Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0011349

Physical Location & Seq

Genomic Coordinates Reverse (-)

2391778 .. 2395550

Gene Structure 3773 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1033400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGGGATTGTGGGTTTGGATGAGAGGGAGAACATCGAGTTGCAGGAGTTCTGCCGTATGCTGATGGCTTCCGCCGGCCTCCACCGCAGAAAAGACGACCGCCAGCAACGCAACAACAAGCGTTTCGACTTGAGGGGTGACGATGAAGAAGACATGTTGGTGTGTGTCACTAGCGGCGTTTCTTTCTTAGGACTTGCCCTCGTTGACCGTCTCCTCCGCCGTGGCTACTCCGTCCGCCTTGTCGTTGACAACCAAGATGTAGAAAAATTGAGGGAGATGAGGACAAGCGGCAGCACCGACGACCGCATTTCTGCAGTTATGGCGAAGCTAACCGACGATGTCGAAAGCTTATCCCATGCATTCGATGGCTGTCGTGGCGTTTTCCACACCTCTGCATTTGTCGACCCCGCTGGCCTCTCTGGCTACACTAAATCCATGGCTGAGATAGAAGTGAAGGCGAGTGAGAACGTGATGAAAGCATGTGCAGTGACACCATCTGTGAGAAAATGTGTCCTCACTTCTTCACTTCTAGCCTGCGTATGGCAGGAGAGCACCCTCAACCACCTCTCCCCTGTAATTAACCATGACTCCTGGAGCACAGAGTCACTCTGCATTGACAAAAAGCTTTGGTATGCCTTGGGGAAGCTGAGGGCCGAGAAAGCTGCATGGAAAATAGCTGAGGAAAAGGGGGTGAAGTTGGCCACCATCTGCCCAGCTCTTATTACTGGCCCTGAAATTTCCACTAGAAATCCAACAGCAACACTTGCATATCTCAAAGGAGCACAAGAAATGTACCAAAGTGGTGTGCTAGCCACAGTCGATATAACAAGACTGGCAGAGGCACATTTAGGCGTGTTTGAGGCAATGAACGAGGCAGCGTTTGGGAGATACATTTGCTTCGATCGAGTCGTCGACGGAGAAGAAGAGGCCGAAAAGTTAGCAGAGGCGACGAGCATGCCGAAGAACAAGTTTGTGGGCAATGGGGGCTCTAATATTATCCAGAATCGATTCGAATTGTCGAACAGAAAGCTTACAAATCTTTTGTCCGGAAGAGTACATTGCTGCTATAGTTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009889 GO:0009890 GO:0009892 GO:0009962 GO:0009964 GO:0009987 GO:0016491 GO:0016627 GO:0016628 GO:0019222 GO:0019438 GO:0019748 GO:0033729 GO:0042742 GO:0043207 GO:0044237 GO:0044249 GO:0044550 GO:0048519 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0071704 GO:0080090 GO:0098542 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

3Beta_HSD Epimerase

Protein Analysis

359

Amino Acids

39.61

Weight (kDa)

6.05

Isoelectric Point (pI)

