RchiOBHm_Chr2g0172231

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011349
Physical Location & Seq
Forward (+)
85702619 .. 85704954
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53953

Sequence Viewer

Length: 1101 bp
ATGGGGATTGCTCGTTTACAAGAGAGGGAAGAAGATCACGAGCTATTACAAGAGCTCCGCCGCATGTTGGTCTCCTCCGCTGGCCAAGACAAACCACACCTTCCACAAAAGTGCTTCTCATCCAAAAGCGCTTATGAAGATGACTCGGCAGAGAAGCTAGTCTGCGTCACAAGCGGCGTTTCCTTCTTGGGCCTCGCCGTCGTCAACCGCCTCCTCCTCCGCGGCTACTCCGTCCGCATCATCGTTAATAACTCCTTGGACGTTGAGAAACTGAGGGAGATGACGACGGCGAATGTTAACGTCACTGTAGTTATGGCGAAGCTAGCAGAAGTTGAAAGCTTATCACAAGCATTTCAGGGCTGTCGTGGCGTCTTCCACACCTCTGGATTTACCGATCCTGCTGGCCTTTCTGGGTACACTAAATCCATGGCTGATATCGAAGTGAAGGCCAGTGCGAATGTGATGAGGGCATGTGCAGTGACACCTTCAGTAAGAAAATGCGTGCTCACTTCTTCACTTTTAGCTTGTGTATGGCAAGAGCAAGACATTTCACAAAATGACCTCTCCACAATCCCTGTTATCAACCATGACTGCTGGAGCCATGAGTCACTCTGCATAGAAAAAAAGCTGTGGTATGCTTTGGGGAAACTTAGAGCAGAGAAAGCTGCATGGACATTAGCCAAAGAAACTGGGTTAAAGCTCACTACAATCTGCCCAGGTCTTATTACTGGCCCTGAATTTTTCTCTAGAAACCCAACGGCAACAATTGCATATCTCAAAGGTGCCCAAGAGATGTACCAATGTGGCGTGCTAGCAACCGTGGATGTGATGAGACTGGCAGAAGTTCATGTAAGTGTATATAAGGCGATGAACAAGTCAGCATTTGGTAGATACATTTGCTTTGATAAAGTCATTGAAACAGAGAAAGAGGCAGAGAAGCTAGCAAGGGAGACCAGTATGTCAAAGAACAAGATATGCGGAAATGATGAAGAGTCTAGTAGTGTTCATCATCTTAGATATGAATTGTCGAATAAGAAGCTTACAAATCTCTTGTCAAGAACACTAAGAAGTTGCTATAACCAAAACCAGCAATTCTATTAA

Protein Analysis

366

Amino Acids

40.77

Weight (kDa)

