AT5G14020

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003879
Physical Location & Seq
Reverse (-)
4523421 .. 4526167
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14020.5

Sequence Viewer

Length: 1206 bp
ATGGGGTGTGCTCAATCTACTATTGCTGTTGTGGGAAGGAAGAACAAGAAGAGGATCATTCAAGAATCTGTAGTGTTTGTTCTACAGTTCCGTGTTCCTGTTCAGTCTGATCTTCAGCGCCAACTCAAAGGTGTTTCTCCCAAGACAACCATCAATCGGTTGACATGTTTAAGAAATCAGATTGAGTTGGTAGCTGAGGACACAGGGGGTTCAGCCATCTCTGAGTTGCGTACAGCTTTGGAGGAGTACTTGTCACTTCTATCCGGTCTTATCAAGAAAAGTAAAGATGGAATGGAGGGCTGCGTAGAGCTCAAATGGAGGACCCTTGGCGATGGTCGACGAGCAGAGATATGTTGCACAAACCTTTGGATGGAGATGTTGATAGTGATCCACATGATGGCTGCTTTGGCATTGACTGAAGCTAACTCTCTCATGATTCCAAAGGATTGCTATGCTTCTGGTTCTGGCAATGGCGTCCGTGTGGTCTCCACTGATTGCAGGAGAGATGCGGTTGACTTGCTGCTCAAGGCATCTGGATACTTGGAGTTCTGCGTCCGAGAGATTCTGACTCAACTTCCTCATGATATAAAGAGTAAGTTGCCAGATGATATGCAGGAAAGTGTATTACAAACTCTCTCTATACAAGCACTAGGCCAGGGAACTGAGATTCAACTTGGATTAGCAGTTGATAGTCAGAAAGCAACATTGTCTGTGAAGAGGAGAATTGCATGTGAGCAAGTCATCTACTTCACTCAGGCATATCACTGCTTGTCTAGTTGTGAAGCAGTTAGCCATGGATGCGCCAAGAAACTTCTCCGGTTTATCTACTGGAAGTTTCTGGAAGCAAAGGCTGCAGCATACTACTACCATGGACTGGTAACAGACAAGGGAAGTGAGCCGGCTTGTCACTTGAGCGCTGTGTGTTGTTTCCTTGCAGCTGCAGAGATCCTAGGAGAAAGCAAAAAAGCCTGTCTTAGCTTCTGCTTAGCTCCTCCTGTCACAAGGGCTCCTCCTATGTGGGGTGTTATGAAGCATTTGAGCCAGAAGATTCCAGAAGTTGCTTTTAGAAAGTCGCAGACATATGGATACTTGCTTGAAGAAGAAGAAAAGGCAATGCAGTGTTTGCCAGAGCTTCCAGACTTTCAGTTGTCATTGAGACCCGACGACTTTGAACTGCCTGAAATAGAGGATGTCTCTTCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

401

Amino Acids

44.47

Weight (kDa)

