RchiOBHm_Chr2g0176271

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003879
Physical Location & Seq
Reverse (-)
88168254 .. 88171280
3027 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54329

Sequence Viewer

Length: 1275 bp
ATGGGGTGTGCTAGTTCTATATATGCTGCTGTTGGAACAAAGAAGAAGTTGTCTATCCCTGAAGTTGTCGTCTTCGTCCCATCCACTCGCATTCCTGCACAATCTGATCTTCAAAAGCAGCTTAGAGGCCTAATTCCAAAAGATATTGCTGACAGATTGTCTTCGCTTCGTAATCAGATTGTCTTTGTGGCAGAGGACACTGATGGATCTGCTATACCTGAACTCCGCCGAGGGCTAGAGGAATACTTGTCTCTTTTAATTGGCCTCACTAAAAAAGAATATGGTCTTGAGGGATTGGTTGAATTCAAGTGGAGAAGTTTAGCGGGTGGGAAACAACAGGAAGCCTCCTCCTCATGTATAGCAAACTCTTGGTTTGAAGTACTGTCTGTTGTTCACATGATGGCTATGCTTGCGTTGTCGGAAGCTGACACATTGACGATTCCAAAGGACCATTCTGGCTCTAATATAAGGACTGTATCTTCAGATTGCAAGAGGGAAGCTGTGGACTTATTGCTTAAGGCAACAGGGTATTTGGAATTCTGTGTCCGGGATGTACTGGTTCATATACCACCTGAAATCAAGAAAAATTTTCCTAAAGATTTGCAGGATGGTGTGTTGGAGGCCATTTCCTTTCAAACTCTTGGCCAGGGAACTGAAATTCAGCTTGGTTTAGCTGTTGACTGTCAGAAGGCTACTTTATCAGTTAAAAGGAGATTGGCCTGTGAACAATTGAGCTATTTCAGCCAGGCTTATCACTGCTTGTCAGGATATGATCACATCAACAACGGGTATGGAAAGAAGCATATGTGGTTCATCAAATGGAAATTTCTGGAGTCAAAGGCTGCAGCTTACTACTTCCATGGTCTAATGGTTGACAAGGGTAATGAACCATCATGCCATGTTAGCGCTGTATGTTGTTTTCTTGCCGCAGAAGAAATTCTATCAGAGAGCAAGAAAGCTTGCTTTACCTTTTGCCTTGCAGCTCCAGTTACCAGGGCTGCTCCACTGTGGGGAGCTATGAAGCATTTGCATCAGAAAATCCCTGAAGTTGCAGCAAGGAAATCCCAGATGTATGGCTACCTCTTAGAACAAGACAAAGTTCTTCAAGCATTGCCTGACCTACCAGAATTTCAACTGTCATTAAGACCTGATGACTATCAACTACCTGAAATCGACCCAGCTTGGGAGTCGGGAAAATGGGAAACTCAAAGCCAGAGCTTAAAAGAGCACCTAGATGATAGCGATGACAACGATGAGACTGAAACTGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

424

Amino Acids

47.33

Weight (kDa)

