Rh2AG670900

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003879
Physical Location & Seq
Reverse (-)
88448827 .. 88452363
3537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG670900.1

Sequence Viewer

Length: 1275 bp
ATGGGGTGTGCTAGTTCTATATATGCTGCTGTTGGAACAAAGAAGAAGTTGTCTATCCCTGAAGTTGTCGTCTTCGTCCCATCCACTCGCATTCCTGCACAATCTGATCTTCAAAAGCAGCTTAGAGGCCTAATTCCAAAAGATATTGCTGACAGATTGTCTTCGCTTCGTAATCAGATTGTCTTTGTGGCAGAGGACACTGATGGATCTGCTATACCTGAACTCCGCCGAGGGCTGGAGGAATACTTGTCTCTTTTAATTGGCCTCACTAAAAAAGAATATGGTCTTGAGGGATTGGTTGAATTCAAGTGGAGAAGTTTTGCGGGTGGGAAACAACAGCAGGAAGCCTCCTCCTCATGTATAGCAAACTCTTGGTTTGAAGTACTGTCTGTTGTTCACATGATGGCTATGCTTGCGTTGTCGGAAGCTGACACATTGATGATTCCAAAGGACCATTCTGGCTCTAATATAAGGACTGTATCTTCAGATTGCAAGAGGGAAGCTGTGGACTTATTGCTTAAGGCAACAGGGTATTTGGAATTCTGTGTCCGGGATGTACTGGTTCATATACCACCAGAAATCAAGAAAAATTTTCCGAAAGATTTGCAGGATGGTGTGTTGGAGGCCATTTCCTTTCAAACTCTTGGCCAGGGAACTGAAATTCAGCTTGGTTTAGCTGTTGACTGTCAGAAGGCTACTTTATCAGTTAAAAGGAGATTGGCCTGTGAACAATTGAGCTATTTCAGCCAGGCTTATCACTGCTTGTCAGGATATGATCACATCAACAACGGGTATGCAAAGAAGCATATGTGGTTCATCAAATGGAAATTTCTGGAGTCAAAGGCTGCAGCTTACTACTTCCATGGTCTAATGGTTGACAAGGGTAATGAACCATCATGCCATGTTAGCGCTGTATGTTGTTTTCTTGCCGCAGAAGAAATTCTATCAGAGAGCAAGAAAGCTTGCTTTACCTTTTGCCTTGCAGCTCCAGTTACCAGGGCTTCTCCACTGTGGGGAGCTATGAAGCATTTGCATCAGAAAATCCCTGAAGTTGCAGCAAGGAAATGCCAGATGTATGGCTACCTCTTAGAACAAGACAAAGTTCTTCAAGCATTGCCTGACCTACCAGAATTTCAACTGTCATTAAGACCTGATGACTATCAACTACCTGAAATCGACCCAGCTTGGGAGTCGGAAAAATGGGAAACTCAAAGCCAGAGCTTAAAAGAGCACCTTGATGATAGCGATGACAACGATGAGCCTGAAACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

424

Amino Acids

47.51

Weight (kDa)

