428
BglI GCCNNNNNGGC 1 cut(s) 365
BglII AGATCT 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 210
Bme18I GGWCC 1 cut(s) 301
BmeT110I CYCGRG 2 cut(s) 50, 452
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 612
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 1 cut(s) 509
BpmI CTGGAG 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 368
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 6 cut(s) 30, 208, 321, 339, 422, 606
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 4 cut(s) 216, 246, 473, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse118I RCCGGY 2 cut(s) 35, 143
BseAI TCCGGA 1 cut(s) 83
BseDI CCNNGG 6 cut(s) 30, 208, 321, 339, 422, 606
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 30, 240
BseRI GAGGAG 3 cut(s) 68, 338, 470
Bsh1236I CGCG 1 cut(s) 608
BshFI GGCC 3 cut(s) 5, 35, 338
BsiHKCI CYCGRG 2 cut(s) 50, 452
BsiSI CCGG 6 cut(s) 36, 66, 84, 144, 210, 222
BslFI GGGAC 2 cut(s) 31, 440
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 3 cut(s) 44, 81, 428
BsmFI GGGAC 2 cut(s) 31, 440
BsnI GGCC 3 cut(s) 5, 35, 338
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 2 cut(s) 50, 452
Bsp1286I GDGCHC 1 cut(s) 615
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 3 cut(s) 19, 528, 543
BspACI CCGC 8 cut(s) 123, 240, 270, 344, 353, 606, 608, 616
BspANI GGCC 3 cut(s) 5, 35, 338
BspCNI CTCAG 2 cut(s) 29, 241
BspEI TCCGGA 1 cut(s) 83
BspFNI CGCG 1 cut(s) 608
BspLI GGNNCC 1 cut(s) 612
BspPI GGATC 1 cut(s) 536
BspQI GCTCTTC 1 cut(s) 588
BspTNI GGTCTC 1 cut(s) 44
BsrFI RCCGGY 2 cut(s) 35, 143
BssAI RCCGGY 2 cut(s) 35, 143
BssECI CCNNGG 6 cut(s) 30, 208, 321, 339, 422, 606
BssMI GATC 3 cut(s) 19, 528, 543
BssNI GRCGYC 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 422
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 2 cut(s) 16, 249
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 1 cut(s) 608
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 3 cut(s) 22, 531, 546
BstMAI GTCTC 3 cut(s) 44, 81, 428
BstMBI GATC 3 cut(s) 19, 528, 543
BstMWI GCNNNNNNNGC 8 cut(s) 136, 194, 254, 263, 266, 272, 350, 365
BstSCI CCNGG 1 cut(s) 208
BstUI CGCG 1 cut(s) 608
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
BsuRI GGCC 3 cut(s) 5, 35, 338
BtgI CCRYGG 1 cut(s) 606
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 1 cut(s) 418
Cac8I GCNNGC 1 cut(s) 141
CfoI GCGC 1 cut(s) 500
Cfr10I RCCGGY 2 cut(s) 35, 143
Cfr13I GGNCC 1 cut(s) 301
Cfr42I CCGCGG 1 cut(s) 609
CseI GACGC 1 cut(s) 357
Csp6I GTAC 2 cut(s) 118, 172
CviAII CATG 2 cut(s) 442, 547
CviQI GTAC 2 cut(s) 118, 172
DdeI CTNAG 2 cut(s) 16, 249
DpnI GATC 3 cut(s) 21, 530, 545
DpnII GATC 3 cut(s) 19, 528, 543
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
EciI GGCGGA 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 422
Eco24I GRGCYC 1 cut(s) 615
Eco31I GGTCTC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 301
Eco88I CYCGRG 2 cut(s) 50, 452
EcoT14I CCWWGG 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 422
FaeI CATG 2 cut(s) 445, 550
FaiI YATR 4 cut(s) 443, 525, 548, 627
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 2 cut(s) 31, 440
FatI CATG 2 cut(s) 441, 546
FauI CCCGC 1 cut(s) 623
FbaI TGATCA 1 cut(s) 543
FblI GTMKAC 2 cut(s) 371, 561
