Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0011349

Physical Location & Seq

Genomic Coordinates Reverse (-)

1902530 .. 1907380

Gene Structure 4851 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1031700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1095 bp

ATGGTCGAAATGGGGATTGTGGGTTTGGAAGAGAGGGTGAACATCGAGGTGCAGGAGTTCTGCCGTGCGTTGGTGGCCTCCGCCGGCCTCCACCGCAGAAAAGACGACCACCAGCAACCCGACTGCAAGCGTTTCGACTCGAGGGGTGATTATGAACAAGACATGTTGGTGTGTGTCACTAGCGGCGTTTCCTTCTTAGGCCTTGCCATCGTTGACCGTCTCCTCCACCGTGGCTACTCCGTCCGCCTCATCGTTGACAACCAAGAAGATGCAGAAATAATGAGGGAGATGAGGACAAGCGGCACCAACAGCGATCGCATATCTGCAGTTATTGCGAAGCTAACCGACACCGACGTTGTTGAAAGCTTGTCACACGCGTTCCATGGCTGTCGTGGCGTTTTCCACACCTCTGCATTTGTTGACCCCGCTGGCCTCTCTGGTTACACTAAATCCATGGCTGAGATAGAAGTGAAGGCAAGTGAGAATGTGATGAAGGCATGTGCAATGACGCCATCTGTGAGAAAATGTGTGCTGACTTCTTCACTTTTAGCCTGCGTTTGGCAAGATAGCTCCCAGAATTTGAACTTCTCCCGTGTGATTAACCATAACCACTGGAGCAGTGAGTCACTCTGCATAGATAAAAAGCTCTGGTATGCATTGGGCAAGCTGAGGGCAGAGAAAGCTGCATGGAAAATAGCCGAGGAAAGGGGGTTGAAGTTGACCACCATCTGCCCGGCTCTCATTACCGGCCCTGAAATTTCCACTAGAAATCCAACCGCAACTCTTGCATATCTCAAGGCGCAAGAAATGTACCAAAGTGGAGTGCTAGCCACAGTGGATATAACAAGACTGGCAGAGGCACATGTAGGGGTGTTTGAGGCAATGAACAAGGCAGCATTTGGCAGATACATCTGGTTCGATCGAGTCATTGACGGAGAAGAAGAGGCCGAGAAGTTAGCAGAGGCGACGGGCATGTCGAAGAACAAGATCCTGGGCAATGGCGGCTCTAACGTTGTCCAGAATCGATTCGAATTGTCGAACAAGAAGCTTACAAATCTTTTGACGGGAAGAGTACATTGCTCTTACCATTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009889 GO:0009890 GO:0009892 GO:0009962 GO:0009964 GO:0009987 GO:0016491 GO:0016627 GO:0016628 GO:0019222 GO:0019438 GO:0019748 GO:0033729 GO:0042742 GO:0043207 GO:0044237 GO:0044249 GO:0044550 GO:0048519 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0071704 GO:0080090 GO:0098542 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

3Beta_HSD Epimerase

Protein Analysis

365

Amino Acids

40.35

Weight (kDa)

6.61

Isoelectric Point (pI)