8.15

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 52 - 129 1.8e-07 NmrA-like family
Epimerase PF01370 55 - 258 2.6e-07 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 56 - 184 2.9e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 241 1.1e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14700 AT5G14700
fragaria_vesca FvH4_6g50850
malus_domestica MD09G1031700.v1.1 MD17G1033400.v1.1
prunus_persica Prupe.3G286200_v2.0.a1
pyrus_communis pycom111g02510 pycom17g03060
rosa_chinensis RchiOBHm_Chr2g0172231
rosa_laevigata RLG00000022098
rosa_multiflora Rmu_co8477553.1_g000001 Rmu_sc0024549.1_g000006 Rmu_sc0025855.1_g000001
rosa_roxburghii Rroxscaffold_2G00080040
rosa_rugosa Rorug02G0558000
rosa_samantha Rh2AG633100 Rh2BG646700 Rh2CG613600 Rh2DG661500
rosa_wichuraiana Rw2G052410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 958
AccB1I GGYRCC 1 cut(s) 782
AccII CGCG 1 cut(s) 222
AciI CCGC 9 cut(s) 58, 61, 78, 174, 208, 220, 222, 235, 978
AclWI GGATC 1 cut(s) 389
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 369
AfaI GTAC 2 cut(s) 416, 797
AfeI AGCGCT 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 2 cut(s) 335, 917
AjnI CCWGG 1 cut(s) 715
AleI CACNNNNGTG 1 cut(s) 109
Alw21I GWGCWC 2 cut(s) 57, 507
Alw26I GTCTC 3 cut(s) 76, 826, 944
AlwI GGATC 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 130
AoxI GGCC 5 cut(s) 82, 190, 403, 447, 730
ApeKI GCWGC 1 cut(s) 665
ApoI RAATTY 1 cut(s) 737
ArsI GACNNNNNNTTYG 2 cut(s) 1037, 1069
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 190, 731
AsuNHI GCTAGC 3 cut(s) 322, 811, 940
BaeGI GKGCMC 1 cut(s) 787
BalI TGGCCA 1 cut(s) 84
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 57
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 364
Bbv12I GWGCWC 2 cut(s) 57, 507
BbvI GCAGC 1 cut(s) 652
BceAI ACGGC 3 cut(s) 182, 303, 774
BciT130I CCWGG 1 cut(s) 717
BcoDI GTCTC 3 cut(s) 76, 826, 944
BfaI CTAG 6 cut(s) 158, 323, 747, 812, 941, 996
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 132
BisI GCNGC 4 cut(s) 61, 175, 223, 666
BlsI GCNGC 4 cut(s) 62, 176, 224, 667
Bme1390I CCNGG 1 cut(s) 717
BmgT120I GGNCC 2 cut(s) 190, 731
BmiI GGNNCC 2 cut(s) 599, 784
BmrFI CCNGG 1 cut(s) 717
BmrI ACTGGG 1 cut(s) 699
BmsI GCATC 1 cut(s) 246
BmtI GCTAGC 3 cut(s) 326, 815, 944
BmuI ACTGGG 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 616
BsaHI GRCGYC 1 cut(s) 369
BsaI GGTCTC 2 cut(s) 76, 944
BsaJI CCNNGG 5 cut(s) 220, 255, 426, 715, 819
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 5 cut(s) 450, 694, 733, 840, 954
BseBI CCWGG 1 cut(s) 717
BseDI CCNNGG 5 cut(s) 220, 255, 426, 715, 819
BseGI GGATG 2 cut(s) 119, 829
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 5 cut(s) 450, 694, 733, 840, 954
BseRI GAGGAG 3 cut(s) 64, 203, 206
BseSI GKGCMC 1 cut(s) 787
BseXI GCAGC 1 cut(s) 652
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 5 cut(s) 84, 192, 405, 449, 732
BshNI GGYRCC 1 cut(s) 782
BsiHKAI GWGCWC 2 cut(s) 57, 507
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 3 cut(s) 76, 826, 944
BsnI GGCC 5 cut(s) 84, 192, 405, 449, 732
Bso31I GGTCTC 2 cut(s) 76, 944
Bsp1286I GDGCHC 3 cut(s) 57, 507, 787
Bsp143I GATC 2 cut(s) 34, 394
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 9 cut(s) 58, 61, 78, 174, 208, 220, 222, 235, 978
BspANI GGCC 5 cut(s) 84, 192, 405, 449, 732