5.49

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 50 - 319 3.1e-10 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 226, 323, 930
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 7 cut(s) 302, 539, 589, 660, 827, 939, 1130
AcuI CTGAAG 3 cut(s) 81, 468, 1068
AfaI GTAC 2 cut(s) 384, 558
AfeI AGCGCT 1 cut(s) 910
AfiI CCNNNNNNNGG 3 cut(s) 235, 1013, 1186
AflII CTTAAG 1 cut(s) 518
AgsI TTSAA 7 cut(s) 113, 302, 307, 380, 638, 1109, 1136
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 3 cut(s) 648, 747, 995
AjuI GAANNNNNNNTTGG 4 cut(s) 439, 471, 651, 683
Alw21I GWGCWC 1 cut(s) 1233
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 910
AoxI GGCC 5 cut(s) 127, 262, 624, 646, 720
ApeKI GCWGC 6 cut(s) 26, 118, 845, 848, 983, 1055
ApoI RAATTY 7 cut(s) 302, 539, 589, 660, 827, 939, 1130
Asp700I GAANNNNTTC 1 cut(s) 939
AspLEI GCGC 1 cut(s) 911
AspS9I GGNCC 1 cut(s) 451
AsuC2I CCSGG 1 cut(s) 551
AvaII GGWCC 1 cut(s) 451
BalI TGGCCA 1 cut(s) 648
BbsI GAAGAC 2 cut(s) 64, 153
Bbv12I GWGCWC 1 cut(s) 1233
BbvI GCAGC 6 cut(s) 13, 130, 832, 860, 995, 1067
BccI CCATC 5 cut(s) 88, 197, 397, 605, 901
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 3 cut(s) 650, 749, 997
BclI TGATCA 1 cut(s) 775
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 846
BfoI RGCGCY 1 cut(s) 912
BfrI CTTAAG 1 cut(s) 518
BisI GCNGC 7 cut(s) 27, 119, 846, 849, 930, 984, 1056
BlsI GCNGC 7 cut(s) 28, 120, 847, 850, 931, 985, 1057
BmcAI AGTACT 1 cut(s) 384
Bme1390I CCNGG 4 cut(s) 551, 650, 749, 997
Bme18I GGWCC 1 cut(s) 451
BmeRI GACNNNNNGTC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 451
BmrFI CCNGG 4 cut(s) 551, 650, 749, 997
BmsI GCATC 1 cut(s) 1042
BpiI GAAGAC 2 cut(s) 64, 153
BpmI CTGGAG 3 cut(s) 257, 854, 972
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 1158
BsaJI CCNNGG 4 cut(s) 229, 649, 862, 996
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 1013, 1186
Bse1I ACTGG 2 cut(s) 564, 989
Bse3DI GCAATG 1 cut(s) 1112
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 3 cut(s) 650, 749, 997
BseDI CCNNGG 4 cut(s) 229, 649, 862, 996
BseGI GGATG 3 cut(s) 80, 559, 616
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 3 cut(s) 235, 1013, 1186
BseMI GCAATG 1 cut(s) 1112
BseMII CTCAG 1 cut(s) 1260
BseNI ACTGG 2 cut(s) 564, 989
BseRI GAGGAG 2 cut(s) 340, 343
BseXI GCAGC 6 cut(s) 13, 130, 832, 860, 995, 1067
BseYI CCCAGC 1 cut(s) 1180
BsgI GTGCAG 1 cut(s) 81
BshFI GGCC 5 cut(s) 129, 264, 626, 648, 722
BsiHKAI GWGCWC 1 cut(s) 1233
BsiSI CCGG 1 cut(s) 550
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 235, 1013, 1186
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 5 cut(s) 129, 264, 626, 648, 722
Bsp1286I GDGCHC 1 cut(s) 1233
Bsp143I GATC 3 cut(s) 106, 206, 775
Bsp19I CCATGG 1 cut(s) 862
BspACI CCGC 3 cut(s) 226, 323, 930
BspANI GGCC 5 cut(s) 129, 264, 626, 648, 722
BspCNI CTCAG 1 cut(s) 1261
BspMAI CTGCAG 1 cut(s) 850
BspPI GGATC 1 cut(s) 214
BspTI CTTAAG 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 1112
BsrI ACTGG 2 cut(s) 564, 989
BssECI CCNNGG 4 cut(s) 229, 649, 862, 996