32.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	58 - 261	1.6e-08	NAD dependent epimerase/dehydratase family
3Beta_HSD	PF01073	59 - 274	4.7e-11	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10	PF13460	66 - 252	2.8e-06	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011349)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14700 AT5G14700
fragaria_vesca	FvH4_6g50850
malus_domestica	MD09G1031700.v1.1 MD17G1033400.v1.1
prunus_persica	Prupe.3G286200_v2.0.a1
pyrus_communis	pycom111g02510 pycom17g03060
rosa_chinensis	RchiOBHm_Chr2g0172231
rosa_laevigata	RLG00000022098
rosa_multiflora	Rmu_co8477553.1_g000001 Rmu_sc0024549.1_g000006 Rmu_sc0025855.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080040
rosa_rugosa	Rorug02G0558000
rosa_samantha	Rh2AG633100 Rh2BG646700 Rh2CG613600 Rh2DG661500
rosa_wichuraiana	Rw2G052410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	973
AccB1I	GGYRCC	1 cut(s)	302
AccII	CGCG	1 cut(s)	377
AciI	CCGC	8 cut(s)	81, 94, 183, 244, 300, 426, 777, 1002
AclI	AACGTT	1 cut(s)	1011
AclWI	GGATC	1 cut(s)	982
AcsI	RAATTY	2 cut(s)	577, 756
AcyI	GRCGYC	1 cut(s)	509
AfaI	GTAC	2 cut(s)	812, 1074
AfiI	CCNNNNNNNGG	3 cut(s)	70, 558, 705
AflIII	ACRYGT	3 cut(s)	162, 375, 862
AgsI	TTSAA	3 cut(s)	362, 583, 715
AjnI	CCWGG	1 cut(s)	990
AluBI	AGCT	7 cut(s)	340, 366, 570, 646, 667, 683, 1048
AluI	AGCT	7 cut(s)	340, 366, 570, 646, 667, 683, 1048
Alw26I	GTCTC	1 cut(s)	224
AlwI	GGATC	1 cut(s)	982
Ama87I	CYCGRG	1 cut(s)	139
AoxI	GGCC	6 cut(s)	75, 85, 199, 430, 748, 945
ApeKI	GCWGC	2 cut(s)	683, 893
ApoI	RAATTY	2 cut(s)	577, 756
AsiSI	GCGATCGC	1 cut(s)	316
Asp700I	GAANNNNTTC	1 cut(s)	1025
AspLEI	GCGC	1 cut(s)	802
AspS9I	GGNCC	1 cut(s)	749
AsuC2I	CCSGG	1 cut(s)	734
AsuHPI	GGTGA	2 cut(s)	49, 158
AsuII	TTCGAA	1 cut(s)	1029
AsuNHI	GCTAGC	1 cut(s)	826
AvaI	CYCGRG	1 cut(s)	139
BanI	GGYRCC	1 cut(s)	302
BbvCI	CCTCAGC	1 cut(s)	668
BbvI	GCAGC	2 cut(s)	670, 905
BccI	CCATC	3 cut(s)	215, 520, 734
BceAI	ACGGC	1 cut(s)	48
BcgI	CGANNNNNNTGC	2 cut(s)	115, 149
BciT130I	CCWGG	1 cut(s)	992
BcnI	CCSGG	1 cut(s)	734
BcoDI	GTCTC	1 cut(s)	224
BfaI	CTAG	3 cut(s)	180, 765, 827
BfmI	CTRYAG	1 cut(s)	324
BisI	GCNGC	5 cut(s)	184, 301, 684, 894, 1003
