Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0011349

Physical Location & Seq

Genomic Coordinates Forward (+)

80914482 .. 80923844

Gene Structure 9363 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022098

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGGGGATTGCTCGTTTACAAGAGAGGGAAGAAGATCACGAGCTATTACAAGAGCTCCGCCGCATGTTGGTCTCCTCCGCCGGCCAAGACAAACCACACCTTCCACAAAAGCGCTTCTCATCCAAAAGCGCTTATGAAGATGACTCGGAAGAGAAGCTAGTCTGCGTCACAAGCGGCGTTTCCTTCTTGGGCCTCGCCGTCGTCAACCGCCTCCTCCTCCGCGGCTACTCCGTCCGCATCATCGTTAATAACTCCGTGGACGTTGAAAAACTGAGGGAGATGACGACGGCGAACGTTAACGTCACCGGAGTTATGGCGAAGCTAGCTGAAGTTGAAAGCTTATCACAAGCATTTCAGGGCTGTCGTGGCGTCTTCCACACCTCTGGATTTACGGATCCTGCTGGCCTTTCTGGGTACACTAAATCCATGGCTGATATCGAAGTGAAGGCCAGTGAGAATGTGATGAGGGCATGCGCAGTGACACCTTCAGTAAGAAAATGTGTGCTCACTTCTTCACTTTTAGCCTGTGTATGGCAAAAGCAAGACATTTCACAAAATGACCTCTCCACAATCCCTGTTATCAACCATGACTGCTGGAGCAATGAGTCACTCTGCATAGAAAAAAAGCTGTGGTATGCTTTGGGGAAACTTAGAGCAGAGAAAGCTGCATGGACATTAGCCAAAGAAACTGGGTTAAAGCTCACTACAATCTGCCCAGGTCTGATTACTGGCCCTGAATTTTGCTCTAGAAACCCAACAGCGACAATTGCATATCTCAAAGGTGCCCAAGAGATGTACCAAAGTGGTGTGCTAGCAACCGTGGATGTGATGAGACTGGCAGAAGTTCATGTAAGTTTATATGAGGCGATGAACAAGTCAGCATTTGGTAGATACATTTGCTTTGATCAAGTCATTGAAACAGAGAAAGAGGCAGAGAAGCTAGCAGGGGAGACCAAGATCAAGCTTTCCCAGAACCATCAACATTCTCATTTCAGCTCCAACCAAGTTATAAAAAACGGGTCAAGTAATCCGCAACCCGAAATGGGTCACCAGAGCCCGACTCCGGTGCCACGTAAACACCACTCACCGGACTCGAGTTCATCGGACGGCGGAGCCGCCGCAATCAGCAGAGCGAAGAAGAGCCGCTACCCAGTAGACGACGACGACGACGACGACGGCGCCTGCGGTGATCTGGTCGAGTGCTCCGGCAAGTACTGCCGGTCATGCAGCGCCGGCATGATCGCCGATTGCGTGGCGCTGTGCTGCTGCCCCTGCGCTCTGGTCAACCTGTTGACTCTGGCCTTCGTGAAAGTGCCGTGGATGGTTGGGAGGAAGTGCTTGGGGTTAGGCCAGAACAAGAAGAAGAAGAAGGAAAACCAGAGCCAGGGGCAGAATCGGAAATCCAAAAAAAAGCGCAGGAGCAAGAAGAAGCAGATCAGTAGTAGTCATCGGCATCAATGCTGCGCGGCGGAGGAGGAATTGCCGGCGCAGATATCGTGCTACGTGTACGGTGTTGATGATCAGGAGGAGGAAGAAGAGGAGGATTGTGTGAGTAGTGCAGAGAGGGTATGGTTGGAATTGTACCAGGTGGGACATTTGGGTTTTGGGAGGGTTTCGTTTAGTGGTGGAGGAGTTCAGTCTGTGCAGGGGAAGGGCAATTTGGGAAACATGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009889 GO:0009890 GO:0009892 GO:0009962 GO:0009964 GO:0009987 GO:0016491 GO:0016627 GO:0016628 GO:0019222 GO:0019438 GO:0019748 GO:0033729 GO:0042742 GO:0043207 GO:0044237 GO:0044249 GO:0044550 GO:0048519 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0071704 GO:0080090 GO:0098542 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

3Beta_HSD Epimerase

Protein Analysis

559

Amino Acids

61.21

Weight (kDa)