BssMI GATC 3 cut(s) 106, 206, 775
BssT1I CCWWGG 1 cut(s) 862
Bst2UI CCWGG 3 cut(s) 650, 749, 997
Bst4CI ACNGT 5 cut(s) 387, 478, 686, 1011, 1140
BstAFI CTTAAG 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 414, 964
BstDEI CTNAG 3 cut(s) 122, 1087, 1269
BstDSI CCRYGG 1 cut(s) 862
BstF5I GGATG 3 cut(s) 80, 559, 616
BstH2I RGCGCY 1 cut(s) 912
BstHHI GCGC 1 cut(s) 911
BstKTI GATC 3 cut(s) 109, 209, 778
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 3 cut(s) 106, 206, 775
BstMWI GCNNNNNNNGC 3 cut(s) 413, 744, 906
BstNI CCWGG 3 cut(s) 650, 749, 997
BstSCI CCNGG 4 cut(s) 549, 648, 747, 995
BstSFI CTRYAG 1 cut(s) 846
BstV1I GCAGC 6 cut(s) 13, 130, 832, 860, 995, 1067
BstV2I GAAGAC 2 cut(s) 64, 153
BstX2I RGATCY 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 1076
BstYI RGATCY 1 cut(s) 206
BsuRI GGCC 5 cut(s) 129, 264, 626, 648, 722
BtgI CCRYGG 1 cut(s) 862
BtgZI GCGATG 1 cut(s) 1260
BtsCI GGATG 3 cut(s) 80, 559, 616
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 3 cut(s) 198, 757, 1007
Cac8I GCNNGC 2 cut(s) 414, 964
CfoI GCGC 1 cut(s) 911
Cfr13I GGNCC 1 cut(s) 451
Csp6I GTAC 2 cut(s) 383, 557
CviAII CATG 5 cut(s) 357, 400, 863, 897, 902
CviQI GTAC 2 cut(s) 383, 557
DdeI CTNAG 3 cut(s) 122, 1087, 1269
DpnI GATC 3 cut(s) 108, 208, 777
DpnII GATC 3 cut(s) 106, 206, 775
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 646
Eam1105I GACNNNNNGTC 1 cut(s) 157
EciI GGCGGA 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 862
Eco147I AGGCCT 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 451
Eco47III AGCGCT 1 cut(s) 910
Eco57I CTGAAG 3 cut(s) 81, 468, 1068
EcoRI GAATTC 2 cut(s) 302, 539
EcoRII CCWGG 3 cut(s) 648, 747, 995
EcoT14I CCWWGG 1 cut(s) 862
ErhI CCWWGG 1 cut(s) 862
FaeI CATG 5 cut(s) 360, 403, 866, 900, 905
FalI AAGNNNNNCTT 2 cut(s) 1218, 1250
FaqI GGGAC 1 cut(s) 62
FatI CATG 5 cut(s) 356, 399, 862, 896, 901
FauI CCCGC 1 cut(s) 316
FauNDI CATATG 1 cut(s) 807
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 7 cut(s) 27, 119, 846, 849, 930, 984, 1056
FokI GGATG 3 cut(s) 67, 566, 623
Fsp4HI GCNGC 7 cut(s) 27, 119, 846, 849, 930, 984, 1056
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 910
GluI GCNGC 7 cut(s) 27, 119, 846, 849, 930, 984, 1056
GsaI CCCAGC 1 cut(s) 1184
GsuI CTGGAG 3 cut(s) 257, 854, 972
HaeII RGCGCY 1 cut(s) 912
HaeIII GGCC 5 cut(s) 129, 264, 626, 648, 722
HapII CCGG 1 cut(s) 550
HhaI GCGC 1 cut(s) 911
Hin1II CATG 5 cut(s) 360, 403, 866, 900, 905
Hin6I GCGC 1 cut(s) 909
HinP1I GCGC 1 cut(s) 909
HincII GTYRAC 2 cut(s) 682, 877
HindII GTYRAC 2 cut(s) 682, 877
HindIII AAGCTT 1 cut(s) 960
HinfI GANTC 3 cut(s) 442, 836, 1190
HpaII CCGG 1 cut(s) 550
Hpy166II GTNNAC 5 cut(s) 397, 508, 682, 728, 877
Hpy188I TCNGA 9 cut(s) 106, 177, 424, 487, 597, 690, 949, 1038, 1195
Hpy188III TCNNGA 4 cut(s) 287, 583, 768, 833
Hpy8I GTNNAC 5 cut(s) 397, 508, 682, 728, 877
HpyAV CCTTC 1 cut(s) 685
HpyCH4III ACNGT 5 cut(s) 387, 478, 686, 1011, 1140
HpyCH4V TGCA 8 cut(s) 98, 492, 607, 797, 848, 983, 1033, 1055
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 744, 906
