Rroxscaffold_2G00080040

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011349
Physical Location & Seq
Reverse (-)
2924000 .. 2926234
2235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00080040.1

Sequence Viewer

Length: 987 bp
ATGGGGATTGCTCGTTTACAAGAGAGGGAAGAAGATCACGAGCTATTACAAGAGCTCCGCCGCATGTTGGTCTCCTCCGCGGGCCAAGACAAACCACACCTTCCACAAAAGCCCTTCTCATCTAAAAGCGCTTATGAAGATGACTCGGAAGAGAAGCTCGTCTGTGTCACAAGCGGCGTTTCCTTCTTGGGCCTCGCGGTCGTCAACCGCCTCCTCCTCCGCGGCTACTCCGTCCGCATCATCGTTAATAACTCCGTGGACGTTGAAAAACTGAGGGAGATGACGACGGCAAACGTTAACGTCACCGTAGTTATGGCGAAGCTAGCAGAAGTTGAAAGCTTATCACAAGCATTTCAGGGCTGTCGTGGCGTCTTCCACACCTCCGGATTTACGGATCCTGCTGGCCTTTCTGGGTACACTAAATCCATGGCTGATATCGAAGTGAAGGCCAGTGAGAATGTGATGAGGGCATGTGCAGTGACACCTTCAGTAAGAAAATGTGTGCTCACTTCTTCACTTTTAGCCTGTGTATGGCAAGAGCAAGACATTTCACAAAATGACCTCTCCACAATCCCTGTTATCAACCATGACTGCTGGAGCAATCAGTCACTCTGCATAGAAAAAAAGCTGTGGTATGCTTTGGGGAAACTTAGAGCAGAGAAAGCTGCATGGACATTAGCCAAAGAAACTGGGTTAAAGCTCACTACAATCTGCCCAGGTCTTATTACTGGCCCTGAATTTTGCTCTAGAAACCCAACAGCAACAATTGCATATCTCAAAGGTGCCCAAGAGATGTACCAATGTGGCGTGCTAGCAACCGTGGATGTGATGAGACTGGCAGAAGTTCATGTAAGTGTATATAAGGCGATGAACAAATCAGCATTTGGTAGATACATTTGCTTTGATCAAGTCGTTGAAACAGAGAAAGAGGCAGAGAAGCTAGCAGGGGGAGACCAGTATGTCAAAGAACAAGATATGCGGAAATGA

Protein Analysis

328

Amino Acids

36.29

Weight (kDa)