BspCNI CTCAG 1 cut(s) 264
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 599, 784
BspOI GCTAGC 3 cut(s) 326, 815, 944
BspPI GGATC 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 782
BspTNI GGTCTC 2 cut(s) 76, 944
BsrI ACTGG 5 cut(s) 450, 694, 733, 840, 954
BssECI CCNNGG 5 cut(s) 220, 255, 426, 715, 819
BssMI GATC 2 cut(s) 34, 394
BssNI GRCGYC 1 cut(s) 369
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 255, 426
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 2 cut(s) 307, 820
Bst6I CTCTTC 1 cut(s) 984
BstACI GRCGYC 1 cut(s) 369
BstAPI GCANNNNNTGC 1 cut(s) 767
BstC8I GCNNGC 7 cut(s) 82, 324, 403, 503, 809, 813, 942
BstDEI CTNAG 4 cut(s) 272, 650, 1013, 1064
BstDSI CCRYGG 3 cut(s) 220, 426, 819
BstF5I GGATG 2 cut(s) 119, 829
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 37, 397
BstMAI GTCTC 3 cut(s) 76, 826, 944
BstMBI GATC 2 cut(s) 34, 394
BstMWI GCNNNNNNNGC 5 cut(s) 171, 323, 366, 662, 767
BstNI CCWGG 1 cut(s) 717
BstNSI RCATGY 2 cut(s) 67, 474
BstSCI CCNGG 1 cut(s) 715
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 787
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 652
BstV2I GAAGAC 1 cut(s) 364
BstXI CCANNNNNNTGG 1 cut(s) 383
BsuRI GGCC 5 cut(s) 84, 192, 405, 449, 732
BtgI CCRYGG 3 cut(s) 220, 426, 819
BtgZI GCGATG 1 cut(s) 881
BtsCI GGATG 2 cut(s) 119, 829
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 3 cut(s) 303, 457, 483
Cac8I GCNNGC 7 cut(s) 82, 324, 403, 503, 809, 813, 942
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 190, 731
Cfr42I CCGCGG 1 cut(s) 223
CseI GACGC 2 cut(s) 154, 358
Csp6I GTAC 2 cut(s) 415, 796
CviAII CATG 7 cut(s) 64, 427, 471, 587, 602, 669, 848
CviQI GTAC 2 cut(s) 415, 796
DdeI CTNAG 4 cut(s) 272, 650, 1013, 1064
DpnI GATC 2 cut(s) 36, 396
DpnII GATC 2 cut(s) 34, 394
DrdI GACNNNNNNGTC 1 cut(s) 958
DseDI GACNNNNNNGTC 1 cut(s) 958
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 1 cut(s) 984
EarI CTCTTC 1 cut(s) 984
EciI GGCGGA 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 2 cut(s) 255, 426
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 2 cut(s) 76, 944
Eco32I GATATC 1 cut(s) 436
Eco47III AGCGCT 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 715
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 2 cut(s) 255, 426
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 255, 426
FaeI CATG 7 cut(s) 67, 430, 474, 590, 605, 672, 851
FatI CATG 7 cut(s) 63, 426, 470, 586, 601, 668, 847
Fnu4HI GCNGC 4 cut(s) 61, 175, 223, 666
FokI GGATG 2 cut(s) 106, 836
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 4 cut(s) 61, 175, 223, 666
FspBI CTAG 6 cut(s) 158, 323, 747, 812, 941, 996
GlaI GCGC 1 cut(s) 130
GluI GCNGC 4 cut(s) 61, 175, 223, 666
GsuI CTGGAG 1 cut(s) 616
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 5 cut(s) 84, 192, 405, 449, 732
HgaI GACGC 2 cut(s) 154, 358
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 369
Hin1II CATG 7 cut(s) 67, 430, 474, 590, 605, 672, 851
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 2 cut(s) 205, 298
HindII GTYRAC 2 cut(s) 205, 298
HindIII AAGCTT 2 cut(s) 337, 1037
HinfI GANTC 3 cut(s) 143, 605, 992
HpaI GTTAAC 1 cut(s) 298
Hpy166II GTNNAC 4 cut(s) 17, 205, 298, 417
Hpy188III TCNNGA 4 cut(s) 38, 384, 747, 1056
Hpy8I GTNNAC 4 cut(s) 17, 205, 298, 417
Hpy99I CGWCG 2 cut(s) 203, 289
