Rroxscaffold_2G00076630

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003879
Physical Location & Seq
Forward (+)
387792 .. 390535
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076630.1

Sequence Viewer

Length: 1278 bp
ATGGGGTGTGCTAGTTCTCTATATGCTGCTGTTGGAACAAAGAAGTTGTCTATCCCTGAAGTTGTCGTCTTCGTCCCATCCACTCGCATTCCTGCACAATCTGATCTTCAAAAGCGGCTTAGAGGCCTAATTCCAAAAGATATTGCTGACAGATTGTCTTCGCTTCGTAATCAGATTGTCTTTGTGGCAGAGGACACTGATGGATCTGCTATACCTGAACTCCGCCGAGGGCTGGAGGAATACTTGTCTCTTTTAATTGGTCTCACTAAAAAAGAATATGGTCTTGAGGGATTGGTTGAATTCAATATAACTGAGTGTGAACTTAATATGATTGAAAAGCAGCAGGAAGCCTCCTCCTCATGTATAGCAAACTCTTGGTTTGAAGTACTGTCTGTTGTTCACATGATGGCTATGCTTGCGTTGTCGGAAGCTGACACATTGATGATTCCAAAGGACCATTCTGGCTCTAGTATAAGGACTGTATCTTCAGATTGCAAGAGGGAAGCTGTGGACTTATTGCTTAAGGCAACAGGGTATTTGGAATTCTGTGTCCGGGATGTACTGGTTCATATACCACCTGAAATCAAGAAAAATTTTCCGAAAGATTTGCAGGATGGTGTGTTGGAGGCCATTTCCTTTCAAACTCTTGGCCAGGGAACTGAAATTCAGCTTGGTTTAGCTGTTGACTGTCAGAAGGCTACTTTATCAGTTAAAAGGAGATTGGCCTGTGAACAATTGAGCTATTTCAGCCAGGCTTATCACTGCTTGTCAGGATATGATCACATCAACAACGGGTATGGAAAGAAGCATATGTGGTTCATCAAATGGAAATTTCTGGAGTCAAAGGCTGCAGCTTACTACTTCCATGGTCTAATGGTTGACAAGGGTAATGAACCATCATGCCATGTTAGCGCTGTATGTTGTTTTCTTGCCGCAGAAGAAATTCTATCAGAGAGCAAGAAAGCTTTCTTTACCTTTTGCCTTGCAGCTCCAGTTACCAGGGCTGCTCCACTGTGGGGAGCTATGAAGCATTTGCATCAGAAAATCCCTGAAGTTGCAGCAAGGAAATCCCAGATGTATGGCTACCTCTTAGAACAAGACAAAGTTCTTCAAGCATTGCCTGACCTACCAGAATTTCAACTGTCATTAAGACCTGATGACTATCAACTACCTGAAATCGACCCAGCTTGGGAGTCGGAAAAATGGGAAACTCAAAGCCAGAGCTTAAAAGAGCACCTAGATGATAGCGATGACAACGATGAGACTGAAACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

425

Amino Acids

47.66

Weight (kDa)