7.71

Isoelectric Point (pI)

46.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	51 - 129	5.3e-06	NmrA-like family
Epimerase	PF01370	55 - 255	9.4e-07	NAD dependent epimerase/dehydratase family
3Beta_HSD	PF01073	56 - 185	1.4e-09	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10	PF13460	63 - 242	3.3e-06	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011349)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14700 AT5G14700
fragaria_vesca	FvH4_6g50850
malus_domestica	MD09G1031700.v1.1 MD17G1033400.v1.1
prunus_persica	Prupe.3G286200_v2.0.a1
pyrus_communis	pycom111g02510 pycom17g03060
rosa_chinensis	RchiOBHm_Chr2g0172231
rosa_laevigata	RLG00000022098
rosa_multiflora	Rmu_co8477553.1_g000001 Rmu_sc0024549.1_g000006 Rmu_sc0025855.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080040
rosa_rugosa	Rorug02G0558000
rosa_samantha	Rh2AG633100 Rh2BG646700 Rh2CG613600 Rh2DG661500
rosa_wichuraiana	Rw2G052410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1010
Acc16I	TGCGCA	1 cut(s)	475
AccB1I	GGYRCC	3 cut(s)	782, 1066, 1178
AccI	GTMKAC	1 cut(s)	1155
AccII	CGCG	2 cut(s)	222, 1466
AclI	AACGTT	1 cut(s)	294
AclWI	GGATC	2 cut(s)	389, 402
AcoI	YGGCCR	1 cut(s)	82
AcsI	RAATTY	1 cut(s)	737
AcuI	CTGAAG	2 cut(s)	348, 471
AcyI	GRCGYC	2 cut(s)	369, 1179
AfaI	GTAC	5 cut(s)	416, 797, 1214, 1508, 1583
AfeI	AGCGCT	2 cut(s)	113, 130
AfiI	CCNNNNNNNGG	6 cut(s)	67, 531, 1043, 1063, 1087, 1384
AflIII	ACRYGT	1 cut(s)	1503
AgsI	TTSAA	3 cut(s)	266, 335, 917
AjnI	CCWGG	3 cut(s)	715, 1383, 1584
AjuI	GAANNNNNNNTTGG	2 cut(s)	1643, 1675
Alw21I	GWGCWC	3 cut(s)	57, 507, 1205
Alw26I	GTCTC	3 cut(s)	76, 826, 944
AlwI	GGATC	2 cut(s)	389, 402
Ama87I	CYCGRG	1 cut(s)	1093
Aor51HI	AGCGCT	2 cut(s)	113, 130
AoxI	GGCC	7 cut(s)	82, 190, 403, 447, 730, 1299, 1348
ApeKI	GCWGC	5 cut(s)	665, 1227, 1263, 1266, 1461
ApoI	RAATTY	1 cut(s)	737
AspS9I	GGNCC	2 cut(s)	190, 731
AsuHPI	GGTGA	4 cut(s)	295, 1040, 1077, 1199
AsuNHI	GCTAGC	3 cut(s)	322, 811, 940
AvaI	CYCGRG	1 cut(s)	1093
BaeGI	GKGCMC	1 cut(s)	787
BamHI	GGATCC	1 cut(s)	394
BanI	GGYRCC	3 cut(s)	782, 1066, 1178
BanII	GRGCYC	2 cut(s)	57, 1058
BarI	GAAGNNNNNNTAC	2 cut(s)	1130, 1162
BauI	CACGAG	1 cut(s)	38
BbsI	GAAGAC	1 cut(s)	364
Bbv12I	GWGCWC	3 cut(s)	57, 507, 1205
BbvI	GCAGC	5 cut(s)	652, 1239, 1250, 1253, 1448