6.67

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 48 - 298 8.3e-13 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 397, 874
AccI GTMKAC 1 cut(s) 337
AciI CCGC 1 cut(s) 509
AclWI GGATC 3 cut(s) 62, 382, 940
AcuI CTGAAG 2 cut(s) 98, 438
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 2 cut(s) 232, 248
AfeI AGCGCT 1 cut(s) 916
AfiI CCNNNNNNNGG 5 cut(s) 156, 370, 397, 874, 1019
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 4 cut(s) 62, 671, 1097, 1172
AjnI CCWGG 1 cut(s) 654
AluBI AGCT 8 cut(s) 194, 236, 310, 422, 938, 978, 989, 1132
AluI AGCT 8 cut(s) 194, 236, 310, 422, 938, 978, 989, 1132
Alw21I GWGCWC 2 cut(s) 13, 312
Alw26I GTCTC 3 cut(s) 490, 1150, 1198
AlwI GGATC 3 cut(s) 62, 382, 940
Aor51HI AGCGCT 1 cut(s) 916
AoxI GGCC 1 cut(s) 652
ApeKI GCWGC 7 cut(s) 300, 401, 520, 851, 854, 935, 938
AspA2I CCTAGG 1 cut(s) 949
AspLEI GCGC 3 cut(s) 120, 803, 917
AspS9I GGNCC 1 cut(s) 321
AvaII GGWCC 1 cut(s) 321
AvrII CCTAGG 1 cut(s) 949
BanII GRGCYC 2 cut(s) 312, 1009
Bbv12I GWGCWC 2 cut(s) 13, 312
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 7 cut(s) 287, 388, 507, 838, 866, 925, 947
BccI CCATC 6 cut(s) 158, 224, 281, 326, 364, 391
BciT130I CCWGG 1 cut(s) 656
BciVI GTATCC 2 cut(s) 530, 1079
BcoDI GTCTC 3 cut(s) 490, 1150, 1198
BfaI CTAG 3 cut(s) 650, 774, 950
BfmI CTRYAG 4 cut(s) 69, 83, 852, 939
BfoI RGCGCY 2 cut(s) 121, 918
BfuI GTATCC 2 cut(s) 530, 1079
BisI GCNGC 7 cut(s) 301, 402, 521, 852, 855, 936, 939
BlnI CCTAGG 1 cut(s) 949
BlpI GCTNAGC 1 cut(s) 985
BlsI GCNGC 7 cut(s) 302, 403, 522, 853, 856, 937, 940
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 1 cut(s) 656
Bme18I GGWCC 1 cut(s) 321
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 2 cut(s) 323, 1008
BmrFI CCNGG 1 cut(s) 656
BmsI GCATC 3 cut(s) 496, 539, 788
BplI GAGNNNNNCTC 1 cut(s) 1178
Bpu10I CCTNAGC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 985
BpuEI CTTGAG 2 cut(s) 509, 931
BsaBI GATNNNNATC 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 474
BsaI GGTCTC 2 cut(s) 490, 1150
BsaJI CCNNGG 5 cut(s) 325, 655, 793, 868, 949
BsaWI WCCGGW 2 cut(s) 263, 816
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 991, 1021
Bsc4I CCNNNNNNNGG 5 cut(s) 156, 370, 397, 874, 1019
Bse118I RCCGGY 1 cut(s) 898
Bse1I ACTGG 2 cut(s) 833, 879
Bse3DI GCAATG 2 cut(s) 475, 1119
Bse8I GATNNNNATC 1 cut(s) 386
BseBI CCWGG 1 cut(s) 656
BseDI CCNNGG 5 cut(s) 325, 655, 793, 868, 949
BseGI GGATG 3 cut(s) 375, 803, 1195
BseJI GATNNNNATC 1 cut(s) 386
BseLI CCNNNNNNNGG 5 cut(s) 156, 370, 397, 874, 1019
BseMI GCAATG 2 cut(s) 475, 1119