BlsI	GCNGC	5 cut(s)	185, 302, 685, 895, 1004
Bme1390I	CCNGG	2 cut(s)	734, 992
BmeT110I	CYCGRG	1 cut(s)	139
BmgT120I	GGNCC	1 cut(s)	749
BmiI	GGNNCC	1 cut(s)	304
BmrFI	CCNGG	2 cut(s)	734, 992
BmsI	GCATC	1 cut(s)	259
BmtI	GCTAGC	1 cut(s)	830
BpmI	CTGGAG	1 cut(s)	634
Bpu10I	CCTNAGC	1 cut(s)	668
Bpu14I	TTCGAA	1 cut(s)	1029
BpuEI	CTTGAG	1 cut(s)	779
BpuMI	CCSGG	1 cut(s)	734
Bsa29I	ATCGAT	1 cut(s)	1024
BsaHI	GRCGYC	1 cut(s)	509
BsaJI	CCNNGG	5 cut(s)	229, 382, 453, 699, 991
Bsc4I	CCNNNNNNNGG	3 cut(s)	70, 558, 705
Bse118I	RCCGGY	2 cut(s)	83, 746
Bse1I	ACTGG	2 cut(s)	617, 855
Bse3DI	GCAATG	4 cut(s)	510, 888, 1003, 1075
BseBI	CCWGG	1 cut(s)	992
BseCI	ATCGAT	1 cut(s)	1024
BseDI	CCNNGG	5 cut(s)	229, 382, 453, 699, 991
BseLI	CCNNNNNNNGG	3 cut(s)	70, 558, 705
BseMI	GCAATG	4 cut(s)	510, 888, 1003, 1075
BseMII	CTCAG	2 cut(s)	450, 659
BseNI	ACTGG	2 cut(s)	617, 855
BseRI	GAGGAG	1 cut(s)	212
BseXI	GCAGC	2 cut(s)	670, 905
BsgI	GTGCAG	1 cut(s)	71
Bsh1236I	CGCG	1 cut(s)	377
Bsh1285I	CGRYCG	2 cut(s)	316, 922
BshFI	GGCC	6 cut(s)	77, 87, 201, 432, 750, 947
BshNI	GGYRCC	1 cut(s)	302
BshVI	ATCGAT	1 cut(s)	1024
BsiEI	CGRYCG	2 cut(s)	316, 922
BsiHKCI	CYCGRG	1 cut(s)	139
BsiSI	CCGG	3 cut(s)	84, 734, 747
BslI	CCNNNNNNNGG	3 cut(s)	70, 558, 705
BsmAI	GTCTC	1 cut(s)	224
BsmBI	CGTCTC	1 cut(s)	224
BsnI	GGCC	6 cut(s)	77, 87, 201, 432, 750, 947
BsoBI	CYCGRG	1 cut(s)	139
Bsp119I	TTCGAA	1 cut(s)	1029
Bsp143I	GATC	3 cut(s)	313, 919, 987
Bsp19I	CCATGG	2 cut(s)	382, 453
BspACI	CCGC	8 cut(s)	81, 94, 183, 244, 300, 426, 777, 1002
BspANI	GGCC	6 cut(s)	77, 87, 201, 432, 750, 947
BspCNI	CTCAG	2 cut(s)	451, 660
BspDI	ATCGAT	1 cut(s)	1024
BspFNI	CGCG	1 cut(s)	377
BspLI	GGNNCC	1 cut(s)	304
BspMAI	CTGCAG	1 cut(s)	328
BspOI	GCTAGC	1 cut(s)	830
BspPI	GGATC	1 cut(s)	982
BspT104I	TTCGAA	1 cut(s)	1029
BspT107I	GGYRCC	1 cut(s)	302
BsrDI	GCAATG	4 cut(s)	510, 888, 1003, 1075
BsrFI	RCCGGY	2 cut(s)	83, 746
BsrI	ACTGG	2 cut(s)	617, 855
BssAI	RCCGGY	2 cut(s)	83, 746
BssECI	CCNNGG	5 cut(s)	229, 382, 453, 699, 991
BssMI	GATC	3 cut(s)	313, 919, 987
BssNI	GRCGYC	1 cut(s)	509
BssT1I	CCWWGG	2 cut(s)	382, 453
Bst2UI	CCWGG	1 cut(s)	992
Bst4CI	ACNGT	3 cut(s)	218, 230, 835
Bst6I	CTCTTC	3 cut(s)	24, 936, 1063