6.27

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 51 - 129 7.6e-08 NmrA-like family
Epimerase PF01370 55 - 255 1.6e-07 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 56 - 184 9.7e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 243 7.4e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14700 AT5G14700
fragaria_vesca FvH4_6g50850
malus_domestica MD09G1031700.v1.1 MD17G1033400.v1.1
prunus_persica Prupe.3G286200_v2.0.a1
pyrus_communis pycom111g02510 pycom17g03060
rosa_chinensis RchiOBHm_Chr2g0172231
rosa_laevigata RLG00000022098
rosa_multiflora Rmu_co8477553.1_g000001 Rmu_sc0024549.1_g000006 Rmu_sc0025855.1_g000001
rosa_roxburghii Rroxscaffold_2G00080040
rosa_rugosa Rorug02G0558000
rosa_samantha Rh2AG633100 Rh2BG646700 Rh2CG613600 Rh2DG661500
rosa_wichuraiana Rw2G052410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 959
AccB1I GGYRCC 1 cut(s) 782
AccII CGCG 3 cut(s) 80, 197, 222
AccIII TCCGGA 1 cut(s) 383
AclI AACGTT 1 cut(s) 294
AclWI GGATC 2 cut(s) 389, 402
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 369
AfaI GTAC 2 cut(s) 416, 797
AfeI AGCGCT 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 67, 531
AgsI TTSAA 3 cut(s) 266, 335, 917
AjnI CCWGG 1 cut(s) 715
AluBI AGCT 9 cut(s) 43, 55, 157, 322, 339, 628, 665, 700, 940
AluI AGCT 9 cut(s) 43, 55, 157, 322, 339, 628, 665, 700, 940
Alw21I GWGCWC 2 cut(s) 57, 507
Alw26I GTCTC 3 cut(s) 76, 826, 945
AlwI GGATC 2 cut(s) 389, 402
Aor13HI TCCGGA 1 cut(s) 383
Aor51HI AGCGCT 1 cut(s) 130
AoxI GGCC 5 cut(s) 82, 190, 403, 447, 730
ApeKI GCWGC 1 cut(s) 665
ApoI RAATTY 1 cut(s) 737
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 82, 190, 731
AsuHPI GGTGA 1 cut(s) 295
AsuNHI GCTAGC 3 cut(s) 322, 811, 940
BaeGI GKGCMC 1 cut(s) 787
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 57
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 364
Bbv12I GWGCWC 2 cut(s) 57, 507
BbvI GCAGC 1 cut(s) 652
BceAI ACGGC 1 cut(s) 303
BciT130I CCWGG 1 cut(s) 717
BclI TGATCA 1 cut(s) 904
BcoDI GTCTC 3 cut(s) 76, 826, 945
BfaI CTAG 4 cut(s) 323, 747, 812, 941
BfoI RGCGCY 1 cut(s) 132
BisI GCNGC 4 cut(s) 61, 175, 223, 666
BlsI GCNGC 4 cut(s) 62, 176, 224, 667
Bme1390I CCNGG 1 cut(s) 717
BmgT120I GGNCC 3 cut(s) 82, 190, 731
BmiI GGNNCC 2 cut(s) 396, 784
BmrFI CCNGG 1 cut(s) 717
BmrI ACTGGG 1 cut(s) 699
BmsI GCATC 1 cut(s) 246
BmtI GCTAGC 3 cut(s) 326, 815, 944
BmuI ACTGGG 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 616
BsaHI GRCGYC 1 cut(s) 369
BsaI GGTCTC 2 cut(s) 76, 945
BsaJI CCNNGG 6 cut(s) 78, 220, 255, 426, 715, 819
BsaWI WCCGGW 1 cut(s) 383
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 531
Bse1I ACTGG 5 cut(s) 450, 694, 733, 840, 955
BseAI TCCGGA 1 cut(s) 383
BseBI CCWGG 1 cut(s) 717
BseDI CCNNGG 6 cut(s) 78, 220, 255, 426, 715, 819
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 2 cut(s) 67, 531
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 5 cut(s) 450, 694, 733, 840, 955
BseRI GAGGAG 3 cut(s) 64, 203, 206
BseSI GKGCMC 1 cut(s) 787
BseXI GCAGC 1 cut(s) 652
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 3 cut(s) 80, 197, 222
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 5 cut(s) 84, 192, 405, 449, 732
BshNI GGYRCC 1 cut(s) 782
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 57, 507
BsiSI CCGG 1 cut(s) 384
BslI CCNNNNNNNGG 2 cut(s) 67, 531
BsmAI GTCTC 3 cut(s) 76, 826, 945
