AT5G16080

Steryl acetyl hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009064
Physical Location & Seq
Reverse (-)
5252251 .. 5253790
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16080.1

Sequence Viewer

Length: 1035 bp
ATGGCAACCATTTCCTTCTCTCACAACCACCAATCCTCCGACAACCGCCGTGGTGGATCCCATCACCACCGTCACGGCCCCGTCGTGGAAGAGATCGAAGGACTCATCAAAGTTTTCAACGACGGCTGCGTCGAAAGACCTCCCATAGTTCCCATCGTCTCACCAACCATTCACCCATCTTCTAAAGCCACAGCCTTTGACATCAAACTATCCAACGACACATGGACACGTGTCTACATCCCCGATGCCGCAGCAGCCTCGCCGTCCGTTACTCTCCCTCTCCTCGTTTACTTCCACGGCGGTGGTTTCTGCGTTGGCTCCGCCGCTTGGAGTTGCTACCATGACTTCTTGACCAGCCTTGCCGTTAAAGCCCGTTGCGTCATCGTTTCCGTAAACTACCGTTTAGCCCCTGAACACCGTCTCCCGGCGGCGTACGACGACGGCGTTAATGTCGTCTCGTGGCTAGTCAAGCAACAAATATCTACCGGTGGTGGATATCCTTCTTGGTTGAGTAAATGTAACCTCTCAAACGTTTTCTTGGCCGGAGATAGCGCCGGAGCCAACATAGCTTACCAAGTCGCCGTAAGAATCATGGCCAGTGGCAAATACGCAAACACTCTACACCTCAAGGGCATTATTCTCATCCACCCTTTCTTCGGCGGCGAATCAAGAACGTCTTCGGAGAAACAACAACATCACACCAAGTCATCGGCGCTTACTCTTTCAGCTTCCGACGCATATTGGCGTCTAGCTCTGCCACGTGGAGCGAGTAGAGACCATCCATGGTGTAACCCTCTGATGAGCTCGGCGGGAGCGAAGTTACCGACGACGATGGTGTTCATGGCGGAGTTTGATATACTAAAAGAACGAAACTTGGAGATGTGTAAAGTGATGAGAAGCCATGGGAAGAGAGTTGAAGGTATAGTTCATGGAGGTGTTGGTCACGCTTTTCATATTCTCGACAACTCGTCGGTGTCACGTGATCGAATCCACGACATGATGTGTCGTCTTCACAACTTCATACATCCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

344

Amino Acids

37.7

Weight (kDa)