BseMII CTCAG 4 cut(s) 186, 213, 654, 767
BseNI ACTGG 2 cut(s) 833, 879
BseRI GAGGAG 4 cut(s) 257, 733, 981, 999
BseXI GCAGC 7 cut(s) 287, 388, 507, 838, 866, 925, 947
BshFI GGCC 1 cut(s) 654
BsiHKAI GWGCWC 2 cut(s) 13, 312
BsiSI CCGG 3 cut(s) 264, 817, 899
BslI CCNNNNNNNGG 5 cut(s) 156, 370, 397, 874, 1019
BsmAI GTCTC 3 cut(s) 490, 1150, 1198
BsnI GGCC 1 cut(s) 654
Bso31I GGTCTC 2 cut(s) 490, 1150
Bsp1286I GDGCHC 3 cut(s) 13, 312, 1009
Bsp143I GATC 4 cut(s) 54, 109, 387, 945
Bsp1720I GCTNAGC 1 cut(s) 985
Bsp19I CCATGG 2 cut(s) 793, 868
BspACI CCGC 1 cut(s) 509
BspANI GGCC 1 cut(s) 654
BspCNI CTCAG 4 cut(s) 187, 214, 655, 766
BspHI TCATGA 3 cut(s) 432, 580, 1202
BspLI GGNNCC 2 cut(s) 323, 1008
BspMAI CTGCAG 2 cut(s) 856, 943
BspPI GGATC 3 cut(s) 62, 382, 940
BspTNI GGTCTC 2 cut(s) 490, 1150
BsrDI GCAATG 2 cut(s) 475, 1119
BsrFI RCCGGY 1 cut(s) 898
BsrI ACTGG 2 cut(s) 833, 879
BssAI RCCGGY 1 cut(s) 898
BssECI CCNNGG 5 cut(s) 325, 655, 793, 868, 949
BssMI GATC 4 cut(s) 54, 109, 387, 945
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 4 cut(s) 325, 793, 868, 949
Bst2UI CCWGG 1 cut(s) 656
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 3 cut(s) 44, 710, 1201
BstACI GRCGYC 1 cut(s) 474
BstAPI GCANNNNNTGC 2 cut(s) 851, 1123
BstC8I GCNNGC 1 cut(s) 900
BstDEI CTNAG 6 cut(s) 195, 222, 663, 753, 974, 985
BstDSI CCRYGG 2 cut(s) 793, 868
BstF5I GGATG 3 cut(s) 375, 803, 1195
BstH2I RGCGCY 2 cut(s) 121, 918
BstHHI GCGC 3 cut(s) 120, 803, 917
BstKTI GATC 4 cut(s) 57, 112, 390, 948
BstMAI GTCTC 3 cut(s) 490, 1150, 1198
BstMBI GATC 4 cut(s) 54, 109, 387, 945
BstMWI GCNNNNNNNGC 4 cut(s) 407, 798, 851, 1123
BstNI CCWGG 1 cut(s) 656
BstNSI RCATGY 2 cut(s) 168, 732
BstSCI CCNGG 1 cut(s) 654
BstSFI CTRYAG 4 cut(s) 69, 83, 852, 939
BstV1I GCAGC 7 cut(s) 287, 388, 507, 838, 866, 925, 947
BstX2I RGATCY 1 cut(s) 945
BstYI RGATCY 1 cut(s) 945
BsuI GTATCC 2 cut(s) 530, 1079
BsuRI GGCC 1 cut(s) 654
BtgI CCRYGG 2 cut(s) 793, 868
BtgZI GCGATG 1 cut(s) 345
BtsCI GGATG 3 cut(s) 375, 803, 1195
BtsI GCAGTG 2 cut(s) 763, 1124
BtsIMutI CAGTG 3 cut(s) 489, 763, 1124
Cac8I GCNNGC 1 cut(s) 900
CciI TCATGA 3 cut(s) 432, 580, 1202
CfoI GCGC 3 cut(s) 120, 803, 917
Cfr10I RCCGGY 1 cut(s) 898
Cfr13I GGNCC 1 cut(s) 321
CseI GACGC 2 cut(s) 463, 541
Csp6I GTAC 2 cut(s) 231, 247
CviAII CATG 8 cut(s) 165, 394, 433, 581, 729, 794, 869, 1203
CviQI GTAC 2 cut(s) 231, 247
DdeI CTNAG 6 cut(s) 195, 222, 663, 753, 974, 985
DpnI GATC 4 cut(s) 56, 111, 389, 947
DpnII GATC 4 cut(s) 54, 109, 387, 945
Eam1104I CTCTTC 3 cut(s) 44, 710, 1201
EarI CTCTTC 3 cut(s) 44, 710, 1201