BsnI GGCC 5 cut(s) 84, 192, 405, 449, 732
Bso31I GGTCTC 2 cut(s) 76, 945
Bsp1286I GDGCHC 3 cut(s) 57, 507, 787
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 3 cut(s) 34, 394, 904
Bsp19I CCATGG 1 cut(s) 426
BspANI GGCC 5 cut(s) 84, 192, 405, 449, 732
BspCNI CTCAG 1 cut(s) 264
BspEI TCCGGA 1 cut(s) 383
BspFNI CGCG 3 cut(s) 80, 197, 222
BspLI GGNNCC 2 cut(s) 396, 784
BspOI GCTAGC 3 cut(s) 326, 815, 944
BspPI GGATC 2 cut(s) 389, 402
BspT107I GGYRCC 1 cut(s) 782
BspTNI GGTCTC 2 cut(s) 76, 945
BsrI ACTGG 5 cut(s) 450, 694, 733, 840, 955
BssECI CCNNGG 6 cut(s) 78, 220, 255, 426, 715, 819
BssMI GATC 3 cut(s) 34, 394, 904
BssNI GRCGYC 1 cut(s) 369
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 426
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 2 cut(s) 307, 820
Bst6I CTCTTC 1 cut(s) 144
BstACI GRCGYC 1 cut(s) 369
BstAPI GCANNNNNTGC 1 cut(s) 767
BstC8I GCNNGC 6 cut(s) 82, 324, 403, 809, 813, 942
BstDEI CTNAG 2 cut(s) 272, 650
BstDSI CCRYGG 5 cut(s) 78, 220, 255, 426, 819
BstF5I GGATG 1 cut(s) 829
BstFNI CGCG 3 cut(s) 80, 197, 222
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 3 cut(s) 37, 397, 907
BstMAI GTCTC 3 cut(s) 76, 826, 945
BstMBI GATC 3 cut(s) 34, 394, 904
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 4 cut(s) 323, 366, 662, 767
BstNI CCWGG 1 cut(s) 717
BstNSI RCATGY 2 cut(s) 67, 474
BstSCI CCNGG 1 cut(s) 715
BstSLI GKGCMC 1 cut(s) 787
BstUI CGCG 3 cut(s) 80, 197, 222
BstV1I GCAGC 1 cut(s) 652
BstV2I GAAGAC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 5 cut(s) 84, 192, 405, 449, 732
BtgI CCRYGG 5 cut(s) 78, 220, 255, 426, 819
BtgZI GCGATG 1 cut(s) 881
BtsCI GGATG 1 cut(s) 829
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 457, 483
Cac8I GCNNGC 6 cut(s) 82, 324, 403, 809, 813, 942
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 82, 190, 731
Cfr42I CCGCGG 2 cut(s) 81, 223
CseI GACGC 1 cut(s) 358
Csp6I GTAC 2 cut(s) 415, 796
CviAII CATG 6 cut(s) 64, 427, 471, 587, 669, 848
CviQI GTAC 2 cut(s) 415, 796
DdeI CTNAG 2 cut(s) 272, 650
DpnI GATC 3 cut(s) 36, 396, 906
DpnII GATC 3 cut(s) 34, 394, 904
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
EciI GGCGGA 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 426
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 2 cut(s) 76, 945
Eco32I GATATC 1 cut(s) 436
Eco47III AGCGCT 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 715
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 6 cut(s) 67, 430, 474, 590, 672, 851
FatI CATG 6 cut(s) 63, 426, 470, 586, 668, 847
FauI CCCGC 1 cut(s) 73
FbaI TGATCA 1 cut(s) 904
Fnu4HI GCNGC 4 cut(s) 61, 175, 223, 666
FokI GGATG 1 cut(s) 836
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 4 cut(s) 61, 175, 223, 666
FspBI CTAG 4 cut(s) 323, 747, 812, 941
GlaI GCGC 1 cut(s) 130
GluI GCNGC 4 cut(s) 61, 175, 223, 666
GsuI CTGGAG 1 cut(s) 616
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 5 cut(s) 84, 192, 405, 449, 732
HapII CCGG 1 cut(s) 384
HgaI GACGC 1 cut(s) 358
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 369
Hin1II CATG 6 cut(s) 67, 430, 474, 590, 672, 851
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 2 cut(s) 205, 298
HindII GTYRAC 2 cut(s) 205, 298
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 1 cut(s) 143
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 1 cut(s) 384