8.99

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 78 - 200 2.1e-08 Carboxylesterase family
BD-FAE PF20434 89 - 195 1.3e-09 BD-FAE
Abhydrolase_3 PF07859 95 - 318 6.2e-66 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AccI GTMKAC 1 cut(s) 234
AciI CCGC 9 cut(s) 46, 249, 300, 321, 324, 428, 660, 809, 845
AclI AACGTT 1 cut(s) 531
AclWI GGATC 2 cut(s) 51, 64
AcoI YGGCCR 2 cut(s) 540, 594
AcvI CACGTG 3 cut(s) 230, 761, 980
AcyI GRCGYC 1 cut(s) 745
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 4 cut(s) 85, 327, 424, 656
AflIII ACRYGT 2 cut(s) 227, 229
AgeI ACCGGT 1 cut(s) 485
AgsI TTSAA 2 cut(s) 118, 917
AhdI GACNNNNNGTC 1 cut(s) 967
AleI CACNNNNGTG 1 cut(s) 300
AloI GAACNNNNNNTCC 2 cut(s) 405, 437
AluBI AGCT 4 cut(s) 569, 728, 752, 804
AluI AGCT 4 cut(s) 569, 728, 752, 804
Alw21I GWGCWC 1 cut(s) 806
Alw26I GTCTC 4 cut(s) 163, 425, 460, 768
AlwI GGATC 2 cut(s) 51, 64
AoxI GGCC 3 cut(s) 76, 540, 594
ApeKI GCWGC 3 cut(s) 126, 251, 254
AsiGI ACCGGT 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 676
AspLEI GCGC 2 cut(s) 554, 715
AspS9I GGNCC 1 cut(s) 77
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 3 cut(s) 56, 153, 164
BalI TGGCCA 1 cut(s) 596
BamHI GGATCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 806
BauI CACGAG 1 cut(s) 457
BbrPI CACGTG 3 cut(s) 230, 761, 980
BbsI GAAGAC 2 cut(s) 669, 1001
Bbv12I GWGCWC 1 cut(s) 806
BbvI GCAGC 3 cut(s) 113, 263, 266
BccI CCATC 5 cut(s) 69, 161, 184, 786, 826
BceAI ACGGC 8 cut(s) 33, 91, 139, 247, 313, 347, 457, 566
BcgI CGANNNNNNTGC 2 cut(s) 240, 274
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 4 cut(s) 163, 425, 460, 768
BfaI CTAG 2 cut(s) 464, 749
BfoI RGCGCY 2 cut(s) 555, 716
BisI GCNGC 7 cut(s) 127, 249, 252, 255, 324, 429, 661
BlsI GCNGC 7 cut(s) 128, 250, 253, 256, 325, 430, 662
Bme1390I CCNGG 1 cut(s) 425
BmeRI GACNNNNNGTC 1 cut(s) 967
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 4 cut(s) 58, 79, 319, 559
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 1 cut(s) 235
BoxI GACNNNNGTC 1 cut(s) 230
BpiI GAAGAC 2 cut(s) 669, 1001
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 425
BsaAI YACGTR 3 cut(s) 230, 761, 980
BsaHI GRCGYC 1 cut(s) 745
BsaI GGTCTC 1 cut(s) 768
BsaJI CCNNGG 4 cut(s) 49, 295, 782, 901
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 4 cut(s) 20, 50, 405, 435
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 327, 424, 656
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 1 cut(s) 597
BseDI CCNNGG 4 cut(s) 49, 295, 782, 901
BseGI GGATG 4 cut(s) 237, 642, 778, 1024
BseLI CCNNNNNNNGG 4 cut(s) 85, 327, 424, 656
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 3 cut(s) 113, 263, 266
BshFI GGCC 3 cut(s) 78, 542, 596
BshTI ACCGGT 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 806
BsiSI CCGG 4 cut(s) 425, 486, 543, 555
BsiWI CGTACG 1 cut(s) 432
BslI CCNNNNNNNGG 4 cut(s) 85, 327, 424, 656
BsmAI GTCTC 4 cut(s) 163, 425, 460, 768
BsmBI CGTCTC 3 cut(s) 163, 425, 460
BsnI GGCC 3 cut(s) 78, 542, 596
Bso31I GGTCTC 1 cut(s) 768
Bsp1286I GDGCHC 1 cut(s) 806
Bsp143I GATC 3 cut(s) 56, 93, 982
Bsp19I CCATGG 2 cut(s) 782, 901
BspACI CCGC 9 cut(s) 46, 249, 300, 321, 324, 428, 660, 809, 845
BspANI GGCC 3 cut(s) 78, 542, 596
BspLI GGNNCC 4 cut(s) 58, 79, 319, 559
BspPI GGATC 2 cut(s) 51, 64