HpyAV CCTTC 4 cut(s) 110, 193, 439, 495
HpyCH4III ACNGT 2 cut(s) 307, 820
HpyCH4IV ACGT 2 cut(s) 261, 300
HpyCH4V TGCA 4 cut(s) 476, 615, 668, 770
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 323, 366, 662, 767
HpyF3I CTNAG 4 cut(s) 272, 650, 1013, 1064
HpySE526I ACGT 2 cut(s) 261, 300
Hsp92I GRCGYC 1 cut(s) 369
Hsp92II CATG 7 cut(s) 67, 430, 474, 590, 605, 672, 851
HspAI GCGC 1 cut(s) 129
KspAI GTTAAC 1 cut(s) 298
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 2 cut(s) 34, 394
LmnI GCTCC 2 cut(s) 60, 597
Lsp1109I GCAGC 1 cut(s) 652
LweI GCATC 1 cut(s) 246
MaeI CTAG 6 cut(s) 158, 323, 747, 812, 941, 996
MaeII ACGT 2 cut(s) 261, 300
MaeIII GTNAC 4 cut(s) 166, 301, 478, 606
MalI GATC 2 cut(s) 36, 396
MboI GATC 2 cut(s) 34, 394
MboII GAAGA 6 cut(s) 41, 44, 149, 364, 504, 1001
MfeI CAATTG 1 cut(s) 765
MhlI GDGCHC 3 cut(s) 57, 507, 787
MlsI TGGCCA 1 cut(s) 84
MluCI AATT 4 cut(s) 737, 765, 1022, 1091
MluNI TGGCCA 1 cut(s) 84
MlyI GAGTC 3 cut(s) 137, 614, 1001
Mox20I TGGCCA 1 cut(s) 84
MscI TGGCCA 1 cut(s) 84
MseI TTAA 4 cut(s) 246, 297, 695, 1099
MslI CAYNNNNRTG 2 cut(s) 109, 852
Msp20I TGGCCA 1 cut(s) 84
MspA1I CMGCKG 2 cut(s) 80, 222
MspR9I CCNGG 1 cut(s) 717
MunI CAATTG 1 cut(s) 765
MvaI CCWGG 1 cut(s) 717
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 5 cut(s) 171, 323, 366, 662, 767
NcoI CCATGG 1 cut(s) 426
NdeII GATC 2 cut(s) 34, 394
NheI GCTAGC 3 cut(s) 322, 811, 940
NlaIII CATG 7 cut(s) 67, 430, 474, 590, 605, 672, 851
NlaIV GGNNCC 2 cut(s) 599, 784
NmeAIII GCCGAG 1 cut(s) 125
NmuCI GTSAC 4 cut(s) 166, 301, 478, 606
NspI RCATGY 2 cut(s) 67, 474
OliI CACNNNNGTG 1 cut(s) 109
PkrI GCNGC 4 cut(s) 62, 176, 224, 667
PleI GAGTC 3 cut(s) 137, 613, 1000
PpsI GAGTC 3 cut(s) 137, 613, 1000
Psp124BI GAGCTC 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 715
PspGI CCWGG 1 cut(s) 715
PspN4I GGNNCC 2 cut(s) 599, 784
PspPI GGNCC 2 cut(s) 190, 731
RsaI GTAC 2 cut(s) 416, 797
RsaNI GTAC 2 cut(s) 415, 796
RseI CAYNNNNRTG 2 cut(s) 109, 852
SacI GAGCTC 1 cut(s) 57
SacII CCGCGG 1 cut(s) 223
SaqAI TTAA 4 cut(s) 246, 297, 695, 1099
SatI GCNGC 4 cut(s) 61, 175, 223, 666
Sau3AI GATC 2 cut(s) 34, 394
Sau96I GGNCC 2 cut(s) 190, 731
SchI GAGTC 3 cut(s) 137, 614, 1001
ScrFI CCNGG 1 cut(s) 717
SduI GDGCHC 3 cut(s) 57, 507, 787
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 1 cut(s) 306
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 109, 852
Sse9I AATT 4 cut(s) 737, 765, 1022, 1091
SsiI CCGC 9 cut(s) 58, 61, 78, 174, 208, 220, 222, 235, 978
SspMI CTAG 6 cut(s) 158, 323, 747, 812, 941, 996
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 715
StyI CCWWGG 2 cut(s) 255, 426
TaaI ACNGT 2 cut(s) 307, 820
TaiI ACGT 2 cut(s) 264, 303
TaqI TCGA 2 cut(s) 438, 1028
TasI AATT 4 cut(s) 737, 765, 1022, 1091
TauI GCSGC 3 cut(s) 63, 177, 225
Tru1I TTAA 4 cut(s) 246, 297, 695, 1099
Tru9I TTAA 4 cut(s) 246, 297, 695, 1099
TscAI CASTG 3 cut(s) 310, 457, 483
TseFI GTSAC 4 cut(s) 166, 301, 478, 606
TseI GCWGC 1 cut(s) 665
Tsp45I GTSAC 4 cut(s) 166, 301, 478, 606
TspDTI ATGAA 6 cut(s) 150, 836, 884, 995, 1002, 1035
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 3 cut(s) 310, 457, 483
XapI RAATTY 1 cut(s) 737
XbaI TCTAGA 1 cut(s) 746
XceI RCATGY 2 cut(s) 67, 474
XspI CTAG 6 cut(s) 158, 323, 747, 812, 941, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.