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 5 cut(s) 17, 205, 259, 298, 417
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 3 cut(s) 38, 384, 747
Hpy8I GTNNAC 5 cut(s) 17, 205, 259, 298, 417
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 5 cut(s) 110, 124, 193, 439, 495
HpyCH4III ACNGT 2 cut(s) 307, 820
HpyCH4IV ACGT 3 cut(s) 261, 294, 300
HpyCH4V TGCA 4 cut(s) 476, 615, 668, 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 366, 662, 767
HpyF3I CTNAG 2 cut(s) 272, 650
HpySE526I ACGT 3 cut(s) 261, 294, 300
Hsp92I GRCGYC 1 cut(s) 369
Hsp92II CATG 6 cut(s) 67, 430, 474, 590, 672, 851
HspAI GCGC 1 cut(s) 129
Kpn2I TCCGGA 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 904
KspAI GTTAAC 1 cut(s) 298
KspI CCGCGG 2 cut(s) 81, 223
Kzo9I GATC 3 cut(s) 34, 394, 904
LmnI GCTCC 2 cut(s) 60, 597
Lsp1109I GCAGC 1 cut(s) 652
LweI GCATC 1 cut(s) 246
MaeI CTAG 4 cut(s) 323, 747, 812, 941
MaeII ACGT 3 cut(s) 261, 294, 300
MaeIII GTNAC 4 cut(s) 166, 301, 478, 606
MalI GATC 3 cut(s) 36, 396, 906
MboI GATC 3 cut(s) 34, 394, 904
MboII GAAGA 6 cut(s) 41, 44, 149, 161, 364, 504
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 3 cut(s) 57, 507, 787
MluCI AATT 2 cut(s) 737, 765
MlyI GAGTC 1 cut(s) 137
MroI TCCGGA 1 cut(s) 383
MseI TTAA 3 cut(s) 246, 297, 695
MslI CAYNNNNRTG 1 cut(s) 852
MspA1I CMGCKG 2 cut(s) 80, 222
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 1 cut(s) 717
MunI CAATTG 1 cut(s) 765
MvaI CCWGG 1 cut(s) 717
MvnI CGCG 3 cut(s) 80, 197, 222
MwoI GCNNNNNNNGC 4 cut(s) 323, 366, 662, 767
NcoI CCATGG 1 cut(s) 426
NdeII GATC 3 cut(s) 34, 394, 904
NheI GCTAGC 3 cut(s) 322, 811, 940
NlaIII CATG 6 cut(s) 67, 430, 474, 590, 672, 851
NlaIV GGNNCC 2 cut(s) 396, 784
NmuCI GTSAC 4 cut(s) 166, 301, 478, 606
NspI RCATGY 2 cut(s) 67, 474
PkrI GCNGC 4 cut(s) 62, 176, 224, 667
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 57
Psp1406I AACGTT 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 715
PspGI CCWGG 1 cut(s) 715
PspN4I GGNNCC 2 cut(s) 396, 784
PspPI GGNCC 3 cut(s) 82, 190, 731
PsuI RGATCY 1 cut(s) 394
RsaI GTAC 2 cut(s) 416, 797
RsaNI GTAC 2 cut(s) 415, 796
RseI CAYNNNNRTG 1 cut(s) 852
SacI GAGCTC 1 cut(s) 57
SacII CCGCGG 2 cut(s) 81, 223
SaqAI TTAA 3 cut(s) 246, 297, 695
SatI GCNGC 4 cut(s) 61, 175, 223, 666
Sau3AI GATC 3 cut(s) 34, 394, 904
Sau96I GGNCC 3 cut(s) 82, 190, 731
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 1 cut(s) 717
SduI GDGCHC 3 cut(s) 57, 507, 787
SfaNI GCATC 1 cut(s) 246
Sfr303I CCGCGG 2 cut(s) 81, 223
SgrBI CCGCGG 2 cut(s) 81, 223
SmiMI CAYNNNNRTG 1 cut(s) 852
Sse9I AATT 2 cut(s) 737, 765
SspMI CTAG 4 cut(s) 323, 747, 812, 941
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 715
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 2 cut(s) 307, 820
TaiI ACGT 3 cut(s) 264, 297, 303
TaqI TCGA 1 cut(s) 438
TasI AATT 2 cut(s) 737, 765
TauI GCSGC 3 cut(s) 63, 177, 225
Tru1I TTAA 3 cut(s) 246, 297, 695
Tru9I TTAA 3 cut(s) 246, 297, 695
TscAI CASTG 2 cut(s) 457, 483
TseFI GTSAC 4 cut(s) 166, 301, 478, 606
TseI GCWGC 1 cut(s) 665
Tsp45I GTSAC 4 cut(s) 166, 301, 478, 606
TspDTI ATGAA 3 cut(s) 150, 836, 884
TspGWI ACGGA 3 cut(s) 220, 244, 407
TspRI CASTG 2 cut(s) 457, 483
XapI RAATTY 1 cut(s) 737
XbaI TCTAGA 1 cut(s) 746
XceI RCATGY 2 cut(s) 67, 474
XspI CTAG 4 cut(s) 323, 747, 812, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.