FvH4_6g54080

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003879
Physical Location & Seq
Reverse (-)
39522750 .. 39526586
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54080.t1

Sequence Viewer

Length: 1272 bp
ATGGGGTGCGCTACTTCTGTATATGCCGCTGTTGGAACAAAGAAGAAGTTGACTGTCCCTGAAGTTGTCGTCTTCGTCCCATCCGCTCGCATTCCTGCACAATCTGATCTTCAAAAGCAGCTTAGAGGCCGTATTCCAAAAGATGTTGTTGACAGATTGTCTTCTCTCCGCAATCAGATTGTCTTTGTGGCAGAGGACACCGATGGATCTGCTATACCAGAACTTCGCCGAGGGCTTGAGGAATACTTGTCTCTTTTAATTGGCCTCACTAACAAAGAATATGGTCTTGAGGGATTGATTGAATTCAAGTGGAGAAGTTTAGACGATGGGAAACACCAGGACGCCTCCTCATCATGTGTAGCAAACTCTTGGTTTGAAGTACTGTCTGTTGTTCACATGATGGCTATGCTTGCGTTGTCGGAGGCTGACACAGTGATGATTCCAAAGGACCATTCTGGCTCTAGTATAAGAACTGTCTCTTCAGATTGCAAGAGAGAAGCTGTGGACTTATTGCTTAAGGCAACAGGGTATTTGGAATTCTGTGTCCGGGAAGTACTGGTTCACATACCACCTGAAATCAAGAAAAGTTTTCCGAACGATTTGCAGGATGGTGTGTTGGAGGCCATTTCCTTTCAAACTCTTGGCCAGGGAACTGAAATTCAGCTTGGTTTAGCTGTTGATTGTCAGAAGGCCACTTTATCAGTTAAAAGGAGACTGGCCTGTGAACAATTGAGCTATTTTAGCCAGGCTTATCACTGCTTGTCTGGTCGTGATCACTTCAATAACGGATATGCAAAGAAGCATATGTGGTTCATCAAATGGAAATTTCTCGAGTCAAAGGCTGCAGCATACTACTTCCATGGTTTGATGGTTGACAAGGGTAATGAACCATCATGCCATGTCAAGGCTGTATGTTGTTTTCTTGCCGCAGAAGAAATTCTATCAGAGAGCAAGAAAGCTTGCCTTACCTTTTGCCTTGCAGCTCCAGTTACCAGGGCTGCTCCACTCTGGGGAGCTATGAAGCATTTGCATCAGAAAATCCCTGAAGTTGCAGCAAGGAAATCCCAGATGTATGGCTACCTCTTAGAACAAGAGAAGGTTCTCCAAGCATTGCCTGACCTACCAGAATTTCAACTGTCATTAAGACCTGATGACTATCAACTACCTGAAATTGACCCAGCTTGGGAGTCGGAAAAATGGGAAACTCAAAGCCAGAGCTTGAAAGAGCACCTAAACGATAGCGATGACGATGATGAGACTGAAACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

424

Amino Acids

47.41

Weight (kDa)

