Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011349

Physical Location & Seq

Genomic Coordinates Reverse (-)

4740238 .. 4743539

Gene Structure 3302 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G14700.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGAGGATTGTACGTGCGACGGAGACGTTCTCGGCGGAGCTTAAAGAGTTCATGTGCGCCGCTGTGCAAAGGAGGAAAGACGACGACGGATTCAGAGGCTCTCGTGGCGGTGGTAAATCTAGAAATGCCATGGATCTTGATTCTGACGCCGGAAACAGATTGGTTTGTGTTACTGGTGGTGTTTCTTACCTTGGTCGTGCCATTGTTAAACGTCTTCTTGTTCATGGATACTCCGTTAGAATCGTCGTTGATTGTCCAGAGGACAAAGAGAAAGTGAGTGAGATGGAAGCTGACGCTGAAACGGCGTCGTTTAGCAACATGATTACTTCAGTGGTTTCTCGATTAACAGAGATCGATAGCTTAATTAAAGCGTTTGATGGTTGTGCTGGCGTTTTCCACACTTCTGCGTTTGTTGATCCTGCTGGAGTCTCTGGATATTCGAAATCAATGGCGGAGTTAGAAGCAAAAGTGAGCGAGAGTGTAATCGAAGCGTGTACAAGAACAGCCTCAGTGAGAAAATGTGTCTTCACATCATCTCTCTTAGCCTGCGCTTGGCAAAAAAATCCCTGTAATAGTCTTGACCACTCTGTTATCAATGAAGAAAGCTGGAGCGATGAACAACTCTGCATCGATAATAAGCTTTGGTATGCACTTGGAAAACTAAAAGCCGAGAAAGCTGCGTGGAGAATAGCTGATTCAAAAGGATTAAAGCTTGCAACTATATGTCCTGCTCTTATAACTGGTCCTGACTTCTTCAACCGTAACTCCACTTCAACCTTGGCTTATCTCAAAGGAGCAAAAGAGATGTATAGTAACGGATTATTAGCGACAATGGATGTGAACAGGTTGGCTAAAGCTCACGTATGTTTGTGGGAAGGTTTGGGTAATAAAACTGCGTTTGGTAGATACATTTGTTTCGACACCATTCTCTCTAGAGATGGTGCTGAAAAGCTTGCAAAAGATATCGATGTTCAAATCGAAAAGATTTGCGGTAACAGCAACGATTCAGATGCAAACACAGAAACAGAAGCTTCTTTACAAATATCAGATAAAAAGCTATTAGATCTTATGTCAAGAACTCTACGAAGTTGCTACCATGAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009889 GO:0009890 GO:0009892 GO:0009962 GO:0009964 GO:0009987 GO:0016491 GO:0016627 GO:0016628 GO:0019222 GO:0019438 GO:0019748 GO:0033729 GO:0042742 GO:0043207 GO:0044237 GO:0044249 GO:0044550 GO:0048519 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0071704 GO:0080090 GO:0098542 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

3Beta_HSD Epimerase

Protein Analysis

368

Amino Acids

40.27

Weight (kDa)

6.06

Isoelectric Point (pI)