37.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	55 - 253	3.6e-08	NAD dependent epimerase/dehydratase family
3Beta_HSD	PF01073	56 - 268	3.2e-11	3-beta hydroxysteroid dehydrogenase/isomerase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011349)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14700 AT5G14700
fragaria_vesca	FvH4_6g50850
malus_domestica	MD09G1031700.v1.1 MD17G1033400.v1.1
prunus_persica	Prupe.3G286200_v2.0.a1
pyrus_communis	pycom111g02510 pycom17g03060
rosa_chinensis	RchiOBHm_Chr2g0172231
rosa_laevigata	RLG00000022098
rosa_multiflora	Rmu_co8477553.1_g000001 Rmu_sc0024549.1_g000006 Rmu_sc0025855.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080040
rosa_rugosa	Rorug02G0558000
rosa_samantha	Rh2AG633100 Rh2BG646700 Rh2CG613600 Rh2DG661500
rosa_wichuraiana	Rw2G052410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	402, 912
AccIII	TCCGGA	1 cut(s)	1046
AciI	CCGC	9 cut(s)	72, 85, 100, 174, 217, 235, 288, 304, 408
AcoI	YGGCCR	1 cut(s)	699
AcsI	RAATTY	1 cut(s)	735
AfaI	GTAC	2 cut(s)	794, 1056
AfiI	CCNNNNNNNGG	1 cut(s)	540
AflIII	ACRYGT	1 cut(s)	153
AjnI	CCWGG	1 cut(s)	590
AluBI	AGCT	8 cut(s)	328, 348, 625, 646, 662, 677, 716, 1030
AluI	AGCT	8 cut(s)	328, 348, 625, 646, 662, 677, 716, 1030
Alw21I	GWGCWC	3 cut(s)	554, 599, 784
Alw26I	GTCTC	1 cut(s)	215
Aor13HI	TCCGGA	1 cut(s)	1046
AoxI	GGCC	6 cut(s)	76, 412, 651, 699, 727, 927
ApeKI	GCWGC	4 cut(s)	291, 662, 875, 1062
ApoI	RAATTY	1 cut(s)	735
ArsI	GACNNNNNNTTYG	2 cut(s)	1028, 1060
Asp700I	GAANNNNTTC	1 cut(s)	1007
AspS9I	GGNCC	2 cut(s)	651, 728
AsuHPI	GGTGA	2 cut(s)	149, 703
AsuII	TTCGAA	1 cut(s)	1011
AsuNHI	GCTAGC	1 cut(s)	808
BalI	TGGCCA	1 cut(s)	701
BanII	GRGCYC	1 cut(s)	989
BbsI	GAAGAC	1 cut(s)	156
Bbv12I	GWGCWC	3 cut(s)	554, 599, 784
BbvCI	CCTCAGC	2 cut(s)	647, 678
BbvI	GCAGC	4 cut(s)	303, 649, 887, 1049
BccI	CCATC	4 cut(s)	58, 359, 502, 713
BceAI	ACGGC	2 cut(s)	39, 204
BcgI	CGANNNNNNTGC	4 cut(s)	644, 678, 937, 971
BciT130I	CCWGG	1 cut(s)	592
BcoDI	GTCTC	1 cut(s)	215
BfaI	CTAG	4 cut(s)	171, 530, 744, 809
BfmI	CTRYAG	2 cut(s)	312, 1066
BglI	GCCNNNNNGGC	1 cut(s)	420