BspTNI GGTCTC 1 cut(s) 768
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 4 cut(s) 49, 295, 782, 901
BssMI GATC 3 cut(s) 56, 93, 982
BssNI GRCGYC 1 cut(s) 745
BssSI CACGAG 1 cut(s) 457
BssT1I CCWWGG 2 cut(s) 782, 901
Bst2BI CACGAG 1 cut(s) 457
Bst4CI ACNGT 3 cut(s) 71, 401, 419
Bst6I CTCTTC 2 cut(s) 84, 902
BstACI GRCGYC 1 cut(s) 745
BstBAI YACGTR 3 cut(s) 230, 761, 980
BstDSI CCRYGG 4 cut(s) 49, 295, 782, 901
BstF5I GGATG 4 cut(s) 237, 642, 778, 1024
BstH2I RGCGCY 2 cut(s) 555, 716
BstHHI GCGC 2 cut(s) 554, 715
BstKTI GATC 3 cut(s) 59, 96, 985
BstMAI GTCTC 4 cut(s) 163, 425, 460, 768
BstMBI GATC 3 cut(s) 56, 93, 982
BstMWI GCNNNNNNNGC 5 cut(s) 254, 368, 469, 566, 734
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 423
BstV1I GCAGC 3 cut(s) 113, 263, 266
BstV2I GAAGAC 2 cut(s) 669, 1001
BstX2I RGATCY 1 cut(s) 56
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 3 cut(s) 78, 542, 596
BtgI CCRYGG 4 cut(s) 49, 295, 782, 901
BtsCI GGATG 4 cut(s) 237, 642, 778, 1024
BtsIMutI CAGTG 1 cut(s) 604
CfoI GCGC 2 cut(s) 554, 715
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 1 cut(s) 77
CseI GACGC 4 cut(s) 118, 367, 734, 743
Csp6I GTAC 1 cut(s) 433
CspAI ACCGGT 1 cut(s) 485
CspCI CAANNNNNGTGG 4 cut(s) 31, 66, 178, 213
CviAII CATG 8 cut(s) 222, 341, 592, 783, 841, 902, 929, 997
CviQI GTAC 1 cut(s) 433
DpnI GATC 3 cut(s) 58, 95, 984
DpnII GATC 3 cut(s) 56, 93, 982
DrdI GACNNNNNNGTC 1 cut(s) 128
DriI GACNNNNNGTC 1 cut(s) 967
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 2 cut(s) 540, 594
Eam1104I CTCTTC 2 cut(s) 84, 902
Eam1105I GACNNNNNGTC 1 cut(s) 967
EarI CTCTTC 2 cut(s) 84, 902
EciI GGCGGA 2 cut(s) 310, 860
Ecl136II GAGCTC 1 cut(s) 804
Eco130I CCWWGG 2 cut(s) 782, 901
Eco24I GRGCYC 1 cut(s) 806
Eco31I GGTCTC 1 cut(s) 768
Eco32I GATATC 1 cut(s) 497
Eco53kI GAGCTC 1 cut(s) 804
Eco72I CACGTG 3 cut(s) 230, 761, 980
EcoICRI GAGCTC 1 cut(s) 804
EcoRV GATATC 1 cut(s) 497
EcoT14I CCWWGG 2 cut(s) 782, 901
EcoT38I GRGCYC 1 cut(s) 806
ErhI CCWWGG 2 cut(s) 782, 901
Esp3I CGTCTC 3 cut(s) 163, 425, 460
FaeI CATG 8 cut(s) 225, 344, 595, 786, 844, 905, 932, 1000
FalI AAGNNNNNCTT 2 cut(s) 661, 693
FatI CATG 8 cut(s) 221, 340, 591, 782, 840, 901, 928, 996
FauI CCCGC 1 cut(s) 802
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 7 cut(s) 127, 249, 252, 255, 324, 429, 661
FokI GGATG 4 cut(s) 224, 629, 765, 1011
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 7 cut(s) 127, 249, 252, 255, 324, 429, 661
FspBI CTAG 2 cut(s) 464, 749
GlaI GCGC 2 cut(s) 553, 714
GluI GCNGC 7 cut(s) 127, 249, 252, 255, 324, 429, 661
HaeII RGCGCY 2 cut(s) 555, 716
HaeIII GGCC 3 cut(s) 78, 542, 596
HapII CCGG 4 cut(s) 425, 486, 543, 555
HgaI GACGC 4 cut(s) 118, 367, 734, 743
HhaI GCGC 2 cut(s) 554, 715
Hin1I GRCGYC 1 cut(s) 745
Hin1II CATG 8 cut(s) 225, 344, 595, 786, 844, 905, 932, 1000
Hin6I GCGC 2 cut(s) 552, 713
HinP1I GCGC 2 cut(s) 552, 713
HinfI GANTC 4 cut(s) 102, 588, 665, 987
HpaII CCGG 4 cut(s) 425, 486, 543, 555
HphI GGTGA 3 cut(s) 56, 153, 164
Hpy166II GTNNAC 3 cut(s) 235, 289, 394
Hpy188I TCNGA 4 cut(s) 40, 682, 733, 798
Hpy188III TCNNGA 3 cut(s) 349, 669, 959
Hpy8I GTNNAC 3 cut(s) 235, 289, 394
Hpy99I CGWCG 9 cut(s) 86, 125, 134, 440, 443, 737, 829, 832, 973
HpyAV CCTTC 4 cut(s) 25, 92, 510, 911
HpyCH4III ACNGT 3 cut(s) 71, 401, 419