BstACI	GRCGYC	1 cut(s)	509
BstAPI	GCANNNNNTGC	2 cut(s)	332, 785
BstBI	TTCGAA	1 cut(s)	1029
BstC8I	GCNNGC	6 cut(s)	85, 128, 430, 553, 665, 828
BstDEI	CTNAG	3 cut(s)	196, 459, 668
BstDSI	CCRYGG	3 cut(s)	229, 382, 453
BstFNI	CGCG	1 cut(s)	377
BstHHI	GCGC	1 cut(s)	802
BstKTI	GATC	3 cut(s)	316, 922, 990
BstMAI	GTCTC	1 cut(s)	224
BstMBI	GATC	3 cut(s)	313, 919, 987
BstMCI	CGRYCG	2 cut(s)	316, 922
BstMWI	GCNNNNNNNGC	8 cut(s)	74, 93, 309, 332, 393, 680, 785, 1002
BstNI	CCWGG	1 cut(s)	992
BstNSI	RCATGY	4 cut(s)	166, 501, 866, 976
BstSCI	CCNGG	2 cut(s)	732, 990
BstSFI	CTRYAG	1 cut(s)	324
BstUI	CGCG	1 cut(s)	377
BstV1I	GCAGC	2 cut(s)	670, 905
BstX2I	RGATCY	1 cut(s)	987
BstYI	RGATCY	1 cut(s)	987
Bsu15I	ATCGAT	1 cut(s)	1024
BsuRI	GGCC	6 cut(s)	77, 87, 201, 432, 750, 947
BsuTUI	ATCGAT	1 cut(s)	1024
BtgI	CCRYGG	3 cut(s)	229, 382, 453
BtsI	GCAGTG	1 cut(s)	625
BtsIMutI	CAGTG	3 cut(s)	610, 625, 840
Cac8I	GCNNGC	6 cut(s)	85, 128, 430, 553, 665, 828
CfoI	GCGC	1 cut(s)	802
Cfr10I	RCCGGY	2 cut(s)	83, 746
Cfr13I	GGNCC	1 cut(s)	749
ClaI	ATCGAT	1 cut(s)	1024
CseI	GACGC	1 cut(s)	517
Csp6I	GTAC	2 cut(s)	811, 1073
CviAII	CATG	7 cut(s)	163, 383, 454, 498, 687, 863, 973
CviQI	GTAC	2 cut(s)	811, 1073
DdeI	CTNAG	3 cut(s)	196, 459, 668
DpnI	GATC	3 cut(s)	315, 921, 989
DpnII	GATC	3 cut(s)	313, 919, 987
DrdI	GACNNNNNNGTC	1 cut(s)	973
DseDI	GACNNNNNNGTC	1 cut(s)	973
Eam1104I	CTCTTC	3 cut(s)	24, 936, 1063
EarI	CTCTTC	3 cut(s)	24, 936, 1063
EciI	GGCGGA	2 cut(s)	70, 233
Eco130I	CCWWGG	2 cut(s)	382, 453
Eco147I	AGGCCT	1 cut(s)	201
Eco88I	CYCGRG	1 cut(s)	139
EcoRII	CCWGG	1 cut(s)	990
EcoT14I	CCWWGG	2 cut(s)	382, 453
EcoT22I	ATGCAT	1 cut(s)	658
ErhI	CCWWGG	2 cut(s)	382, 453
Esp3I	CGTCTC	1 cut(s)	224
FaeI	CATG	7 cut(s)	166, 386, 457, 501, 690, 866, 976
FatI	CATG	7 cut(s)	162, 382, 453, 497, 686, 862, 972
FauI	CCCGC	1 cut(s)	433
Fnu4HI	GCNGC	5 cut(s)	184, 301, 684, 894, 1003
Fsp4HI	GCNGC	5 cut(s)	184, 301, 684, 894, 1003
FspBI	CTAG	3 cut(s)	180, 765, 827
GlaI	GCGC	1 cut(s)	801
GluI	GCNGC	5 cut(s)	184, 301, 684, 894, 1003
GsuI	CTGGAG	1 cut(s)	634
HaeIII	GGCC	6 cut(s)	77, 87, 201, 432, 750, 947
HapII	CCGG	3 cut(s)	84, 734, 747
HgaI	GACGC	1 cut(s)	517
HhaI	GCGC	1 cut(s)	802