BisI	GCNGC	6 cut(s)	175, 289, 292, 663, 876, 1063
BlsI	GCNGC	6 cut(s)	176, 290, 293, 664, 877, 1064
Bme1390I	CCNGG	1 cut(s)	592
BmgT120I	GGNCC	2 cut(s)	651, 728
BmrFI	CCNGG	1 cut(s)	592
BmtI	GCTAGC	1 cut(s)	812
BpiI	GAAGAC	1 cut(s)	156
BpmI	CTGGAG	1 cut(s)	613
Bpu10I	CCTNAGC	2 cut(s)	647, 678
Bpu14I	TTCGAA	1 cut(s)	1011
BpuEI	CTTGAG	1 cut(s)	151
Bsa29I	ATCGAT	1 cut(s)	1006
BsaJI	CCNNGG	3 cut(s)	220, 435, 636
BsaWI	WCCGGW	1 cut(s)	1046
BsaXI	ACNNNNNCTCC	4 cut(s)	551, 572, 581, 602
Bsc4I	CCNNNNNNNGG	1 cut(s)	540
Bse118I	RCCGGY	1 cut(s)	74
Bse1I	ACTGG	2 cut(s)	730, 837
Bse3DI	GCAATG	3 cut(s)	870, 985, 1057
BseAI	TCCGGA	1 cut(s)	1046
BseBI	CCWGG	1 cut(s)	592
BseCI	ATCGAT	1 cut(s)	1006
BseDI	CCNNGG	3 cut(s)	220, 435, 636
BseGI	GGATG	1 cut(s)	25
BseLI	CCNNNNNNNGG	1 cut(s)	540
BseMI	GCAATG	3 cut(s)	870, 985, 1057
BseMII	CTCAG	3 cut(s)	432, 638, 669
BseNI	ACTGG	2 cut(s)	730, 837
BseRI	GAGGAG	1 cut(s)	203
BseXI	GCAGC	4 cut(s)	303, 649, 887, 1049
BseYI	CCCAGC	1 cut(s)	712
BsgI	GTGCAG	1 cut(s)	504
Bsh1285I	CGRYCG	3 cut(s)	100, 304, 904
BshFI	GGCC	6 cut(s)	78, 414, 653, 701, 729, 929
BshVI	ATCGAT	1 cut(s)	1006
BsiEI	CGRYCG	3 cut(s)	100, 304, 904
BsiHKAI	GWGCWC	3 cut(s)	554, 599, 784
BsiSI	CCGG	2 cut(s)	75, 1047
BslI	CCNNNNNNNGG	1 cut(s)	540
BsmAI	GTCTC	1 cut(s)	215
BsmBI	CGTCTC	1 cut(s)	215
BsmI	GAATGC	1 cut(s)	359
BsnI	GGCC	6 cut(s)	78, 414, 653, 701, 729, 929
Bsp119I	TTCGAA	1 cut(s)	1011
Bsp1286I	GDGCHC	4 cut(s)	554, 599, 784, 989
Bsp13I	TCCGGA	1 cut(s)	1046
Bsp143I	GATC	1 cut(s)	901
Bsp19I	CCATGG	1 cut(s)	435
BspACI	CCGC	9 cut(s)	72, 85, 100, 174, 217, 235, 288, 304, 408
BspANI	GGCC	6 cut(s)	78, 414, 653, 701, 729, 929
BspCNI	CTCAG	3 cut(s)	433, 639, 670
BspDI	ATCGAT	1 cut(s)	1006
BspEI	TCCGGA	1 cut(s)	1046
BspMAI	CTGCAG	1 cut(s)	316
BspOI	GCTAGC	1 cut(s)	812
BspT104I	TTCGAA	1 cut(s)	1011
BsrDI	GCAATG	3 cut(s)	870, 985, 1057
BsrFI	RCCGGY	1 cut(s)	74
BsrI	ACTGG	2 cut(s)	730, 837
BssAI	RCCGGY	1 cut(s)	74
BssECI	CCNNGG	3 cut(s)	220, 435, 636
BssMI	GATC	1 cut(s)	901
BssT1I	CCWWGG	2 cut(s)	435, 636
Bst2UI	CCWGG	1 cut(s)	592
Bst4CI	ACNGT	2 cut(s)	209, 817
Bst6I	CTCTTC	2 cut(s)	918, 1045