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 615
GlaI GCGC 1 cut(s) 499
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 3 cut(s) 5, 35, 338
HapII CCGG 6 cut(s) 36, 66, 84, 144, 210, 222
HgaI GACGC 1 cut(s) 357
HhaI GCGC 1 cut(s) 500
Hin1I GRCGYC 1 cut(s) 368
Hin1II CATG 2 cut(s) 445, 550
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 1 cut(s) 231
HpaII CCGG 6 cut(s) 36, 66, 84, 144, 210, 222
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 3 cut(s) 118, 372, 562
Hpy188I TCNGA 3 cut(s) 19, 468, 633
Hpy188III TCNNGA 3 cut(s) 84, 475, 532
Hpy8I GTNNAC 3 cut(s) 118, 372, 562
Hpy99I CGWCG 3 cut(s) 179, 566, 581
HpyAV CCTTC 6 cut(s) 22, 214, 328, 376, 472, 661
HpyCH4V TGCA 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 194, 254, 263, 266, 272, 350, 365
HpyF3I CTNAG 2 cut(s) 16, 249
Hsp92I GRCGYC 1 cut(s) 368
Hsp92II CATG 2 cut(s) 445, 550
HspAI GCGC 1 cut(s) 498
Kpn2I TCCGGA 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 543
KspI CCGCGG 1 cut(s) 609
Kzo9I GATC 3 cut(s) 19, 528, 543
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 2 cut(s) 265, 610
LweI GCATC 1 cut(s) 509
MalI GATC 3 cut(s) 21, 530, 545
MboI GATC 3 cut(s) 19, 528, 543
MboII GAAGA 3 cut(s) 391, 457, 605
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 615
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 180, 506, 660
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 240
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 83
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 664
MslI CAYNNNNRTG 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 240, 272, 608
MspI CCGG 6 cut(s) 36, 66, 84, 144, 210, 222
MspR9I CCNGG 1 cut(s) 210
MvnI CGCG 1 cut(s) 608
MwoI GCNNNNNNNGC 8 cut(s) 136, 194, 254, 263, 266, 272, 350, 365
NciI CCSGG 1 cut(s) 210
NdeII GATC 3 cut(s) 19, 528, 543
NlaIII CATG 2 cut(s) 445, 550
NlaIV GGNNCC 1 cut(s) 612
NmeAIII GCCGAG 1 cut(s) 364
PaeR7I CTCGAG 2 cut(s) 50, 452
PciSI GCTCTTC 1 cut(s) 588
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 612
PspPI GGNCC 1 cut(s) 301
PspXI VCTCGAGB 2 cut(s) 50, 452
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 2 cut(s) 119, 173
RsaNI GTAC 2 cut(s) 118, 172
RseI CAYNNNNRTG 1 cut(s) 113
SacII CCGCGG 1 cut(s) 609
SalI GTCGAC 1 cut(s) 560
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 1 cut(s) 664
Sau3AI GATC 3 cut(s) 19, 528, 543
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 1 cut(s) 615
SfaNI GCATC 1 cut(s) 509
Sfr274I CTCGAG 2 cut(s) 50, 452
Sfr303I CCGCGG 1 cut(s) 609
SgrBI CCGCGG 1 cut(s) 609
SinI GGWCC 1 cut(s) 301
SlaI CTCGAG 2 cut(s) 50, 452
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 3 cut(s) 41, 50, 452
SmoI CTYRAG 3 cut(s) 41, 50, 452
Sse9I AATT 3 cut(s) 180, 506, 660
SsiI CCGC 8 cut(s) 123, 240, 270, 344, 353, 606, 608, 616
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 1 cut(s) 422
TaqI TCGA 8 cut(s) 51, 102, 453, 474, 503, 561, 579, 591
TasI AATT 3 cut(s) 180, 506, 660
TauI GCSGC 4 cut(s) 125, 243, 272, 356
Tru1I TTAA 1 cut(s) 664
Tru9I TTAA 1 cut(s) 664
TspDTI ATGAA 3 cut(s) 138, 330, 458
TspGWI ACGGA 2 cut(s) 192, 565
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 2 cut(s) 180, 660
XhoI CTCGAG 2 cut(s) 50, 452
XmiI GTMKAC 2 cut(s) 371, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.