5.56

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 226, 323, 927
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 7 cut(s) 302, 536, 586, 657, 824, 936, 1127
AcuI CTGAAG 3 cut(s) 81, 465, 1065
AfaI GTAC 2 cut(s) 381, 555
AfeI AGCGCT 1 cut(s) 907
AfiI CCNNNNNNNGG 2 cut(s) 1010, 1183
AflII CTTAAG 1 cut(s) 515
AgsI TTSAA 7 cut(s) 113, 302, 307, 377, 635, 1106, 1133
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 3 cut(s) 645, 744, 992
AjuI GAANNNNNNNTTGG 4 cut(s) 436, 468, 648, 680
Alw21I GWGCWC 1 cut(s) 1230
Alw26I GTCTC 2 cut(s) 255, 1250
AlwI GGATC 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 907
AoxI GGCC 5 cut(s) 127, 262, 621, 643, 717
ApeKI GCWGC 7 cut(s) 26, 118, 842, 845, 980, 998, 1052
ApoI RAATTY 7 cut(s) 302, 536, 586, 657, 824, 936, 1127
Asp700I GAANNNNTTC 1 cut(s) 936
AspLEI GCGC 1 cut(s) 908
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 1 cut(s) 548
AvaII GGWCC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 645
BbsI GAAGAC 2 cut(s) 64, 153
Bbv12I GWGCWC 1 cut(s) 1230
BbvI GCAGC 7 cut(s) 13, 130, 829, 857, 985, 992, 1064
BccI CCATC 5 cut(s) 88, 197, 394, 602, 898
BciT130I CCWGG 3 cut(s) 647, 746, 994
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 1 cut(s) 548
BcoDI GTCTC 2 cut(s) 255, 1250
BfaI CTAG 3 cut(s) 12, 236, 1232
BfmI CTRYAG 1 cut(s) 843
BfoI RGCGCY 1 cut(s) 909
BfrI CTTAAG 1 cut(s) 515
BisI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
BlsI GCNGC 8 cut(s) 28, 120, 844, 847, 928, 982, 1000, 1054
BmcAI AGTACT 1 cut(s) 381
Bme1390I CCNGG 4 cut(s) 548, 647, 746, 994
Bme18I GGWCC 1 cut(s) 448
BmeRI GACNNNNNGTC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 448
BmrFI CCNGG 4 cut(s) 548, 647, 746, 994
BmsI GCATC 1 cut(s) 1039
BpiI GAAGAC 2 cut(s) 64, 153
BpmI CTGGAG 2 cut(s) 851, 969
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 548
BsaBI GATNNNNATC 1 cut(s) 1155
BsaJI CCNNGG 4 cut(s) 229, 646, 859, 993
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 1010, 1183
Bse1I ACTGG 2 cut(s) 561, 986
Bse3DI GCAATG 1 cut(s) 1109
Bse8I GATNNNNATC 1 cut(s) 1155
BseBI CCWGG 3 cut(s) 647, 746, 994
BseDI CCNNGG 4 cut(s) 229, 646, 859, 993
BseGI GGATG 3 cut(s) 80, 556, 613
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 2 cut(s) 1010, 1183
BseMI GCAATG 1 cut(s) 1109
BseMII CTCAG 1 cut(s) 1257
BseNI ACTGG 2 cut(s) 561, 986
BseRI GAGGAG 2 cut(s) 337, 340
BseXI GCAGC 7 cut(s) 13, 130, 829, 857, 985, 992, 1064
BseYI CCCAGC 1 cut(s) 1177
BsgI GTGCAG 1 cut(s) 81
BshFI GGCC 5 cut(s) 129, 264, 623, 645, 719
BsiHKAI GWGCWC 1 cut(s) 1230
BsiSI CCGG 1 cut(s) 547
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 1010, 1183
BsmAI GTCTC 2 cut(s) 255, 1250
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 5 cut(s) 129, 264, 623, 645, 719
Bsp1286I GDGCHC 1 cut(s) 1230
Bsp143I GATC 3 cut(s) 106, 206, 772
Bsp19I CCATGG 1 cut(s) 859
BspACI CCGC 3 cut(s) 226, 323, 927
BspANI GGCC 5 cut(s) 129, 264, 623, 645, 719
BspCNI CTCAG 1 cut(s) 1258
BspMAI CTGCAG 1 cut(s) 847
BspPI GGATC 1 cut(s) 214
BspTI CTTAAG 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 1109
BsrI ACTGG 2 cut(s) 561, 986