33.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	55 - 267	1.8e-12	NAD dependent epimerase/dehydratase family
3Beta_HSD	PF01073	57 - 248	6.3e-12	3-beta hydroxysteroid dehydrogenase/isomerase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011349)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14700 AT5G14700
fragaria_vesca	FvH4_6g50850
malus_domestica	MD09G1031700.v1.1 MD17G1033400.v1.1
prunus_persica	Prupe.3G286200_v2.0.a1
pyrus_communis	pycom111g02510 pycom17g03060
rosa_chinensis	RchiOBHm_Chr2g0172231
rosa_laevigata	RLG00000022098
rosa_multiflora	Rmu_co8477553.1_g000001 Rmu_sc0024549.1_g000006 Rmu_sc0025855.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080040
rosa_rugosa	Rorug02G0558000
rosa_samantha	Rh2AG633100 Rh2BG646700 Rh2CG613600 Rh2DG661500
rosa_wichuraiana	Rw2G052410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	737
AciI	CCGC	5 cut(s)	35, 60, 108, 452, 990
AclWI	GGATC	2 cut(s)	141, 410
AcuI	CTGAAG	1 cut(s)	312
AcyI	GRCGYC	2 cut(s)	147, 305
AfaI	GTAC	2 cut(s)	12, 496
AfiI	CCNNNNNNNGG	1 cut(s)	552
AgsI	TTSAA	4 cut(s)	699, 757, 774, 974
Alw26I	GTCTC	2 cut(s)	17, 433
AlwI	GGATC	2 cut(s)	141, 410
ApeKI	GCWGC	1 cut(s)	677
AspLEI	GCGC	2 cut(s)	59, 551
AspS9I	GGNCC	1 cut(s)	743
AsuII	TTCGAA	1 cut(s)	440
AvaII	GGWCC	1 cut(s)	743
BaeI	ACNNNNGTAYC	2 cut(s)	898, 931
BarI	GAAGNNNNNNTAC	4 cut(s)	754, 786, 1020, 1052
BauI	CACGAG	1 cut(s)	102
BbsI	GAAGAC	2 cut(s)	206, 517
BbvI	GCAGC	1 cut(s)	664
BccI	CCATC	3 cut(s)	277, 371, 932
BceAI	ACGGC	1 cut(s)	318
BcgI	CGANNNNNNTGC	4 cut(s)	659, 693, 992, 1026
BciVI	GTATCC	1 cut(s)	221
BcoDI	GTCTC	2 cut(s)	17, 433
BfaI	CTAG	2 cut(s)	120, 933
BfuI	GTATCC	1 cut(s)	221
BglII	AGATCT	1 cut(s)	1063
BisI	GCNGC	2 cut(s)	60, 678
BlsI	GCNGC	2 cut(s)	61, 679
Bme18I	GGWCC	1 cut(s)	743
BmgT120I	GGNCC	1 cut(s)	743
BmsI	GCATC	2 cut(s)	636, 1000
BpiI	GAAGAC	2 cut(s)	206, 517
BplI	GAGNNNNNCTC	2 cut(s)	14, 46
BpmI	CTGGAG	2 cut(s)	444, 628
Bpu14I	TTCGAA	1 cut(s)	440
Bsa29I	ATCGAT	3 cut(s)	354, 630, 966
BsaAI	YACGTR	2 cut(s)	14, 862
BsaHI	GRCGYC	2 cut(s)	147, 305
BsaJI	CCNNGG	3 cut(s)	129, 190, 777
BsaXI	ACNNNNNCTCC	2 cut(s)	749, 779
Bsc4I	CCNNNNNNNGG	1 cut(s)	552
Bse1I	ACTGG	2 cut(s)	178, 745
BseCI	ATCGAT	3 cut(s)	354, 630, 966
BseDI	CCNNGG	3 cut(s)	129, 190, 777
BseGI	GGATG	1 cut(s)	841
BseLI	CCNNNNNNNGG	1 cut(s)	552
BseMII	CTCAG	1 cut(s)	522
BseNI	ACTGG	2 cut(s)	178, 745
BseXI	GCAGC	1 cut(s)	664
BshVI	ATCGAT	3 cut(s)	354, 630, 966
BsiSI	CCGG	1 cut(s)	150
BslI	CCNNNNNNNGG	1 cut(s)	552
BsmAI	GTCTC	2 cut(s)	17, 433
BsmBI	CGTCTC	1 cut(s)	17
Bsp119I	TTCGAA	1 cut(s)	440
Bsp1407I	TGTACA	1 cut(s)	494
Bsp143I	GATC	4 cut(s)	133, 351, 415, 1063