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 4 cut(s) 325, 793, 868, 949
Eco24I GRGCYC 2 cut(s) 312, 1009
Eco31I GGTCTC 2 cut(s) 490, 1150
Eco47I GGWCC 1 cut(s) 321
Eco47III AGCGCT 1 cut(s) 916
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 98, 438
EcoICRI GAGCTC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 654
EcoT14I CCWWGG 4 cut(s) 325, 793, 868, 949
EcoT38I GRGCYC 2 cut(s) 312, 1009
ErhI CCWWGG 4 cut(s) 325, 793, 868, 949
FaeI CATG 8 cut(s) 168, 397, 436, 584, 732, 797, 872, 1206
FalI AAGNNNNNCTT 2 cut(s) 957, 989
FatI CATG 8 cut(s) 164, 393, 432, 580, 728, 793, 868, 1202
FauNDI CATATG 1 cut(s) 1081
FblI GTMKAC 1 cut(s) 337
Fnu4HI GCNGC 7 cut(s) 301, 402, 521, 852, 855, 936, 939
FokI GGATG 3 cut(s) 382, 810, 1202
FriOI GRGCYC 2 cut(s) 312, 1009
Fsp4HI GCNGC 7 cut(s) 301, 402, 521, 852, 855, 936, 939
FspBI CTAG 3 cut(s) 650, 774, 950
GlaI GCGC 3 cut(s) 119, 802, 916
GluI GCNGC 7 cut(s) 301, 402, 521, 852, 855, 936, 939
HaeII RGCGCY 2 cut(s) 121, 918
HaeIII GGCC 1 cut(s) 654
HapII CCGG 3 cut(s) 264, 817, 899
HgaI GACGC 2 cut(s) 463, 541
HhaI GCGC 3 cut(s) 120, 803, 917
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 8 cut(s) 168, 397, 436, 584, 732, 797, 872, 1206
Hin6I GCGC 3 cut(s) 118, 801, 915
HinP1I GCGC 3 cut(s) 118, 801, 915
HincII GTYRAC 3 cut(s) 162, 338, 514
HindII GTYRAC 3 cut(s) 162, 338, 514
HinfI GANTC 6 cut(s) 65, 436, 562, 568, 667, 1048
HpaII CCGG 3 cut(s) 264, 817, 899
Hpy166II GTNNAC 3 cut(s) 162, 338, 514
Hpy188I TCNGA 6 cut(s) 109, 180, 223, 557, 567, 696
Hpy188III TCNNGA 9 cut(s) 62, 274, 433, 534, 581, 839, 1052, 1136, 1203
Hpy8I GTNNAC 3 cut(s) 162, 338, 514
Hpy99I CGWCG 2 cut(s) 342, 1166
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 8 cut(s) 357, 498, 613, 728, 854, 935, 941, 1117
HpyF10VI GCNNNNNNNGC 4 cut(s) 407, 798, 851, 1123
HpyF3I CTNAG 6 cut(s) 195, 222, 663, 753, 974, 985
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 8 cut(s) 168, 397, 436, 584, 732, 797, 872, 1206
HspAI GCGC 3 cut(s) 118, 801, 915
KroI GCCGGC 1 cut(s) 898
KroNI GCCGGC 1 cut(s) 900
Kzo9I GATC 4 cut(s) 54, 109, 387, 945
LmnI GCTCC 2 cut(s) 994, 1012
Lsp1109I GCAGC 7 cut(s) 287, 388, 507, 838, 866, 925, 947
LweI GCATC 3 cut(s) 496, 539, 788
MaeI CTAG 3 cut(s) 650, 774, 950
MaeIII GTNAC 4 cut(s) 252, 877, 905, 997
MalI GATC 4 cut(s) 56, 111, 389, 947
MboI GATC 4 cut(s) 54, 109, 387, 945
MboII GAAGA 9 cut(s) 52, 61, 104, 727, 1057, 1109, 1112, 1115, 1188
MflI RGATCY 1 cut(s) 945
MhlI GDGCHC 3 cut(s) 13, 312, 1009
MluCI AATT 1 cut(s) 723
MlyI GAGTC 1 cut(s) 562
MroNI GCCGGC 1 cut(s) 898