BstAPI	GCANNNNNTGC	1 cut(s)	764
BstBI	TTCGAA	1 cut(s)	1011
BstC8I	GCNNGC	6 cut(s)	76, 104, 412, 535, 810, 956
BstDEI	CTNAG	4 cut(s)	187, 441, 647, 678
BstDSI	CCRYGG	2 cut(s)	220, 435
BstF5I	GGATG	1 cut(s)	25
BstKTI	GATC	1 cut(s)	904
BstMAI	GTCTC	1 cut(s)	215
BstMBI	GATC	1 cut(s)	901
BstMCI	CGRYCG	3 cut(s)	100, 304, 904
BstMWI	GCNNNNNNNGC	8 cut(s)	84, 108, 320, 375, 420, 659, 764, 984
BstNI	CCWGG	1 cut(s)	592
BstNSI	RCATGY	3 cut(s)	157, 483, 958
BstSCI	CCNGG	1 cut(s)	590
BstSFI	CTRYAG	2 cut(s)	312, 1066
BstV1I	GCAGC	4 cut(s)	303, 649, 887, 1049
BstV2I	GAAGAC	1 cut(s)	156
Bsu15I	ATCGAT	1 cut(s)	1006
BsuRI	GGCC	6 cut(s)	78, 414, 653, 701, 729, 929
BsuTUI	ATCGAT	1 cut(s)	1006
BtgI	CCRYGG	2 cut(s)	220, 435
BtsCI	GGATG	1 cut(s)	25
BtsI	GCAGTG	1 cut(s)	492
BtsIMutI	CAGTG	1 cut(s)	492
Cac8I	GCNNGC	6 cut(s)	76, 104, 412, 535, 810, 956
Cfr10I	RCCGGY	1 cut(s)	74
Cfr13I	GGNCC	2 cut(s)	651, 728
ClaI	ATCGAT	1 cut(s)	1006
Csp6I	GTAC	2 cut(s)	793, 1055
CviAII	CATG	7 cut(s)	154, 356, 436, 480, 584, 666, 955
CviQI	GTAC	2 cut(s)	793, 1055
DdeI	CTNAG	4 cut(s)	187, 441, 647, 678
DpnI	GATC	1 cut(s)	903
DpnII	GATC	1 cut(s)	901
EaeI	YGGCCR	1 cut(s)	699
Eam1104I	CTCTTC	2 cut(s)	918, 1045
EarI	CTCTTC	2 cut(s)	918, 1045
EciI	GGCGGA	3 cut(s)	61, 206, 224
Eco130I	CCWWGG	2 cut(s)	435, 636
Eco24I	GRGCYC	1 cut(s)	989
EcoRII	CCWGG	1 cut(s)	590
EcoT14I	CCWWGG	2 cut(s)	435, 636
EcoT22I	ATGCAT	1 cut(s)	361
EcoT38I	GRGCYC	1 cut(s)	989
ErhI	CCWWGG	2 cut(s)	435, 636
Esp3I	CGTCTC	1 cut(s)	215
FaeI	CATG	7 cut(s)	157, 359, 439, 483, 587, 669, 958
FatI	CATG	7 cut(s)	153, 355, 435, 479, 583, 665, 954
FauI	CCCGC	1 cut(s)	415
FblI	GTMKAC	2 cut(s)	402, 912
Fnu4HI	GCNGC	6 cut(s)	175, 289, 292, 663, 876, 1063
FokI	GGATG	1 cut(s)	32
FriOI	GRGCYC	1 cut(s)	989
Fsp4HI	GCNGC	6 cut(s)	175, 289, 292, 663, 876, 1063
FspBI	CTAG	4 cut(s)	171, 530, 744, 809
GluI	GCNGC	6 cut(s)	175, 289, 292, 663, 876, 1063
GsaI	CCCAGC	1 cut(s)	716
GsuI	CTGGAG	1 cut(s)	613
HaeIII	GGCC	6 cut(s)	78, 414, 653, 701, 729, 929
HapII	CCGG	2 cut(s)	75, 1047
Hin1II	CATG	7 cut(s)	157, 359, 439, 483, 587, 669, 958
HincII	GTYRAC	4 cut(s)	205, 247, 403, 913