5.63

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 86
AciI CCGC 4 cut(s) 27, 84, 169, 927
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 6 cut(s) 302, 536, 657, 824, 936, 1127
AcuI CTGAAG 3 cut(s) 81, 465, 1065
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 2 cut(s) 381, 555
AfiI CCNNNNNNNGG 3 cut(s) 904, 1010, 1183
AflII CTTAAG 1 cut(s) 515
AgsI TTSAA 8 cut(s) 113, 302, 307, 377, 635, 781, 1133, 1222
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 4 cut(s) 336, 645, 744, 992
AjuI GAANNNNNNNTTGG 4 cut(s) 436, 468, 648, 680
Alw21I GWGCWC 1 cut(s) 1230
Alw26I GTCTC 4 cut(s) 255, 481, 706, 1250
AlwI GGATC 1 cut(s) 214
Ama87I CYCGRG 1 cut(s) 830
AoxI GGCC 6 cut(s) 127, 262, 621, 643, 690, 717
ApeKI GCWGC 6 cut(s) 118, 842, 845, 980, 998, 1052
ApoI RAATTY 6 cut(s) 302, 536, 657, 824, 936, 1127
Asp700I GAANNNNTTC 1 cut(s) 936
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 1 cut(s) 548
AvaI CYCGRG 1 cut(s) 830
AvaII GGWCC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 645
BbsI GAAGAC 2 cut(s) 64, 153
Bbv12I GWGCWC 1 cut(s) 1230
BbvI GCAGC 6 cut(s) 130, 829, 857, 985, 992, 1064
BccI CCATC 7 cut(s) 88, 197, 320, 394, 602, 862, 898
BceAI ACGGC 1 cut(s) 114
BcgI CGANNNNNNTGC 4 cut(s) 191, 225, 583, 617
BciT130I CCWGG 4 cut(s) 338, 647, 746, 994
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 1 cut(s) 548
BcoDI GTCTC 4 cut(s) 255, 481, 706, 1250
BfaI CTAG 1 cut(s) 462
BfmI CTRYAG 1 cut(s) 843
BfrI CTTAAG 1 cut(s) 515
BisI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
BlsI GCNGC 8 cut(s) 28, 120, 844, 847, 928, 982, 1000, 1054
BmcAI AGTACT 2 cut(s) 381, 555
Bme1390I CCNGG 5 cut(s) 338, 548, 647, 746, 994
Bme18I GGWCC 1 cut(s) 448
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 830
BmgT120I GGNCC 1 cut(s) 448
BmrFI CCNGG 5 cut(s) 338, 548, 647, 746, 994
BmsI GCATC 1 cut(s) 1039
BpiI GAAGAC 2 cut(s) 64, 153
BpmI CTGGAG 1 cut(s) 969
BpuEI CTTGAG 2 cut(s) 257, 308
BpuMI CCSGG 1 cut(s) 548
BsaBI GATNNNNATC 1 cut(s) 1155
BsaHI GRCGYC 1 cut(s) 342
BsaJI CCNNGG 4 cut(s) 229, 646, 859, 993
Bsc4I CCNNNNNNNGG 3 cut(s) 904, 1010, 1183
Bse1I ACTGG 3 cut(s) 561, 720, 986
Bse3DI GCAATG 1 cut(s) 1109
Bse8I GATNNNNATC 1 cut(s) 1155
BseBI CCWGG 4 cut(s) 338, 647, 746, 994
BseDI CCNNGG 4 cut(s) 229, 646, 859, 993
BseGI GGATG 2 cut(s) 80, 613
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 3 cut(s) 904, 1010, 1183
BseMI GCAATG 1 cut(s) 1109
BseNI ACTGG 3 cut(s) 561, 720, 986
BseRI GAGGAG 1 cut(s) 337
BseXI GCAGC 6 cut(s) 130, 829, 857, 985, 992, 1064
BseYI CCCAGC 1 cut(s) 1177
BsgI GTGCAG 1 cut(s) 81
BshFI GGCC 6 cut(s) 129, 264, 623, 645, 692, 719
BsiHKAI GWGCWC 1 cut(s) 1230
BsiHKCI CYCGRG 1 cut(s) 830
BsiSI CCGG 1 cut(s) 547
BslFI GGGAC 2 cut(s) 41, 62
BslI CCNNNNNNNGG 3 cut(s) 904, 1010, 1183
BsmAI GTCTC 4 cut(s) 255, 481, 706, 1250
BsmFI GGGAC 2 cut(s) 41, 62
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 6 cut(s) 129, 264, 623, 645, 692, 719
BsoBI CYCGRG 1 cut(s) 830
Bsp1286I GDGCHC 1 cut(s) 1230
Bsp143I GATC 3 cut(s) 106, 206, 772
Bsp19I CCATGG 1 cut(s) 859
BspACI CCGC 4 cut(s) 27, 84, 169, 927
BspANI GGCC 6 cut(s) 129, 264, 623, 645, 692, 719
BspMAI CTGCAG 1 cut(s) 847
BspPI GGATC 1 cut(s) 214
BspTI CTTAAG 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 1109
BsrI ACTGG 3 cut(s) 561, 720, 986