5.19

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 115, 223, 933
AclWI GGATC 1 cut(s) 211
AcoI YGGCCR 1 cut(s) 649
AcsI RAATTY 7 cut(s) 299, 542, 592, 663, 830, 942, 1133
AcuI CTGAAG 3 cut(s) 78, 471, 1071
AfaI GTAC 2 cut(s) 387, 561
AfeI AGCGCT 1 cut(s) 913
AfiI CCNNNNNNNGG 3 cut(s) 232, 1016, 1189
AflII CTTAAG 1 cut(s) 521
AgsI TTSAA 8 cut(s) 110, 299, 304, 335, 383, 641, 1112, 1139
AhdI GACNNNNNGTC 1 cut(s) 154
AjnI CCWGG 3 cut(s) 651, 750, 998
AjuI GAANNNNNNNTTGG 4 cut(s) 442, 474, 654, 686
Alw21I GWGCWC 1 cut(s) 1236
Alw26I GTCTC 3 cut(s) 252, 266, 1256
AlwI GGATC 1 cut(s) 211
Aor51HI AGCGCT 1 cut(s) 913
AoxI GGCC 4 cut(s) 124, 627, 649, 723
ApeKI GCWGC 7 cut(s) 26, 340, 848, 851, 986, 1004, 1058
ApoI RAATTY 7 cut(s) 299, 542, 592, 663, 830, 942, 1133
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
Asp700I GAANNNNTTC 2 cut(s) 942, 965
AspLEI GCGC 1 cut(s) 914
AspS9I GGNCC 1 cut(s) 454
AsuC2I CCSGG 1 cut(s) 554
AvaII GGWCC 1 cut(s) 454
BalI TGGCCA 1 cut(s) 651
BbsI GAAGAC 2 cut(s) 61, 150
Bbv12I GWGCWC 1 cut(s) 1236
BbvI GCAGC 7 cut(s) 13, 352, 835, 863, 991, 998, 1070
BccI CCATC 5 cut(s) 85, 194, 400, 608, 904
BcgI CGANNNNNNTGC 2 cut(s) 589, 623
BciT130I CCWGG 3 cut(s) 653, 752, 1000
BclI TGATCA 1 cut(s) 778
BcnI CCSGG 1 cut(s) 554
BcoDI GTCTC 3 cut(s) 252, 266, 1256
BfaI CTAG 3 cut(s) 12, 468, 1238
BfmI CTRYAG 1 cut(s) 849
BfoI RGCGCY 1 cut(s) 915
BfrI CTTAAG 1 cut(s) 521
BisI GCNGC 9 cut(s) 27, 116, 341, 849, 852, 933, 987, 1005, 1059
BlsI GCNGC 9 cut(s) 28, 117, 342, 850, 853, 934, 988, 1006, 1060
BmcAI AGTACT 1 cut(s) 387
Bme1390I CCNGG 4 cut(s) 554, 653, 752, 1000
Bme18I GGWCC 1 cut(s) 454
BmeRI GACNNNNNGTC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 454
BmrFI CCNGG 4 cut(s) 554, 653, 752, 1000
BmsI GCATC 1 cut(s) 1045
BpiI GAAGAC 2 cut(s) 61, 150
BpmI CTGGAG 3 cut(s) 254, 857, 975
BpuEI CTTGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 554
BsaBI GATNNNNATC 1 cut(s) 1161
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 4 cut(s) 226, 652, 865, 999
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 1016, 1189
Bse1I ACTGG 2 cut(s) 567, 992
Bse3DI GCAATG 1 cut(s) 1115
Bse8I GATNNNNATC 1 cut(s) 1161
BseBI CCWGG 3 cut(s) 653, 752, 1000
BseDI CCNNGG 4 cut(s) 226, 652, 865, 999
BseGI GGATG 3 cut(s) 77, 562, 619
BseJI GATNNNNATC 1 cut(s) 1161
BseLI CCNNNNNNNGG 3 cut(s) 232, 1016, 1189
BseMI GCAATG 1 cut(s) 1115
BseMII CTCAG 2 cut(s) 303, 1263
BseNI ACTGG 2 cut(s) 567, 992
BseRI GAGGAG 2 cut(s) 343, 346
BseXI GCAGC 7 cut(s) 13, 352, 835, 863, 991, 998, 1070
BseYI CCCAGC 1 cut(s) 1183
BsgI GTGCAG 1 cut(s) 78
BshFI GGCC 4 cut(s) 126, 629, 651, 725
BsiHKAI GWGCWC 1 cut(s) 1236
BsiSI CCGG 1 cut(s) 553
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 3 cut(s) 232, 1016, 1189
BsmAI GTCTC 3 cut(s) 252, 266, 1256
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 87
BsnI GGCC 4 cut(s) 126, 629, 651, 725
Bso31I GGTCTC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 1236
Bsp143I GATC 3 cut(s) 103, 203, 778
Bsp19I CCATGG 1 cut(s) 865
BspACI CCGC 3 cut(s) 115, 223, 933
BspANI GGCC 4 cut(s) 126, 629, 651, 725
BspCNI CTCAG 2 cut(s) 304, 1264
BspMAI CTGCAG 1 cut(s) 853
