Rorug02G0592600

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003879
Physical Location & Seq
Forward (+)
71463547 .. 71465429
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0592600.1

Sequence Viewer

Length: 279 bp
ATGTCTAGCAGAAGGTCAAGGCAGTCATCGGGTAGTCCATCGATCAAAGATGACCAGATCATCGAGCTCGTCTCCAAGTTGCGCCAGCTCGTTCCTGAGATTCGCGACAGGCGCTCCGATAAGGTATCAGCATCGAAGGTCCTACAAGAGACCTGCAGCTACATCAGAAACTTACACAGAGAGGTTGACGACTTGAGCGAGAGACTTTCCCAACTGCTCGCTACAATTGATGCTGATAGCGCCGAGGCCGCCATTATTAGGAGCTTGATTATGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

92

Amino Acids

10.41

Weight (kDa)

7.93

Isoelectric Point (pI)

83.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_ILI PF23174 17 - 90 1e-33 ILI bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 161
AccII CGCG 1 cut(s) 105
AciI CCGC 1 cut(s) 249
AfiI CCNNNNNNNGG 1 cut(s) 258
AluBI AGCT 4 cut(s) 67, 88, 159, 264
AluI AGCT 4 cut(s) 67, 88, 159, 264
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 76, 143, 196
AoxI GGCC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 156
AspLEI GCGC 3 cut(s) 84, 114, 242
AspS9I GGNCC 1 cut(s) 139
AvaII GGWCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 168
BccI CCATC 1 cut(s) 46
BcoDI GTCTC 3 cut(s) 76, 143, 196
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 154
BfoI RGCGCY 2 cut(s) 115, 243
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 2 cut(s) 157, 249
BlsI GCNGC 2 cut(s) 158, 250
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmsI GCATC 2 cut(s) 140, 220
BplI GAGNNNNNCTC 2 cut(s) 56, 88
BpuEI CTTGAG 1 cut(s) 214
Bsa29I ATCGAT 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 243
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 1 cut(s) 258
BseCI ATCGAT 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 243
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 87
BseXI GCAGC 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 248
BshVI ATCGAT 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 3 cut(s) 76, 143, 196
BsmBI CGTCTC 1 cut(s) 76
BsnI GGCC 1 cut(s) 248
Bso31I GGTCTC 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 2 cut(s) 42, 57
Bsp68I TCGCGA 1 cut(s) 105
BspACI CCGC 1 cut(s) 249
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 88
BspDI ATCGAT 1 cut(s) 41
BspFNI CGCG 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 158
BspMI ACCTGC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 2 cut(s) 42, 57
BstC8I GCNNGC 2 cut(s) 86, 219
BstDEI CTNAG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 105
BstH2I RGCGCY 2 cut(s) 115, 243
BstHHI GCGC 3 cut(s) 84, 114, 242
BstKTI GATC 2 cut(s) 45, 60
BstMAI GTCTC 3 cut(s) 76, 143, 196
BstMBI GATC 2 cut(s) 42, 57
BstMWI GCNNNNNNNGC 3 cut(s) 111, 239, 248
BstSFI CTRYAG 1 cut(s) 154
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 168
Bsu15I ATCGAT 1 cut(s) 41
BsuRI GGCC 1 cut(s) 248
BsuTUI ATCGAT 1 cut(s) 41
BtuMI TCGCGA 1 cut(s) 105
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 2 cut(s) 86, 219
CfoI GCGC 3 cut(s) 84, 114, 242
Cfr13I GGNCC 1 cut(s) 139
ClaI ATCGAT 1 cut(s) 41
CviJI RGCY 5 cut(s) 67, 88, 159, 248, 264
CviKI_1 RGCY 5 cut(s) 67, 88, 159, 248, 264
DdeI CTNAG 1 cut(s) 96
DpnI GATC 2 cut(s) 44, 59
DpnII GATC 2 cut(s) 42, 57
Ecl136II GAGCTC 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 139
Eco53kI GAGCTC 1 cut(s) 67
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 76
FaiI YATR 1 cut(s) 272
Fnu4HI GCNGC 2 cut(s) 157, 249
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 2 cut(s) 157, 249
FspBI CTAG 1 cut(s) 6
GlaI GCGC 3 cut(s) 83, 113, 241
GluI GCNGC 2 cut(s) 157, 249
HaeII RGCGCY 2 cut(s) 115, 243
HaeIII GGCC 1 cut(s) 248
HhaI GCGC 3 cut(s) 84, 114, 242
Hin6I GCGC 3 cut(s) 82, 112, 240
HinP1I GCGC 3 cut(s) 82, 112, 240
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 2 cut(s) 118, 167
Hpy188III TCNNGA 2 cut(s) 95, 104
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 2 cut(s) 6, 130
HpyCH4V TGCA 2 cut(s) 156, 274
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 239, 248
HpyF3I CTNAG 1 cut(s) 96
HspAI GCGC 3 cut(s) 82, 112, 240
Kzo9I GATC 2 cut(s) 42, 57
LmnI GCTCC 2 cut(s) 119, 261
LpnPI CCDG 5 cut(s) 68, 94, 98, 108, 166
Lsp1109I GCAGC 1 cut(s) 168
LweI GCATC 2 cut(s) 140, 220
MaeI CTAG 1 cut(s) 6
MalI GATC 2 cut(s) 44, 59
MboI GATC 2 cut(s) 42, 57
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 1 cut(s) 225
MnlI CCTC 2 cut(s) 175, 238
MunI CAATTG 1 cut(s) 225
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 3 cut(s) 111, 239, 248
NdeII GATC 2 cut(s) 42, 57
NmeAIII GCCGAG 1 cut(s) 268
NruI TCGCGA 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 2 cut(s) 158, 250
PpuMI RGGWCCY 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 139
PspPI GGNCC 1 cut(s) 139
PspPPI RGGWCCY 1 cut(s) 139
PstI CTGCAG 1 cut(s) 158
RruI TCGCGA 1 cut(s) 105
SacI GAGCTC 1 cut(s) 69
SatI GCNGC 2 cut(s) 157, 249
Sau3AI GATC 2 cut(s) 42, 57
Sau96I GGNCC 1 cut(s) 139
SduI GDGCHC 1 cut(s) 69
SetI ASST 9 cut(s) 17, 69, 90, 126, 141, 155, 161, 186, 266
SfaNI GCATC 2 cut(s) 140, 220
SfcI CTRYAG 1 cut(s) 154
SinI GGWCC 1 cut(s) 139
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 1 cut(s) 225
SsiI CCGC 1 cut(s) 249
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 69
TaqI TCGA 3 cut(s) 41, 63, 134
TasI AATT 1 cut(s) 225
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 1 cut(s) 100
TseI GCWGC 1 cut(s) 156
VpaK11BI GGWCC 1 cut(s) 139
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.