BccI	CCATC	2 cut(s)	984, 1315
BceAI	ACGGC	5 cut(s)	182, 303, 1123, 1192, 1300
BcgI	CGANNNNNNTGC	2 cut(s)	1048, 1082
BciT130I	CCWGG	3 cut(s)	717, 1385, 1586
BclI	TGATCA	2 cut(s)	904, 1519
BcoDI	GTCTC	3 cut(s)	76, 826, 944
BfaI	CTAG	5 cut(s)	158, 323, 747, 812, 941
BfoI	RGCGCY	5 cut(s)	115, 132, 1182, 1233, 1259
BmcAI	AGTACT	1 cut(s)	1214
Bme1390I	CCNGG	3 cut(s)	717, 1385, 1586
BmeT110I	CYCGRG	1 cut(s)	1093
BmgT120I	GGNCC	2 cut(s)	190, 731
BmiI	GGNNCC	5 cut(s)	396, 784, 1068, 1114, 1180
BmrFI	CCNGG	3 cut(s)	717, 1385, 1586
BmrI	ACTGGG	2 cut(s)	699, 1145
BmsI	GCATC	2 cut(s)	246, 1462
BmtI	GCTAGC	3 cut(s)	326, 815, 944
BmuI	ACTGGG	2 cut(s)	699, 1145
BpiI	GAAGAC	1 cut(s)	364
BplI	GAGNNNNNCTC	2 cut(s)	1045, 1077
BpmI	CTGGAG	1 cut(s)	616
BsaAI	YACGTR	2 cut(s)	1073, 1504
BsaHI	GRCGYC	2 cut(s)	369, 1179
BsaI	GGTCTC	2 cut(s)	76, 944
BsaJI	CCNNGG	7 cut(s)	220, 255, 426, 715, 819, 1316, 1384
BsaWI	WCCGGW	3 cut(s)	305, 1063, 1087
BsaXI	ACNNNNNCTCC	2 cut(s)	39, 69
Bsc4I	CCNNNNNNNGG	6 cut(s)	67, 531, 1043, 1063, 1087, 1384
Bse118I	RCCGGY	4 cut(s)	80, 1218, 1232, 1483
Bse1I	ACTGG	5 cut(s)	450, 694, 733, 840, 1151
Bse3DI	GCAATG	1 cut(s)	607
BseBI	CCWGG	3 cut(s)	717, 1385, 1586
BseDI	CCNNGG	7 cut(s)	220, 255, 426, 715, 819, 1316, 1384
BseGI	GGATG	3 cut(s)	119, 829, 1326
BseLI	CCNNNNNNNGG	6 cut(s)	67, 531, 1043, 1063, 1087, 1384
BseMI	GCAATG	1 cut(s)	607
BseMII	CTCAG	1 cut(s)	263
BseNI	ACTGG	5 cut(s)	450, 694, 733, 840, 1151
BseRI	GAGGAG	7 cut(s)	64, 203, 206, 1487, 1541, 1553, 1644
BseSI	GKGCMC	1 cut(s)	787
BseXI	GCAGC	5 cut(s)	652, 1239, 1250, 1253, 1448
BsgI	GTGCAG	2 cut(s)	1578, 1664
Bsh1236I	CGCG	2 cut(s)	222, 1466
BshFI	GGCC	7 cut(s)	84, 192, 405, 449, 732, 1301, 1350
BshNI	GGYRCC	3 cut(s)	782, 1066, 1178
BsiHKAI	GWGCWC	3 cut(s)	57, 507, 1205
BsiHKCI	CYCGRG	1 cut(s)	1093
BsiSI	CCGG	8 cut(s)	81, 306, 1064, 1088, 1206, 1219, 1233, 1484
BslFI	GGGAC	1 cut(s)	1605
BslI	CCNNNNNNNGG	6 cut(s)	67, 531, 1043, 1063, 1087, 1384
BsmAI	GTCTC	3 cut(s)	76, 826, 944
BsmFI	GGGAC	1 cut(s)	1605
BsnI	GGCC	7 cut(s)	84, 192, 405, 449, 732, 1301, 1350
Bso31I	GGTCTC	2 cut(s)	76, 944
BsoBI	CYCGRG	1 cut(s)	1093
Bsp1286I	GDGCHC	5 cut(s)	57, 507, 787, 1058, 1205
Bsp143I	GATC	8 cut(s)	34, 394, 904, 957, 1189, 1239, 1434, 1519
Bsp19I	CCATGG	1 cut(s)	426
BspANI	GGCC	7 cut(s)	84, 192, 405, 449, 732, 1301, 1350
