Rh2DG661500

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011349
Physical Location & Seq
Forward (+)
88777270 .. 88781966
4697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG661500.1

Sequence Viewer

Length: 903 bp
ATGGGGATTGCTCGTTTACAAGAGAGGGAAGAAGATCACGAGCTATTACAAGAGCTCCGCCGCATGTTGGTCTCCTCCGCTGGCCAAGACAAACCACACCTTCCACAAAAGTGCTTCTCATCCAAAAGCGCTTATGAAGATGACTCGGAAGAGAAGCTAGTCTGCGTCACAAGCGGCGTTTCCTTCTTGGGCCTCGCCGTCGTCAACCGCCTCCTCCTCCGCGGCTACTCCGTCCGCATCATCGTTAATAACTCCTTGGACGTTGAGAAACTGAGGGAGATGACGACGGCGAACGTTAACGTCACTGTAGTTATGGCGAAGCTAGCAGAAGTTGAAAGCTTATCACAAGCATTTCAGGGCTGTCGTGGCGTCTTCCACACCTCTGGATTTACCGATCCTGCTGGCCTTTCTGGGTACACTAAATCCATGGCTGATATCGAAGTGAAGGCCAGTGCGAATGTGATGAGGGCATGTGCAGTGACACCTTCAGTAAGAAAATGCGTGCTCACTTCTTCACTTTTAGCCTGTGTATGGCAAGAGCAAGACATTTCACAAAATGACCTCTCCACAATCCCTGTTATCAACCATGACTGCTGGAGCAATGAGTCACTCTGCATAGAAAAAAAGCTGTGGTATGCTTTGGGGAAGCTTAGAGCAGAGAAAGCTGCATGGACATTAGCCAAAGAAACTGGGTTAAAGCTCACTACAATCTGCCCAGGTCTTATTACTGGCCCTGAATTTTTCTCTAGAAACCCAACAGCAACAATTGCATATCTCAAAGGGAGATTGTTGAGTAAGACAATCCCTCAATTGCAGCAGAACAGTAAGCCAAAATCTGCTCATCATGTTGGACTCATAACCAGATTCAAGCTCGCTTTTAACCTAGACAATGAACTCGTTTAG

Protein Analysis

300

Amino Acids

33.12

Weight (kDa)