Bsp19I	CCATGG	1 cut(s)	129
BspACI	CCGC	5 cut(s)	35, 60, 108, 452, 990
BspCNI	CTCAG	1 cut(s)	521
BspDI	ATCGAT	3 cut(s)	354, 630, 966
BspPI	GGATC	2 cut(s)	141, 410
BspT104I	TTCGAA	1 cut(s)	440
BsrGI	TGTACA	1 cut(s)	494
BsrI	ACTGG	2 cut(s)	178, 745
BssECI	CCNNGG	3 cut(s)	129, 190, 777
BssMI	GATC	4 cut(s)	133, 351, 415, 1063
BssNI	GRCGYC	2 cut(s)	147, 305
BssSI	CACGAG	1 cut(s)	102
BssT1I	CCWWGG	3 cut(s)	129, 190, 777
Bst2BI	CACGAG	1 cut(s)	102
Bst4CI	ACNGT	1 cut(s)	761
BstACI	GRCGYC	2 cut(s)	147, 305
BstAUI	TGTACA	1 cut(s)	494
BstBAI	YACGTR	2 cut(s)	14, 862
BstBI	TTCGAA	1 cut(s)	440
BstC8I	GCNNGC	4 cut(s)	388, 547, 714, 954
BstDEI	CTNAG	2 cut(s)	508, 541
BstDSI	CCRYGG	1 cut(s)	129
BstF5I	GGATG	1 cut(s)	841
BstHHI	GCGC	2 cut(s)	59, 551
BstKTI	GATC	4 cut(s)	136, 354, 418, 1066
BstMAI	GTCTC	2 cut(s)	17, 433
BstMBI	GATC	4 cut(s)	133, 351, 415, 1063
BstMWI	GCNNNNNNNGC	4 cut(s)	105, 302, 674, 996
BstV1I	GCAGC	1 cut(s)	664
BstV2I	GAAGAC	2 cut(s)	206, 517
BstX2I	RGATCY	2 cut(s)	133, 1063
BstYI	RGATCY	2 cut(s)	133, 1063
Bsu15I	ATCGAT	3 cut(s)	354, 630, 966
BsuI	GTATCC	1 cut(s)	221
BsuTUI	ATCGAT	3 cut(s)	354, 630, 966
BtgI	CCRYGG	1 cut(s)	129
BtgZI	GCGATG	1 cut(s)	627
BtsCI	GGATG	1 cut(s)	841
BtsIMutI	CAGTG	2 cut(s)	336, 516
Cac8I	GCNNGC	4 cut(s)	388, 547, 714, 954
CfoI	GCGC	2 cut(s)	59, 551
Cfr13I	GGNCC	1 cut(s)	743
ClaI	ATCGAT	3 cut(s)	354, 630, 966
CseI	GACGC	3 cut(s)	155, 294, 302
Csp6I	GTAC	2 cut(s)	11, 495
CviAII	CATG	5 cut(s)	52, 130, 224, 319, 1097
CviQI	GTAC	2 cut(s)	11, 495
DdeI	CTNAG	2 cut(s)	508, 541
DpnI	GATC	4 cut(s)	135, 353, 417, 1065
DpnII	GATC	4 cut(s)	133, 351, 415, 1063
EciI	GGCGGA	2 cut(s)	50, 467
Eco130I	CCWWGG	3 cut(s)	129, 190, 777
Eco32I	GATATC	1 cut(s)	964
Eco47I	GGWCC	1 cut(s)	743
Eco57I	CTGAAG	1 cut(s)	312
EcoRV	GATATC	1 cut(s)	964
EcoT14I	CCWWGG	3 cut(s)	129, 190, 777
ErhI	CCWWGG	3 cut(s)	129, 190, 777
Esp3I	CGTCTC	1 cut(s)	17
FaeI	CATG	5 cut(s)	55, 133, 227, 322, 1100
FatI	CATG	5 cut(s)	51, 129, 223, 318, 1096
Fnu4HI	GCNGC	2 cut(s)	60, 678
FokI	GGATG	1 cut(s)	848
Fsp4HI	GCNGC	2 cut(s)	60, 678
FspBI	CTAG	2 cut(s)	120, 933
GlaI	GCGC	2 cut(s)	58, 550
GluI	GCNGC	2 cut(s)	60, 678
GsuI	CTGGAG	2 cut(s)	444, 628
HapII	CCGG	1 cut(s)	150
HgaI	GACGC	3 cut(s)	155, 294, 302
HhaI	GCGC	2 cut(s)	59, 551
Hin1I	GRCGYC	2 cut(s)	147, 305
Hin1II	CATG	5 cut(s)	55, 133, 227, 322, 1100
Hin6I	GCGC	2 cut(s)	57, 549
HinP1I	GCGC	2 cut(s)	57, 549
HindIII	AAGCTT	4 cut(s)	638, 710, 950, 1029
HinfI	GANTC	6 cut(s)	90, 140, 240, 426, 695, 1004
HpaII	CCGG	1 cut(s)	150
Hpy166II	GTNNAC	2 cut(s)	495, 841
Hpy188I	TCNGA	4 cut(s)	95, 145, 1009, 1048