BspCNI	CTCAG	1 cut(s)	264
BspFNI	CGCG	2 cut(s)	222, 1466
BspLI	GGNNCC	5 cut(s)	396, 784, 1068, 1114, 1180
BspOI	GCTAGC	3 cut(s)	326, 815, 944
BspPI	GGATC	2 cut(s)	389, 402
BspQI	GCTCTTC	1 cut(s)	1133
BspT107I	GGYRCC	3 cut(s)	782, 1066, 1178
BspTNI	GGTCTC	2 cut(s)	76, 944
BsrDI	GCAATG	1 cut(s)	607
BsrFI	RCCGGY	4 cut(s)	80, 1218, 1232, 1483
BsrI	ACTGG	5 cut(s)	450, 694, 733, 840, 1151
BssAI	RCCGGY	4 cut(s)	80, 1218, 1232, 1483
BssECI	CCNNGG	7 cut(s)	220, 255, 426, 715, 819, 1316, 1384
BssMI	GATC	8 cut(s)	34, 394, 904, 957, 1189, 1239, 1434, 1519
BssNI	GRCGYC	2 cut(s)	369, 1179
BssSI	CACGAG	1 cut(s)	38
BssT1I	CCWWGG	1 cut(s)	426
Bst2BI	CACGAG	1 cut(s)	38
Bst2UI	CCWGG	3 cut(s)	717, 1385, 1586
Bst4CI	ACNGT	2 cut(s)	820, 1511
Bst6I	CTCTTC	3 cut(s)	144, 1133, 1530
BstACI	GRCGYC	2 cut(s)	369, 1179
BstAPI	GCANNNNNTGC	1 cut(s)	1215
BstBAI	YACGTR	2 cut(s)	1073, 1504
BstC8I	GCNNGC	9 cut(s)	82, 324, 403, 472, 813, 942, 1183, 1234, 1485
BstDEI	CTNAG	2 cut(s)	272, 650
BstDSI	CCRYGG	5 cut(s)	220, 255, 426, 819, 1316
BstEII	GGTNACC	1 cut(s)	1046
BstF5I	GGATG	3 cut(s)	119, 829, 1326
BstFNI	CGCG	2 cut(s)	222, 1466
BstH2I	RGCGCY	5 cut(s)	115, 132, 1182, 1233, 1259
BstKTI	GATC	8 cut(s)	37, 397, 907, 960, 1192, 1242, 1437, 1522
BstMAI	GTCTC	3 cut(s)	76, 826, 944
BstMBI	GATC	8 cut(s)	34, 394, 904, 957, 1189, 1239, 1434, 1519
BstMWI	GCNNNNNNNGC	9 cut(s)	171, 323, 366, 662, 767, 1215, 1224, 1233, 1272
BstNI	CCWGG	3 cut(s)	717, 1385, 1586
BstNSI	RCATGY	2 cut(s)	67, 474
BstPI	GGTNACC	1 cut(s)	1046
BstSCI	CCNGG	3 cut(s)	715, 1383, 1584
BstSLI	GKGCMC	1 cut(s)	787
BstUI	CGCG	2 cut(s)	222, 1466
BstV1I	GCAGC	5 cut(s)	652, 1239, 1250, 1253, 1448
BstV2I	GAAGAC	1 cut(s)	364
BstX2I	RGATCY	1 cut(s)	394
BstXI	CCANNNNNNTGG	1 cut(s)	383
BstYI	RGATCY	1 cut(s)	394
BsuRI	GGCC	7 cut(s)	84, 192, 405, 449, 732, 1301, 1350
BtgI	CCRYGG	5 cut(s)	220, 255, 426, 819, 1316
BtgZI	GCGATG	1 cut(s)	881
BtsCI	GGATG	3 cut(s)	119, 829, 1326
BtsI	GCAGTG	1 cut(s)	483
BtsIMutI	CAGTG	2 cut(s)	457, 483
Cac8I	GCNNGC	9 cut(s)	82, 324, 403, 472, 813, 942, 1183, 1234, 1485
Cfr10I	RCCGGY	4 cut(s)	80, 1218, 1232, 1483
Cfr13I	GGNCC	2 cut(s)	190, 731
Cfr42I	CCGCGG	1 cut(s)	223
CseI	GACGC	2 cut(s)	154, 358
CsiI	ACCWGGT	1 cut(s)	1584
Csp6I	GTAC	5 cut(s)	415, 796, 1213, 1507, 1582