8.52

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 51 - 129 9.1e-08 NmrA-like family
Epimerase PF01370 55 - 265 6.2e-08 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 56 - 184 2.9e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 242 6.8e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14700 AT5G14700
fragaria_vesca FvH4_6g50850
malus_domestica MD09G1031700.v1.1 MD17G1033400.v1.1
prunus_persica Prupe.3G286200_v2.0.a1
pyrus_communis pycom111g02510 pycom17g03060
rosa_chinensis RchiOBHm_Chr2g0172231
rosa_laevigata RLG00000022098
rosa_multiflora Rmu_co8477553.1_g000001 Rmu_sc0024549.1_g000006 Rmu_sc0025855.1_g000001
rosa_roxburghii Rroxscaffold_2G00080040
rosa_rugosa Rorug02G0558000
rosa_samantha Rh2AG633100 Rh2BG646700 Rh2CG613600 Rh2DG661500
rosa_wichuraiana Rw2G052410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 222
AciI CCGC 8 cut(s) 58, 61, 78, 174, 208, 220, 222, 235
AclI AACGTT 1 cut(s) 294
AclWI GGATC 1 cut(s) 389
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 369
AfaI GTAC 1 cut(s) 416
AfeI AGCGCT 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 67, 531
AgsI TTSAA 2 cut(s) 335, 868
AjnI CCWGG 1 cut(s) 715
AleI CACNNNNGTG 1 cut(s) 109
Alw21I GWGCWC 2 cut(s) 57, 507
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 130
AoxI GGCC 5 cut(s) 82, 190, 403, 447, 730
ApeKI GCWGC 2 cut(s) 665, 814
ApoI RAATTY 1 cut(s) 737
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 190, 731
AsuNHI GCTAGC 1 cut(s) 322
BalI TGGCCA 1 cut(s) 84
BanII GRGCYC 1 cut(s) 57
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 364
Bbv12I GWGCWC 2 cut(s) 57, 507
BbvI GCAGC 2 cut(s) 652, 826
BceAI ACGGC 2 cut(s) 182, 303
BciT130I CCWGG 1 cut(s) 717
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 4 cut(s) 158, 323, 747, 884
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 132
BisI GCNGC 5 cut(s) 61, 175, 223, 666, 815
BlsI GCNGC 5 cut(s) 62, 176, 224, 667, 816
Bme1390I CCNGG 1 cut(s) 717
BmgT120I GGNCC 2 cut(s) 190, 731
BmrFI CCNGG 1 cut(s) 717
BmrI ACTGGG 1 cut(s) 699
BmsI GCATC 1 cut(s) 246
BmtI GCTAGC 1 cut(s) 326
BmuI ACTGGG 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 616
BsaHI GRCGYC 1 cut(s) 369
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 4 cut(s) 220, 255, 426, 715
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 531
Bse1I ACTGG 3 cut(s) 450, 694, 733
Bse3DI GCAATG 1 cut(s) 607
BseBI CCWGG 1 cut(s) 717
BseDI CCNNGG 4 cut(s) 220, 255, 426, 715
BseGI GGATG 1 cut(s) 119
BseLI CCNNNNNNNGG 2 cut(s) 67, 531
BseMI GCAATG 1 cut(s) 607
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 3 cut(s) 450, 694, 733
BseRI GAGGAG 3 cut(s) 64, 203, 206
BseXI GCAGC 2 cut(s) 652, 826
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 5 cut(s) 84, 192, 405, 449, 732
BsiHKAI GWGCWC 2 cut(s) 57, 507
BslI CCNNNNNNNGG 2 cut(s) 67, 531
BsmAI GTCTC 1 cut(s) 76
BsnI GGCC 5 cut(s) 84, 192, 405, 449, 732
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 57, 507
Bsp143I GATC 2 cut(s) 34, 394
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 8 cut(s) 58, 61, 78, 174, 208, 220, 222, 235
BspANI GGCC 5 cut(s) 84, 192, 405, 449, 732
BspCNI CTCAG 1 cut(s) 264
BspFNI CGCG 1 cut(s) 222
BspOI GCTAGC 1 cut(s) 326
BspPI GGATC 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 607
BsrI ACTGG 3 cut(s) 450, 694, 733
BssECI CCNNGG 4 cut(s) 220, 255, 426, 715
BssMI GATC 2 cut(s) 34, 394
BssNI GRCGYC 1 cut(s) 369
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 255, 426
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 2 cut(s) 307, 824
Bst6I CTCTTC 1 cut(s) 144
BstACI GRCGYC 1 cut(s) 369
BstAPI GCANNNNNTGC 1 cut(s) 767
BstC8I GCNNGC 5 cut(s) 82, 324, 403, 503, 873
BstDEI CTNAG 2 cut(s) 272, 650
BstDSI CCRYGG 2 cut(s) 220, 426
BstF5I GGATG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 37, 397
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 34, 394
BstMWI GCNNNNNNNGC 5 cut(s) 171, 323, 366, 662, 767
BstNI CCWGG 1 cut(s) 717
BstNSI RCATGY 2 cut(s) 67, 474
BstSCI CCNGG 1 cut(s) 715
BstSFI CTRYAG 1 cut(s) 306
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 2 cut(s) 652, 826
BstV2I GAAGAC 1 cut(s) 364
BstXI CCANNNNNNTGG 1 cut(s) 383
BsuRI GGCC 5 cut(s) 84, 192, 405, 449, 732
BtgI CCRYGG 2 cut(s) 220, 426
BtsCI GGATG 1 cut(s) 119
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 3 cut(s) 303, 457, 483
Cac8I GCNNGC 5 cut(s) 82, 324, 403, 503, 873
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 190, 731
Cfr42I CCGCGG 1 cut(s) 223
CseI GACGC 2 cut(s) 154, 358
Csp6I GTAC 1 cut(s) 415
CviAII CATG 6 cut(s) 64, 427, 471, 587, 669, 845
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 2 cut(s) 272, 650
DpnI GATC 2 cut(s) 36, 396