BspPI GGATC 1 cut(s) 211
BspTI CTTAAG 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 1115
BsrI ACTGG 2 cut(s) 567, 992
BssECI CCNNGG 4 cut(s) 226, 652, 865, 999
BssMI GATC 3 cut(s) 103, 203, 778
BssT1I CCWWGG 1 cut(s) 865
Bst2UI CCWGG 3 cut(s) 653, 752, 1000
Bst4CI ACNGT 5 cut(s) 390, 481, 689, 1014, 1143
BstAFI CTTAAG 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 4 cut(s) 119, 312, 1090, 1272
BstDSI CCRYGG 1 cut(s) 865
BstF5I GGATG 3 cut(s) 77, 562, 619
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 1 cut(s) 914
BstKTI GATC 3 cut(s) 106, 206, 781
BstMAI GTCTC 3 cut(s) 252, 266, 1256
BstMBI GATC 3 cut(s) 103, 203, 778
BstMWI GCNNNNNNNGC 3 cut(s) 416, 747, 909
BstNI CCWGG 3 cut(s) 653, 752, 1000
BstSCI CCNGG 4 cut(s) 552, 651, 750, 998
BstSFI CTRYAG 1 cut(s) 849
BstV1I GCAGC 7 cut(s) 13, 352, 835, 863, 991, 998, 1070
BstV2I GAAGAC 2 cut(s) 61, 150
BstX2I RGATCY 1 cut(s) 203
BstXI CCANNNNNNTGG 1 cut(s) 1079
BstYI RGATCY 1 cut(s) 203
BsuRI GGCC 4 cut(s) 126, 629, 651, 725
BtgI CCRYGG 1 cut(s) 865
BtgZI GCGATG 1 cut(s) 1263
BtsCI GGATG 3 cut(s) 77, 562, 619
BtsI GCAGTG 1 cut(s) 760
BtsIMutI CAGTG 3 cut(s) 195, 760, 1010
Cac8I GCNNGC 1 cut(s) 417
CfoI GCGC 1 cut(s) 914
Cfr13I GGNCC 1 cut(s) 454
Csp6I GTAC 2 cut(s) 386, 560
CviAII CATG 5 cut(s) 360, 403, 866, 900, 905
CviQI GTAC 2 cut(s) 386, 560
DdeI CTNAG 4 cut(s) 119, 312, 1090, 1272
DpnI GATC 3 cut(s) 105, 205, 780
DpnII GATC 3 cut(s) 103, 203, 778
DriI GACNNNNNGTC 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 649
Eam1105I GACNNNNNGTC 1 cut(s) 154
EciI GGCGGA 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 865
Eco147I AGGCCT 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 454
Eco47III AGCGCT 1 cut(s) 913
Eco57I CTGAAG 3 cut(s) 78, 471, 1071
EcoRI GAATTC 2 cut(s) 299, 542
EcoRII CCWGG 3 cut(s) 651, 750, 998
EcoT14I CCWWGG 1 cut(s) 865
ErhI CCWWGG 1 cut(s) 865
FaeI CATG 5 cut(s) 363, 406, 869, 903, 908
FaqI GGGAC 1 cut(s) 59
FatI CATG 5 cut(s) 359, 402, 865, 899, 904
FauNDI CATATG 1 cut(s) 810
FbaI TGATCA 1 cut(s) 778
Fnu4HI GCNGC 9 cut(s) 27, 116, 341, 849, 852, 933, 987, 1005, 1059
FokI GGATG 3 cut(s) 64, 569, 626
Fsp4HI GCNGC 9 cut(s) 27, 116, 341, 849, 852, 933, 987, 1005, 1059
FspBI CTAG 3 cut(s) 12, 468, 1238
GlaI GCGC 1 cut(s) 913
GluI GCNGC 9 cut(s) 27, 116, 341, 849, 852, 933, 987, 1005, 1059
GsaI CCCAGC 1 cut(s) 1187
GsuI CTGGAG 3 cut(s) 254, 857, 975
HaeII RGCGCY 1 cut(s) 915
HaeIII GGCC 4 cut(s) 126, 629, 651, 725
HapII CCGG 1 cut(s) 553
HhaI GCGC 1 cut(s) 914
Hin1II CATG 5 cut(s) 363, 406, 869, 903, 908
Hin6I GCGC 1 cut(s) 912
HinP1I GCGC 1 cut(s) 912
HincII GTYRAC 2 cut(s) 685, 880
HindII GTYRAC 2 cut(s) 685, 880
HindIII AAGCTT 1 cut(s) 963
HinfI GANTC 3 cut(s) 445, 839, 1193
HpaII CCGG 1 cut(s) 553
Hpy166II GTNNAC 6 cut(s) 320, 400, 511, 685, 731, 880
Hpy188I TCNGA 9 cut(s) 103, 174, 427, 490, 600, 693, 952, 1041, 1198
Hpy188III TCNNGA 4 cut(s) 284, 586, 771, 836
Hpy8I GTNNAC 6 cut(s) 320, 400, 511, 685, 731, 880
HpyAV CCTTC 1 cut(s) 688
HpyCH4III ACNGT 5 cut(s) 390, 481, 689, 1014, 1143
HpyCH4V TGCA 7 cut(s) 95, 495, 610, 851, 986, 1036, 1058
HpyF10VI GCNNNNNNNGC 3 cut(s) 416, 747, 909