CviAII	CATG	9 cut(s)	64, 427, 471, 587, 669, 848, 1224, 1237, 1669
CviQI	GTAC	5 cut(s)	415, 796, 1213, 1507, 1582
DdeI	CTNAG	2 cut(s)	272, 650
DinI	GGCGCC	1 cut(s)	1180
DpnI	GATC	8 cut(s)	36, 396, 906, 959, 1191, 1241, 1436, 1521
DpnII	GATC	8 cut(s)	34, 394, 904, 957, 1189, 1239, 1434, 1519
EaeI	YGGCCR	1 cut(s)	82
Eam1104I	CTCTTC	3 cut(s)	144, 1133, 1530
EarI	CTCTTC	3 cut(s)	144, 1133, 1530
EciI	GGCGGA	4 cut(s)	47, 67, 1125, 1484
Ecl136II	GAGCTC	1 cut(s)	55
Eco130I	CCWWGG	1 cut(s)	426
Eco24I	GRGCYC	2 cut(s)	57, 1058
Eco31I	GGTCTC	2 cut(s)	76, 944
Eco32I	GATATC	2 cut(s)	436, 1494
Eco47III	AGCGCT	2 cut(s)	113, 130
Eco53kI	GAGCTC	1 cut(s)	55
Eco57I	CTGAAG	2 cut(s)	348, 471
Eco88I	CYCGRG	1 cut(s)	1093
Eco91I	GGTNACC	1 cut(s)	1046
EcoICRI	GAGCTC	1 cut(s)	55
EcoO65I	GGTNACC	1 cut(s)	1046
EcoRII	CCWGG	3 cut(s)	715, 1383, 1584
EcoRV	GATATC	2 cut(s)	436, 1494
EcoT14I	CCWWGG	1 cut(s)	426
EcoT38I	GRGCYC	2 cut(s)	57, 1058
EgeI	GGCGCC	1 cut(s)	1180
EheI	GGCGCC	1 cut(s)	1180
ErhI	CCWWGG	1 cut(s)	426
FaeI	CATG	9 cut(s)	67, 430, 474, 590, 672, 851, 1227, 1240, 1672
FaqI	GGGAC	1 cut(s)	1605
FatI	CATG	9 cut(s)	63, 426, 470, 586, 668, 847, 1223, 1236, 1668
FbaI	TGATCA	2 cut(s)	904, 1519
FblI	GTMKAC	1 cut(s)	1155
FokI	GGATG	3 cut(s)	106, 836, 1333
FriOI	GRGCYC	2 cut(s)	57, 1058
FspBI	CTAG	5 cut(s)	158, 323, 747, 812, 941
FspI	TGCGCA	1 cut(s)	475
GsuI	CTGGAG	1 cut(s)	616
HaeII	RGCGCY	5 cut(s)	115, 132, 1182, 1233, 1259
HaeIII	GGCC	7 cut(s)	84, 192, 405, 449, 732, 1301, 1350
HapII	CCGG	8 cut(s)	81, 306, 1064, 1088, 1206, 1219, 1233, 1484
HgaI	GACGC	2 cut(s)	154, 358
Hin1I	GRCGYC	2 cut(s)	369, 1179
Hin1II	CATG	9 cut(s)	67, 430, 474, 590, 672, 851, 1227, 1240, 1672
HincII	GTYRAC	4 cut(s)	205, 298, 1285, 1293
HindII	GTYRAC	4 cut(s)	205, 298, 1285, 1293
HindIII	AAGCTT	2 cut(s)	337, 962
HinfI	GANTC	6 cut(s)	143, 605, 1060, 1091, 1294, 1393
HpaI	GTTAAC	1 cut(s)	298
HpaII	CCGG	8 cut(s)	81, 306, 1064, 1088, 1206, 1219, 1233, 1484
HphI	GGTGA	4 cut(s)	295, 1040, 1077, 1199
Hpy188I	TCNGA	4 cut(s)	148, 723, 1105, 1398
Hpy188III	TCNNGA	5 cut(s)	38, 384, 747, 1306, 1523
Hpy99I	CGWCG	8 cut(s)	203, 289, 1163, 1166, 1169, 1172, 1175, 1178
HpyAV	CCTTC	7 cut(s)	110, 193, 439, 495, 1312, 1363, 1645
HpyCH4III	ACNGT	2 cut(s)	820, 1511
HpyCH4IV	ACGT	5 cut(s)	261, 294, 300, 1072, 1503