DpnII GATC 2 cut(s) 34, 394
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
EciI GGCGGA 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 2 cut(s) 255, 426
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 76
Eco32I GATATC 1 cut(s) 436
Eco47III AGCGCT 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 715
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 2 cut(s) 255, 426
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 255, 426
FaeI CATG 6 cut(s) 67, 430, 474, 590, 672, 848
FatI CATG 6 cut(s) 63, 426, 470, 586, 668, 844
Fnu4HI GCNGC 5 cut(s) 61, 175, 223, 666, 815
FokI GGATG 1 cut(s) 106
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 5 cut(s) 61, 175, 223, 666, 815
FspBI CTAG 4 cut(s) 158, 323, 747, 884
GlaI GCGC 1 cut(s) 130
GluI GCNGC 5 cut(s) 61, 175, 223, 666, 815
GsuI CTGGAG 1 cut(s) 616
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 5 cut(s) 84, 192, 405, 449, 732
HgaI GACGC 2 cut(s) 154, 358
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 369
Hin1II CATG 6 cut(s) 67, 430, 474, 590, 672, 848
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 2 cut(s) 205, 298
HindII GTYRAC 2 cut(s) 205, 298
HindIII AAGCTT 2 cut(s) 337, 647
HinfI GANTC 4 cut(s) 143, 605, 852, 864
HpaI GTTAAC 1 cut(s) 298
Hpy166II GTNNAC 4 cut(s) 17, 205, 298, 417
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 3 cut(s) 38, 384, 747
Hpy8I GTNNAC 4 cut(s) 17, 205, 298, 417
Hpy99I CGWCG 2 cut(s) 203, 289
HpyAV CCTTC 4 cut(s) 110, 193, 439, 495
HpyCH4III ACNGT 2 cut(s) 307, 824
HpyCH4IV ACGT 3 cut(s) 261, 294, 300
HpyCH4V TGCA 5 cut(s) 476, 615, 668, 770, 814
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 323, 366, 662, 767
HpyF3I CTNAG 2 cut(s) 272, 650
HpySE526I ACGT 3 cut(s) 261, 294, 300
Hsp92I GRCGYC 1 cut(s) 369
Hsp92II CATG 6 cut(s) 67, 430, 474, 590, 672, 848
HspAI GCGC 1 cut(s) 129
KspAI GTTAAC 1 cut(s) 298
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 2 cut(s) 34, 394
LmnI GCTCC 2 cut(s) 60, 597
Lsp1109I GCAGC 2 cut(s) 652, 826
LweI GCATC 1 cut(s) 246
MaeI CTAG 4 cut(s) 158, 323, 747, 884
MaeII ACGT 3 cut(s) 261, 294, 300
MaeIII GTNAC 4 cut(s) 166, 301, 478, 606
MalI GATC 2 cut(s) 36, 396
MboI GATC 2 cut(s) 34, 394
MboII GAAGA 6 cut(s) 41, 44, 149, 161, 364, 504
MfeI CAATTG 2 cut(s) 765, 809
MhlI GDGCHC 2 cut(s) 57, 507
MlsI TGGCCA 1 cut(s) 84
MluCI AATT 3 cut(s) 737, 765, 809
MluNI TGGCCA 1 cut(s) 84
MlyI GAGTC 3 cut(s) 137, 614, 846
MmeI TCCRAC 1 cut(s) 829
Mox20I TGGCCA 1 cut(s) 84
MscI TGGCCA 1 cut(s) 84
MseI TTAA 4 cut(s) 246, 297, 695, 879
MslI CAYNNNNRTG 1 cut(s) 109
Msp20I TGGCCA 1 cut(s) 84
MspA1I CMGCKG 2 cut(s) 80, 222
MspR9I CCNGG 1 cut(s) 717
MunI CAATTG 2 cut(s) 765, 809
MvaI CCWGG 1 cut(s) 717
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 5 cut(s) 171, 323, 366, 662, 767
NcoI CCATGG 1 cut(s) 426
NdeII GATC 2 cut(s) 34, 394
NheI GCTAGC 1 cut(s) 322
NlaIII CATG 6 cut(s) 67, 430, 474, 590, 672, 848
NmuCI GTSAC 4 cut(s) 166, 301, 478, 606
NspI RCATGY 2 cut(s) 67, 474
OliI CACNNNNGTG 1 cut(s) 109
PfeI GAWTC 1 cut(s) 864
PkrI GCNGC 5 cut(s) 62, 176, 224, 667, 816
PleI GAGTC 3 cut(s) 137, 613, 846
PpsI GAGTC 3 cut(s) 137, 613, 846
Psp124BI GAGCTC 1 cut(s) 57
Psp1406I AACGTT 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 715
PspGI CCWGG 1 cut(s) 715
PspPI GGNCC 2 cut(s) 190, 731
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 109
SacI GAGCTC 1 cut(s) 57
SacII CCGCGG 1 cut(s) 223
SaqAI TTAA 4 cut(s) 246, 297, 695, 879
SatI GCNGC 5 cut(s) 61, 175, 223, 666, 815
Sau3AI GATC 2 cut(s) 34, 394
Sau96I GGNCC 2 cut(s) 190, 731
SchI GAGTC 3 cut(s) 137, 614, 846
ScrFI CCNGG 1 cut(s) 717
SduI GDGCHC 2 cut(s) 57, 507
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 1 cut(s) 306
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 109
Sse9I AATT 3 cut(s) 737, 765, 809
SsiI CCGC 8 cut(s) 58, 61, 78, 174, 208, 220, 222, 235
SspMI CTAG 4 cut(s) 158, 323, 747, 884
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 715
StyI CCWWGG 2 cut(s) 255, 426
TaaI ACNGT 2 cut(s) 307, 824
TaiI ACGT 3 cut(s) 264, 297, 303
TaqI TCGA 1 cut(s) 438
TasI AATT 3 cut(s) 737, 765, 809
TauI GCSGC 3 cut(s) 63, 177, 225
TfiI GAWTC 1 cut(s) 864
Tru1I TTAA 4 cut(s) 246, 297, 695, 879
Tru9I TTAA 4 cut(s) 246, 297, 695, 879
TscAI CASTG 3 cut(s) 310, 457, 483
TseFI GTSAC 4 cut(s) 166, 301, 478, 606
TseI GCWGC 2 cut(s) 665, 814
Tsp45I GTSAC 4 cut(s) 166, 301, 478, 606
TspDTI ATGAA 1 cut(s) 150
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 3 cut(s) 310, 457, 483
XapI RAATTY 1 cut(s) 737
XbaI TCTAGA 1 cut(s) 746
XceI RCATGY 2 cut(s) 67, 474
XspI CTAG 4 cut(s) 158, 323, 747, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.