HindII	GTYRAC	4 cut(s)	205, 247, 403, 913
HindIII	AAGCTT	3 cut(s)	346, 623, 1028
HinfI	GANTC	5 cut(s)	587, 602, 906, 1003, 1008
HpaII	CCGG	2 cut(s)	75, 1047
HphI	GGTGA	2 cut(s)	149, 703
Hpy166II	GTNNAC	4 cut(s)	205, 247, 403, 913
Hpy188III	TCNNGA	2 cut(s)	1000, 1047
Hpy8I	GTNNAC	4 cut(s)	205, 247, 403, 913
Hpy99I	CGWCG	5 cut(s)	302, 338, 914, 917, 952
HpyAV	CCTTC	1 cut(s)	448
HpyCH4III	ACNGT	2 cut(s)	209, 817
HpyCH4IV	ACGT	1 cut(s)	468
HpyCH4V	TGCA	8 cut(s)	43, 314, 359, 395, 485, 612, 665, 767
HpyF10VI	GCNNNNNNNGC	8 cut(s)	84, 108, 320, 375, 420, 659, 764, 984
HpyF3I	CTNAG	4 cut(s)	187, 441, 647, 678
HpySE526I	ACGT	1 cut(s)	468
Hsp92II	CATG	7 cut(s)	157, 359, 439, 483, 587, 669, 958
Kpn2I	TCCGGA	1 cut(s)	1046
KroI	GCCGGC	1 cut(s)	74
KroNI	GCCGGC	1 cut(s)	76
Kzo9I	GATC	1 cut(s)	901
LmnI	GCTCC	2 cut(s)	594, 779
Lsp1109I	GCAGC	4 cut(s)	303, 649, 887, 1049
MaeI	CTAG	4 cut(s)	171, 530, 744, 809
MaeII	ACGT	1 cut(s)	468
MaeIII	GTNAC	4 cut(s)	137, 166, 487, 603
MalI	GATC	1 cut(s)	903
MboI	GATC	1 cut(s)	901
MboII	GAAGA	7 cut(s)	158, 161, 513, 932, 935, 973, 1062
MhlI	GDGCHC	4 cut(s)	554, 599, 784, 989
MlsI	TGGCCA	1 cut(s)	701
MluCI	AATT	4 cut(s)	266, 576, 735, 1013
MluNI	TGGCCA	1 cut(s)	701
MlyI	GAGTC	3 cut(s)	581, 611, 915
MmeI	TCCRAC	1 cut(s)	776
Mox20I	TGGCCA	1 cut(s)	701
Mph1103I	ATGCAT	1 cut(s)	361
MroI	TCCGGA	1 cut(s)	1046
MroNI	GCCGGC	1 cut(s)	74
MroXI	GAANNNNTTC	1 cut(s)	1007
MscI	TGGCCA	1 cut(s)	701
MseI	TTAA	1 cut(s)	579
MslI	CAYNNNNRTG	1 cut(s)	158
Msp20I	TGGCCA	1 cut(s)	701
MspA1I	CMGCKG	1 cut(s)	410
MspI	CCGG	2 cut(s)	75, 1047
MspR9I	CCNGG	1 cut(s)	592
Mva1269I	GAATGC	1 cut(s)	359
MvaI	CCWGG	1 cut(s)	592
MwoI	GCNNNNNNNGC	8 cut(s)	84, 108, 320, 375, 420, 659, 764, 984
NaeI	GCCGGC	1 cut(s)	76
NcoI	CCATGG	1 cut(s)	435
NdeII	GATC	1 cut(s)	901
NgoMIV	GCCGGC	1 cut(s)	74
NheI	GCTAGC	1 cut(s)	808
NlaIII	CATG	7 cut(s)	157, 359, 439, 483, 587, 669, 958
NmeAIII	GCCGAG	1 cut(s)	679
NmuCI	GTSAC	4 cut(s)	137, 166, 487, 603
NsiI	ATGCAT	1 cut(s)	361
NspI	RCATGY	3 cut(s)	157, 483, 958
NspV	TTCGAA	1 cut(s)	1011
PaeI	GCATGC	1 cut(s)	958
PciI	ACATGT	1 cut(s)	153