HpyCH4V	TGCA	6 cut(s)	615, 668, 770, 1227, 1559, 1645
HpyF10VI	GCNNNNNNNGC	9 cut(s)	171, 323, 366, 662, 767, 1215, 1224, 1233, 1272
HpyF3I	CTNAG	2 cut(s)	272, 650
HpySE526I	ACGT	5 cut(s)	261, 294, 300, 1072, 1503
Hsp92I	GRCGYC	2 cut(s)	369, 1179
Hsp92II	CATG	9 cut(s)	67, 430, 474, 590, 672, 851, 1227, 1240, 1672
KasI	GGCGCC	1 cut(s)	1178
KroI	GCCGGC	3 cut(s)	80, 1232, 1483
KroNI	GCCGGC	3 cut(s)	82, 1234, 1485
Ksp22I	TGATCA	2 cut(s)	904, 1519
KspAI	GTTAAC	1 cut(s)	298
KspI	CCGCGG	1 cut(s)	223
Kzo9I	GATC	8 cut(s)	34, 394, 904, 957, 1189, 1239, 1434, 1519
LguI	GCTCTTC	1 cut(s)	1133
LmnI	GCTCC	6 cut(s)	60, 597, 1001, 1112, 1208, 1419
Lsp1109I	GCAGC	5 cut(s)	652, 1239, 1250, 1253, 1448
LweI	GCATC	2 cut(s)	246, 1462
MabI	ACCWGGT	1 cut(s)	1584
MaeI	CTAG	5 cut(s)	158, 323, 747, 812, 941
MaeII	ACGT	5 cut(s)	261, 294, 300, 1072, 1503
MaeIII	GTNAC	5 cut(s)	166, 301, 478, 606, 1046
MalI	GATC	8 cut(s)	36, 396, 906, 959, 1191, 1241, 1436, 1521
MboI	GATC	8 cut(s)	34, 394, 904, 957, 1189, 1239, 1434, 1519
MfeI	CAATTG	1 cut(s)	765
MflI	RGATCY	1 cut(s)	394
MhlI	GDGCHC	5 cut(s)	57, 507, 787, 1058, 1205
MluCI	AATT	6 cut(s)	737, 765, 1478, 1577, 1657, 1672
Mly113I	GGCGCC	1 cut(s)	1179
MlyI	GAGTC	5 cut(s)	137, 614, 1054, 1085, 1288
MmeI	TCCRAC	2 cut(s)	1023, 1554
MroNI	GCCGGC	3 cut(s)	80, 1232, 1483
MseI	TTAA	3 cut(s)	246, 297, 695
MspA1I	CMGCKG	1 cut(s)	222
MspI	CCGG	8 cut(s)	81, 306, 1064, 1088, 1206, 1219, 1233, 1484
MspR9I	CCNGG	3 cut(s)	717, 1385, 1586
MunI	CAATTG	1 cut(s)	765
MvaI	CCWGG	3 cut(s)	717, 1385, 1586
MvnI	CGCG	2 cut(s)	222, 1466
MwoI	GCNNNNNNNGC	9 cut(s)	171, 323, 366, 662, 767, 1215, 1224, 1233, 1272
NaeI	GCCGGC	3 cut(s)	82, 1234, 1485
NarI	GGCGCC	1 cut(s)	1179
NcoI	CCATGG	1 cut(s)	426
NdeII	GATC	8 cut(s)	34, 394, 904, 957, 1189, 1239, 1434, 1519
NgoMIV	GCCGGC	3 cut(s)	80, 1232, 1483
NheI	GCTAGC	3 cut(s)	322, 811, 940
NlaIII	CATG	9 cut(s)	67, 430, 474, 590, 672, 851, 1227, 1240, 1672
NlaIV	GGNNCC	5 cut(s)	396, 784, 1068, 1114, 1180
NmuCI	GTSAC	5 cut(s)	166, 301, 478, 606, 1046
NsbI	TGCGCA	1 cut(s)	475
NspI	RCATGY	2 cut(s)	67, 474
PaeI	GCATGC	1 cut(s)	474
PaeR7I	CTCGAG	1 cut(s)	1093
PciSI	GCTCTTC	1 cut(s)	1133
PcsI	WCGNNNNNNNCGW	4 cut(s)	1164, 1167, 1170, 1248
PdiI	GCCGGC	3 cut(s)	82, 1234, 1485
PfeI	GAWTC	1 cut(s)	1393
