Rorug02G0592700

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003879
Physical Location & Seq
Forward (+)
71477273 .. 71477791
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0592700.1

Sequence Viewer

Length: 519 bp
ATGTTTGCACTTAAGGATGACTTGGTAGCTGACTATATTCATAATGGGGCATGGAACTTTCCTTCTTTGCTTCAATTTCACTTTCCCGGCATATGTAAGTTAATTGATAATGTTCCTATTTCACTAGTTCCGGATATGCCTGATAAGTTGATTTGGGTTCCTTCATCTTCCGGAGAGCTGACAGCAAAAGAGGCGTTCCATTTTCTTCGCCCACGCCTCCCACTCAGGGATTGGGGTAAGCTCATATGGTCTAAATTCATTATCCCTCGTATTTCTCTTCATTCTTGGAAAGTGTTACGAGGGAGGGTGTTATCAGAGGATTTATTGCAGCGTCGAGGGATTGCTTTGGCTTCCCGTTGTGTTCTTTGTGGTTTGGCTGGTGAATCACTACTGCATATATTTTTGAACTGTTCTTTTGCTGCATCTTTATGGAATAGCTGGGCTTGTTTATTTGAGTTAAGTAACCTGCCATATACCTTGATTGATTTATTAATTACATTGGGGGTGTGTTGGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

172

Amino Acids

19.55

Weight (kDa)

8.26

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 61 - 144 1e-20 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 474
AccIII TCCGGA 2 cut(s) 130, 170
AcsI RAATTY 1 cut(s) 254
AfiI CCNNNNNNNGG 1 cut(s) 226
AflII CTTAAG 1 cut(s) 11
AgsI TTSAA 2 cut(s) 74, 406
AhlI ACTAGT 1 cut(s) 124
AluBI AGCT 4 cut(s) 29, 178, 241, 438
AluI AGCT 4 cut(s) 29, 178, 241, 438
Aor13HI TCCGGA 2 cut(s) 130, 170
ApeKI GCWGC 2 cut(s) 328, 419
ApoI RAATTY 1 cut(s) 254
AseI ATTAAT 1 cut(s) 491
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 392
BbvI GCAGC 2 cut(s) 340, 406
BcnI CCSGG 1 cut(s) 87
BcuI ACTAGT 1 cut(s) 124
BfaI CTAG 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 474
BisI GCNGC 2 cut(s) 329, 420
BlsI GCNGC 2 cut(s) 330, 421
Bme1390I CCNGG 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 87
BsaWI WCCGGW 2 cut(s) 130, 170
Bsc4I CCNNNNNNNGG 1 cut(s) 226
BseAI TCCGGA 2 cut(s) 130, 170
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMII CTCAG 1 cut(s) 238
BseXI GCAGC 2 cut(s) 340, 406
BseYI CCCAGC 1 cut(s) 438
BsiSI CCGG 3 cut(s) 87, 131, 171
BslI CCNNNNNNNGG 1 cut(s) 226
Bsp13I TCCGGA 2 cut(s) 130, 170
BspCNI CTCAG 1 cut(s) 237
BspEI TCCGGA 2 cut(s) 130, 170
BspLI GGNNCC 1 cut(s) 159
BspMI ACCTGC 1 cut(s) 474
BspTI CTTAAG 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 282
BstAFI CTTAAG 1 cut(s) 11
BstDEI CTNAG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 340, 406
BtsCI GGATG 1 cut(s) 22
BveI ACCTGC 1 cut(s) 474
CseI GACGC 1 cut(s) 320
CviAII CATG 1 cut(s) 51
CviJI RGCY 7 cut(s) 29, 178, 241, 350, 377, 438, 443
CviKI_1 RGCY 7 cut(s) 29, 178, 241, 350, 377, 438, 443
DdeI CTNAG 1 cut(s) 224
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
FaeI CATG 1 cut(s) 54
FalI AAGNNNNNCTT 1 cut(s) 37
FatI CATG 1 cut(s) 50
FauNDI CATATG 2 cut(s) 92, 245
Fnu4HI GCNGC 2 cut(s) 329, 420
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 2 cut(s) 329, 420
FspBI CTAG 1 cut(s) 125
GluI GCNGC 2 cut(s) 329, 420
GsaI CCCAGC 1 cut(s) 442
HapII CCGG 3 cut(s) 87, 131, 171
HgaI GACGC 1 cut(s) 320
Hin1II CATG 1 cut(s) 54
HinfI GANTC 1 cut(s) 383
HpaII CCGG 3 cut(s) 87, 131, 171
HphI GGTGA 1 cut(s) 392
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 131, 171
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 2 cut(s) 72, 171
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 4 cut(s) 8, 328, 394, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 1 cut(s) 515
Hsp92II CATG 1 cut(s) 54
Kpn2I TCCGGA 2 cut(s) 130, 170
LpnPI CCDG 8 cut(s) 100, 144, 153, 184, 211, 363, 424, 479
Lsp1109I GCAGC 2 cut(s) 340, 406
LweI GCATC 1 cut(s) 431
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 2 cut(s) 294, 461
MboII GAAGA 3 cut(s) 159, 197, 269
MluCI AATT 4 cut(s) 74, 102, 254, 492
MmeI TCCRAC 1 cut(s) 491
MnlI CCTC 7 cut(s) 184, 227, 276, 293, 297, 310, 329
MroI TCCGGA 2 cut(s) 130, 170
MseI TTAA 4 cut(s) 12, 101, 458, 491
MslI CAYNNNNRTG 1 cut(s) 427
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 3 cut(s) 87, 131, 171
MspR9I CCNGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 191
NciI CCSGG 1 cut(s) 87
NdeI CATATG 2 cut(s) 92, 245
NlaIII CATG 1 cut(s) 54
NlaIV GGNNCC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 2 cut(s) 330, 421
PshBI ATTAAT 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 438
PspN4I GGNNCC 1 cut(s) 159
RseI CAYNNNNRTG 1 cut(s) 427
SaqAI TTAA 4 cut(s) 12, 101, 458, 491
SatI GCNGC 2 cut(s) 329, 420
ScrFI CCNGG 1 cut(s) 87
SetI ASST 7 cut(s) 31, 180, 243, 440, 468, 479, 518
SfaNI GCATC 1 cut(s) 431
SmiMI CAYNNNNRTG 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SpeI ACTAGT 1 cut(s) 124
Sse9I AATT 4 cut(s) 74, 102, 254, 492
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 1 cut(s) 518
TaqI TCGA 1 cut(s) 334
TasI AATT 4 cut(s) 74, 102, 254, 492
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 4 cut(s) 12, 101, 458, 491
Tru9I TTAA 4 cut(s) 12, 101, 458, 491
TseI GCWGC 2 cut(s) 328, 419
TspDTI ATGAA 4 cut(s) 29, 153, 247, 269
Vha464I CTTAAG 1 cut(s) 11
VspI ATTAAT 1 cut(s) 491
XapI RAATTY 1 cut(s) 254
XcmI CCANNNNNNNNNTGG 1 cut(s) 228
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.