PcsI	WCGNNNNNNNCGW	2 cut(s)	906, 956
PctI	GAATGC	1 cut(s)	359
PdiI	GCCGGC	1 cut(s)	76
PdmI	GAANNNNTTC	1 cut(s)	1007
PfeI	GAWTC	2 cut(s)	1003, 1008
PflFI	GACNNNGTC	1 cut(s)	338
PfoI	TCCNGGA	1 cut(s)	590
PkrI	GCNGC	6 cut(s)	176, 290, 293, 664, 877, 1064
Ple19I	CGATCG	1 cut(s)	904
PleI	GAGTC	3 cut(s)	581, 610, 914
PpsI	GAGTC	3 cut(s)	581, 610, 914
PscI	ACATGT	1 cut(s)	153
Psp6I	CCWGG	1 cut(s)	590
PspFI	CCCAGC	1 cut(s)	712
PspGI	CCWGG	1 cut(s)	590
PspPI	GGNCC	2 cut(s)	651, 728
PstI	CTGCAG	1 cut(s)	316
PsyI	GACNNNGTC	1 cut(s)	338
PvuI	CGATCG	1 cut(s)	904
RsaI	GTAC	2 cut(s)	794, 1056
RsaNI	GTAC	2 cut(s)	793, 1055
RseI	CAYNNNNRTG	1 cut(s)	158
SalI	GTCGAC	2 cut(s)	401, 911
SaqAI	TTAA	1 cut(s)	579
SatI	GCNGC	6 cut(s)	175, 289, 292, 663, 876, 1063
Sau3AI	GATC	1 cut(s)	901
Sau96I	GGNCC	2 cut(s)	651, 728
SchI	GAGTC	3 cut(s)	581, 611, 915
ScrFI	CCNGG	1 cut(s)	592
SduI	GDGCHC	4 cut(s)	554, 599, 784, 989
SfcI	CTRYAG	2 cut(s)	312, 1066
SfuI	TTCGAA	1 cut(s)	1011
SgrDI	CGTCGACG	1 cut(s)	911
SmiMI	CAYNNNNRTG	1 cut(s)	158
SmlI	CTYRAG	1 cut(s)	130
SmoI	CTYRAG	1 cut(s)	130
SphI	GCATGC	1 cut(s)	958
Sse9I	AATT	4 cut(s)	266, 576, 735, 1013
SsiI	CCGC	9 cut(s)	72, 85, 100, 174, 217, 235, 288, 304, 408
SspI	AATATT	1 cut(s)	994
SspMI	CTAG	4 cut(s)	171, 530, 744, 809
StyD4I	CCNGG	1 cut(s)	590
StyI	CCWWGG	2 cut(s)	435, 636
TaaI	ACNGT	2 cut(s)	209, 817
TaiI	ACGT	1 cut(s)	471
TasI	AATT	4 cut(s)	266, 576, 735, 1013
TatI	WGTACW	1 cut(s)	1054
TauI	GCSGC	2 cut(s)	177, 291
TfiI	GAWTC	2 cut(s)	1003, 1008
Tru1I	TTAA	1 cut(s)	579
Tru9I	TTAA	1 cut(s)	579
TscAI	CASTG	1 cut(s)	492
TseFI	GTSAC	4 cut(s)	137, 166, 487, 603
TseI	GCWGC	4 cut(s)	291, 662, 875, 1062
Tsp45I	GTSAC	4 cut(s)	137, 166, 487, 603
TspDTI	ATGAA	4 cut(s)	159, 488, 881, 1062
TspGWI	ACGGA	2 cut(s)	220, 930
TspRI	CASTG	1 cut(s)	492
Tth111I	GACNNNGTC	1 cut(s)	338
XapI	RAATTY	1 cut(s)	735
XceI	RCATGY	3 cut(s)	157, 483, 958
XcmI	CCANNNNNNNNNTGG	1 cut(s)	362
XmiI	GTMKAC	2 cut(s)	402, 912
XmnI	GAANNNNTTC	1 cut(s)	1007
XspI	CTAG	4 cut(s)	171, 530, 744, 809
Zsp2I	ATGCAT	1 cut(s)	361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.