BssECI CCNNGG 4 cut(s) 229, 646, 859, 993
BssMI GATC 3 cut(s) 106, 206, 772
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 859
Bst2UI CCWGG 4 cut(s) 338, 647, 746, 994
Bst4CI ACNGT 5 cut(s) 55, 384, 433, 475, 1137
Bst6I CTCTTC 1 cut(s) 484
BstACI GRCGYC 1 cut(s) 342
BstAFI CTTAAG 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 88, 411, 961
BstDEI CTNAG 2 cut(s) 122, 1084
BstDSI CCRYGG 1 cut(s) 859
BstF5I GGATG 2 cut(s) 80, 613
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 3 cut(s) 109, 209, 775
BstMAI GTCTC 4 cut(s) 255, 481, 706, 1250
BstMBI GATC 3 cut(s) 106, 206, 772
BstMWI GCNNNNNNNGC 2 cut(s) 410, 741
BstNI CCWGG 4 cut(s) 338, 647, 746, 994
BstSCI CCNGG 5 cut(s) 336, 546, 645, 744, 992
BstSFI CTRYAG 1 cut(s) 843
BstV1I GCAGC 6 cut(s) 130, 829, 857, 985, 992, 1064
BstV2I GAAGAC 2 cut(s) 64, 153
BstX2I RGATCY 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 1073
BstYI RGATCY 1 cut(s) 206
BsuRI GGCC 6 cut(s) 129, 264, 623, 645, 692, 719
BtgI CCRYGG 1 cut(s) 859
BtgZI GCGATG 1 cut(s) 1257
BtsCI GGATG 2 cut(s) 80, 613
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 2 cut(s) 438, 754
Cac8I GCNNGC 3 cut(s) 88, 411, 961
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 448
CseI GACGC 1 cut(s) 350
Csp6I GTAC 2 cut(s) 380, 554
CviAII CATG 5 cut(s) 354, 397, 860, 894, 899
CviQI GTAC 2 cut(s) 380, 554
DdeI CTNAG 2 cut(s) 122, 1084
DpnI GATC 3 cut(s) 108, 208, 774
DpnII GATC 3 cut(s) 106, 206, 772
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 643
Eam1104I CTCTTC 1 cut(s) 484
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 484
Eco130I CCWWGG 1 cut(s) 859
Eco47I GGWCC 1 cut(s) 448
Eco57I CTGAAG 3 cut(s) 81, 465, 1065
Eco88I CYCGRG 1 cut(s) 830
EcoRI GAATTC 2 cut(s) 302, 536
EcoRII CCWGG 4 cut(s) 336, 645, 744, 992
EcoT14I CCWWGG 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 859
FaeI CATG 5 cut(s) 357, 400, 863, 897, 902
FalI AAGNNNNNCTT 2 cut(s) 948, 980
FaqI GGGAC 2 cut(s) 41, 62
FatI CATG 5 cut(s) 353, 396, 859, 893, 898
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 1 cut(s) 772
Fnu4HI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
FokI GGATG 2 cut(s) 67, 620
Fsp4HI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
FspBI CTAG 1 cut(s) 462
GlaI GCGC 1 cut(s) 10
GluI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
GsaI CCCAGC 1 cut(s) 1181
GsuI CTGGAG 1 cut(s) 969
HaeIII GGCC 6 cut(s) 129, 264, 623, 645, 692, 719
HapII CCGG 1 cut(s) 547
HgaI GACGC 1 cut(s) 350
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 5 cut(s) 357, 400, 863, 897, 902
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 3 cut(s) 51, 151, 874
HindII GTYRAC 3 cut(s) 51, 151, 874
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 3 cut(s) 439, 833, 1187
HpaII CCGG 1 cut(s) 547
Hpy166II GTNNAC 7 cut(s) 51, 151, 394, 505, 562, 725, 874
Hpy188I TCNGA 9 cut(s) 106, 177, 421, 484, 594, 687, 946, 1035, 1192
Hpy188III TCNNGA 4 cut(s) 287, 580, 770, 830
Hpy8I GTNNAC 7 cut(s) 51, 151, 394, 505, 562, 725, 874
HpyAV CCTTC 2 cut(s) 682, 1090
HpyCH4III ACNGT 5 cut(s) 55, 384, 433, 475, 1137
HpyCH4V TGCA 8 cut(s) 98, 489, 604, 794, 845, 980, 1030, 1052
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 741
HpyF3I CTNAG 2 cut(s) 122, 1084
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 5 cut(s) 357, 400, 863, 897, 902