PleI	GAGTC	5 cut(s)	137, 613, 1054, 1085, 1288
PluTI	GGCGCC	1 cut(s)	1182
PpsI	GAGTC	5 cut(s)	137, 613, 1054, 1085, 1288
Ppu21I	YACGTR	2 cut(s)	1073, 1504
PsiI	TTATAA	1 cut(s)	1010
Psp124BI	GAGCTC	1 cut(s)	57
Psp1406I	AACGTT	1 cut(s)	294
Psp6I	CCWGG	3 cut(s)	715, 1383, 1584
PspEI	GGTNACC	1 cut(s)	1046
PspGI	CCWGG	3 cut(s)	715, 1383, 1584
PspN4I	GGNNCC	5 cut(s)	396, 784, 1068, 1114, 1180
PspPI	GGNCC	2 cut(s)	190, 731
PspXI	VCTCGAGB	1 cut(s)	1093
PsuI	RGATCY	1 cut(s)	394
RsaI	GTAC	5 cut(s)	416, 797, 1214, 1508, 1583
RsaNI	GTAC	5 cut(s)	415, 796, 1213, 1507, 1582
SacI	GAGCTC	1 cut(s)	57
SacII	CCGCGG	1 cut(s)	223
SapI	GCTCTTC	1 cut(s)	1133
SaqAI	TTAA	3 cut(s)	246, 297, 695
Sau3AI	GATC	8 cut(s)	34, 394, 904, 957, 1189, 1239, 1434, 1519
Sau96I	GGNCC	2 cut(s)	190, 731
ScaI	AGTACT	1 cut(s)	1214
SchI	GAGTC	5 cut(s)	137, 614, 1054, 1085, 1288
ScrFI	CCNGG	3 cut(s)	717, 1385, 1586
SduI	GDGCHC	5 cut(s)	57, 507, 787, 1058, 1205
SexAI	ACCWGGT	1 cut(s)	1584
SfaNI	GCATC	2 cut(s)	246, 1462
SfoI	GGCGCC	1 cut(s)	1180
Sfr274I	CTCGAG	1 cut(s)	1093
Sfr303I	CCGCGG	1 cut(s)	223
SgrBI	CCGCGG	1 cut(s)	223
SlaI	CTCGAG	1 cut(s)	1093
SmlI	CTYRAG	1 cut(s)	1093
SmoI	CTYRAG	1 cut(s)	1093
SphI	GCATGC	1 cut(s)	474
Sse9I	AATT	6 cut(s)	737, 765, 1478, 1577, 1657, 1672
SspDI	GGCGCC	1 cut(s)	1178
SspMI	CTAG	5 cut(s)	158, 323, 747, 812, 941
SstI	GAGCTC	1 cut(s)	57
StyD4I	CCNGG	3 cut(s)	715, 1383, 1584
StyI	CCWWGG	1 cut(s)	426
TaaI	ACNGT	2 cut(s)	820, 1511
TaiI	ACGT	5 cut(s)	264, 297, 303, 1075, 1506
TaqI	TCGA	3 cut(s)	438, 1094, 1197
TasI	AATT	6 cut(s)	737, 765, 1478, 1577, 1657, 1672
TatI	WGTACW	1 cut(s)	1212
TauI	GCSGC	7 cut(s)	63, 177, 225, 1118, 1121, 1146, 1469
TfiI	GAWTC	1 cut(s)	1393
Tru1I	TTAA	3 cut(s)	246, 297, 695
Tru9I	TTAA	3 cut(s)	246, 297, 695
TscAI	CASTG	2 cut(s)	457, 483
TseFI	GTSAC	5 cut(s)	166, 301, 478, 606, 1046
TseI	GCWGC	5 cut(s)	665, 1227, 1263, 1266, 1461
Tsp45I	GTSAC	5 cut(s)	166, 301, 478, 606, 1046
TspDTI	ATGAA	4 cut(s)	150, 836, 884, 1089
TspGWI	ACGGA	3 cut(s)	220, 244, 407
TspRI	CASTG	2 cut(s)	457, 483
XapI	RAATTY	1 cut(s)	737
XbaI	TCTAGA	1 cut(s)	746
XceI	RCATGY	2 cut(s)	67, 474
XhoI	CTCGAG	1 cut(s)	1093
XmiI	GTMKAC	1 cut(s)	1155
XspI	CTAG	5 cut(s)	158, 323, 747, 812, 941
ZrmI	AGTACT	1 cut(s)	1214

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.