HpyF3I CTNAG 4 cut(s) 119, 312, 1090, 1272
Hsp92II CATG 5 cut(s) 363, 406, 869, 903, 908
HspAI GCGC 1 cut(s) 912
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 3 cut(s) 103, 203, 778
LmnI GCTCC 3 cut(s) 994, 1012, 1019
Lsp1109I GCAGC 7 cut(s) 13, 352, 835, 863, 991, 998, 1070
LweI GCATC 1 cut(s) 1045
MaeI CTAG 3 cut(s) 12, 468, 1238
MaeIII GTNAC 1 cut(s) 994
MalI GATC 3 cut(s) 105, 205, 780
MboI GATC 3 cut(s) 103, 203, 778
MboII GAAGA 6 cut(s) 61, 98, 150, 477, 950, 1100
MfeI CAATTG 1 cut(s) 734
MflI RGATCY 1 cut(s) 203
MhlI GDGCHC 1 cut(s) 1236
MlsI TGGCCA 1 cut(s) 651
MluNI TGGCCA 1 cut(s) 651
MlyI GAGTC 2 cut(s) 848, 1202
MmeI TCCRAC 4 cut(s) 13, 405, 603, 1176
Mox20I TGGCCA 1 cut(s) 651
MroXI GAANNNNTTC 2 cut(s) 942, 965
MscI TGGCCA 1 cut(s) 651
MseI TTAA 6 cut(s) 254, 324, 522, 711, 1148, 1226
MslI CAYNNNNRTG 2 cut(s) 440, 1239
Msp20I TGGCCA 1 cut(s) 651
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 1 cut(s) 553
MspR9I CCNGG 4 cut(s) 554, 653, 752, 1000
MunI CAATTG 1 cut(s) 734
Mva1269I GAATGC 1 cut(s) 87
MvaI CCWGG 3 cut(s) 653, 752, 1000
MwoI GCNNNNNNNGC 3 cut(s) 416, 747, 909
NciI CCSGG 1 cut(s) 554
NcoI CCATGG 1 cut(s) 865
NdeI CATATG 1 cut(s) 810
NdeII GATC 3 cut(s) 103, 203, 778
NlaIII CATG 5 cut(s) 363, 406, 869, 903, 908
NmeAIII GCCGAG 1 cut(s) 251
PceI AGGCCT 1 cut(s) 126
PctI GAATGC 1 cut(s) 87
PdmI GAANNNNTTC 2 cut(s) 942, 965
PfeI GAWTC 1 cut(s) 445
PfoI TCCNGGA 1 cut(s) 552
PkrI GCNGC 9 cut(s) 28, 117, 342, 850, 853, 934, 988, 1006, 1060
PleI GAGTC 2 cut(s) 847, 1201
PpsI GAGTC 2 cut(s) 847, 1201
Psp6I CCWGG 3 cut(s) 651, 750, 998
PspFI CCCAGC 1 cut(s) 1183
PspGI CCWGG 3 cut(s) 651, 750, 998
PspPI GGNCC 1 cut(s) 454
PstI CTGCAG 1 cut(s) 853
PsuI RGATCY 1 cut(s) 203
RsaI GTAC 2 cut(s) 387, 561
RsaNI GTAC 2 cut(s) 386, 560
RseI CAYNNNNRTG 2 cut(s) 440, 1239
SaqAI TTAA 6 cut(s) 254, 324, 522, 711, 1148, 1226
SatI GCNGC 9 cut(s) 27, 116, 341, 849, 852, 933, 987, 1005, 1059
Sau3AI GATC 3 cut(s) 103, 203, 778
Sau96I GGNCC 1 cut(s) 454
ScaI AGTACT 1 cut(s) 387
SchI GAGTC 2 cut(s) 848, 1202
ScrFI CCNGG 4 cut(s) 554, 653, 752, 1000
SduI GDGCHC 1 cut(s) 1236
SfaNI GCATC 1 cut(s) 1045
SfcI CTRYAG 1 cut(s) 849
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 2 cut(s) 440, 1239
SmlI CTYRAG 2 cut(s) 284, 521
SmoI CTYRAG 2 cut(s) 284, 521
SseBI AGGCCT 1 cut(s) 126
SsiI CCGC 3 cut(s) 115, 223, 933
SspMI CTAG 3 cut(s) 12, 468, 1238
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 4 cut(s) 552, 651, 750, 998
StyI CCWWGG 1 cut(s) 865
TaaI ACNGT 5 cut(s) 390, 481, 689, 1014, 1143
TaqI TCGA 1 cut(s) 1179
TatI WGTACW 2 cut(s) 385, 559
TauI GCSGC 2 cut(s) 118, 935
TfiI GAWTC 1 cut(s) 445
Tru1I TTAA 6 cut(s) 254, 324, 522, 711, 1148, 1226
Tru9I TTAA 6 cut(s) 254, 324, 522, 711, 1148, 1226
TscAI CASTG 3 cut(s) 202, 767, 1017
TseI GCWGC 7 cut(s) 26, 340, 848, 851, 986, 1004, 1058
TspDTI ATGAA 4 cut(s) 557, 808, 906, 1040
TspRI CASTG 3 cut(s) 202, 767, 1017
Vha464I CTTAAG 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 7 cut(s) 299, 542, 592, 663, 830, 942, 1133
XmnI GAANNNNTTC 2 cut(s) 942, 965
XspI CTAG 3 cut(s) 12, 468, 1238
ZrmI AGTACT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.