Hin1I	GRCGYC	1 cut(s)	509
Hin1II	CATG	7 cut(s)	166, 386, 457, 501, 690, 866, 976
Hin6I	GCGC	1 cut(s)	800
HinP1I	GCGC	1 cut(s)	800
HincII	GTYRAC	4 cut(s)	214, 256, 421, 720
HindII	GTYRAC	4 cut(s)	214, 256, 421, 720
HindIII	AAGCTT	2 cut(s)	364, 1046
HinfI	GANTC	5 cut(s)	137, 623, 924, 1021, 1026
HpaII	CCGG	3 cut(s)	84, 734, 747
HphI	GGTGA	2 cut(s)	49, 158
Hpy166II	GTNNAC	5 cut(s)	40, 214, 256, 421, 720
Hpy188III	TCNNGA	1 cut(s)	1018
Hpy8I	GTNNAC	5 cut(s)	40, 214, 256, 421, 720
Hpy99I	CGWCG	2 cut(s)	356, 970
HpyAV	CCTTC	3 cut(s)	202, 466, 487
HpyCH4III	ACNGT	3 cut(s)	218, 230, 835
HpyCH4IV	ACGT	2 cut(s)	354, 1011
HpyF10VI	GCNNNNNNNGC	8 cut(s)	74, 93, 309, 332, 393, 680, 785, 1002
HpyF3I	CTNAG	3 cut(s)	196, 459, 668
HpySE526I	ACGT	2 cut(s)	354, 1011
Hsp92I	GRCGYC	1 cut(s)	509
Hsp92II	CATG	7 cut(s)	166, 386, 457, 501, 690, 866, 976
HspAI	GCGC	1 cut(s)	800
KroI	GCCGGC	1 cut(s)	83
KroNI	GCCGGC	1 cut(s)	85
Kzo9I	GATC	3 cut(s)	313, 919, 987
LmnI	GCTCC	2 cut(s)	575, 615
Lsp1109I	GCAGC	2 cut(s)	670, 905
LweI	GCATC	1 cut(s)	259
MaeI	CTAG	3 cut(s)	180, 765, 827
MaeII	ACGT	2 cut(s)	354, 1011
MaeIII	GTNAC	4 cut(s)	175, 369, 440, 624
MalI	GATC	3 cut(s)	315, 921, 989
MboI	GATC	3 cut(s)	313, 919, 987
MboII	GAAGA	7 cut(s)	41, 278, 531, 950, 953, 991, 1080
MflI	RGATCY	1 cut(s)	987
MluCI	AATT	3 cut(s)	577, 756, 1031
MluI	ACGCGT	1 cut(s)	375
MlyI	GAGTC	3 cut(s)	131, 632, 933
MmeI	TCCRAC	1 cut(s)	797
Mph1103I	ATGCAT	1 cut(s)	658
MroNI	GCCGGC	1 cut(s)	83
MroXI	GAANNNNTTC	1 cut(s)	1025
MseI	TTAA	1 cut(s)	600
MslI	CAYNNNNRTG	2 cut(s)	47, 167
MspA1I	CMGCKG	1 cut(s)	428
MspI	CCGG	3 cut(s)	84, 734, 747
MspR9I	CCNGG	2 cut(s)	734, 992
MvaI	CCWGG	1 cut(s)	992
MvnI	CGCG	1 cut(s)	377
MwoI	GCNNNNNNNGC	8 cut(s)	74, 93, 309, 332, 393, 680, 785, 1002
NaeI	GCCGGC	1 cut(s)	85
NciI	CCSGG	1 cut(s)	734
NcoI	CCATGG	2 cut(s)	382, 453
NdeII	GATC	3 cut(s)	313, 919, 987
NgoMIV	GCCGGC	1 cut(s)	83
NheI	GCTAGC	1 cut(s)	826
NlaIII	CATG	7 cut(s)	166, 386, 457, 501, 690, 866, 976
NlaIV	GGNNCC	1 cut(s)	304
NmeAIII	GCCGAG	2 cut(s)	724, 973
NmuCI	GTSAC	3 cut(s)	175, 369, 624
NsiI	ATGCAT	1 cut(s)	658
NspI	RCATGY	4 cut(s)	166, 501, 866, 976
NspV	TTCGAA	1 cut(s)	1029
PaeR7I	CTCGAG	1 cut(s)	139