HpyF3I CTNAG 3 cut(s) 122, 1087, 1269
Hsp92II CATG 5 cut(s) 360, 403, 866, 900, 905
HspAI GCGC 1 cut(s) 909
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 3 cut(s) 106, 206, 775
LmnI GCTCC 2 cut(s) 991, 1016
Lsp1109I GCAGC 6 cut(s) 13, 130, 832, 860, 995, 1067
LweI GCATC 1 cut(s) 1042
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 991
MalI GATC 3 cut(s) 108, 208, 777
MboI GATC 3 cut(s) 106, 206, 775
MboII GAAGA 7 cut(s) 55, 64, 101, 153, 474, 947, 1097
MfeI CAATTG 1 cut(s) 731
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 1233
MlsI TGGCCA 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 2 cut(s) 845, 1199
MmeI TCCRAC 4 cut(s) 13, 402, 600, 1173
Mox20I TGGCCA 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 939
MscI TGGCCA 1 cut(s) 648
MseI TTAA 5 cut(s) 257, 519, 708, 1145, 1223
MslI CAYNNNNRTG 2 cut(s) 437, 1236
Msp20I TGGCCA 1 cut(s) 648
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 1 cut(s) 550
MspR9I CCNGG 4 cut(s) 551, 650, 749, 997
MunI CAATTG 1 cut(s) 731
Mva1269I GAATGC 1 cut(s) 90
MvaI CCWGG 3 cut(s) 650, 749, 997
MwoI GCNNNNNNNGC 3 cut(s) 413, 744, 906
NciI CCSGG 1 cut(s) 551
NcoI CCATGG 1 cut(s) 862
NdeI CATATG 1 cut(s) 807
NdeII GATC 3 cut(s) 106, 206, 775
NlaIII CATG 5 cut(s) 360, 403, 866, 900, 905
NmeAIII GCCGAG 1 cut(s) 254
PceI AGGCCT 1 cut(s) 129
PctI GAATGC 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 939
PfeI GAWTC 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 7 cut(s) 28, 120, 847, 850, 931, 985, 1057
PleI GAGTC 2 cut(s) 844, 1198
PpsI GAGTC 2 cut(s) 844, 1198
Psp6I CCWGG 3 cut(s) 648, 747, 995
PspFI CCCAGC 1 cut(s) 1180
PspGI CCWGG 3 cut(s) 648, 747, 995
PspPI GGNCC 1 cut(s) 451
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 2 cut(s) 384, 558
RsaNI GTAC 2 cut(s) 383, 557
RseI CAYNNNNRTG 2 cut(s) 437, 1236
SaqAI TTAA 5 cut(s) 257, 519, 708, 1145, 1223
SatI GCNGC 7 cut(s) 27, 119, 846, 849, 930, 984, 1056
Sau3AI GATC 3 cut(s) 106, 206, 775
Sau96I GGNCC 1 cut(s) 451
ScaI AGTACT 1 cut(s) 384
SchI GAGTC 2 cut(s) 845, 1199
ScrFI CCNGG 4 cut(s) 551, 650, 749, 997
SduI GDGCHC 1 cut(s) 1233
SfaNI GCATC 1 cut(s) 1042
SfcI CTRYAG 1 cut(s) 846
SinI GGWCC 1 cut(s) 451
SmiMI CAYNNNNRTG 2 cut(s) 437, 1236
SmlI CTYRAG 2 cut(s) 287, 518
SmoI CTYRAG 2 cut(s) 287, 518
SseBI AGGCCT 1 cut(s) 129
SsiI CCGC 3 cut(s) 226, 323, 930
SspMI CTAG 1 cut(s) 12
StuI AGGCCT 1 cut(s) 129
StyD4I CCNGG 4 cut(s) 549, 648, 747, 995
StyI CCWWGG 1 cut(s) 862
TaaI ACNGT 5 cut(s) 387, 478, 686, 1011, 1140
TaqI TCGA 1 cut(s) 1176
TatI WGTACW 2 cut(s) 382, 556
TauI GCSGC 1 cut(s) 932
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 5 cut(s) 257, 519, 708, 1145, 1223
Tru9I TTAA 5 cut(s) 257, 519, 708, 1145, 1223
TscAI CASTG 3 cut(s) 205, 764, 1014
TseI GCWGC 6 cut(s) 26, 118, 845, 848, 983, 1055
TspDTI ATGAA 4 cut(s) 554, 805, 903, 1037
TspRI CASTG 3 cut(s) 205, 764, 1014
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 451
XapI RAATTY 7 cut(s) 302, 539, 589, 660, 827, 939, 1130
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.