BssECI CCNNGG 4 cut(s) 229, 646, 859, 993
BssMI GATC 3 cut(s) 106, 206, 772
BssT1I CCWWGG 1 cut(s) 859
Bst2UI CCWGG 3 cut(s) 647, 746, 994
Bst4CI ACNGT 5 cut(s) 384, 475, 683, 1008, 1137
BstAFI CTTAAG 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 411, 961
BstDEI CTNAG 3 cut(s) 122, 1084, 1266
BstDSI CCRYGG 1 cut(s) 859
BstF5I GGATG 3 cut(s) 80, 556, 613
BstH2I RGCGCY 1 cut(s) 909
BstHHI GCGC 1 cut(s) 908
BstKTI GATC 3 cut(s) 109, 209, 775
BstMAI GTCTC 2 cut(s) 255, 1250
BstMBI GATC 3 cut(s) 106, 206, 772
BstMWI GCNNNNNNNGC 3 cut(s) 410, 741, 903
BstNI CCWGG 3 cut(s) 647, 746, 994
BstSCI CCNGG 4 cut(s) 546, 645, 744, 992
BstSFI CTRYAG 1 cut(s) 843
BstV1I GCAGC 7 cut(s) 13, 130, 829, 857, 985, 992, 1064
BstV2I GAAGAC 2 cut(s) 64, 153
BstX2I RGATCY 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 1073
BstYI RGATCY 1 cut(s) 206
BsuRI GGCC 5 cut(s) 129, 264, 623, 645, 719
BtgI CCRYGG 1 cut(s) 859
BtgZI GCGATG 1 cut(s) 1257
BtsCI GGATG 3 cut(s) 80, 556, 613
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 3 cut(s) 198, 754, 1004
Cac8I GCNNGC 2 cut(s) 411, 961
CfoI GCGC 1 cut(s) 908
Cfr13I GGNCC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 380, 554
CviAII CATG 5 cut(s) 354, 397, 860, 894, 899
CviQI GTAC 2 cut(s) 380, 554
DdeI CTNAG 3 cut(s) 122, 1084, 1266
DpnI GATC 3 cut(s) 108, 208, 774
DpnII GATC 3 cut(s) 106, 206, 772
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 643
Eam1105I GACNNNNNGTC 1 cut(s) 157
EciI GGCGGA 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 859
Eco147I AGGCCT 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 448
Eco47III AGCGCT 1 cut(s) 907
Eco57I CTGAAG 3 cut(s) 81, 465, 1065
EcoRI GAATTC 2 cut(s) 302, 536
EcoRII CCWGG 3 cut(s) 645, 744, 992
EcoT14I CCWWGG 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 859
FaeI CATG 5 cut(s) 357, 400, 863, 897, 902
FaqI GGGAC 1 cut(s) 62
FatI CATG 5 cut(s) 353, 396, 859, 893, 898
FauI CCCGC 1 cut(s) 316
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 1 cut(s) 772
Fnu4HI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
FokI GGATG 3 cut(s) 67, 563, 620
Fsp4HI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
FspBI CTAG 3 cut(s) 12, 236, 1232
GlaI GCGC 1 cut(s) 907
GluI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
GsaI CCCAGC 1 cut(s) 1181
GsuI CTGGAG 2 cut(s) 851, 969
HaeII RGCGCY 1 cut(s) 909
HaeIII GGCC 5 cut(s) 129, 264, 623, 645, 719
HapII CCGG 1 cut(s) 547
HhaI GCGC 1 cut(s) 908
Hin1II CATG 5 cut(s) 357, 400, 863, 897, 902
Hin6I GCGC 1 cut(s) 906
HinP1I GCGC 1 cut(s) 906
HincII GTYRAC 2 cut(s) 679, 874
HindII GTYRAC 2 cut(s) 679, 874
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 3 cut(s) 439, 833, 1187
HpaII CCGG 1 cut(s) 547
Hpy166II GTNNAC 5 cut(s) 394, 505, 679, 725, 874
Hpy188I TCNGA 7 cut(s) 106, 177, 421, 484, 687, 946, 1035
Hpy188III TCNNGA 5 cut(s) 287, 580, 765, 830, 1191
Hpy8I GTNNAC 5 cut(s) 394, 505, 679, 725, 874
HpyAV CCTTC 1 cut(s) 682
HpyCH4III ACNGT 5 cut(s) 384, 475, 683, 1008, 1137
HpyCH4V TGCA 7 cut(s) 98, 489, 604, 845, 980, 1030, 1052
HpyF10VI GCNNNNNNNGC 3 cut(s) 410, 741, 903