HspAI GCGC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 3 cut(s) 106, 206, 772
LmnI GCTCC 3 cut(s) 988, 1006, 1013
Lsp1109I GCAGC 6 cut(s) 130, 829, 857, 985, 992, 1064
LweI GCATC 1 cut(s) 1039
MaeI CTAG 1 cut(s) 462
MaeIII GTNAC 1 cut(s) 988
MalI GATC 3 cut(s) 108, 208, 774
MbiI CCGCTC 1 cut(s) 86
MboI GATC 3 cut(s) 106, 206, 772
MboII GAAGA 6 cut(s) 55, 64, 101, 153, 471, 944
MfeI CAATTG 1 cut(s) 728
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 1230
MlsI TGGCCA 1 cut(s) 645
MluCI AATT 9 cut(s) 258, 302, 536, 657, 728, 824, 936, 1127, 1170
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 2 cut(s) 842, 1196
MmeI TCCRAC 4 cut(s) 13, 399, 597, 1170
Mox20I TGGCCA 1 cut(s) 645
MroXI GAANNNNTTC 1 cut(s) 936
MscI TGGCCA 1 cut(s) 645
MseI TTAA 4 cut(s) 257, 516, 705, 1142
MslI CAYNNNNRTG 1 cut(s) 434
Msp20I TGGCCA 1 cut(s) 645
MspA1I CMGCKG 1 cut(s) 29
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 1 cut(s) 547
MspR9I CCNGG 5 cut(s) 338, 548, 647, 746, 994
MunI CAATTG 1 cut(s) 728
Mva1269I GAATGC 1 cut(s) 90
MvaI CCWGG 4 cut(s) 338, 647, 746, 994
MwoI GCNNNNNNNGC 2 cut(s) 410, 741
NciI CCSGG 1 cut(s) 548
NcoI CCATGG 1 cut(s) 859
NdeI CATATG 1 cut(s) 804
NdeII GATC 3 cut(s) 106, 206, 772
NlaIII CATG 5 cut(s) 357, 400, 863, 897, 902
NmeAIII GCCGAG 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 830
PctI GAATGC 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 936
PfeI GAWTC 1 cut(s) 439
PfoI TCCNGGA 1 cut(s) 546
PkrI GCNGC 8 cut(s) 28, 120, 844, 847, 928, 982, 1000, 1054
PleI GAGTC 2 cut(s) 841, 1195
PpsI GAGTC 2 cut(s) 841, 1195
Psp6I CCWGG 4 cut(s) 336, 645, 744, 992
PspFI CCCAGC 1 cut(s) 1177
PspGI CCWGG 4 cut(s) 336, 645, 744, 992
PspPI GGNCC 1 cut(s) 448
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 2 cut(s) 381, 555
RsaNI GTAC 2 cut(s) 380, 554
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 4 cut(s) 257, 516, 705, 1142
SatI GCNGC 8 cut(s) 27, 119, 843, 846, 927, 981, 999, 1053
Sau3AI GATC 3 cut(s) 106, 206, 772
Sau96I GGNCC 1 cut(s) 448
ScaI AGTACT 2 cut(s) 381, 555
SchI GAGTC 2 cut(s) 842, 1196
ScrFI CCNGG 5 cut(s) 338, 548, 647, 746, 994
SduI GDGCHC 1 cut(s) 1230
SfaNI GCATC 1 cut(s) 1039
SfcI CTRYAG 1 cut(s) 843
Sfr274I CTCGAG 1 cut(s) 830
SinI GGWCC 1 cut(s) 448
SlaI CTCGAG 1 cut(s) 830
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 4 cut(s) 236, 287, 515, 830
SmoI CTYRAG 4 cut(s) 236, 287, 515, 830
Sse9I AATT 9 cut(s) 258, 302, 536, 657, 728, 824, 936, 1127, 1170
SsiI CCGC 4 cut(s) 27, 84, 169, 927
SspMI CTAG 1 cut(s) 462
StyD4I CCNGG 5 cut(s) 336, 546, 645, 744, 992
StyI CCWWGG 1 cut(s) 859
TaaI ACNGT 5 cut(s) 55, 384, 433, 475, 1137
TaqI TCGA 1 cut(s) 831
TasI AATT 9 cut(s) 258, 302, 536, 657, 728, 824, 936, 1127, 1170
TatI WGTACW 2 cut(s) 379, 553
TauI GCSGC 2 cut(s) 29, 929
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 4 cut(s) 257, 516, 705, 1142
Tru9I TTAA 4 cut(s) 257, 516, 705, 1142
TscAI CASTG 2 cut(s) 438, 761
TseI GCWGC 6 cut(s) 118, 842, 845, 980, 998, 1052
TspDTI ATGAA 3 cut(s) 802, 900, 1034
TspGWI ACGGA 1 cut(s) 801
TspRI CASTG 2 cut(s) 438, 761
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 6 cut(s) 302, 536, 657, 824, 936, 1127
XhoI CTCGAG 1 cut(s) 830
XmnI GAANNNNTTC 1 cut(s) 936
XspI CTAG 1 cut(s) 462
ZrmI AGTACT 2 cut(s) 381, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.