PceI	AGGCCT	1 cut(s)	201
PciI	ACATGT	2 cut(s)	162, 862
PcsI	WCGNNNNNNNCGW	1 cut(s)	974
PdiI	GCCGGC	1 cut(s)	85
PdmI	GAANNNNTTC	1 cut(s)	1025
PfeI	GAWTC	2 cut(s)	1021, 1026
PkrI	GCNGC	5 cut(s)	185, 302, 685, 895, 1004
Ple19I	CGATCG	2 cut(s)	316, 922
PleI	GAGTC	3 cut(s)	131, 631, 932
PpsI	GAGTC	3 cut(s)	131, 631, 932
PscI	ACATGT	2 cut(s)	162, 862
Psp1406I	AACGTT	1 cut(s)	1011
Psp6I	CCWGG	1 cut(s)	990
PspGI	CCWGG	1 cut(s)	990
PspN4I	GGNNCC	1 cut(s)	304
PspPI	GGNCC	1 cut(s)	749
PspXI	VCTCGAGB	1 cut(s)	139
PstI	CTGCAG	1 cut(s)	328
PsuI	RGATCY	1 cut(s)	987
PvuI	CGATCG	2 cut(s)	316, 922
RgaI	GCGATCGC	1 cut(s)	316
RsaI	GTAC	2 cut(s)	812, 1074
RsaNI	GTAC	2 cut(s)	811, 1073
RseI	CAYNNNNRTG	2 cut(s)	47, 167
SaqAI	TTAA	1 cut(s)	600
SatI	GCNGC	5 cut(s)	184, 301, 684, 894, 1003
Sau3AI	GATC	3 cut(s)	313, 919, 987
Sau96I	GGNCC	1 cut(s)	749
SchI	GAGTC	3 cut(s)	131, 632, 933
ScrFI	CCNGG	2 cut(s)	734, 992
SfaAI	GCGATCGC	1 cut(s)	316
SfaNI	GCATC	1 cut(s)	259
SfcI	CTRYAG	1 cut(s)	324
Sfr274I	CTCGAG	1 cut(s)	139
SfuI	TTCGAA	1 cut(s)	1029
SgfI	GCGATCGC	1 cut(s)	316
SlaI	CTCGAG	1 cut(s)	139
SmiMI	CAYNNNNRTG	2 cut(s)	47, 167
SmlI	CTYRAG	2 cut(s)	139, 794
SmoI	CTYRAG	2 cut(s)	139, 794
Sse9I	AATT	3 cut(s)	577, 756, 1031
SseBI	AGGCCT	1 cut(s)	201
SsiI	CCGC	8 cut(s)	81, 94, 183, 244, 300, 426, 777, 1002
SspMI	CTAG	3 cut(s)	180, 765, 827
StuI	AGGCCT	1 cut(s)	201
StyD4I	CCNGG	2 cut(s)	732, 990
StyI	CCWWGG	2 cut(s)	382, 453
TaaI	ACNGT	3 cut(s)	218, 230, 835
TaiI	ACGT	2 cut(s)	357, 1014
TasI	AATT	3 cut(s)	577, 756, 1031
TatI	WGTACW	1 cut(s)	1072
TauI	GCSGC	3 cut(s)	186, 303, 1005
TfiI	GAWTC	2 cut(s)	1021, 1026
Tru1I	TTAA	1 cut(s)	600
Tru9I	TTAA	1 cut(s)	600
TscAI	CASTG	3 cut(s)	617, 625, 840
TseFI	GTSAC	3 cut(s)	175, 369, 624
TseI	GCWGC	2 cut(s)	683, 893
Tsp45I	GTSAC	3 cut(s)	175, 369, 624
TspDTI	ATGAA	4 cut(s)	168, 506, 899, 1080
TspGWI	ACGGA	2 cut(s)	229, 948
TspRI	CASTG	3 cut(s)	617, 625, 840
XapI	RAATTY	2 cut(s)	577, 756
XceI	RCATGY	4 cut(s)	166, 501, 866, 976
XcmI	CCANNNNNNNNNTGG	1 cut(s)	389
XhoI	CTCGAG	1 cut(s)	139
XmnI	GAANNNNTTC	1 cut(s)	1025
XspI	CTAG	3 cut(s)	180, 765, 827
Zsp2I	ATGCAT	1 cut(s)	658

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.