Hpy188III	TCNNGA	9 cut(s)	120, 137, 257, 339, 432, 578, 746, 933, 1074
Hpy8I	GTNNAC	2 cut(s)	495, 841
Hpy99I	CGWCG	5 cut(s)	22, 86, 89, 248, 310
HpyAV	CCTTC	1 cut(s)	869
HpyCH4III	ACNGT	1 cut(s)	761
HpyCH4IV	ACGT	4 cut(s)	13, 26, 211, 861
HpyCH4V	TGCA	6 cut(s)	67, 627, 650, 716, 956, 1013
HpyF10VI	GCNNNNNNNGC	4 cut(s)	105, 302, 674, 996
HpyF3I	CTNAG	2 cut(s)	508, 541
HpySE526I	ACGT	4 cut(s)	13, 26, 211, 861
Hsp92I	GRCGYC	2 cut(s)	147, 305
Hsp92II	CATG	5 cut(s)	55, 133, 227, 322, 1100
HspAI	GCGC	2 cut(s)	57, 549
Kzo9I	GATC	4 cut(s)	133, 351, 415, 1063
LmnI	GCTCC	3 cut(s)	37, 609, 794
Lsp1109I	GCAGC	1 cut(s)	664
LweI	GCATC	2 cut(s)	636, 1000
MaeI	CTAG	2 cut(s)	120, 933
MaeII	ACGT	4 cut(s)	13, 26, 211, 861
MaeIII	GTNAC	4 cut(s)	169, 761, 812, 992
MalI	GATC	4 cut(s)	135, 353, 417, 1065
MboI	GATC	4 cut(s)	133, 351, 415, 1063
MboII	GAAGA	4 cut(s)	206, 517, 611, 745
MflI	RGATCY	2 cut(s)	133, 1063
MluCI	AATT	1 cut(s)	363
MlyI	GAGTC	1 cut(s)	435
MnlI	CCTC	4 cut(s)	66, 89, 253, 517
MseI	TTAA	6 cut(s)	42, 207, 344, 362, 366, 707
MspA1I	CMGCKG	1 cut(s)	62
MspI	CCGG	1 cut(s)	150
MwoI	GCNNNNNNNGC	4 cut(s)	105, 302, 674, 996
NcoI	CCATGG	1 cut(s)	129
NdeII	GATC	4 cut(s)	133, 351, 415, 1063
NlaIII	CATG	5 cut(s)	55, 133, 227, 322, 1100
NmeAIII	GCCGAG	2 cut(s)	11, 694
NspV	TTCGAA	1 cut(s)	440
PacI	TTAATTAA	1 cut(s)	366
PfeI	GAWTC	5 cut(s)	90, 140, 240, 695, 1004
PkrI	GCNGC	2 cut(s)	61, 679
PleI	GAGTC	1 cut(s)	434
PpsI	GAGTC	1 cut(s)	434
Ppu21I	YACGTR	2 cut(s)	14, 862
PsiI	TTATAA	1 cut(s)	737
PspPI	GGNCC	1 cut(s)	743
PsuI	RGATCY	2 cut(s)	133, 1063
RsaI	GTAC	2 cut(s)	12, 496
RsaNI	GTAC	2 cut(s)	11, 495
SaqAI	TTAA	6 cut(s)	42, 207, 344, 362, 366, 707
SatI	GCNGC	2 cut(s)	60, 678
Sau3AI	GATC	4 cut(s)	133, 351, 415, 1063
Sau96I	GGNCC	1 cut(s)	743
SchI	GAGTC	1 cut(s)	435
SfaNI	GCATC	2 cut(s)	636, 1000
SfuI	TTCGAA	1 cut(s)	440
SinI	GGWCC	1 cut(s)	743
Sse9I	AATT	1 cut(s)	363
SsiI	CCGC	5 cut(s)	35, 60, 108, 452, 990
SspMI	CTAG	2 cut(s)	120, 933
StyI	CCWWGG	3 cut(s)	129, 190, 777
TaaI	ACNGT	1 cut(s)	761
TaiI	ACGT	4 cut(s)	16, 29, 214, 864
TaqI	TCGA	8 cut(s)	340, 354, 440, 486, 630, 918, 966, 978
TasI	AATT	1 cut(s)	363
TatI	WGTACW	1 cut(s)	494
TauI	GCSGC	1 cut(s)	62
TfiI	GAWTC	5 cut(s)	90, 140, 240, 695, 1004
Tru1I	TTAA	6 cut(s)	42, 207, 344, 362, 366, 707
Tru9I	TTAA	6 cut(s)	42, 207, 344, 362, 366, 707
TscAI	CASTG	2 cut(s)	336, 516
TseI	GCWGC	1 cut(s)	677
TspDTI	ATGAA	4 cut(s)	40, 212, 612, 630
TspGWI	ACGGA	4 cut(s)	35, 102, 223, 831
TspRI	CASTG	2 cut(s)	336, 516
VpaK11BI	GGWCC	1 cut(s)	743
XbaI	TCTAGA	2 cut(s)	119, 932
XcmI	CCANNNNNNNNNTGG	1 cut(s)	775
XspI	CTAG	2 cut(s)	120, 933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.