HpyCH4IV ACGT 5 cut(s) 229, 531, 674, 760, 979
HpyF10VI GCNNNNNNNGC 5 cut(s) 254, 368, 469, 566, 734
HpySE526I ACGT 5 cut(s) 229, 531, 674, 760, 979
Hsp92I GRCGYC 1 cut(s) 745
Hsp92II CATG 8 cut(s) 225, 344, 595, 786, 844, 905, 932, 1000
HspAI GCGC 2 cut(s) 552, 713
Kzo9I GATC 3 cut(s) 56, 93, 982
LmnI GCTCC 4 cut(s) 323, 557, 764, 812
LpnPI CCDG 7 cut(s) 367, 423, 438, 499, 556, 568, 610
Lsp1109I GCAGC 3 cut(s) 113, 263, 266
LweI GCATC 1 cut(s) 235
MaeI CTAG 2 cut(s) 464, 749
MaeII ACGT 5 cut(s) 229, 531, 674, 760, 979
MaeIII GTNAC 7 cut(s) 71, 268, 518, 788, 819, 941, 975
MalI GATC 3 cut(s) 58, 95, 984
MboI GATC 3 cut(s) 56, 93, 982
MboII GAAGA 6 cut(s) 101, 171, 646, 669, 919, 1001
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 806
MlsI TGGCCA 1 cut(s) 596
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 3 cut(s) 63, 237, 756
MnlI CCTC 9 cut(s) 46, 150, 268, 288, 293, 533, 635, 804, 926
Mox20I TGGCCA 1 cut(s) 596
MroXI GAANNNNTTC 1 cut(s) 676
MscI TGGCCA 1 cut(s) 596
MseI TTAA 2 cut(s) 366, 447
MslI CAYNNNNRTG 2 cut(s) 300, 933
Msp20I TGGCCA 1 cut(s) 596
MspI CCGG 4 cut(s) 425, 486, 543, 555
MspR9I CCNGG 1 cut(s) 425
MwoI GCNNNNNNNGC 5 cut(s) 254, 368, 469, 566, 734
NciI CCSGG 1 cut(s) 425
NcoI CCATGG 2 cut(s) 782, 901
NdeII GATC 3 cut(s) 56, 93, 982
NlaIII CATG 8 cut(s) 225, 344, 595, 786, 844, 905, 932, 1000
NlaIV GGNNCC 4 cut(s) 58, 79, 319, 559
NmeAIII GCCGAG 1 cut(s) 785
NmuCI GTSAC 3 cut(s) 71, 941, 975
OliI CACNNNNGTG 1 cut(s) 300
PcsI WCGNNNNNNNCGW 5 cut(s) 81, 126, 129, 441, 812
PdmI GAANNNNTTC 1 cut(s) 676
PfeI GAWTC 3 cut(s) 588, 665, 987
Pfl23II CGTACG 1 cut(s) 432
PinAI ACCGGT 1 cut(s) 485
PkrI GCNGC 7 cut(s) 128, 250, 253, 256, 325, 430, 662
PleI GAGTC 1 cut(s) 96
PmaCI CACGTG 3 cut(s) 230, 761, 980
PmlI CACGTG 3 cut(s) 230, 761, 980
PpsI GAGTC 1 cut(s) 96
Ppu21I YACGTR 3 cut(s) 230, 761, 980
PshAI GACNNNNGTC 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 806
Psp1406I AACGTT 1 cut(s) 531
PspCI CACGTG 3 cut(s) 230, 761, 980
PspLI CGTACG 1 cut(s) 432
PspN4I GGNNCC 4 cut(s) 58, 79, 319, 559
PspPI GGNCC 1 cut(s) 77
PsuI RGATCY 1 cut(s) 56
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 2 cut(s) 300, 933
SacI GAGCTC 1 cut(s) 806
SaqAI TTAA 2 cut(s) 366, 447
SatI GCNGC 7 cut(s) 127, 249, 252, 255, 324, 429, 661
Sau3AI GATC 3 cut(s) 56, 93, 982
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 1 cut(s) 806
SfaNI GCATC 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 300, 933
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
SsiI CCGC 9 cut(s) 46, 249, 300, 321, 324, 428, 660, 809, 845
SspMI CTAG 2 cut(s) 464, 749
SstI GAGCTC 1 cut(s) 806
StyD4I CCNGG 1 cut(s) 423
StyI CCWWGG 2 cut(s) 782, 901
TaaI ACNGT 3 cut(s) 71, 401, 419
TaiI ACGT 5 cut(s) 232, 534, 677, 763, 982
TaqI TCGA 4 cut(s) 96, 132, 960, 985
TauI GCSGC 4 cut(s) 251, 326, 431, 663
TfiI GAWTC 3 cut(s) 588, 665, 987
Tru1I TTAA 2 cut(s) 366, 447
Tru9I TTAA 2 cut(s) 366, 447
TscAI CASTG 1 cut(s) 604
TseFI GTSAC 3 cut(s) 71, 941, 975
TseI GCWGC 3 cut(s) 126, 251, 254
Tsp45I GTSAC 3 cut(s) 71, 941, 975
TspDTI ATGAA 4 cut(s) 829, 917, 941, 1009
TspGWI ACGGA 2 cut(s) 256, 379
TspRI CASTG 1 cut(s) 604
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 676
XspI CTAG 2 cut(s) 464, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.