HpyF3I CTNAG 3 cut(s) 122, 1084, 1266
Hsp92II CATG 5 cut(s) 357, 400, 863, 897, 902
HspAI GCGC 1 cut(s) 906
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 3 cut(s) 106, 206, 772
LmnI GCTCC 3 cut(s) 988, 1006, 1013
Lsp1109I GCAGC 7 cut(s) 13, 130, 829, 857, 985, 992, 1064
LweI GCATC 1 cut(s) 1039
MaeI CTAG 3 cut(s) 12, 236, 1232
MaeIII GTNAC 1 cut(s) 988
MalI GATC 3 cut(s) 108, 208, 774
MboI GATC 3 cut(s) 106, 206, 772
MboII GAAGA 7 cut(s) 55, 64, 101, 153, 471, 944, 1094
MfeI CAATTG 1 cut(s) 728
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 1230
MlsI TGGCCA 1 cut(s) 645
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 2 cut(s) 842, 1196
MmeI TCCRAC 3 cut(s) 13, 399, 597
Mox20I TGGCCA 1 cut(s) 645
MroXI GAANNNNTTC 1 cut(s) 936
MscI TGGCCA 1 cut(s) 645
MseI TTAA 5 cut(s) 257, 516, 705, 1142, 1220
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 645
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 1 cut(s) 547
MspR9I CCNGG 4 cut(s) 548, 647, 746, 994
MunI CAATTG 1 cut(s) 728
Mva1269I GAATGC 1 cut(s) 90
MvaI CCWGG 3 cut(s) 647, 746, 994
MwoI GCNNNNNNNGC 3 cut(s) 410, 741, 903
NciI CCSGG 1 cut(s) 548
NcoI CCATGG 1 cut(s) 859
NdeI CATATG 1 cut(s) 804
NdeII GATC 3 cut(s) 106, 206, 772
NlaIII CATG 5 cut(s) 357, 400, 863, 897, 902
NmeAIII GCCGAG 1 cut(s) 254
PceI AGGCCT 1 cut(s) 129
PctI GAATGC 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 936
PfeI GAWTC 1 cut(s) 439
PfoI TCCNGGA 1 cut(s) 546
PkrI GCNGC 8 cut(s) 28, 120, 844, 847, 928, 982, 1000, 1054
PleI GAGTC 2 cut(s) 841, 1195
PpsI GAGTC 2 cut(s) 841, 1195
Psp6I CCWGG 3 cut(s) 645, 744, 992
PspFI CCCAGC 1 cut(s) 1177
PspGI CCWGG 3 cut(s) 645, 744, 992
PspPI GGNCC 1 cut(s) 448
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 2 cut(s) 381, 555
RsaNI GTAC 2 cut(s) 380, 554
RseI CAYNNNNRTG 1 cut(s) 1233
SaqAI TTAA 5 cut(s) 257, 516, 705, 1142, 1220
SatI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
Sau3AI GATC 3 cut(s) 106, 206, 772
Sau96I GGNCC 1 cut(s) 448
ScaI AGTACT 1 cut(s) 381
SchI GAGTC 2 cut(s) 842, 1196
ScrFI CCNGG 4 cut(s) 548, 647, 746, 994
SduI GDGCHC 1 cut(s) 1230
SfaNI GCATC 1 cut(s) 1039
SfcI CTRYAG 1 cut(s) 843
SinI GGWCC 1 cut(s) 448
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 2 cut(s) 287, 515
SmoI CTYRAG 2 cut(s) 287, 515
SseBI AGGCCT 1 cut(s) 129
SsiI CCGC 3 cut(s) 226, 323, 927
SspMI CTAG 3 cut(s) 12, 236, 1232
StuI AGGCCT 1 cut(s) 129
StyD4I CCNGG 4 cut(s) 546, 645, 744, 992
StyI CCWWGG 1 cut(s) 859
TaaI ACNGT 5 cut(s) 384, 475, 683, 1008, 1137
TaqI TCGA 1 cut(s) 1173
TatI WGTACW 2 cut(s) 379, 553
TauI GCSGC 1 cut(s) 929
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 5 cut(s) 257, 516, 705, 1142, 1220
Tru9I TTAA 5 cut(s) 257, 516, 705, 1142, 1220
TscAI CASTG 3 cut(s) 205, 761, 1011
TseI GCWGC 7 cut(s) 26, 118, 842, 845, 980, 998, 1052
TspDTI ATGAA 4 cut(s) 551, 802, 900, 1034
TspRI CASTG 3 cut(s) 205, 761, 1011
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 7 cut(s) 302, 536, 586, 657, 824, 936, 1127
XmnI GAANNNNTTC 1 cut(s) 936
XspI CTAG 3 cut(s) 12, 236, 1232
ZrmI AGTACT 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.