MseI TTAA 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 3 cut(s) 264, 817, 899
MspR9I CCNGG 1 cut(s) 656
MvaI CCWGG 1 cut(s) 656
MwoI GCNNNNNNNGC 4 cut(s) 407, 798, 851, 1123
NaeI GCCGGC 1 cut(s) 900
NcoI CCATGG 2 cut(s) 793, 868
NdeI CATATG 1 cut(s) 1081
NdeII GATC 4 cut(s) 54, 109, 387, 945
NgoMIV GCCGGC 1 cut(s) 898
NlaIII CATG 8 cut(s) 168, 397, 436, 584, 732, 797, 872, 1206
NlaIV GGNNCC 2 cut(s) 323, 1008
NmuCI GTSAC 3 cut(s) 252, 905, 997
NspI RCATGY 2 cut(s) 168, 732
PagI TCATGA 3 cut(s) 432, 580, 1202
PciI ACATGT 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 900
PfeI GAWTC 5 cut(s) 65, 436, 562, 667, 1048
PflMI CCANNNNNTGG 2 cut(s) 397, 874
PkrI GCNGC 7 cut(s) 302, 403, 522, 853, 856, 937, 940
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
PpuMI RGGWCCY 1 cut(s) 321
PscI ACATGT 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 654
PspGI CCWGG 1 cut(s) 654
PspN4I GGNNCC 2 cut(s) 323, 1008
PspPI GGNCC 1 cut(s) 321
PspPPI RGGWCCY 1 cut(s) 321
PsrI GAACNNNNNNTAC 2 cut(s) 63, 95
PstI CTGCAG 2 cut(s) 856, 943
PsuI RGATCY 1 cut(s) 945
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 2 cut(s) 232, 248
RsaNI GTAC 2 cut(s) 231, 247
SacI GAGCTC 1 cut(s) 312
SalI GTCGAC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 170
SatI GCNGC 7 cut(s) 301, 402, 521, 852, 855, 936, 939
Sau3AI GATC 4 cut(s) 54, 109, 387, 945
Sau96I GGNCC 1 cut(s) 321
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 1 cut(s) 562
ScrFI CCNGG 1 cut(s) 656
SduI GDGCHC 3 cut(s) 13, 312, 1009
SfaNI GCATC 3 cut(s) 496, 539, 788
SfcI CTRYAG 4 cut(s) 69, 83, 852, 939
SinI GGWCC 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 524, 910
SmoI CTYRAG 2 cut(s) 524, 910
Sse9I AATT 1 cut(s) 723
SsiI CCGC 1 cut(s) 509
SspMI CTAG 3 cut(s) 650, 774, 950
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 1 cut(s) 654
StyI CCWWGG 4 cut(s) 325, 793, 868, 949
TaaI ACNGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 337
TasI AATT 1 cut(s) 723
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 5 cut(s) 65, 436, 562, 667, 1048
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TscAI CASTG 3 cut(s) 496, 770, 1124
TseFI GTSAC 3 cut(s) 252, 905, 997
TseI GCWGC 7 cut(s) 300, 401, 520, 851, 854, 935, 938
Tsp45I GTSAC 3 cut(s) 252, 905, 997
TspDTI ATGAA 1 cut(s) 1043
TspGWI ACGGA 2 cut(s) 80, 467
TspRI CASTG 3 cut(s) 496, 770, 1124
Van91I CCANNNNNTGG 2 cut(s) 397, 874
VpaK11BI GGWCC 1 cut(s) 321
XceI RCATGY 2 cut(s) 168, 732
XmaJI CCTAGG 1 cut(s) 949
XmiI GTMKAC 1 cut(s) 337
XspI CTAG 3 cut(s) 650, 774, 950
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.